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See detailIsolated systolic and diastolic dippers, an intermediate subgroup between dippers and non-dippers
Moonen, martial; Saint-Remy, Annie ULg; Krzesinski, Jean-Marie ULg et al

Conference (1997, October 04)

Detailed reference viewed: 16 (1 ULg)
See detailIsolated transient UV auroral structures at Jupiter: possible signatures of magnetospheric injections 
Dumont, Maïté ULg; Grodent, Denis ULg; Radioti, Aikaterini ULg et al

Conference (2013, July 11)

We investigate transient ultraviolet auroral features located equatorward of the main emission (130 features) based on Hubble Space Telescope (HST) observations of the northern and southern Jovian ... [more ▼]

We investigate transient ultraviolet auroral features located equatorward of the main emission (130 features) based on Hubble Space Telescope (HST) observations of the northern and southern Jovian hemispheres (2000-2007). Several properties of the auroral emissions are analyzed, such as their position in auroral region, power and brightness. Additionally, we magnetically map the auroral structures to the equatorial plane using VIPAL model and we compare their observed properties with those of magnetospheric injections observed by Galileo. We suggest that these transient auroral structures could be related to magnetospheric injections. The mapped radial position and system III longitude of the observed auroral features are in good agreement with those of the injections observed in the equatorial plane by Galileo. Based on power and brightness of the auroral features, we discuss the mechanisms involved in the ionosphere-magnetosphere coupling injections. This comparative study demonstrates that the structures under study are related to magnetospheric injections and sheds light to the mechanism involved in the magnetosphere-ionosphere dynamics. [less ▲]

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See detailIsolating auroral FUV emission lines using compact, broadband instrumentation
Molyneux, P.M.; Bannister, N.P.; Bunce, E.J. et al

in Planetary and Space Science (2014), 103

Images of auroral emissions at far ultraviolet (FUV, 122–200 nm) wavelengths are useful tools with which to study magnetospheric-ionospheric coupling, as the scattered sunlight background in this region ... [more ▼]

Images of auroral emissions at far ultraviolet (FUV, 122–200 nm) wavelengths are useful tools with which to study magnetospheric-ionospheric coupling, as the scattered sunlight background in this region is low, allowing both dayside and nightside auroras to be imaged simultaneously. The ratio of intensities between certain FUV emission lines or regions can be used to characterise the precipitating particles responsible for auroral emissions, and hence is a useful diagnostic of magnetospheric dynamics. Here, we describe how the addition of simple transmission filters to a compact broadband imager design allows far ultraviolet emission ratios to be deduced while also providing large-scale instantaneous images of the aurora. The low mass and volume of such an instrument would make it well-suited for both small satellite Earth-orbiting missions and larger outer planet missions from which it could be used to characterise the tenuous atmospheres observed at several moons, as well as studying the auroral emissions of the gas giants. We present a study to investigate the accuracy of a technique to allow emission line ratio retrieval, as applied to the OI 130.4 nm and 135.6 nm emissions at Ganymede. The ratio of these emissions provides information about the atmospheric composition, specifically the relative abundances of O and O2. Using modelled FUV spectra representative of Ganymede's atmosphere, based on observations by the Hubble Space Telescope (HST) Space Telescope Imaging Spectrograph (STIS), we find that the accuracy of the retrieved ratios is a function of the magnitude of the ratio, with the best measurements corresponding to a ratio of ∼1.3 . [less ▲]

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See detailIsolating the 130.4 nm and 135.6 nm emissions in Ganymede’s aurora using broadband optics
Molyneux, Philippa M; Grodent, Denis ULg; Bunce, Emma J et al

Conference (2012, September 27)

We discuss a technique for isolating the two main Far Ultraviolet emission lines in Ganymede’s aurora by adding flight proven transmission filters to a broad- band, wide-field imager design. We find that ... [more ▼]

We discuss a technique for isolating the two main Far Ultraviolet emission lines in Ganymede’s aurora by adding flight proven transmission filters to a broad- band, wide-field imager design. We find that the ra- tio of OI emissions at 135.6 nm and 130.4 nm can be recovered if the transmission of the filters and other optical elements are well known. This ratio allows constraints to be placed on the relative abundances of O atoms and O2 molecules within Ganymede’s at- mosphere, leading to more accurate models of atmo- spheric composition. [less ▲]

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See detailIsolation ad Characterization of osteocalcin in horses
Carstanjen, B; Wattiez, R; Amory, Hélène ULg et al

in Proceedings of the ECVS meeting 2002 (Resident Forum) (2002)

Detailed reference viewed: 4 (0 ULg)
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See detailIsolation and analysis of a putative FLC orthologue in Sinapis alba L.
D'Aloia, Maria ULg; Melzer, Siegbert; Périlleux, Claire ULg

in Acta Physiologiae Plantarum (2004), 26(3), 23-24

In many plant species, flowering is promoted by a long exposure to low temperature, a process known as vernalization. Some plants even have an absolute requirement for winter cold before being able to ... [more ▼]

