Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailInsight into the role of catalases in salt stress in potato (Solanum tuberosum L.).
M'Hamdi, M.; Bettaieb, T.; Harbaoui, Y. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailInsight on Van Dongen’s La Violoniste by hyperspectral imaging and MA-XRF
Herens, Elodie ULg; Strivay, David ULg; Walter, Philippe et al

Conference (2016, July 06)

Detailed reference viewed: 27 (8 ULg)
Full Text
Peer Reviewed
See detailInsight on variables leading to burnout in cancer physicians
Bragard, Isabelle ULg; Libert, Y.; Etienne, Anne-Marie ULg et al

in Journal of Cancer Education : The Official Journal of the American Association for Cancer Education (2010), 25

Detailed reference viewed: 42 (5 ULg)
Full Text
Peer Reviewed
See detailInsights gained from metagenomic sequencing of apple fruit surface (cv. Pinova)
Angeli, Dario; Massart, Sébastien ULg; Sare, Abdoul Razack ULg et al

Poster (2016, May 17)

Plant microbial communities (microbiota) living at the surface of fruit have been the source of the majority of biocontrol agents (BCAs). Despite this interest, their role as a community has been poorly ... [more ▼]

Plant microbial communities (microbiota) living at the surface of fruit have been the source of the majority of biocontrol agents (BCAs). Despite this interest, their role as a community has been poorly studied so far. They holistic study was an unattainable objective due to the absence of techniques to survey microbial communities and their evolution. The recent developments in high-throughput sequencing (HTS) have now enabled the holistic analysis of the microbiota genomes. A pioneering assay has been carried out to get insight into the microbiota of apple surface through metagenome sequencing. Apple from Pinova cultivar have been sampled in orchard and the microbiota isolated. After DNA extraction, the HTS assay generated 14.5 Gbases which were assembled in 133,888 contigs. These contigs provided useful information on taxonomic composition of the microbiota. A total of 1398 bacterial species and 397 fungal species have been identified. Among them, 22 species corresponded to known apple pathogens (Penicillium expansum, Penicillium spp, Botrytis cinerea, Monilinia fructicola, Nectria haematococca, Glomerella cingulate, G. graminicola, Alternaria alternate, Alternaria brassicicola, A. citri, A. longipes, A. solani, A. tenuissima, Fusarium solani, F. oxysporum, F. culmorum, F. lateritium, Mucor circinelloides, M. hiemalis, F. racemosus, Venturia inaequalis, Cladosporium cladosporioides). In addition, several contigs were assigned to species or genus of known BCA strains like Pichia canadensis, Bacillus sp., Debaryomyces hansenii, Aureobasidium pullulans and Pantoea vagans. These results will be discussed. In any case, these results underline a very diverse microbial community whose role needs to be characterized. In this line, the functional analysis (gene function determination) of the contigs is ongoing. [less ▲]

Detailed reference viewed: 93 (5 ULg)
Full Text
Peer Reviewed
See detailInsights into a million-year-scale Rhenohercynian carbonate platform evolution through a multi-disciplinary approach: example of a GIvetian carbonate record from Belgium
Pas, Damien ULg; Da Silva, Anne-Christine ULg; Devleeschouwer, Xavier et al

in Geological Magazine (2016), 154(4), 707-739

In this paper we formulate answers to three important questions related to Givetian carbonate records and their use for reconstructing million-year past palaeoenvironmental changes. First, we provide ... [more ▼]

In this paper we formulate answers to three important questions related to Givetian carbonate records and their use for reconstructing million-year past palaeoenvironmental changes. First, we provide detailed illustrations of the fascinating diversity that shaped a significant shallow reefal platform during the early- to late-Givetian in the Rhenohercynian Ocean; secondly we improve the sedimentological model of the extensive Givetian carbonate platform in the Dinant Basin and thirdly we evaluate the application of magnetic susceptibility (MS) as a tool for long-term trend correlations and palaeoenvironmental reconstructions. These goals are reached by making sedimentological, geophysical and geochemical study of the La Thure section. Through the early-late Givetian interval we discerned eighteen microfacies ranging from a homoclinal ramp to a discontinuously rimmed shelf and then a drowning shelf. The comparison of these sedimentological results with those published in the south of the Dinant Syncline allowed us to provide an up to date model of the vertical and lateral environmental development for one of the largest Givetian carbonate platforms in Europe. This comparison also increased the knowledge on the facies belts distribution into the Dinant Basin and allowed us to highlight the Taghanic Event. Palaeo-redox proxies reveal a substantial change in the oxygenation level, from oxygen-depleted to more oxic condition, between middle and late Givetian. We demonstrated the relationship between variation in MS values and proxies for siliciclastic input (such as Si, Al). The La Thure is considered as a key section for the understanding of internal shelf settings bordering Laurussia’s south-eastern margin. [less ▲]

