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See detailMorphogenesis of bovine leukemia virus (BLV).
Dekegel, D.; Mammerickx, Marc; Burny, Arsène et al

in Burny, Arsène (Ed.) Bovine leukosis: various methods of molecular virology (1977)

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See detailMorphological adaptations of Pearlfish (Carapidae) to their various habitats
Parmentier, Eric ULg; Vandewalle, Pierre ULg

in Kapoor, B. G.; Val, Aldaberto Luis (Eds.) Fish adaptations (2003)

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See detailMorphological alterations in oxidative muscles and mitochondrial structure associated with equine atypical myopathy
Cassart, Dominique ULg; Baise, Etienne ULg; Cherel, Yann et al

in Equine Veterinary Journal (2007), 39(1), 26-32

REASONS FOR PERFORMING STUDY: There is a lack of well documented studies about muscular lesions in equine atypical myopathy (EAM). <br /> <br />OBJECTIVES: To characterise morphopathological changes of ... [more ▼]

REASONS FOR PERFORMING STUDY: There is a lack of well documented studies about muscular lesions in equine atypical myopathy (EAM). <br /> <br />OBJECTIVES: To characterise morphopathological changes of striated muscles and myocardium, to progress understanding of this disease. <br /> <br />METHODS: Thirty-two horses age 0.5-7 years kept on pasture were referred for a sudden ataxia/myoglobinuria syndrome. Clinical examination (stiffness, muscle pain, muscle fasciculations, abnormal gait, recumbency, myoglobinuria, tachycardia, sweating) and plasma CPK, LDH and AST levels were consistent with extensive myonecrosis and, together with anamnestic data, with so-called 'equine atypical myopathy' (EAM), a disease of unknown aetiology reported since 1939. Macroscopic and microscopic (histology, histoenzymology, ultrastructure) lesions were evaluated. <br /> <br />RESULTS: Necropsic examination revealed large areas of muscle necrosis, the extent and severity of which varied between cases and muscles, but which were clearly more constant and severe in respiratory and postural muscles and in the myocardium. Histology highlighted a multifocal and monophasic process compatible with Zenker degeneration/necrosis that mostly and segmentally affected type 1 fibres. Histochemical evaluation revealed a weak and disorganised pattern of NADH tetrazolium reductase staining, the absence of calcium salts precipitates and a dramatic accumulation of lipid droplets. Ultrastructural examination often revealed fibres of which the sole modifications were altered mitochondria and sarcoplasmic lipidosis. <br /> <br />CONCLUSIONS: Taken together, the data suggest that a primary alteration of mitochondria should be considered, although secondary mitochondrial abnormalities have yet to be ruled out. <br /> <br />POTENTIAL RELEVANCE: The morphological features gathered here reveal that EAM shares most of the characteristics of toxic myopathies. [less ▲]

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See detailMorphological alterations of blood platelets induced by Platelet Activating Factor (PAF) and partial inhibition by Ketoprofen in calves
Bastos da Silva, Miriam; Doizé, Cécile ULg; David, Jean-Louis ULg et al

in Veterinary Research (1997), 28(5 Sep-Oct), 489-502

The influence of platelet activating factor (PAF) was investigated in vivo on the ultrastructure of bovine platelets, and on the platelet count. The effect of an intravenous administration of ketoprofen ... [more ▼]

The influence of platelet activating factor (PAF) was investigated in vivo on the ultrastructure of bovine platelets, and on the platelet count. The effect of an intravenous administration of ketoprofen (a non-steroidal anti-inflammatory drug) pretreatment followed by PAF infusion was also observed in a group of six healthy male Friesian calves. PAF infusion alone caused a moderate thrombocytopenia, which peaked one minute post challenge and returned to levels not significantly different from control after 30 min. Electron microscopy revealed that after PAF infusion, platelets lost their lentiform shape and became irregular, with many pseudopods. Their microtubules became impossible to distinguish. The numbers of alpha granules and dense bodies were significantly decreased. Glycogen particles became rare or even disappeared. Giant platelets occasionally appeared. The Golgi apparatus was more often visible and the number of mitochondria was significantly increased. Ketoprofen pretreatment lowered PAF-induced thrombocytopenia and decrease in the number of dense bodies. Under these conditions, the Golgi apparatus was rarely visible and giant platelets were not observed. These results showed that the morphological ultrastructure of blood platelets in bovines were modified following PAF infusion and that ketoprofen pretreatment before PAF infusion provided partial protection, limiting the extent of the morphological alterations and maintaining a normal platelet count [less ▲]

