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See detailLaminin and fibronectin increase the steady state level of the 67 kD high affinity metastasis-associated laminin receptor mRNA in human cancer cells.
Castronovo, Vincenzo ULg; Sobel, M. E.

in Biochemical and Biophysical Research Communications (1990), 168(3), 1110-7

Among the various known laminin binding proteins, the 67 kD high affinity laminin receptor (LR) is intimately involved during tumor invasion and metastasis. In this study, we report that laminin and ... [more ▼]

Among the various known laminin binding proteins, the 67 kD high affinity laminin receptor (LR) is intimately involved during tumor invasion and metastasis. In this study, we report that laminin and fibronectin, two attachment glycoproteins, significantly increased the total cellular level of 67 kD LR mRNA in two human cancer cell lines, T47D breast carcinoma cells and A2058 melanoma cells. Neither GRGDS nor YIGSR synthetic peptides induced such a stimulatory effect. Since the steady state level of LR mRNA has been shown to control the number of receptors expressed at the cell surface, these results suggest that contact of the cancer cells with laminin and fibronectin in the host matrix may be an important regulatory mechanism by which cancer cells maintain a high number of LR at their cell surface as they progress through the several steps of tumoral invasion and metastasis. [less ▲]

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See detailLaminin and Type Iii Procollagen Peptide in Human Preovulatory Follicular Fluid
Christiane, Y.; Demoulin, A.; Gillain, D. et al

in Fertility and Sterility (1988), 50(1), 48-51

The levels of laminin P1 fragment, a marker of basement membrane, and of the aminoterminal sequence of type III procollagen, a marker of interstitial connective tissue, were measured in human preovulatory ... [more ▼]

The levels of laminin P1 fragment, a marker of basement membrane, and of the aminoterminal sequence of type III procollagen, a marker of interstitial connective tissue, were measured in human preovulatory follicular fluids. The concentrations of these peptides correlated with progesterone levels but not with those of estradiol or testosterone. Immunocytochemical studies confirmed the remodeling of the perifollicular basement membrane and interstitial matrix during oocyte maturation. The studies suggest that monitoring of the ovarian connective tissue macromolecules could be useful for estimating follicular maturation. [less ▲]

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See detailLaminin binding and internalization by human and murine mammary gland cell lines in vitro.
Coopman, P. J.; Nuydens, R.; Leunissen, J. et al

in European Journal of Cell Biology (1991), 56(2), 251-9

We have studied the binding and internalization of Engelbreth-Holm-Swarm mouse sarcoma laminin labeled with colloidal gold (LN-G40) by human and murine mammary gland cell lines. Interactions between the ... [more ▼]

We have studied the binding and internalization of Engelbreth-Holm-Swarm mouse sarcoma laminin labeled with colloidal gold (LN-G40) by human and murine mammary gland cell lines. Interactions between the LN-G40 probe and the cells spread on a glass coverslip were monitored with video-enhanced contrast microscopy (Nanovid). Transmission electron microscopy allowed the quantitation of the LN-G40 probe at various cellular locations. During the first 15 min, a homogeneous binding of LN-G40 probe to the cell surface was observed with all cell lines. This binding did not occur with gold particles that were not conjugated to laminin. Then, the LN-G40 probe began to cluster on the cell surface and was, during the following 20 h, internalized by pits that were not coated. In the cells, the LN-G40 probe sometimes showed saltatory movements along linear tracks. The LN-G40 probe was intracellularly found in vesicles, multivesicular bodies, cisternal structures, and lysosomes, suggesting the degradation of the internalized laminin. However, not all cell surface-bound LN-G40 probe was internalized after 20 h. Differences between the cell lines were quantitative, but no clear correlation could be made between migration of cells on laminin and internalization of laminin. [less ▲]

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See detailLaminin promotes cerebellar granule cells migration in vitro and is synthesized by cultured astrocytes
SELAK, Ivan ULg; Foidart, Jean-Michel ULg; Moonen, Gustave ULg

in Developmental Neuroscience (1985), 7(5-6), 278-285

Newborn rat cerebellum microexplants have been used as a model to study neuronal migration. Laminin in a substrate-bound form modifies extensively the migratory behavior of the neurones, an effect which ... [more ▼]