Detailed reference viewed: 90 (4 ULg)
Full Text
Peer Reviewed
See detailInsights into a million-year-scale Rhenohercynian carbonate platform evolution through a multi-disciplinary approach: example of a GIvetian carbonate record from Belgium
Pas, Damien ULg; Da Silva, Anne-Christine ULg; Devleeschouwer, Xavier et al

Poster (2016)

In this study we formulate answers to three important questions related to Givetian carbonate records and their use for reconstructing million-year past palaeoenvironmental changes. First, we provide ... [more ▼]

In this study we formulate answers to three important questions related to Givetian carbonate records and their use for reconstructing million-year past palaeoenvironmental changes. First, we provide detailed illustrations of the fascinating depositional diversity that shaped a shallow reefal platform during the early- to late-Givetian in the Rhenohercynian Ocean; secondly we improve the sedimentological model of the Givetian carbonate platform in the Dinant Basin (Belgium) and thirdly we evaluate the application of magnetic susceptibility (MS) as a tool for long-term trends, correlation, and palaeoenvironmental reconstruction. These three goals are reached by making a detailed sedimentological, geophysical and geochemical study of the La Thure quarry. Petrographic analyses revealed eighteen microfacies which represent a carbonate platform evolution through early-late Givetian time, ranging from a homoclinal ramp to a discontinuously rimmed shelf and then a drowning shelf. These results allowed us to illustrate the fascinating environmental diversity that shaped one the largest carbonate platforms of Europe and provide an up to date model of the vertical and lateral development of the Belgian Givetian platform. Early-late Givetian La Thure sequence reveals five main depositional intervals, which could be correlated with the southern margin of the platform. These correlations allowed us to define the facies belts distribution, the major depositional changes that affected this platform and to highlight the Taghanic Event. Palaeo-redox proxies reveal a significant change in the oxygenation level, from oxygen-depleted to more oxic condition, between middle and late Givetian. This change is well-correlated with an increase in global temperature in the late Mid Devonian. Combination of MS and geochemistry demonstrates the inherent-parallel link existing between variation in MS values and proxies for siliciclastic input (such as Si, Al). Collected geochemical and MS data confirm that the La Thure can be considered as a key section for the internal shelf setting in the Rhenohercynian Ocean bordering Laurussia’s south-eastern margin. [less ▲]

Detailed reference viewed: 55 (4 ULg)
Full Text
Peer Reviewed
See detailInsights into abundant rumen ureolytic bacterial community using rumen simulation system
Jin, Di; Zhao, Shengguo; Wang, Pengpeng et al

in Frontiers in Microbiology (2016), 7(1006),

Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However ... [more ▼]

Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However, there is limited knowledge with regard to the ureolytic bacteria community in the rumen. To explore the ruminal ureolytic bacterial community, urea, or acetohydroxamic acid (AHA, an inhibitor of urea hydrolysis) were supplemented into the rumen simulation systems. The bacterial 16S rRNA genes were sequenced by Miseq high-throughput sequencing and used to reveal the ureoltyic bacteria by comparing different treatments. The results revealed that urea supplementation significantly increased the ammonia concentration, and AHA addition inhibited urea hydrolysis. Urea supplementation significantly increased the richness of bacterial community and the proportion of ureC genes. The composition of bacterial community following urea or AHA supplementation showed no significant difference compared to the groups without supplementation. The abundance of Bacillus and unclassified Succinivibrionaceae increased significantly following urea supplementation. Pseudomonas, Haemophilus, Neisseria, Streptococcus, and Actinomyces exhibited a positive response to urea supplementation and a negative response to AHA addition. Results retrieved from the NCBI protein database and publications confirmed that the representative bacteria in these genera mentioned above had urease genes or urease activities. Therefore, the rumen ureolytic bacteria were abundant in the genera of Pseudomonas, Haemophilus, Neisseria, Streptococcus, Actinomyces, Bacillus, and unclassified Succinivibrionaceae. Insights into abundant rumen ureolytic bacteria provide the regulation targets to mitigate urea hydrolysis and increase efficiency of urea nitrogen utilization in ruminants. [less ▲]

