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See detailAssessment of reactive oxygen species production in cultured equine skeletal myoblasts in response to conditions of anoxia followed by reoxygenation with or without exposure to peroxidases.
Ceusters, Justine ULg; Mouithys-Mickalad, Ange ULg; de la Rebière de Pouyade, Geoffroy ULg et al

in American Journal of Veterinary Research (2012), 73(3), 426-434

Objective—To culture equine myoblasts from muscle microbiopsy specimens, examine myoblast production of reactive oxygen species (ROS) in conditions of anoxia followed by reoxygenation, and assess the ... [more ▼]

Objective—To culture equine myoblasts from muscle microbiopsy specimens, examine myoblast production of reactive oxygen species (ROS) in conditions of anoxia followed by reoxygenation, and assess the effects of horseradish peroxidase (HRP) and myeloperoxidase (MPO) on ROS production. Animals—5 healthy horses (5 to 15 years old). Procedures—Equine skeletal myoblast cultures were derived from 1 or 2 microbiopsy specimens obtained from a triceps brachii muscle of each horse. Cultured myoblasts were exposed to conditions of anoxia followed by reoxygenation or to conditions of normoxia (control cells). Cell production of ROS in the presence or absence of HRP or MPO was assessed by use of a gas chromatography method, after which cells were treated with a 3,3′-diaminobenzidine chromogen solution to detect peroxidase binding. Results—Equine skeletal myoblasts were successfully cultured from microbiopsy specimens. In response to anoxia and reoxygenation, ROS production of myoblasts increased by 71%, compared with that of control cells. When experiments were performed in the presence of HRP or MPO, ROS production in myoblasts exposed to anoxia and reoxygenation was increased by 228% and 183%, respectively, compared with findings for control cells. Chromogen reaction revealed a close adherence of peroxidases to cells, even after several washes. Conclusions and Clinical Relevance—Results indicated that equine skeletal myoblast cultures can be generated from muscle microbiopsy specimens. Anoxia-reoxygenation– treated myoblasts produced ROS, and production was enhanced in the presence of peroxidases. This experimental model could be used to study the damaging effect of exercise on muscles in athletic horses. [less ▲]

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See detailAssessment of residual insulin secretion in diabetic patients using the intravenous glucagon stimulatory test: methodological aspects and clinical applications.
Scheen, André ULg; Castillo, M. J.; Lefebvre, Pierre ULg

in Diabètes & Métabolism (1996), 22(6), 397-406

Defective insulin secretion plays a crucial role in insulin-dependent (Type 1) and non-insulin-dependent (Type 2) diabetes mellitus as well as in many secondary forms of the disease. Glucagon is a potent ... [more ▼]

Defective insulin secretion plays a crucial role in insulin-dependent (Type 1) and non-insulin-dependent (Type 2) diabetes mellitus as well as in many secondary forms of the disease. Glucagon is a potent stimulus for the islet beta-cell, and intravenous bolus injection of 1 mg glucagon has been widely used to assess endogenous insulin secretion for clinical or research purposes. Plasma C-peptide levels (less commonly insulin) are usually measured immediately before and 6 min after glucagon injection. The C-peptide response to glucagon is well-correlated with the beta-cell response to mixed meals or other stimuli commonly used to characterize endogenous insulin secretion (oral or intravenous glucose, standard meals, arginine, etc.) and has the advantage of shorter duration and simple standardization. The glucagon test shows good intra-subject reproducibility, although in diabetic patients it may be influenced by variable prevailing blood glucose levels. Several applications of the glucagon test have been developed. In Type 1 diabetes, the glucagon test has been used to discriminate between patients with and without residual insulin secretion. This can be especially important during the first few months, or even years, following initiation of insulin therapy when attempts to stop the immunological destruction of the beta-cell are made. Assessment of endogenous insulin secretion is also important after pancreas or islet transplantation. In patients with Type 2 diabetes mellitus, in which residual endogenous insulin secretion is common, characterization of the disease may help in the choice of therapy for the individual patient (insulin, sulphonylureas or combined therapy). Thus, the glucagon test is a simple, reliable and useful tool for clinical evaluation of diabetes mellitus. [less ▲]

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See detailAssessment of respiratory diseases and therapeutic intervention by the forced oscillation technique in feedlot cattle
Gustin, Pascal ULg; Landser, F. J.; Lomba, F. et al

in Research in Veterinary Science (1990), 49(3), 319-322

The forced oscillation technique was used to study pulmonary function in bulls during naturally acquired pulmonary-gastrointestinal disease complex known as shipping fever and as it was treated with two ... [more ▼]

