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See detailA novel gene overexpressed in the prostate of castrated rats: hormonal regulation, relationship to apoptosis and to acquired prostatic cell androgen independence.
Bruyninx, M.; Hennuy, Benoît ULg; Cornet, Anne ULg et al

in Endocrinology (1999), 140(10), 4789-99

We have identified a novel complementary DNA (cDNA) corresponding to a gene overexpressed in the rat ventral prostate after castration. This cDNA displays 89.4% identity with 453 bp of a mouse EST and 81 ... [more ▼]

We have identified a novel complementary DNA (cDNA) corresponding to a gene overexpressed in the rat ventral prostate after castration. This cDNA displays 89.4% identity with 453 bp of a mouse EST and 81.5% identity with 157 bp of a human EST and was named PARM-1 for prostatic androgen-repressed message-1. The complete cDNA is 1187 bp long and codes for a protein of 298 amino acids that contains four potential glycosylation sites and three half cystinyl residues. The PARM-1 gene was found to be expressed at quite low levels in most rat tissues including those of the urogenital tract. The kinetic of induction of PARM-1 gene in the prostate was highly correlated to the development of apoptosis in the whole organ. Supplementation of castrated animals with androgens reversed both the process of apoptosis and the overexpression of PARM-1 gene. Supplementation with estrogens did not result in an increase in the PARM-1 messenger RNA levels when compared with the castration alone. However, the treatment resulted in a more rapid return to intact levels in the castrated plus estrogen group. When apoptosis of testis and prostate was induced in vivo by hypophysectomy, it was found that PARM-1 was only overexpressed in the prostate. Therefore, PARM-1 seems to be regulated by androgens only in the prostate. Using in situ hybridization and immunohistological techniques, we have shown that PARM-1 gene product is found exclusively in the epithelial cells of involuting prostate. Analysis by flow cytometry of MAT LyLu epithelial cells transiently expressing PARM-1 protein did not allow us to demonstrate a direct effect of PARM-1 gene overexpression on the programmed death of the transfected cells. Treatment of MAT LyLu cells by transforming growth factor-beta induced apoptosis but had no effect on PARM-1 production. However PARM-1 protein has been detected by Western blotting in various cell lines such as MAT LyLu, MAT Lu, and PIF, which are androgen independent. This would suggest that PARM-1 gene product would be a marker for acquired androgen-independence of these tumor cells. [less ▲]

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See detailA novel glycoprotein of the aspartic proteinase gene family expressed in bovine placental trophectoderm
Xie, Sancai; Low, Boon G.; Nagel, Robert J. et al

in Biology of Reproduction (1994), 51(6), 1145-1153

The pregnancy associated glycoproteins (PAG 1) that appear in the maternal serum of cattle and sheep soon after implantation are apparently inactive members of the aspartic proteinase family. Here we ... [more ▼]

The pregnancy associated glycoproteins (PAG 1) that appear in the maternal serum of cattle and sheep soon after implantation are apparently inactive members of the aspartic proteinase family. Here we describe the isolation of a highly abundant cDNA (PAG 2 cDNA) that represents a second member of this gene family which is structurally related to bovine PAG 1, ovine PAG 1, and pepsin (58%, 58%, and 51% amino acid sequence identity, respectively). The bovine PAG 2 cDNA was identified in two ways. First, the bovine placental library was screened under relatively nonstringent conditions with an ovine PAG 1 cDNA. The second fortuitous approach employed immunoscreening with an antiserum raised against a partially purified factor that competed with bovine LH for binding to the LH receptor on the CL of the ovary. The full-length cDNA (1258 bp) codes for a polypeptide of 376 amino acids. Bovine PAG 2, unlike bovine PAG 1, has a catalytic center with a consensus sequence of amino acids. Its mRNA is expressed in fetal placenta but not in other fetal organs, and is localized to both the mononucleate and binucleate cells of the trophectoderm, whereas PAG 1 is expressed only in binucleate cells. PAG 2 is synthesized by placental explants as a 70-kDa glycoprotein that is processed to several smaller molecules. Western blot analysis of culture media developed with epitope-selected antibodies to PAG 2 reveals several bands ranging in apparent M(r) from 31,000-70,000, which correspond in size to the polypeptides present in the preparation used for immunization [less ▲]