Detailed reference viewed: 14 (3 ULg)
Full Text
Peer Reviewed
See detailInsights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.
Berlemont, Renaud ULg; Delsaute, Maud ULg; Pipers, Delphine ULg et al

in ISME Journal (The) (2009), 3(9), 1070-1081

In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is ... [more ▼]

In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 degrees C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.The ISME Journal advance online publication, 21 May 2009; doi:10.1038/ismej.2009.48. [less ▲]

Detailed reference viewed: 113 (38 ULg)
Full Text
Peer Reviewed
See detailInsights into behavioral vulnerability to differential sleep pressure and circadian phase from a functional ADA polymorphism.
Reichert, Carolin F.; Maire, Micheline; Gabel, Virginie et al

in Journal of biological rhythms (2014), 29(2), 119-30

Sleep loss affects human behavior in a nonuniform manner, depending on the cognitive domain and also the circadian phase. Besides, evidence exists about stable interindividual variations in sleep loss ... [more ▼]

Sleep loss affects human behavior in a nonuniform manner, depending on the cognitive domain and also the circadian phase. Besides, evidence exists about stable interindividual variations in sleep loss-related performance impairments. Despite this evidence, only a few studies have considered both circadian phase and neurobehavioral domain when investigating trait-like vulnerability to sleep manipulation. By applying a randomized, crossover design with 2 sleep pressure conditions (40 h sleep deprivation vs. 40 h multiple naps), we investigated the influence of a human adenosine deaminase (ADA) polymorphism (rs73598374) on several behavioral measures throughout nearly 2 circadian cycles. Confirming earlier studies, we observed that under sleep deprivation the previously reported vulnerable G/A-allele carriers felt overall sleepier than G/G-allele carriers. As expected, this difference was no longer present when sleep pressure was reduced by the application of multiple naps. Concomitantly, well-being was worse in the G/A genotype under sleep loss when compared to the nap protocol, and n-back working memory performance appeared to be specifically susceptible to sleep-wake manipulation in this genotype. When considering psychomotor vigilance performance, however, a higher sensitivity to sleep-wake manipulation was detected in homozygous participants, but specifically at the end of the night and only for optimal task performance. Although these data are based on a small sample size and hence require replication (12 G/A- and 12 G/G-allele carriers), they confirm the assumption that interindividual differences regarding the effect of sleep manipulation highly depend on the cognitive task and circadian phase, and thus emphasize the necessity of a multimethodological approach. Moreover, they indicate that napping might be suitable to counteract endogenously heightened sleep pressure depending on the neurobehavioral domain. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailInsights into fast-track colon surgery: a plea for a tailored program.
Pellegrino, L.; Lois, Fernande ULg; Remue, C. et al

in Surgical Endoscopy (2013), 27(4), 1178-85

BACKGROUND: This retrospective study compared the fast-track colon surgery program to conventional perioperative care and assessed factors that influence postoperative length of stay. DESIGN: This ... [more ▼]

BACKGROUND: This retrospective study compared the fast-track colon surgery program to conventional perioperative care and assessed factors that influence postoperative length of stay. DESIGN: This retrospective study included 124 fast-track and 119 conventional care colon surgical patients. Exclusion criteria were primary rectal disease, stoma, American Society of Anesthesiologists score IV, and Association Francaise de Chirurgie index 3 or 4. Laparoscopy was the preferred approach. Variables influencing length of stay were analyzed by multivariate linear and logistic regression. RESULTS: Overall mortality and complication rates were not significantly different between groups (fast-track vs. controls 0 vs. 0.8 %, 30.6 vs. 38.6 % respectively). As expected, median length of stay was significantly reduced in fast-track patients (3 vs. 6 days, p < 0.001), but emergency readmission rate was higher (16.9 vs. 7.6 %, p = 0.026), although rehospitalization rates were similar (8 vs. 4.2 %, not significant). Independent risk factors of increased length of stay were identified as age >69 years (p = 0.001), laparotomy (p = 0.011), and conventional perioperative care (p < 0.001). CONCLUSIONS: The introduction of a fast-track program reduced postoperative length of stay without increasing complication rate. This study proposes a modulation of the program according to patient age and surgical approach. [less ▲]