The forced oscillation technique was used to study pulmonary function in bulls during naturally acquired pulmonary-gastrointestinal disease complex known as shipping fever and as it was treated with two different antibiotics. Fifteen double-muscled bulls of the Belgian White and Blue breed were investigated. Clinical, serological and pulmonary function values were recorded during and after naturally occurring respiratory disease. Total respiratory resistance (Rrs) and reactance (Xrs) were measured by the forced oscillation technique. The antibiotic ceftiofur (group A, n = 10) and oxytetracycline (group B, n = 5) were given over three days. A large increase of Rrs at low frequencies with a marked negative frequency dependence, an increase of the resonant frequency, and a decrease of Xrs were recorded in the initial stage of the disease. The parameters returned to normal values seven days later. Clinical evidence of recovery was more marked in group A than in group B. These results show that moderate shipping fever induces acute small and large airway obstruction which responds to appropriate antibiotic therapy. [less ▲]

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See detailAssessment of respiratory mechanics with impulse oscillometry in horses with upper and lower airway obstruction
Van Erck, Emmanuelle; Duvivier, D. H.; Art, Tatiana ULg et al

in Plügers Archives European Journal of Physiology (1998)

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See detailAssessment of respiratory muscle performance in non cooperative subjects : a preliminary study
Desmecht, Daniel ULg; Art, Tatiana ULg; Amory, Hélène ULg et al

in Proceedings of the 7th Comparative Respiratory Society Meeting (1988)

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See detailASSESSMENT OF RETAINED AUSTENITIC PHASE IN INDEFINITE CHILL CAST IRON MICROSTRUCTURE TOWARDS VARIOUS TECHNIQUES
Tchuindjang, Jérôme Tchoufack ULg; Terziev, Liuben; Sinnaeve, Mario et al

Poster (2007, November 16)

Indefinite Chill Cast Irons also called ICDP when used as rolling mill rolls are high alloyed cast iron alloys belonging to the Fe-Cr-C system. ICDP exhibit improved properties regarding hot oxidation and ... [more ▼]

Indefinite Chill Cast Irons also called ICDP when used as rolling mill rolls are high alloyed cast iron alloys belonging to the Fe-Cr-C system. ICDP exhibit improved properties regarding hot oxidation and wear resistances as their microstructure is a mixture of a martensitic with bulky cementite (M3C) and lamellar graphite fully dispersed into the matrix. Mechanical properties involved depend either on the chemical composition of the alloy or the crystallisation behaviour and the heat treatments performed subsequently in order to lead to the desired microstructure. Thus hardenability of the matrix is of great concern as heat treatments sequence is intended to transform austenite in a more stable structure like martensite or even bainite, depending on Cr content. In fact austenite is known to be unstable during hot working conditions as hot temperature and external stresses could yield transformation of this phase into martensite with major volume distortions and even multiple cracks network on the component involved. To avoid such a hitch in service it is quite necessary to make sure all the austenite is completely transformed into martensite while performing subsequent heat treatments like quenching or tempering on the material obtained after casting process. Various techniques are allowable starting from classical ones (X-Ray Diffraction (XRD), Image Analysis, Vibrating Sample Magnetometer (VSM), Dilatometry) to more exotic one like Conversion Electron Mössbauer Spectroscopy (CEMS). From an industrial point of view, it is important to set a fast and reliable method to assess retained austenite presence into ICDP microstructure at the end of any step of the thermomechanical process. The present work is intended to compare different techniques used for retained austenite assessment in ICDP alloys as a first step prior to the calibration of a classical method like dilatometry. This calibration is to be set starting from complex XRD and CEMS spectrometers analyses. Dilatometry seems to overrate retained austenite volume fraction in the as-cast conditions while good agreements are made on the overall batch of techniques when considering heat treated material where retained austenite transformation occurred [less ▲]

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See detailAssessment of sea bass swimming activity and preferential space use in sea cages using acoustic telemetry and archival tags
Faucher, Karine ULg; Millot, Sandie ULg; Struski, Caroline et al

Poster (2007, June)

Swimming activity rhythms and levels as well as space occupation by sea bass, Dicentrarchus labrax, in a sea cage (6.5 x 6.5 x 8 m, ca. 25000 fish of 400 g stocked at 30 kg m-3) were recorded using ... [more ▼]

Swimming activity rhythms and levels as well as space occupation by sea bass, Dicentrarchus labrax, in a sea cage (6.5 x 6.5 x 8 m, ca. 25000 fish of 400 g stocked at 30 kg m-3) were recorded using acoustic telemetry (9 fish, IBDT Sonotronics) and archival tags (11, DSTs STAR ODDI). Acoustic tracking was realized over a 13 days period and successful for 3 individuals (one fix every 2 min on average). For archival tags, only 4 tags were retrieved and 3 had been recording swimming depth and temperature for 9 mo (Nov 05-June 06). Telemetry results showed an even day/night swimming activity levels in November with fish staying in close association with the bottom. Archival tags recordings revealed a preferential distribution in the water column between mid-water and bottom of the net cage in winter, over the whole water column in spring and near the surface zone in early-summer. In addition, on a 24 hrs period basis, sea bass presented a vertical movement rhythm: they swam closer to the surface during the day, specially around feeding events, and near the bottom at night. That vertical movement was pronounced during winter, faded during spring and almost disappeared in summer. [less ▲]