Newborn rat cerebellum microexplants have been used as a model to study neuronal migration. Laminin in a substrate-bound form modifies extensively the migratory behavior of the neurones, an effect which is blocked if antilaminin antibodies are present during the assay. 35S methionine incorporation followed by SDS-PAGE electrophoresis, fluorography and immunoprecipitation with antilaminin antibodies allowed to demonstrate that laminin is synthesized and secreted by cultured newborn rat cerebellum as well as by cultured newborn rat cerebral cortex astrocytes. [less ▲]

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See detailLaminin receptor complementary DNA-deduced synthetic peptide inhibits cancer cell attachment to endothelium.
Castronovo, Vincenzo ULg; Taraboletti, G.; Sobel, M. E.

in Cancer Research (1991), 51(20), 5672-8

Stable attachment of cancer cells to the endothelium is a key step in the formation of metastasis. In this study, we have investigated the possibility that interaction between laminin and its Mr 67,000 ... [more ▼]

Stable attachment of cancer cells to the endothelium is a key step in the formation of metastasis. In this study, we have investigated the possibility that interaction between laminin and its Mr 67,000 high-affinity receptor (67 LR) could play a major role in this process. Scatchard analysis of laminin-binding studies showed that bovine aortic endothelial cells exhibit 46,000 high-affinity receptors that mediate, at least in part, the attachment of highly invasive melanoma cells. This endothelial cell-melanoma cell interaction was significantly inhibited by soluble laminin and by anti-laminin antibodies. Peptide G, an eicosapeptide derived from the complementary DNA sequence of the 67 LR precursor (IPCNNKGAHSVGLMWWMLAR) that specifically binds to laminin and presumably contains the active ligand-binding site of the receptor, specifically prevented attachment of the melanoma cells to both the bovine aortic endothelial cell monolayer and human umbilical vein endothelium. Thus, peptide G may selectively interfere with the metastatic cascade by inhibiting tumor cell attachment to endothelium via the laminin-67 LR pathway and is a potential new antimetastatic agent. [less ▲]

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See detailLaminin Receptors and Laminin-Binding Proteins During Tumor Invasion and Metastasis
Castronovo, Vincenzo ULg

in Invasion & Metastasis (1993), 13(1), 1-30

Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions ... [more ▼]

Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions are mediated through a large variety of cell surface proteins designated as laminin receptors and/or laminin-binding proteins. The growing number of identified laminin binding proteins and domains of this glycoprotein that are biologically active illustrate the complexity of cellular interactions with laminin. The 67-kD laminin receptor (67 LR) was the first protein identified in 1983 as a high-affinity laminin receptor, and its expression is dramatically increased in a large variety of cancer cells. The 67 LR is the subject of several controversies and confusion generated mainly by two events. First, the identification of several new laminin-binding proteins has raised the difficult task of attributing specific functions to specific receptors in contrast to initial beliefs that all cellular laminin-driven biological activities were mediated through the 67 LR. The second source of controversy is the large molecular-weight discrepancy between the 37-kD polypeptide encoded by the 67 LR cDNA clone and the mature 67 LR. In this manuscript, a critical and extensive review of the data accumulated on the 67 LR is presented regarding both its molecular structure and its role during tumor invasion and metastasis. A hypothetical model of the 67 LR is also proposed. Since the first identification of the 67 LR, at least 14 other cell surface molecules have been reported to be potential laminin receptors or laminin-binding proteins. These include members of the beta-galactoside-binding lectin family, seven members of the integrin family, the galactosyltransferase and some not yet fully characterized cell surface molecules. These laminin receptors and laminin-binding proteins are also described and their functions are also discussed with a particular emphasis on their participation in the constitution of the invasive phenotype. [less ▲]

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See detailLaminin--a glycoprotein from basement membranes.
Timpl, R.; Rhode, H.; Robey, P. G. et al

in Journal of Biological Chemistry (1979), 254(19), 9933-7

We have isolated a large noncollagenous glycoprotein, laminin, from a mouse tumor that produces basement membrane. The protein consists of at least two polypeptide chains (Mr = 220,000 and Mr = 440,000 ... [more ▼]