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailInsights into hallucinations in schizophrenia: novel treatment approaches
Aleman, André; Laroi, Frank ULg

in Expert Review of Neurotherapeutics (2011), 11

Detailed reference viewed: 120 (6 ULg)
Full Text
Peer Reviewed
See detailInsights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors
Tillemans, Vinciane ULg; Leponce, Isabelle ULg; Rausin, Glwadys ULg et al

in Plant Cell (2006), 18(11), 3218-3234

Serine/arginine-rich (SR) proteins are splicing regulators that share a modular structure consisting of one or two N-terminal RNA recognition motif domains and a C-terminal RS-rich domain. We investigated ... [more ▼]

Serine/arginine-rich (SR) proteins are splicing regulators that share a modular structure consisting of one or two N-terminal RNA recognition motif domains and a C-terminal RS-rich domain. We investigated the dynamic localization of the Arabidopsis thaliana SR protein RSZp22, which, as we showed previously, distributes in predominant speckle-like structures and in the nucleolus. To determine the role of RSZp22 diverse domains in its nucleolar distribution, we investigated the subnuclear localization of domain-deleted mutant proteins. Our results suggest that the nucleolar localization of RSZp22 does not depend on a single targeting signal but likely involves different domains/motifs. Photobleaching experiments demonstrated the unrestricted dynamics of RSZp22 between nuclear compartments. Selective inhibitor experiments of ongoing cellular phosphorylation influenced the rates of exchange of RSZp22 between the different nuclear territories, indicating that SR protein mobility is dependent on the phosphorylation state of the cell. Furthermore, based on a leptomycin B- and fluorescence loss in photobleaching-based sensitive assay, we suggest that RSZp22 is a nucleocytoplasmic shuttling protein. Finally, with electron microscopy, we confirmed that RSp31, a plant-specific SR protein, is dynamically distributed in nucleolar cap-like structures upon phosphorylation inhibition. Our findings emphasize the high mobility of Arabidopsis SR splicing factors and provide insights into the dynamic relationships between the different nuclear compartments. [less ▲]

Detailed reference viewed: 45 (11 ULg)
Full Text
Peer Reviewed
See detailInsights into oxygen transport and net community production in sea ice from oxygen, nitrogen and argon concentrations
Zhou, Jiayun ULg; Delille, Bruno ULg; Brabant, F. et al

in Biogeosciences (2014), 11

We present the evolution of O2 standing stocks, saturation levels and concentrations in landfast sea ice, collected in Barrow (Alaska), from February to June 2009. The comparison of the standing stocks ... [more ▼]

We present the evolution of O2 standing stocks, saturation levels and concentrations in landfast sea ice, collected in Barrow (Alaska), from February to June 2009. The comparison of the standing stocks and saturation levels of O2 against those of N2 and Ar suggests that the dynamic of O2 in sea ice strongly depends on physical processes (gas incorporation and subsequent transport). We then discuss on the use of O2 / Ar and O2 / N2 to correct for the physical contribution and to determine the biological contribution (NCP) to O2 supersaturations. We conclude that O2 / Ar suits better than O2 / N2, because O2 / N2 is more sensitive due to the relative abundance of O2, N2 and Ar, and less biased when gas bubble formation and gas diffusion are maximized. We further estimate the NCP in the impermeable layers during ice growth and in the permeable layers during ice decay. Our results indicate that NCP contributed to a~release of carbon to the atmosphere in the upper ice layers, but to an uptake of carbon at sea ice bottom. Overall, seawater (rather than the atmosphere) may be the main supplier of carbon for sea ice microorganisms. [less ▲]

Detailed reference viewed: 41 (7 ULg)
See detailInsights into the ancient methods of preparation of Prussian blue pigments by high-resolution powder diffraction and PDF analysis
Samain, Louise ULg; Martinetto, Pauline; Bordet, Pierre et al

Conference (2011, September 09)

Detailed reference viewed: 65 (3 ULg)