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See detailMorphological alterations of zooxanthellae in bleached cnidarian hosts
Ladrière, Ophélie ULg; Compère, Philippe ULg; Decloux, Nicole ULg et al

in Cahiers de Biologie Marine (2008), 49(3), 215-227

Studying the morphological changes of zooxanthellae in the host gastroderm is essential to understand the mechanisms of bleaching. Transmission electron microscopy was used to observe samples from four ... [more ▼]

Studying the morphological changes of zooxanthellae in the host gastroderm is essential to understand the mechanisms of bleaching. Transmission electron microscopy was used to observe samples from four coral species-three collected from a barrier reef in Madagascar (Acropora digitifera (Dana, 1846), Echinopora hirsutissima Milne-Edwards & Haime, 1849 and Porites (Synaraea) rus Forskal, 1775)) and one cut from an aquarium-grown coral (Pocillopora damicornis (Linnaeus, 1758)-and from the hermatypic (zooxanthellae-containing) sea anemone Aiptasia pulchella (Carlgren, 1943). Zooxanthellae from bleached animals showed different stages of degradation or disorganization. Some were free, detached from the host gastroderm, associated or not with host-cell remains. Others were vacuolated, with abundant reserve material globules and angular holes probably created by the loss of crystalline material during cutting. Experimentally heat-shocked P. damicornis harboured, moreover, a greater number of dividing algae. Bleached individuals were found to vary as regards their response to stress, and zooxanthellae expelled from heat-shocked anemones showed a greater mitotic index and a higher survival rate than algae extracted or naturally externalized from healthy individuals. We propose a combination of morphological criteria for use in diagnosing the health state of algae-cnidarian symbiosis, so vulnerable in the case of bleaching. [less ▲]

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See detailMorphological analysis of overproduction of hair cells and Deiters'cells in the E19 rat organ of Corti
Thiry, Marc ULg; Malgrange, B; Lefebvre, P

in Acta Oto-Rhino-Laryngologica Belgica (2002), 56

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See detailMorphological Analysis of Slip-Cast Emulsion-Templated Alumina Foams by Microfocus Computer Tomography
Neirinck, Bram; Kerckhofs, Greet ULg; Wevers, Martine et al

in JOURNAL OF THE AMERICAN CERAMIC SOCIETY (2010), 93(11), 3921-3928

Bulk alumina foams were generated by slip casting of suspensions combined with particle-stabilized emulsion droplets as pore templates. Because of the volatile nature of the template, the green foams ... [more ▼]

Bulk alumina foams were generated by slip casting of suspensions combined with particle-stabilized emulsion droplets as pore templates. Because of the volatile nature of the template, the green foams could be consolidated by sintering without a debinding step. The porous structure of the consolidated foams was analyzed nondestructively using image analysis of microfocus computer tomography (micro-CT) data. The fraction of open and closed pores, as well as the pore sizes and pore size distributions, were calculated from the generated 3D micro-CT datasets and correlated to the applied processing parameters. The anisotropy in the produced structures was assessed and elucidated by a detailed analysis of segmented tomography data. The results indicate that the width of the pore size distribution and the average macropore size, ranging from 225 to 136 mu m, depend on the slip rheology. Any anisotropy caused in the structure was linked to the template preparation but is only present in a narrow zone near the bottom of the foams. Although the macroporosity (>= 20 mu m) was only 24%-31%, tomographic analysis showed that over 90% of these macropores were interconnected through >= 20-mu m-diameter windows. [less ▲]