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See detailNovel graft copolymers by organometallic-mediated radical polymerization of a conjugated with a non-conjugated monomer
Hurtgen, Marie ULg; Debuigne, Antoine ULg; Fustin, Charles-André et al

Scientific conference (2011, February 24)

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See detailNovel HDAC/DNMT twin inhibitors interfere with angiogenesis
Shiva Shankar, Thammadihalli Veerasangaiah ULg; Sulka, Béatrice; Blacher, Silvia ULg et al

Poster (2010)

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA ... [more ▼]

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA methyltransferases (DNMT) inhibitors are potent antiangiogenic compounds. Though combination of HDAC and DNMT inhibitors are now being examined in clinical trials of hematological malignancies, very little work has been done to understand the effect of this combination on normal and tumoral angiogenesis. We have designed and tested a family of twin drugs with intrinsic HDAC and DNMT inhibitory activities in relevant models of angiogenesis in vitro (endothelial cells, pericytes and the 3D aortic ring assay) and in vivo (the chick chorioallantoic membrane assay). We have identified a lead compound having quantifiable antiangiogenic effect without cytotoxicity associated with increased global acetylation and decreased DNA methylation levels. This compound is presently used to develop effective approaches to treat cancer by modulating the process of angiogenesis. [less ▲]

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See detailNovel HDAC/DNMT Twin Inhibitors Interfere with Angiogenesis
Shiva Shankar, Thammadihalli Veerasangaiah ULg; Sulka, Béatrice ULg; Blacher, Silvia ULg et al

Poster (2011)

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA ... [more ▼]

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA methyltransferases (DNMT) inhibitors are potent anti-angiogenic compounds. Though combination of HDAC and DNMT inhibitors are now being examined in clinical trials of hematological malignancies, little work has been done to understand the effect of this combination on physiological and tumoral angiogenesis. We have designed and tested a family of twin drugs with intrinsic HDAC and DNMT inhibitory activities in relevant models of angiogenesis in vitro (Human Umbilical Vein Endothelial Cells – HUVEC and aortic ring) and in vivo (chick chorioallantoic membrane and Zebrafish). We have identified a lead compound having quantifiable anti-angiogenic effect without cytotoxicity affecting global histone acetylation and DNA methylation levels. In order to elucidate its anti-angiogenic mechanism, we characterized gene expression pattern simultaneously with the methylation profile of HUVEC cells treated with the lead compound and reference epigenetic modulators. This approach based on parallel microarray analyses permitted us to underscore a list of genes exclusively affected by the lead compound but not by other HDAC or DNMT inhibitors. These genes were then analyzed using the Ingenuity Pathway software revealing potential involvement of a subset of genes in angiogenesis. Our present aim is to validate the expression levels of a series of genes with respect to epigenetic mechanisms (histone modifications and DNA methylation). Finally, the biological relevance of the target genes will be explored by RNA silencing. Hence, we are using these novel epigenetic modulators as a tool to understand the regulatory mechanism of angiogenesis and to develop effective approaches to treat cancer. [less ▲]

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See detailNovel HDAC/DNMT Twin inhibitors interfere with angiogenesis
Shiva Shankar, Thammadihalli Veerasangaiah ULg; Sulka, Béatrice ULg; Blacher, Silvia ULg et al

Poster (2011, January 31)

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA ... [more ▼]

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA methyltransferases (DNMT) inhibitors are potent anti-angiogenic compounds. Though combination of HDAC and DNMT inhibitors are now being examined in clinical trials of hematological malignancies, little work has been done to understand the effect of this combination on physiological and tumoral angiogenesis. We have designed and tested a family of twin drugs with intrinsic HDAC and DNMT inhibitory activities in relevant models of angiogenesis in vitro (Human Umbilical Vein Endothelial Cells – HUVEC and aortic ring) and in vivo (chick chorioallantoic membrane and Zebrafish). We have identified a lead compound having quantifiable anti-angiogenic effect without cytotoxicity affecting global histone acetylation and DNA methylation levels. In order to elucidate its anti-angiogenic mechanism, we characterized gene expression pattern simultaneously with the methylation profile of HUVEC cells treated with the lead compound and reference epigenetic modulators. This approach based on parallel microarray analyses permitted us to underscore a list of genes exclusively affected by the lead compound but not by other HDAC or DNMT inhibitors. These genes were then analyzed using the Ingenuity Pathway software revealing potential involvement of a subset of genes in angiogenesis. Our present aim is to validate the expression levels of a series of genes with respect to epigenetic mechanisms (histone modifications and DNA methylation). Finally, the biological relevance of the target genes will be explored by RNA silencing. Hence, we are using these novel epigenetic modulators as a tool to understand the regulatory mechanism of angiogenesis and to develop effective approaches to treat cancer. [less ▲]

