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Peer Reviewed
See detailInvestigation of phonological abilities and phonological STM in Williams syndrome.
Majerus, Steve ULg; Palmisano, I.; Barisinikov, K. et al

Conference (2001, February)

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See detailInvestigation of potential new targets for the diagnosis and/or the treatment of osteoarthritis
Lambert, Cécile ULg; Dubuc, J.-E.; Montell, E. et al

in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013),

Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory ... [more ▼]

Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same OA patient. We identified a large number of mediators belonging to key pathways involved in OA pathogenesis. The aim of this study was to validate different potential new targets for the diagnosis and/or the treatment OA. Methods: Synovial cells (SC) were isolated from synovial specimens obtained from OA patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. The biopsies from N/R and I areas were cultured separately for a period of 7 days. Microarray gene expression profiling between N/R and I areas was performed. The biological relevance of up- and down-regulated genes was analyzed with Ingenuity Pathways Analysis. Western blot and immunohistochemistry confirmed the identified genes most differentially expressed in the key pathways. The production of the triggering receptor expressed on myeloid cells-1 (TREM1), the alarmin S100 calcium binding protein A9 (S100A9), the wingless-type MMTV integration site family, member 5A (Wnt-5A) and the stanniocalcin 1 (STC1) were evaluated by Western blot. S100A9, hyaluronan synthase-1 (HAS1) and STC1 expression and localization were investigated by immunohistochemistry. Results: 896 genes differentially expressed in N/R and I areas were identified. The key pathways were related to inflammation, cartilage metabolism, Wnt signaling and angiogenesis. In the inflammatory gene pattern, TREM1 and S100A9 were strongly upregulated. We validated the production of these proteins in OA synovial biopsies by Western blot. TREM1 and S100A9 were increased in I compared to N/R synovial cells culture. S100A9 was observed in the perivascular area and in sublining cells in I synovial biopsies, but not in N/R biopsies. An increased staining was also observed in the intima lining layer of I when compared to N/R biopsies. The most upregulated anabolism enzyme in I synovial biopsies was HAS1. Using immunohistochemistry, we observed in I areas an increase of the HAS1-positive cells mainly in the intima lining. We also studied the protein production of Wnt-5A, the most upregulated intermediate of Wnt signaling pathway. The protein level was increased in I compared to N/R areas. Finally, in the angiogenesis pathway, one the most u-regulated gene was STC1. A significant increase of STC1 production was observed in I areas compared to N/R areas by Western blot. This result was also supported by the immunohistochemical analysis. In I area, the staining for STC1 was more intense in perivascular and sublining cells. Conclusions: Synovial membrane inflammation is a key target for OA treatments. In this work, we have identified proteins involved in the synovitis pathways like angiogenesis, cells infiltration and matrix remodeling. These proteins could be targeted by drugs and used as companion biomarkers for evaluating their efficacy. Although qualitative, our results could also yield to the identification of markers of the disease. This investigation has to be further pursued. [less ▲]

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See detailInvestigation of pregnancy-associated glycoproteins (PAGs) by means of an enzymoimunoassay (ELISA) sandwich kit for pregnancy monitoring in sheep
El Amiri, Bouchra; Delahaut, Philippe; Colemans, Yves et al

in Chentouf, M; López-Francos, A; Bengoumi, M (Eds.) et al Options méditerranéennes : Series A (2014)

The aim of the present study was to investigate the use of a PAGs ELISA-Sandwich kit (Ret. code E.G.7. CER. Marloie, Belgium) for both early pregnancy diagnosis (in Sardi sheep) and the pregnancy follow ... [more ▼]