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See detailMorphological and allozyme variation in a collection of Cucumeropsis mannii Naudin (Cucurbitaceae) from Côte d'Ivoire
Koffi, Kouamé K.; Gbotto, Ahou A.; Malice, Marie ULg et al

in Biochemical Systematics & Ecology (2008), (36), 777-789

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See detailMorphological and allozyme variation in a collection of Lagenaria siceraria (Molina) Standl. from Côte d'Ivoire
Koffi, K. K.; Baudoin, Jean-Pierre ULg; Malice, Marie et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13(2), 257-281

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See detailMorphological and ecological complexity in early eukaryotic ecosystems
Javaux, Emmanuelle ULg; Knoll, A. H.; Walter, M. R.

in Nature (2001), 412(6842), 66-69

Molecular phylogeny and biogeochemistry indicate that eukaryotes differentiated early in Earth history. Sequence comparisons of small-subunit ribosomal RNA genes suggest a deep evolutionary divergence of ... [more ▼]

Molecular phylogeny and biogeochemistry indicate that eukaryotes differentiated early in Earth history. Sequence comparisons of small-subunit ribosomal RNA genes suggest a deep evolutionary divergence of Eukarya and Archaea(1); C-27-C-29 steranes (derived from sterols synthesized by eukaryotes) and strong depletion of C-13 (a biogeochemical signature of methanogenic Archaea) in 2,700 Myr old kerogens independently place a minimum age on this split(2,3). Steranes, large spheroidal microfossils, and rare macrofossils of possible eukaryotic origin occur in Palaeoproterozoic rocks(4-6). Until now, however, evidence for morphological and taxonomic diversification within the domain has generally been restricted to very late Mesoproterozoic and Neoproterozoic successions(7). Here we show that the cytoskeletal and ecological prerequisites for eukaryotic diversification were already established in eukaryotic microorganisms fossilized nearly 1,500 Myr ago in shales of the early Mesoproterozoic Roper Group in northern Australia. [less ▲]

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See detailMorphological and functional characterization of Carnobacterium maltaromaticum isolated from vacuum-packed beef with long shelf life
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Ndedi Ekolo, François et al

Poster (2014, August)

The aim of this study was to perform a morphological and functional characterization of a Carnobacterium maltaromaticum strain with a potential bioprotective effect isolated from vacuum packaged long ... [more ▼]

The aim of this study was to perform a morphological and functional characterization of a Carnobacterium maltaromaticum strain with a potential bioprotective effect isolated from vacuum packaged long shelf life beef. The morphological, biochemical and enzymatic profiles, the influence of different temperatures and atmospheres, and the microbial stability of fresh beef inoculated with the C. maltaromaticum strain were evaluated. The isolated C. maltaromaticum strain presented similar morphological, biochemical and enzymatic profiles as those of two reference strains (LMG 11393 and LMG 22902). The growth of C. maltaromaticum was slower in an atmosphere containing O2 and CO2. Vacuum packing is therefore suitable for this bacterium. An antimicrobial effect against Enterobacteriaceae was highlighted on inoculated fresh meat stored under N2. The functional characterization of this isolate will be further pursued by a genotypic characterization to better understand its potential bioprotective effect. [less ▲]

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See detailMORPHOLOGICAL AND GENETIC CRITERIA IN THE TAXONOMY OF CYANOPHYTA CYANOBACTERIA
Wilmotte, Annick ULg; Golubic, Stjepko

in Archiv für Hydrobiologie (1991), 92

Molecular techniques have introduced new approaches to the phylogeny and taxonomy of Cyanophyta/Cyanobacteria. One method, the sequencing of the 16S ribosomal RNA, has been applied to 29 cyanophyte ... [more ▼]