We have isolated a large noncollagenous glycoprotein, laminin, from a mouse tumor that produces basement membrane. The protein consists of at least two polypeptide chains (Mr = 220,000 and Mr = 440,000) joined to each other by disulfide bonds. Laminin and type IV collagen are major constituents of the tumor. Laminin is distinctly different from fibronectin, another component of basement membranes, in amino acid composition and immunological reactivity. Pepsin digestion of laminin releases a large, cystine-rich fragment which retains most of the antigenicity of the original protein. Immunological studies using purified antibody against laminin show that it is produced by a variety of cultured cells. In addition, these antibodies react with the basement membranes of normal tissues, suggesting that this protein or an immunologically related protein is a constituent of the basement membranes of these tissues. [less ▲]

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See detailLamost Observations in the Kepler Field. I. Database of Low-resolution Spectra
De Cat, P.; Fu, J. N.; Ren, A. B. et al

in Astrophysical Journal Supplement Series (2015), 220

The nearly continuous light curves with micromagnitude precision provided by the space mission Kepler are revolutionizing our view of pulsating stars. They have revealed a vast sea of low-amplitude ... [more ▼]

The nearly continuous light curves with micromagnitude precision provided by the space mission Kepler are revolutionizing our view of pulsating stars. They have revealed a vast sea of low-amplitude pulsation modes that were undetectable from Earth. The long time base of Kepler light curves allows for the accurate determination of the frequencies and amplitudes of pulsation modes needed for in-depth asteroseismic modeling. However, for an asteroseismic study to be successful, the first estimates of stellar parameters need to be known and they cannot be derived from the Kepler photometry itself. The Kepler Input Catalog provides values for the effective temperature, surface gravity, and metallicity, but not always with sufficient accuracy. Moreover, information on the chemical composition and rotation rate is lacking. We are collecting low-resolution spectra for objects in the Kepler field of view with the Large Sky Area Multi-Object Fiber Spectroscopic Telescope (lamost, Xinglong observatory, China). All of the requested fields have now been observed at least once. In this paper, we describe those observations and provide a useful database for the whole astronomical community. ) located at the Xinglong observatory, China. [less ▲]

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See detailLa lamotrigine (Lamictal).
Scheen, André ULg

in Revue Médicale de Liège (1997), 52(11), 738-40

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See detailLamotrigine kinetics within the menstrual cycle, after menopause, and with oral contraceptives.
Wegner, Ilse; Edelbroek, Peter M.; BULK, Saskia ULg et al

in Neurology (2009), 73(17), 1388-93

OBJECTIVE: We prospectively evaluated the fluctuation of lamotrigine (LTG) clearance during the menstrual cycle. We also assessed the effect of postmenopausal status and investigated in detail the effect ... [more ▼]

OBJECTIVE: We prospectively evaluated the fluctuation of lamotrigine (LTG) clearance during the menstrual cycle. We also assessed the effect of postmenopausal status and investigated in detail the effect of oral contraceptives (OCs) on LTG clearance. METHODS: Three groups of women with epilepsy using LTG monotherapy were evaluated. Women in the first group (n = 7) had a regular cycle and did not use OCs; the second group used a 1-phase combined OC (n = 7), and the third group (n = 7) was postmenopausal. Two menstrual cycles or at least 2 months (postmenopausal women) were assessed, monitoring LTG levels every other day. RESULTS: The mean apparent LTG clearance in women of reproductive age not using OCs was 49 (SD 22.6, range 20.4-83.5) L/24 hours. No significant effect of endogenous hormones on LTG clearance was found. In women using OCs, the mean LTG clearance was 126 (SD 60.2, range 44.3-205) L/24 hours. There was an increase in LTG levels during the pill-free week, with maximum levels 54% (range 29%-129%) higher than baseline levels. LTG levels decreased to the baseline value within a mean of 8 days of starting OC use (SD 3.7, range 2.5-16.5). In the postmenopausal women, the mean clearance was 82 (SD 38.4, range 35.9-125) L/24 hours. CONCLUSIONS: We observed a higher mean lamotrigine (LTG) clearance in postmenopausal women compared with young women not using oral contraceptives (OCs) and confirmed that OC use may have a strong effect on LTG clearance. There was no significant fluctuation of LTG clearance during the menstrual cycle. [less ▲]

