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See detailIsolation and identification of potential antimalarial compounds from endemic plants of Reunion Island
Bordignon, Annélise ULg; Cieckiewicz, Ewa ULg; Jansen, Olivia ULg et al

Poster (2015, July 16)

Malaria is known as the most important parasitic disease around the world with 584 000 malaria deaths worldwide in 2013 [1]. Due to the problem of increased parasite resistance, natural products from ... [more ▼]

Malaria is known as the most important parasitic disease around the world with 584 000 malaria deaths worldwide in 2013 [1]. Due to the problem of increased parasite resistance, natural products from endemic plants of Reunion Island, hot spot of promising biodiversity, could represent an important source of new antimalarial drugs. The aim of this thesis research focuses on the evaluation of potential antiplasmodial activity of medicinal plants from Reunion Island. A global screening of plants extracts from Reunion Island was performed on Plasmodium falciparum 3D7 chloroquine-sensitive strain revealed by colorimetric method as described in previous reports [2]. Monimia rotundifolia was then selected due to its promising in vitro activity against Plasmodium. Bioguided fractionation was realized using Prep HPLC techniques and led to the isolation of aporphine-type alkaloids from Monimia rotundifolia leaves dichloromethane extract. Further investigations are in process to confirm the antiplasmodial activities of these alkaloids and to determine their structures. References: [1] WHO, World Malaria report 2014. [2] Jansen O. et al., Evaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties. J Ethnopharmacol 2010, 130:143-150. [less ▲]

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See detailIsolation and Identification of Steroid and Flavonoid Glycosides from the Flowers of Allium gramineum
Mskhiladze, Lasha; Chincharadze, David; St-Gelais, Alexis et al

in International Journal of Pharmaceutical Sciences and Drug Research (2016), 8(4), 240-243

The isolation and identification of β-sitosterol 3-O-β-glucopyranoside, quercetin 3-O-β-glucopyranoside, isorhamnetin 3,4'-di-O-β-glucopyranosideand isorhamnetin 3,7-di-O-β-glucopyranosidefrom the flowers ... [more ▼]

The isolation and identification of β-sitosterol 3-O-β-glucopyranoside, quercetin 3-O-β-glucopyranoside, isorhamnetin 3,4'-di-O-β-glucopyranosideand isorhamnetin 3,7-di-O-β-glucopyranosidefrom the flowers of Allium gramineumthat growsin Georgia.The structures of isolated compounds havebeen determined by 1H and 13C nuclear magnetic resonance (NMR) Isolation and Identification of Steroid and Flavonoid Glycosides from the Flowers of Allium gramineum. Available from: https://www.researchgate.net/publication/305851744_Isolation_and_Identification_of_Steroid_and_Flavonoid_Glycosides_from_the_Flowers_of_Allium_gramineum [accessed Aug 5, 2016]. [less ▲]

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See detailIsolation and identification of two new fungal strains for xylanase production
Bakri, Y.; Masson, M.; Thonart, Philippe ULg

in Applied Biochemistry and Biotechnology (2010), 162(6), 1626-1634

Fungi are well known for their ability to excrete enzymes into the environment. The aim of this work was to evaluate xylanase production by fungi isolated from soil. One hundred and thirty-six fungal ... [more ▼]

Fungi are well known for their ability to excrete enzymes into the environment. The aim of this work was to evaluate xylanase production by fungi isolated from soil. One hundred and thirty-six fungal isolates were screened for xylanase production. Two xylanase producing isolates, FSS117 and FSS129, were identified on the basis of analyses of 5,8S gene sequencing. The closest phylogenetic neighbors according to 5,8S gene sequence data for the two isolates were Aspergillus tubingensis and Aspergillus terreus, respectively. When birchwood xylan or corn cob hulls was used as a substrate for 5 days under submerged culture cultivation, xylanase production from A. terreus FSS129 was 113 and 174 IU ml -1, respectively. The pH and temperature for optimum xylanase activity were 8 and 65∈°C. © 2010 Springer Science+Business Media, LLC. [less ▲]

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See detailIsolation and long-term cultivation of human tonsil follicular dendritic cells.
Tsunoda, R.; Nakayama, M.; Onozaki, K. et al

in Virchows Archiv. B : Cell pathology (1990), 59(2), 95-105

Highly purified follicular dendritic cells (FDC) were isolated from human tonsils and cultivated for up to 150 days. The cell separation method employed produced pure aggregates (FDC-clusters) composed of ... [more ▼]

Highly purified follicular dendritic cells (FDC) were isolated from human tonsils and cultivated for up to 150 days. The cell separation method employed produced pure aggregates (FDC-clusters) composed of FDC and germinal center lymphoid cells, useful for the analysis of the relationship between these two cell types and of the behavior of FDC in culture. During the first few days of culture, lymphoid cells located between FDC extensions survived better than those which were free or partly covered by FDC. After 6 days, the lymphoid population degenerated and only the FDC survived. The unique antigenic pattern of FDC (positive for HLA-DR. DRC-1, CD14b, CD21, CD23, CD35) disappeared within a few days of culture. Recombinant interferon-gamma exerted a positive effect either on retaining HLA-DR expression or on the reexpression of these antigens by FDC. HLA-ABC antigens were traced until the 10th day and desmosomal junctions until the 14th day. Subsequently, FDC presented peculiar features, including oval and rhomboid shapes, one to ten nuclei, fine amoeboid extensions, stress fibers and a radical dense zone in their cytoplasm. FDC possessed actin, tubulin and vimentin, but neither desmin nor cytokeratin. After 40 days of culture, FDC enlarged and were covered with abundant membrane extensions. Even when kept as long as 150 days in vitro. FDC did not proliferate in any of the culture conditions employed. [less ▲]