In many plant species, flowering is promoted by a long exposure to low temperature, a process known as vernalization. Some plants even have an absolute requirement for winter cold before being able to flower the next summer, hence behave as biennials or winter crops. A recent breakthrough in the understanding of the molecular bases of vernalization has been the cloning of the FLOWERING LOCUS C (FLC) gene in Arabidopsis thaliana (Michaels S.D. & Amasino R.M., 1999). FLC encodes a repressor of flowering and is downregulated by vernalization. So vernalization relieves the inhibitory role that FLC plays on downstream genes involved in the floral transition of the shoot apical meristem (SAM). Although vernalization has been shown, by physiological studies, to be sensed by the SAM, molecular evidences are missing. Such analyses are impaired in Arabidopsis by the small size of the plant and the rosette growth habit. We therefore cloned a FLC homologue in a caulescent relative Brassicaceae: mustard (Sinapis alba L.). We identified two clones by screening a cDNA library made from leaf mRNA. One of them, SaFLC1, was used for in situ hybridizations on SAM sections. Preliminary results are shown. Because of its agronomical importance, we have also tried to manipulate the vernalization process in crops. By using a transgenic approach, we have attempted to bypass the strict vernalization requirement of winter colza (Brassica napus L.). We have over-expressed SaMADS A, a gene which is repressed by FLC in wild type, and observed that the transgenics did not require vernalization any more. [less ▲]

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See detailIsolation and biomass production of a Saccharomyces cerevisiae strain binding copper and zinc ions
Stroobants, Aurore ULg; Delroisse, Jean-Marc; Delvigne, Frank ULg et al

in Applied Biochemistry and Biotechnology (2009), 157(1),

Detailed reference viewed: 83 (24 ULg)
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See detailIsolation and Characterisation of a Ruminant Alphaherpesvirus Closely Related to Bovine Herpesvirus 1 in a Free-Ranging Red Deer
Thiry, Julien ULg; Widen, F.; Grégoire, Fabien ULg et al

in BMC Veterinary Research (2007), 3

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine ... [more ▼]

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid herpesviruses 1 (CvHV-1) and 2 (CvHV-2) and elk herpesvirus 1 (ElkHV-1). Considering the serological relationship between these ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV-1 related virus infection in wild and domestic ruminant species. In this way, a recent investigation has indicated, in Belgium, a high increase in the serological prevalence of BoHV-1 related virus infection in free-ranging red deer population. In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population. RESULTS: The current study reports the first isolation in a free-ranging red deer of a BoHV-1 closely related virus. The isolate was antigenically, genomically and genetically characterised by comparison with several ruminant alphaherpesvirus. Immunofluorescence assays revealed the isolate was antigenically distinct from bovine and caprine alphaherpesviruses. Similarly, BamHI and BstEII restriction analyses demonstrated the genomic difference between the isolate and the other ruminant alphaherpesviruses. Next, the sequencing of selected parts of UL27 and US8 genes showed a high degree of homologies between each BoHV-1 related ruminant alphaherpesvirus and the isolate. Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1. CONCLUSION: The first isolation of a virus closely related to BoHV-1 in a free-ranging red deer is reported. Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe. Anlier strain show consistent differences with the virus isolated from Scottish farmed red deer. All together, these results improve our understanding of ruminant alphaherpesviruses. [less ▲]

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See detailIsolation and characterisation of new alien monosomic addition lines of Gossypium australe F. Muell. on G. hirsutum L.
Mergeai, Guy ULg; Sarr, D.; Ahoton, L. et al

in Proceedings of the 2004 International cotton initiative workshop (2004)

Detailed reference viewed: 22 (5 ULg)
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See detailIsolation and characterisation of new spore-forming lactic acid bacteria with prospects of use in food fermentations and probiotic preparations
Bayane, Ali; Diawara, B.; Dubois Dauphin, Robin ULg et al

in African Journal of Microbiology Research [=AJMR] (2010), 4(11), 1016-1025

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See detailIsolation and characterisation of seven alien monosomic addition lines of Gossypium australe F. Muell. on G. hirsutum L.
Ahoton, L.; Lacape, J. M.; D'Hont, A. et al

in World Cotton Research Conference - 3. Cotton production for the new Millenium (2004)

Detailed reference viewed: 56 (8 ULg)
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See detailIsolation And Characterization Of A 2.3-Kilobase-Pair Cdna Fragment Encoding The Binding Domain Of The Bovine Leukemia-Virus Cell-Receptor
Ban, J.; Portetelle, Daniel ULg; Altaner, C. et al

in Journal of Virology (1993), 67(2),

Detailed reference viewed: 10 (1 ULg)
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See detailIsolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bulalis) in Argentina
Maidana, S.; Lomonaco, M.; Odeon, A. et al

in BMC Veterinary Research (2012), 8

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See detailIsolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation
El Amiri, B.; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Reproduction Nutrition Development (2004), 44(3, May-Jun), 169-181

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the ... [more ▼]