The aim of the present study was to investigate the use of a PAGs ELISA-Sandwich kit (Ret. code E.G.7. CER. Marloie, Belgium) for both early pregnancy diagnosis (in Sardi sheep) and the pregnancy follow up (in Boujaâd and Boujaâd x D'man sheep). ln Sardi breed, plasma samples were obtained from pregnant ewes (n = 17) from day 18 to 30 of gestation at 2 days interval. ln Boujaâd (n = 8) and Boujaâd x D'man (n = 20) the blood samples were weekly collected from the first week of gestation till the 4th week after lambing. The PAG concentrations were determined by a sandwich-ELISA based on purified bovine PAG (boPAG-67 kDa) as a standard, the antiserum raised against caprine PAG (caPAG-55+62 kDa) as a capture antibody (1/40000) and antiserum raised against purified PAG from buffalo (AS 859) as detection antibody (1/32 000). The Avidin-HRP and TMB were used to reveal reactions. Ewes were assumed to be pregnant when PAG concentrations were higher than 0.8 ng/ml. Results showed that in Sardi sheep it is possible to detect ail ewes as pregnant as early as on 24 days of gestation. ln Boujaâd and Boujaâd x D'man the PAG concentrations reached the higher values just before lambing. This value is higher in ewes carrying more than one lamb than in those carrying a single lamb. To conclude, the present study shows that the ELISA kit used for early pregnancy diagnosis in sheep as weil as for pregnancy fellow up could be a good alternative to the radioimmunoassay RIA in countries where this last technique is hard to set up. [less ▲]

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See detailInvestigation of pulmonary expression of heat shock protein HSP70 in mice chronically exposed to cigarette smoke
Cheu, Esteban ULg; Steuve, J.; Fievez, Laurence ULg et al

in Proceedings: Autumn Meeting of the Belgian Society of Fundamental and Clinical Physiology and Pharmacology (2007)

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See detailInvestigation of SDS, DTAB and CTAB micelle microviscosities by electron spin resonance
Bahri, Mohamed Ali ULg; Hoebeke, Maryse ULg; Grammenos, Angeliki ULg et al

in Colloids and Surfaces A : Physicochemical and Engineering Aspects (2006), 290(1-3), 206-212

Electron spin resonance spectroscopy (ESR) of the nitroxide labelled fatty acid probes (5-, 16-doxyl stearic acid) was used to monitor the micelle microviscosity of three surfactants at various ... [more ▼]

Electron spin resonance spectroscopy (ESR) of the nitroxide labelled fatty acid probes (5-, 16-doxyl stearic acid) was used to monitor the micelle microviscosity of three surfactants at various concentrations in aqueous solution: sodium dodecyl sulphate (SDS), dodecyltrimethylammonium bromide (DTAB) and cetyltrimethylammonium bromide (CTAB). At low surfactant concentration, there is no micelle, the ESR probe is dissolved in water/surfactant homogeneous phase and gives his microviscosity. At higher surfactant concentration, an abrupt increase in microviscosity indicates the apparition of micelles and, the solubilization of the probes in micelles. The microviscosity of the three surfactants, in a large surfactant range, was obtained as well as the critical micelle concentration (CMC). The microviscosity increased slightly with the increase in surfactant concentration. Phosphate buffer lowered the CMC value and generally increased the microviscosity. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailInvestigation of shear stress evolution during neotissue growth in a perfusion bioreactor using 3d multiphysics modeling
Guyot, Yann ULg; Papantoniou, Ioannis; Schrooten, Jan et al

Conference (2014, October)

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See detailInvestigation of singlet oxygen reactivity towards propofol
Heyne, Belinda; Kohnen, Stephan ULg; Brault, Daniel et al

in Photochemical & Photobiological Sciences (2003), 2(9), 939-945

The reaction between the anaesthetic agent 2,6-diisopropylphenol (propofol, PPF) and singlet oxygen (1O2) has been investigated in aqueous solution by means of HPLC, GC, absorption spectroscopy and laser ... [more ▼]