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See detailNovel Higgs decay signals in R-parity violating models
Aristizabal Sierra, Diego ULg; Porod, W.; Restrepo, D. et al

in Physical Review. D : Particles and Fields (2008), 78

In supersymmetric models the lightest Higgs boson may decay with a sizable branching ratio into a pair of light neutralinos. We analyze such decays within the context of the minimal supersymmetric ... [more ▼]

In supersymmetric models the lightest Higgs boson may decay with a sizable branching ratio into a pair of light neutralinos. We analyze such decays within the context of the minimal supersymmetric standard model with R-parity violation, where the neutralino itself is unstable and decays into Standard Model fermions. We show that the R-parity violating couplings induce novel Higgs decay signals that might facilitate the discovery of the Higgs boson at colliders. At the LHC, the Higgs may be observed, for instance, through its decay -via two neutralinos- into final states containing missing energy and isolated charged leptons such as l±l∓,l±l±,3l, and 4l. Another promising possibility is the search for the displaced vertices associated with the neutralino decay. We also point out that Higgs searches at the LHC might additionally provide the first evidence of R-parity violation. [less ▲]

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See detailA novel high efficiency, low maintenance, hydroponic system for synchronous growth and flowering of Arabidopsis thaliana.
Tocquin, Pierre ULg; Corbesier, Laurent; Havelange, Andrée ULg et al

in BMC Plant Biology (2003), 3(2), 302003

Background: Arabidopsis thaliana is now the model organism for genetic and molecular plant studies, but growing conditions may still impair the significance and reproducibility of the experimental ... [more ▼]

Background: Arabidopsis thaliana is now the model organism for genetic and molecular plant studies, but growing conditions may still impair the significance and reproducibility of the experimental strategies developed. Besides the use of phytotronic cabinets, controlling plant nutrition may be critical and could be achieved in hydroponics. The availability of such a system would also greatly facilitate studies dealing with root development. However, because of its small size and rosette growth habit, Arabidopsis is hardly grown in standard hydroponic devices and the systems described in the last years are still difficult to transpose at a large scale. Our aim was to design and optimize an up-scalable device that would be adaptable to any experimental conditions. Results: An hydroponic system was designed for Arabidopsis, which is based on two units: a seed-holder and a 1-L tank with its cover. The original agar-containing seed-holder allows the plants to grow from sowing to seed set, without transplanting step and with minimal waste. The optimum nitrate supply was determined for vegetative growth, and the flowering response to photoperiod and vernalization was characterized to show the feasibility and reproducibility of experiments extending over the whole life cycle. How this equipment allowed to overcome experimental problems is illustrated by the analysis of developmental effects of nitrate reductase deficiency in nia1nia2 mutants. Conclusion: The hydroponic device described in this paper allows to drive small and large scale cultures of homogeneously growing Arabidopsis plants. Its major advantages are its flexibility, easy handling, fast maintenance and low cost. It should be suitable for many experimental purposes. [less ▲]

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See detailNovel high-resolution micro-CT based surface roughness measurement protocol for complex porous structures
Kerckhofs, Greet ULg; Pyka, Grzegorz; Moesen, Maarten et al

in Proceedings MetFoam 2011, Hur, B., Kim, S., Hyun, S. (Eds.) (2011)

Detailed reference viewed: 12 (4 ULg)
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See detailNovel high-resolution micro-CT based surface roughness measurement protocol for complex porous structures
Kerckhofs, Greet ULg; Pyka, Grzegorz; Braem, Annabel et al

in Proceedings CellMat 2010 (2010)