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See detailIsolation and Partial Characterization of a Pregnancy-Associated Glycoprotein Family from the Goat Placenta
Garbayo, Juana Maria; Remy, Benoit; Alabart, José Luis et al

in Biology of Reproduction (1998), 58(1), 109-115

Antigen(s) immunologically related to pregnancy-associated glycoproteins (PAGs) have previously been detected in the serum of pregnant goats. In this work, we describe a partial characterization of a ... [more ▼]

Antigen(s) immunologically related to pregnancy-associated glycoproteins (PAGs) have previously been detected in the serum of pregnant goats. In this work, we describe a partial characterization of a family of PAGs isolated from the placenta of the goat. The procedure, monitored by RIA, included extraction of proteins at neutral pH, acidic, and ammonium sulfate precipitations; and gel filtration and ion exchange chromatographies. Immunoreactivity, initially located in the acidic supernatant and in the 40-80% ammonium sulfate fractions, was equally apportioned between the 0.04 and 0.08 M NaCl DEAE fractions. After further purification of both DEAE fractions, the preparations were subjected to one- and two-dimensional electrophoresis, and individual polypeptides were analyzed by amino acid sequencing. Three PAGs, which differed in amino acid sequence and apparent molecular masses (62, 59, and 55 kDa), were detected, each containing several isoforms with different pls: caprine (c) PAG62 (pl: 5.1, 4.8), cPAG59 (pl: 6.2, 5.9, 5.6), and cPAG55 (pl: 5.3, 5.1, 4.9). These proteins had high sequence identities to each other and to PAGs purified from other species. Each had two putative N-glycosylation sites within the 27 amino terminal residues sequenced. This work demonstrates that PAGs are present in goat placenta and that multiple forms are expressed. [less ▲]

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See detailIsolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta
El Amiri, B.; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Molecular Reproduction and Development (2003), 64(2), 199-206

Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal cotyledons and characterized biochemically. The isolation procedure included acid and ammonium sulfate precipitations and anion and cation exchange chromatographies. The isolated PAGs have different NH2-terminal amino acid sequences (RGSXLTILPLRNMRDIVY, ISRVSXLTIHPLRNIMDML, and RGSNLTIHPLRNIRD) and apparent molecular masses (55, 57, and 59 kDa). Each shows several isoforms with different pl values. The three proteins share high sequence identity with each other and with other ovine, bovine, and caprine PAGs. They have not been described previously. The ovPAG-59 sequence differs from the previously identified ovPAG-4 sequence (determined by DNA cloning and sequencing) at only one position among the 15 N-terminal residues. The newly characterized ovPAGs and the procedure used to isolate them will be helpful in producing new antisera for investigating PAG secretion in pregnant ewes. [less ▲]

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See detailIsolation and partial purification of pregnancy-associated glycoproteins from sheep placenta
El Amiri, Bouchra; Remy, Benoit; Sulon, Joseph ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(1), 23-24

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See detailIsolation and pharmacological activity of phenylpropanoid esters from Marrubium vulgare
Sahpaz, S.; Garbacki, Nancy ULg; Tits, Monique ULg et al

in Journal of Ethnopharmacology (2002), 79(3), 389-392

The isolation and identification of major phenylpropanoid esters from Marrubium vulgare: (+) (E)-caffeoyl-L-malic acid 1, acteoside 2, forsythoside B 3, arenarioside 4, ballotetroside 5, as well as their ... [more ▼]

The isolation and identification of major phenylpropanoid esters from Marrubium vulgare: (+) (E)-caffeoyl-L-malic acid 1, acteoside 2, forsythoside B 3, arenarioside 4, ballotetroside 5, as well as their anti-inflammatory activity are reported for the first time, We evaluated the inhibitory effects of these five compounds on cyclooxygenase (Cox) catalysed prostaglandin biosynthesis activity. Only the glycosidic phenylpropanoid esters showed an inhibitory activity towards the Cox-2 enzyme and three of them: acteoside 2, forsythoside B 3, arenarioside 4, exhibited higher inhibitory potencies on Cox-2 than on Cox-1. These results are of interest, as Cox-2 is mainly associated with inflammation and the Cox-1 inhibition with adverse side effects often observed with non-steroidal anti-inflammatory drugs. The occurence of these phenylpropanoid esters could also explain some other pharmacological properties of M. vulgare. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. [less ▲]