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See detailLAN Switch Security: What Hackers Know About Your Switches
Vyncke, Eric ULg; Paggen, Christophe

Book published by CiscoPress (2006)

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See detailLancement d’un programme d’amélioration génétique de Jatropha curcas L. au Sénégal
Terren, Marieke ULg; Saverys, S.; Jacquet de Haverskercke, P et al

Poster (2009)

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See detailLancement d’une unité de cogénération biomasse de 30 kWel sur le marché
Oudkerk, Jean-François ULg

Master's dissertation (2012)

La cogénération est une technique de production rationnelle et efficace de l’énergie. Le principe est simple : produire de l’électricité et de la chaleur simultanément. Le but étant d’éviter les pertes ... [more ▼]

La cogénération est une technique de production rationnelle et efficace de l’énergie. Le principe est simple : produire de l’électricité et de la chaleur simultanément. Le but étant d’éviter les pertes d’énergie thermique inévitablement liées aux centrales électriques de grosse puissance. Cette technique permet d’économiser de l’énergie primaire, de réduire les émissions de CO2 et de générer un revenu pour l’exploitant. Pour ses divers avantages, elle est mise en avant par la Commission européenne comme technologie à développer pour atteindre les objectifs énergétiques de l’Union Européenne. Depuis quelques années, la micro cogénération arrive sur le marché. Malgré les estimations prometteuses, elle n’a pas encore réussi à atteindre le marché de masse comme l’ont fait certaines technologies relatives à la production d’énergie verte pour le particulier (on pense notamment au succès des panneaux photovoltaïques). Mais les experts s’accordent à dire que, désormais, tous les facteurs sont réunis pour que le marché de la cogénération se développe comme il le mérite. Ce travail commence par analyser les facteurs qui influencent le marché de la cogénération et tente d’expliquer pourquoi celui-ci n’en est qu’à sa phase de lancement. L’analyse de ces facteurs montre que le marché a connu un début difficile mais qu’aujourd’hui, tout est en place pour qu’il entre dans sa phase de croissance. Le but de cette démarche est d’évaluer le potentiel commercial d’un produit innovant proposé par CORETEC. Afin de déterminer ce potentiel, un modèle de la rentabilité économique d’une unité de cogénération est proposé. Ce modèle se base sur un profil de consommation de chaleur horaire pour déterminer la production d’énergie et la consommation en combustible, heure par heure, de la cogénération tout au long d’une année. Connaissant la production et la consommation, il est possible de déterminer la rentabilité du projet via trois critères : le temps de retour actualisé, la valeur actuelle nette et le taux de rentabilité interne. Ce modèle est utilisé pour déterminer le client cible en Région wallonne. Ce dernier est le client pour qui le produit offre une rentabilité économique. Après avoir détermine le profil du client cible, un nombre de clients potentiels est déterminé sur base de statistiques énergétiques. Pour terminer, une comparaison avec un produit standard du marché est effectuée et l’influence des mécanismes de soutien mis en place par la Région wallonne est étudiée. [less ▲]

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See detailLand availability and housing development: the case of Wallonia
Halleux, Jean-Marie ULg; Strée, Julie ULg

Conference (2013, May 24)

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See detailLand conversion for industrialization and its impacts on household livelihood strategies in Hung Yen province, northern Vietnam
Nguyen, Thi Dien ULg

Doctoral thesis (2011)

This thesis is about the impact of land conversion for industrialization on household livelihood strategies in Hung Yen province, northern Vietnam. The study shows that land conversion is generating a ... [more ▼]