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See detailNovel ibandronate regimens in postmenopausal osteoporosis: design of the dosing intravenous administration (DIVA) study
Sambrook, P.; Reginster, Jean-Yves ULg; Recker, R. R. et al

in Osteoporosis International (2004, May), 15(Suppl.1), 118

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See detailA novel image-analysis toolbox enabling quantitative analysis of root system architecture
Lobet, Guillaume ULg; Pagès, L.; Draye, X.

in Plant Physiology (2011), 157(1), 29--39

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See detailNovel inhibitors of the sodium-calcium exchanger: benzene ring analogues of N-guanidino substituted amiloride derivatives
Rogister, F.; Laeckmann, D.; Plasman, P.-O. et al

in European Journal of Medicinal Chemistry (2001), 36

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See detailNovel insights into the management of atypical myopathy in grazing horses based on recent series of European outbreaks and advances in etiological investigations
Votion, Dominique ULg; Gerber, Vinzent

in Proceedings of The 12th Congress of The World Equine Veterinary (2011, November 02)

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See detailNovel Instrumentation for the Measurement of the Unsteady Pressure Distribution around a Wing Undergoing Stall Flutter Oscillations
Li, Jing; Dimitriadis, Grigorios ULg; Gu, Fengshu et al

in Autumn Conference of the Institute of Acoustics 2007: Advances in Noise and Vibration Engineering (2007, October)

In this work, a wind tunnel model of a wing undergoing stall flutter oscillations is studied. It is a rectangular wing with pitch and plunge degrees of freedom and low pitch stiffness. The objective of ... [more ▼]

In this work, a wind tunnel model of a wing undergoing stall flutter oscillations is studied. It is a rectangular wing with pitch and plunge degrees of freedom and low pitch stiffness. The objective of this study is to promote the understanding of the stall flutter phenomenon by measuring the unsteady pressure distribution around the wing as well as the wing displacement, during unforced motion in two degrees of freedom. Both steady and unsteady pressures must be measured with sufficient accuracy during two types of tests. In the static tests the wing is to be clamped in position and not allowed to move and the steady pressures are to be measured around the centre-span section at different angles of attack. Thus the stall angle of attack can be identified and the stall mechanism characterized. In the dynamic tests the wing will be allowed to move and the unsteady pressures will be measured and recorded during a number of cycles of the oscillation, at a number of free stream airspeeds. [less ▲]

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See detailNovel large ring 1,3 bridged 2-azetidinones as potential inhibitors of penicillin-binding proteins
Urbach, Allan; Dive, Georges ULg; Marchand-Brynaert, Jacqueline

in European Journal of Organic Chemistry (2009), 11

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See detailA novel lox/Cre-mediated multiple gene knockout procedue in the yeast Yarrowia lipolytica
Fickers, P.; Le Dal, M.; Gaillardin, C. et al

Poster (2003, May 09)

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See detailNovel macromolecular architectures based on aliphatic polyesters: relevance of the "coordination-insertion" ring-opening polymerization
Mecerreyes, David; Jérôme, Robert ULg; Dubois, Philippe ULg

in Advances in Polymer Science (1999), 147

Recent developments in the macromolecular engineering of aliphatic polyesters have been overviewed. First, aluminum alkoxides mediated living ring opening polymerization (ROP) of cyclic (di)esters, i.e ... [more ▼]

Recent developments in the macromolecular engineering of aliphatic polyesters have been overviewed. First, aluminum alkoxides mediated living ring opening polymerization (ROP) of cyclic (di)esters, i.e., lactones, lactides, glycolide, is introduced. An insight into this so-called "coordination-insertion" mechanism and the ability of this living polymerization process to prepare well-defined homopolymers, telechelic polymers, random and block copolymers is then discussed. In the second part, the combination of the living ROP of (di)lac-tones with other well-controlled polymerization mechanisms such as anionic, cationic, free radical, and metathesis polyadditions of unsaturated comonomers, as well as polyconden-sations, is reported with special emphasis on the design of new and well-tailored macromolecular architectures. As a result of the above synthetic breakthrough, a variety of novel materials have been developed with versatile applications in very different fields such as biomedical and microelectronics. [less ▲]

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