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See detailIsolation and pharmacological characterization of AdTx1, a natural peptide displaying specific insurmountable antagonism of the alpha1A-adrenoceptor
Quinton, Loïc ULg; Girard, E.; Maiga, A. et al

in British Journal of Pharmacology (2010), 159

Venoms are a rich source of ligands for ion channels, but very little is known about their capacity to modulate G-protein coupled receptor (GPCR) activity. We developed a strategy to identify novel toxins ... [more ▼]

Venoms are a rich source of ligands for ion channels, but very little is known about their capacity to modulate G-protein coupled receptor (GPCR) activity. We developed a strategy to identify novel toxins targeting GPCRs. Experimental approach: We studied the interactions of mamba venom fractions with a1-adrenoceptors in binding experiments with 3H-prazosin. The active peptide (AdTx1) was sequenced by Edman degradation and mass spectrometry fragmentation. Its synthetic homologue was pharmacologically characterized by binding experiments using cloned receptors and by functional experiments on rabbit isolated prostatic smooth muscle [less ▲]

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See detailIsolation and preliminary characterisation of a filamentous terrestrial red alga.
Hoffmann, Lucien; Garbacki, Nancy ULg

Conference (1999)

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See detailIsolation and purification of bovine immunoglobins : use of Sephacryl S-300 filtration avoids protein precipitation steps.
Collard, Alfred; Pivont, P.; Portetelle, Daniel ULg et al

in Annales de Recherches Vétérinaires = Annals of Veterinary Research (1984), 15

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See detailIsolation and Purification of Panarine, a Quaternary Alkaloid from a Venezuelian Curare
Quetin-Leclercq, Joëlle; Angenot, Luc ULg; Dupont, Léon et al

in Phytochemistry (1988), 27(12), 4002-4004

High Speed Counter Current Chromatography (HSCCC) has been used to isolate and purify two quaternary alkaloids from a sample of curare prepared by the Panare Indians of Estado Bolivar, Venezuela. One of ... [more ▼]

High Speed Counter Current Chromatography (HSCCC) has been used to isolate and purify two quaternary alkaloids from a sample of curare prepared by the Panare Indians of Estado Bolivar, Venezuela. One of the compounds is the known base Macusine B. The other one is a new but related base named panarine. Its structure has been established unequivocally by spectral and X-ray cristallographic analysis. [less ▲]

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See detailIsolation and Quantification of Lactic Acid Bacteria from Traditional Fermented Products in Benin
Tchekessi, C.K.C.; Bokossa, I.Y; Azokpota, P. et al

in International Journal of Current Microbiology and Applied Sciences (2014), 3(11), 1-8

In Africa, fermented food products are particularly used as weaning foods for young children, pregnant women and the seniors. In Benin, most of these cereals-based foods are manufactured and sold around ... [more ▼]

In Africa, fermented food products are particularly used as weaning foods for young children, pregnant women and the seniors. In Benin, most of these cereals-based foods are manufactured and sold around the streets. These are ablo, dèguè, akpan, abotin, gowé etc ... This study focused on the isolation and enumeration of bacteria and yeast from twenty six (26) samples of traditional African fermented foods. Decimal dilution method allowed us to isolate a total of 42 different strains of microorganisms among which we counted 30 lactic acid bacteria. They confirmed their biological potential by expanding in selective medium MRS. When they were then subjected to screening, the medium of MRS-broth-starch agar, and eight (08) of the isolated strains tested showed their characters of amylolytic strains. The eight strains isolated were tested and finally cultivated at temperatures and pH ranging respectively from 30°C to 60°C and 3.0 to 6.5. These eight (08) amylolytic lactic acid bacteria (ALAB) synthesized enzyme amylase both inside and outside the bacterial cell with a very interesting activity value. The temperature limit between 40 and 45°C is best for the bacteria 26.2, B16 and B18 while the optimum pH for amylase synthesis is between 4.0 and 4.5 for the same microorganisms that have given the largest values of enzyme activity. The best strains 26.2, B16 and B18 secrete more enzymes in extracellular medium than intracellular. [less ▲]

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See detailIsolation and Radioimmunoassay Formation of Equine Osteocalcin- Preliminary Results
Carstanjen, B; Sulon, J; Banga-Mboko, H et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 8-9

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See detailIsolation and radioimmunoassay of a bovine pregnancy specific protein.
Beckers, Jean-François ULg; Wouters-Ballman, P.; Ectors, F.

in Theriogenology (1988, January), 29(1), 219

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See detailIsolation and sequence analysis of the rat prolactin structural gene
Martial, Joseph ULg; Cooke, N. E.

in MacLeod, Robert; Scapagnini, Umberto (Eds.) Central and peripheral regulation of prolactin function (1980)

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See detailIsolation and sequencing of Myo-Inositol Phosphate Synthase cDNA from common bean (Phaseolus vulgaris L.)
Abid, Ghassen ULg; Baudoin, Jean-Pierre ULg; Jacquemin, Jean-Marie et al

Conference given outside the academic context (2009)

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See detailIsolation and structure of akagerine: a new type of indole alkaloid
Angenot, Luc ULg; Dideberg, Otto; Dupont, Léon

in Tetrahedron Letters (1975), (16), 1357-1358

Detailed reference viewed: 46 (8 ULg)