This thesis is about the impact of land conversion for industrialization on household livelihood strategies in Hung Yen province, northern Vietnam. The study shows that land conversion is generating a household landholdings decline while boosting the land market. After land conversion, 16% of laborers in the surveyed households find employment in industrial factories while 52% find jobs in the informal sector. Because of land conversion, rather than being net food producers, peasant households have become net food purchasers. Meantime, food safety in industrial areas is threatened by increasing environmental pollution. The findings also demonstrate that 51.9% of the surveyed households choose diversification as a livelihood strategy while 35.6% of them shift entirely to non-farm strategies. Among the affected peasant groups, households with a non-farm background that lost less than 50% of their agricultural land are likely to be in a better position to engage in lucrative non-farm jobs. Land conversion is causing a complex agrarian transformation in Vietnam. On the one hand, it generates a mechanism of social differentiation which is determined by land alteration and capital accumulation from high earning non-farm activities. On the other hand, peasant livelihood strategies, thanks to adaptation and innovation, have mitigated the impacts of land conversion and reveal the persistence of a peasant economy. [less ▲]

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See detailLand conversion to industrialization and its impacts on household food security in Red River Delta, Vietnam
Nguyen Thi, Dien; Lebailly, Philippe ULg; Vu Dinh, Ton

Conference (2010)

Since 1990s Vietnam is undergoing rapid industrialization with the national goal of becoming an industrial country. As a result, large tracts of agricultural land were conversed to industrial zones and ... [more ▼]

Since 1990s Vietnam is undergoing rapid industrialization with the national goal of becoming an industrial country. As a result, large tracts of agricultural land were conversed to industrial zones and clusters. This paper analyzes the complex impacts caused by industrialization on household food security. Both qualitative and quantitative methods were applied to investigate how land conversion process to industrial companies affected food security of different groups of peasant households in Hung Yen province, the most rapid industrialization province in Red River Delta of Vietnam. The study showed that industrialization and neo-liberalism affected household food security in three aspects: the lost of large agricultural land area which did not ensure stable jobs for peasants; the decline of living quality as a result of environmental pollution and high living costs; and the lost of household self reliance on food, with 77 percent of surveyed households not producing enough food for their own consumption. Industrialization had created a new class structure in the Vietnamese countryside with the rise of landless peasants who survived by selling their labor and who are net food buyers. The study contributes to the research that link the analysis of household food security with livelihood systems in the processes interacting across scales, from the very local to the global. [less ▲]

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See detailLand Cover Dynamics (1990-2002) in Binh Thuan Province, Southern Central Vietnam
Hountondji, Yvon ULg; De Longueville, Florence ULg; Ozer, Pierre ULg

in International Journal of Asian Social Science (2012), 2(3), 336-349

This paper describes the use of satellite imageries and GIS data for identifying key environmental characteristics of Binh Thuan Province in south central Vietnam and for detecting the major changes ... [more ▼]

This paper describes the use of satellite imageries and GIS data for identifying key environmental characteristics of Binh Thuan Province in south central Vietnam and for detecting the major changes patterns within this region. Landsat TM (1990) and Landsat ETM+ (2002) imageries were used to classify the study area into seven land use and land cover (LULC) classes. A post-classification comparison analysis was used to quantify and illustrate the various LULC conversions that took place over the 12-year span of time. Results showed that a steady growth in population has caused extensive changes of land cover throughout the area. The maps also indicate that the loss of woody land (forest) and the extension of wetlands (irrigated area), combined with built-up encroachment, remains one of the most serious environmental problems facing the Binh Thuan Province today. The post-classification change detection analysis showed that critical habitats accounted for nearly 38.5% of the intensive study area between 1990 and 2002 while 61.5% remained stable. Results also showed over the 12-year span, approximately 1151.2 km² (115.120 ha) forest were converted respectively to brush, irrigated area (wetlands), cropland and built-up. This is an overall average decrease of 9594 hectares of forested area per year. Throughout the study area, districts most affected by forest conversion to another land cover are: Bac Bihn (2798 ha/year), Than Linh (2717 ha/year), Ham Thuan Nam (1601 ha/year) and Ham Thuan Bac (1524 ha/year). Based on the identified causes of these changes, we made policy recommendations for better management of land use and land cover. [less ▲]

Detailed reference viewed: 105 (18 ULg)