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the present study was to isolate as many PAG molecules as possible from placentae collected between day 60 and day 100 of gestation and to characterize their amino-terminal amino-acid sequences. Three heterologous radioimmunoassays were used to monitor PAG immunoreactivity throughout the isolation procedures. Sequential use of DEAE-cellulose, gel filtration, and CM ceramic chromatographies led to the isolation of several fractions rich in PAG immunoreactivity. The fractions with a large amount of proteins were also purified by chromatofocusing. The analysis of immunoreactive fractions by SDS-PAGE, Western blotting and two-dimensional electrophoresis followed by amino-terminal microsequencing on PVDF membranes allowed to identify eight different ovPAG with apparent molecular masses ranging from 55 to 66 kDa and isoelectric points from 4.0 to 6.8. The N-terminal sequences were determined and their comparison to those previously identified revealed that four of them are identical to those encoded by previously known cDNA, while the additional four sequences appear to be novel since they have not yet been described. [less ▲]

Detailed reference viewed: 32 (1 ULg)
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See detailThe isolation and characterization of equine Osteocalcin
Carstanjen, B; Wattiez, R; Amory, Hélène ULg et al

in Proceedings of the 1st meeting of the International Bone and Mineral Society (IBMS) (2001)

Detailed reference viewed: 28 (0 ULg)
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See detailIsolation and characterization of mutants deficient in four steps of the phylloquinone biosynthesis pathway in Chlamydomonas reinhardtii.
Emonds-Alt, Barbara ULg; Remacle, Claire ULg; Cardol, Pierre ULg

Poster (2016, April 26)

In photosystem I (PSI), phylloquinone participates to electron transfer as secondary electron acceptor (A1). The phylloquinone biosynthesis pathway, previously characterized by reverse genetic in ... [more ▼]

In photosystem I (PSI), phylloquinone participates to electron transfer as secondary electron acceptor (A1). The phylloquinone biosynthesis pathway, previously characterized by reverse genetic in Synechocystis sp. PCC 6803, involves 8 enzymatic steps from chorismate [1]. In the green alga Chlamydomonas reinhardtii, characterization of phylloquinone biosynthesis was still partial and only one mutant deficient for MEND was characterized [2]. In the present work, we found MENA-H homologs in C. reinhardtii genomic database. In particular, MENF, MEND, MENC, and MENH catalytic domains are present in a single ORF (named PHYLLO by similarity to gene organisation in Arabidopsis). We then took advantage of the fact that a double reduction of plastoquinone (PQ) in PQH2 occurs in anoxia into the A1 site in the mend mutant, interrupting photosynthetic electron transfer [3], to isolate new phylloquinone-deficient strains. UPLC-MS analysis confirmed the absence of phylloquinone in four news mutants impaired in MENA, MENB, MENC (PHYLLO) and MENE. Despite this loss, men mutants are still able to grow in low light but are high light-sensitive. In low light, the level of active PSII in men mutants is identical to that of the wild-type, but the level of active PSI is reduced by 30-40% as assayed by spectroscopic measurements. This decrease is more pronounced when cells are exposed to high light intensities during 4 hours. The level of active PSI is ~ 10% of wild-type cells and the electron photosynthetic transfer is reduced accordingly. Reorganization of the photosynthetic apparatus following lack of phylloquinone in men mutants is discussed. [less ▲]

Detailed reference viewed: 27 (11 ULg)
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See detailIsolation and characterization of mutants deficient in four steps of the phylloquinone biosynthesis pathway in Chlamydomonas reinhardtii.
Emonds-Alt, Barbara ULg; Remacle, Claire ULg; Cardol, Pierre ULg

Poster (2016, April 26)

In photosystem I (PSI), phylloquinone participates to electron transfer as secondary electron acceptor (A1). The phylloquinone biosynthesis pathway, previously characterized by reverse genetic in ... [more ▼]

In photosystem I (PSI), phylloquinone participates to electron transfer as secondary electron acceptor (A1). The phylloquinone biosynthesis pathway, previously characterized by reverse genetic in Synechocystis sp. PCC 6803, involves 8 enzymatic steps from chorismate [1]. In the green alga Chlamydomonas reinhardtii, characterization of phylloquinone biosynthesis was still partial and only one mutant deficient for MEND was characterized [2]. In the present work, we found MENA-H homologs in C. reinhardtii genomic database. In particular, MENF, MEND, MENC, and MENH catalytic domains are present in a single ORF (named PHYLLO by similarity to gene organisation in Arabidopsis). We then took advantage of the fact that a double reduction of plastoquinone (PQ) in PQH2 occurs in anoxia into the A1 site in the mend mutant, interrupting photosynthetic electron transfer [3], to isolate new phylloquinone-deficient strains. UPLC-MS analysis confirmed the absence of phylloquinone in four news mutants impaired in MENA, MENB, MENC (PHYLLO) and MENE. Despite this loss, men mutants are still able to grow in low light but are high light-sensitive. In low light, the level of active PSII in men mutants is identical to that of the wild-type, but the level of active PSI is reduced by 30-40% as assayed by spectroscopic measurements. This decrease is more pronounced when cells are exposed to high light intensities during 4 hours. The level of active PSI is ~ 10% of wild-type cells and the electron photosynthetic transfer is reduced accordingly. Reorganization of the photosynthetic apparatus following lack of phylloquinone in men mutants is discussed. [less ▲]

Detailed reference viewed: 41 (12 ULg)