Molecular techniques have introduced new approaches to the phylogeny and taxonomy of Cyanophyta/Cyanobacteria. One method, the sequencing of the 16S ribosomal RNA, has been applied to 29 cyanophyte strains and produced interesting results (TOMIOKA & SUGIURA 1983, GIOVANNONI et al. 1988). Integration of this new, taxonomically useful information with the results of the classical morphological analyses traditionally used in the taxonomy of cyanophytes is the subject of this paper. Advantages and limitations of different approaches are discussed, and conflicts as well as consensus between morphological properties of the strains under study, and their position in the phylogenetic tree of GIOVANNONI et al. (1988) are reviewed. [less ▲]

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See detailMorphological and genetic divergences in a coral reef damselfish, Pomacentrus coelestis
Frederich, Bruno ULg; Liu, S.-Y. Vanson; Dai, C.-F.

in Evolutionary Biology (2012), 39(3), 359-370

Population differentiation is one of the main topics in evolutionary biology. Except the exploration of color variation, few studies focused on morphological divergences among populations of coral reef ... [more ▼]

Population differentiation is one of the main topics in evolutionary biology. Except the exploration of color variation, few studies focused on morphological divergences among populations of coral reef fishes. In this work, we studied morphological and genetic differentiation among populations of the damselfish, Pomacentrus coelestis, in the northwestern Pacific Ocean. The shapes of the mandible and the premaxilla were explored using geometric morphometric methods and the genetic structure was investigated using microsattelites. Various tests revealed significant shape variation among most P. coelestis populations for both skeletal units. Morphological variation of the mandible accompanies a genetic break between populations of mainland Japan and Okinawa-Taiwan. However, Mantel and Procrustes tests revealed no congruence between morphological and genetic structures. We illustrate that phenotypic plasticity and adaptive divergence are potential evolutionary mechanisms underlying shape difference among P. coelestis populations. An ecomorphological approach suggests that various diet could be related to shape variation of oral jaws. [less ▲]

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See detailMorphological and histological studies of sheep’s brain
Salouci, Moustafa ULg; Engelen, Virginie; Jacqmot, Olivier ULg et al

Poster (2011, December)

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the ... [more ▼]

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and cerebellum cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus. [less ▲]

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See detailMorphological and histological studies of sheep’s brain
Salouci, Moustafa ULg; Engelen, Virginie; Jacqmot, Olivier ULg et al

Poster (2010)

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the ... [more ▼]

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and cerebellum cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus. [less ▲]

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See detailMorphological and hormonal characterisation of strawberry vitroplants raised through axillary or stipular adventitious shooting
Jemmali, Ahmed; Elloumi, Nedra; Kevers, Claire ULg et al

in Plant Growth Regulation (2002), 38(3), 273-278

Adventitious stipular bud formation occurred in vitro in many strawberry cultivars during the proliferation phase on medium containing Knop macronutrients, MS micronutrients, vitamins, aminoacids, 2.22 ... [more ▼]

Adventitious stipular bud formation occurred in vitro in many strawberry cultivars during the proliferation phase on medium containing Knop macronutrients, MS micronutrients, vitamins, aminoacids, 2.22 muM BAP, 2.46 muM IBA and 0.29 muM GA(3). As described previously for cultivar Gorella, cultivar Elsanta also showed adventitious stipular buds developing on the abaxial median zone between the stipule tips. To compare the shoots produced from both types of buds, clonal propagation was initiated from stipular buds and from axillary buds on the above mentioned medium. Stipular buds were separated from the meristem-tip initiated plantlet and cultivated in the presence of a lower BAP concentration (1.33 muM) to prevent further stipular bud formation. During proliferation cycles, stipular originated propagules were very easily distinguished by their specific leaf phenotype and light green colour in comparison to plantlets cloned for an axillary bud. Their multiplication rate and cytokinin content were also higher than for axillary buds. No significant difference was observed in auxin content. [less ▲]

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See detailMorphological and mechanical characterisation of bone scaffolds
Kerckhofs, Greet ULg; Wevers, Martine; Schrooten, Jan

Poster (2005)

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