The reaction between the anaesthetic agent 2,6-diisopropylphenol (propofol, PPF) and singlet oxygen (1O2) has been investigated in aqueous solution by means of HPLC, GC, absorption spectroscopy and laser flash photolysis with infrared luminescence detection. The rate constants for the physical and chemical quenching of 1O2 by PPF (kPPF) are found to be 2.66 x 10(5) M(-1) s(-1) and approximately 3.2 x 10(6) M(-1) s(-1) in CD3OD and D2O-CD3OD (75:25 v/v), respectively. The reaction of propofol with singlet oxygen produced by light irradiation of Rose Bengal leads essentially to two reaction products, 2,6-diisopropyl-p-benzoquinone and 3,5,3',5'-tetraisopropyl-(4,4')-diphenoquinone that are unambiguously identified from comparison with authentic samples. [less ▲]

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See detailInvestigation of soil degradation by means of an opto-electronic microreliefmeter
Destain, Marie-France ULg; Descornet, G.; Roisin, C. et al

in Soil & Tillage Research (1989), 13(3), 299-315

To investigate the behaviour of seedbeds as affected by climatic stresses, under the dependance of different systems of organic improvements, an opto-elctronic device was used, presenting a high ... [more ▼]

To investigate the behaviour of seedbeds as affected by climatic stresses, under the dependance of different systems of organic improvements, an opto-elctronic device was used, presenting a high resolution capacity and good rapidity. The contactless measurements ditrectly recorded in a computer have been repeated at different times exactly at the same place in the field. A complete statistical analysis by means of spectrum analysis gave accurate information about the behaviour of clods of different size. The differentiated evolution of the soil surface according to climatic stresses (frost and rainfall) was noted. The influence of organic and/or mineral improveemnts appeared clearly too. [less ▲]

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See detailInvestigation of sol-gel yttrium doped ZnO thin films: structural and optical properties
Ivanova, T; Harizanova, A; Koutzarova, T et al

in Journal of Physics: Conference Series (2016)

Nanostructured metal oxide films are extensively studied due to their numerous applications such as optoelectronic devices, sensors. In this work, we report the Y–Zn–O nanostructured films prepared by sol ... [more ▼]

Nanostructured metal oxide films are extensively studied due to their numerous applications such as optoelectronic devices, sensors. In this work, we report the Y–Zn–O nanostructured films prepared by sol-gel technology from sols with different concentration of yttrium precursor, followed by post-annealing treatment. The Y doped ZnO thin films have been deposited on Si and quartz substrates by spin coating method, then treated at températures ranging from 300-800°C. XRD analysis reveals modification of the film structure and phases in the doped ZnO films. [less ▲]

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See detailInvestigation of spectral impacts on the performance of a concentration device using a Fresnel lens combined with a double junction cell
Loicq, Jerôme ULg; Galante, Nicolas ULg; Thibert, Tanguy ULg et al

in Proceedings of SPIE (2014, May 15)

This experimental study was carried out within the context of high concentration photovoltaics. The paper presents the results of an experimental investigation relating to the quantification of the ... [more ▼]

This experimental study was carried out within the context of high concentration photovoltaics. The paper presents the results of an experimental investigation relating to the quantification of the impacts of the chromatic effect on the performance of a double junction GaInP/GaAs solar cell. Chromatic effects are the result of material dispersion caused by the refractive optics component. This study aims to evaluate the effect of the spectral modification of the incident beam on the whole solar concentrator system performance. Such considerations are fundamental in producing a highly accurate design, with which to achieve the best possible system performance. Efficiency is evaluated within the vicinity of the focus of a Fresnel lens designed for concentration. On the optical axis, rays with different wavelengths are not focalized at the same points. The spectral content of the beam depends, therefore, upon the position of the cell along the optical axis. It is assumed that spectral content modification may have an impact on cell performance and, as a consequence, on system efficiency as a whole. Efficiency of the optical Fresnel lens and of the cell were evaluated in relation to spectral content modification. [less ▲]

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See detailAn investigation of structural dimension variation in electrostatically-coupled MEMS resonator pairs using mode-localization
Wood, Graham; Chun, Zhao; Pu, Suan et al

in IEEE Sensors Journal (2016)

If a pair of MEMS resonators are electrostatically coupled together, the vibration amplitude ratios at the resonant frequencies of the resulting coupled system are sensitive to stiffness perturbation. An ... [more ▼]

If a pair of MEMS resonators are electrostatically coupled together, the vibration amplitude ratios at the resonant frequencies of the resulting coupled system are sensitive to stiffness perturbation. An imbalance between the two resonators causes the confinement of vibration energy when the system is resonating, an effect known as mode-localization. The degree of localization can be determined by extracting the amplitude ratio of the resonators through capacitive transduction. In this paper, we have fabricated MEMS devices, using a dicing-free silicon-on-insulator process, consisting of pairs of closely spaced microresonators. Each resonator consists of a clamped-clamped beam with a wider section in the middle, which is the location of the electrostatic coupling, instituted through the DC biasing of the resonators. Several devices have been fabricated, with the length of the anchor beams being varied, which influences the frequency of resonance. Stiffness imbalance between the resonators has been introduced through electrostatic spring softening, with the sensitivity of the amplitude ratio of the resonant mode shape being greater for the higher frequency, shorter anchor devices. The sensitivities of the devices in this study have been found to be 9 times greater than state-of-the-art two-degree-of-freedom mode-localized sensors. [less ▲]

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See detailInvestigation of Structure-Fundamental/Functional Property Relationships of (Bio)-surfactants for a Rational Design of their Preparation and Use in Food and Non-food applications
Razafindralambo, Hary ULg

Conference (2012, May 01)

(Bio)-surfactants are among the most versatile compounds in our planet, either in nature or in biological systems, as well as in manufactured products. Increasing knowledge on the relationships between ... [more ▼]

(Bio)-surfactants are among the most versatile compounds in our planet, either in nature or in biological systems, as well as in manufactured products. Increasing knowledge on the relationships between their structure, fundamental properties, and functionalities in simple, and thereafter in more complicated models, are therefore the key factor on their rational preparation and use for any applications. In the present communication, the effect of different structural entities of (bio)surfactants, especially Carbohydrate-based amphiphilic compounds, including monomeric, oligomeric, and polymeric surfactants, on their interfacial properties at short time (under dynamic conditions) and long time (at the equibrium), and the impact of such fundamental properties through physico-chemical parameters on the formation and stability of colloidal systems (micelles, foams, emulsions) will be presented. Some perspectives in their applications will be discussed. [less ▲]

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See detailInvestigation of sudden death of calves after epileptic seizures due to hypomagnesaemia and hyperparathyroidism
Chevalier, Marine ULg; Lebreton, Pascal; Garnier, Catherine et al

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

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See detailInvestigation of the Adaptability of Transient Stability Assessment Methods to Real-Time Operation
Weckesser, Johannes ULg; Jóhannsson, Hjörtur; Sommer, Stefan et al

in IEEE PES Innovative Smart Grid Technologies Conference Europe (2012)

In this paper, an investigation of the adaptability of available transient stability assessment methods to real-time operation and their real-time performance is carried out. Two approaches based on ... [more ▼]

In this paper, an investigation of the adaptability of available transient stability assessment methods to real-time operation and their real-time performance is carried out. Two approaches based on Lyapunov’s method and the equal area criterion are analyzed. The results allow to determine the runtime of each method with respect to the number of inputs. Furthermore, it allows to identify, which method is preferable in case of changes in the power system such as the integration of distributed power resources (DER). A comparison of the performance of the analyzed methods leads to the suggestion that matrix reduction and time domain simulation are the most critical operations. [less ▲]

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See detailInvestigation of the added mass method for seismic design of lock gates
Buldgen, Loïc ULg; Le Sourne, Hervé; Caprace, Jean-David ULg et al

in Engineering Structures (2017)

Detailed reference viewed: 13 (4 ULg)