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See detailAssessment of Solvent Degradation within a Global Process Model of Post-Combustion CO2 Capture
Léonard, Grégoire ULg; Toye, Dominique ULg; Heyen, Georges ULg

in Computer Aided Chemical Engineering (in press), 33

Solvent degradation may be a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. However, its influence on the ... [more ▼]

Solvent degradation may be a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. However, its influence on the process operations has rarely been studied. In the present work, a kinetics model describing solvent oxidative and thermal degradation has been developed based on own experimental results for the benchmark solvent, i.e. 30 wt% monoethanolamine (MEA) in water. This model has been included into a global Aspen Plus model of the CO2 capture process. The selected process modelling approaches are described in the present work. Using the resulting simulation model, optimal operating conditions can be identified to minimize both the energy requirement and the solvent degradation in the process. This kind of process model assessing solvent degradation may contribute to the design of large-scale CO2 capture plants to consider not only the process energy penalty, but also its environmental penalty. Indeed, both aspects are relevant for the large-scale deployment of the CO2 capture technology. [less ▲]

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See detailAssessment of space radiation effects on solid-state Brillouin phase conjugate mirrors
Brignon, Arnaud; Richard, Sébastien; Gusarov, Andreï et al

in Applied Optics (2007), 46(22), 5329-5335

Optical phase conjugation (OPC) provides a means of dynamical correction of thermally induced aberrations in high-power laser systems. This method is particularly interesting for space applications ... [more ▼]

Optical phase conjugation (OPC) provides a means of dynamical correction of thermally induced aberrations in high-power laser systems. This method is particularly interesting for space applications because it is passive, mechanically simple, and allows for improved beam quality with only a small loss in power. To exploit it one has to assess the effect of the space environment, and in particular of space radiation, on the properties of the materials that are suitable for phase-conjugating mirrors (PCMs). We have investigated both materials providing OPC via stimulated Brillouin scattering and actual PCMs with regard to their radiation hardness. Proton- and gamma-irradiated PCMs in the form of 30-cm long silica rods and 5-cm long TeO2 crystals were tested in a single frequency flash-lamp pumped Nd:YAG system delivering up to 220 mJ pulses with a 20 ns duration at a 50 Hz repetition rate. The difference in the reflectivity between irradiated and nonirradiated components was found to be within the measurement errors. Gamma irradiation of TeO2 resulted in a decrease of the laser-induced damage threshold, while for silica possible changes were below the detection limit. Our results show that synthetic fused silica, and Lithosil in particular, can be used for generating the OPC in laser systems operating in a space radiation environment. [less ▲]

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See detailAssessment of stress level in horses (Equus caballus): behavioural and physiological measurements in hospital.
Peeters, Marie ULg; Péters, F.; Sulon, J. et al

Conference (2008)

Detailed reference viewed: 38 (7 ULg)
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See detailAssessment of stress level in horses during competition using salivary cortisol: preliminary studies
Peeters, Marie ULg; Sulon, Joseph; Serteyn, Didier ULg et al

in Journal of Veterinary Behavior : Clinical Applications and Research (2010, July), 5(4), 216

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See detailAssessment of structure-modifying drugs in osteoarthritis: surrogate or hard clinical end points?
Bruyère, Olivier ULg; Reginster, Jean-Yves ULg

in Future Rheumatology (2006), 1(2), 199-206

Current regulatory requirements for the development of disease-modifying drugs in osteoarthritis (OA) still consider the measurement of joint space narrowing (a change in joint space width over many years ... [more ▼]

Current regulatory requirements for the development of disease-modifying drugs in osteoarthritis (OA) still consider the measurement of joint space narrowing (a change in joint space width over many years) on plain x-rays as the appropriate primary end point for the demonstration of efficacy. However, other outcomes have recently been proposed. Among these, time to joint replacement appears to be an attractive option. Preliminary results are encouraging; however, more data are needed before recommending joint replacement as a primary outcome in disease-modifying OA drugs. It should be acknowledged that, owing to potential biases (e.g., absence of recommended guidelines, socio–economic and gender disparities in the rates of knee surgery, variability from country to country and willingness of patients to undergo the surgery), a recent expert consensus panel did not recommend this particular outcome as the primary end point for assessing the success or failure of a disease-modifying drug. Other tools, such as magnetic resonance imaging or biochemical markers, have potential as outcome measures but more studies are needed. [less ▲]

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See detailAssessment of systolic myocardial function in conscious healthy dogs during dobutamine infusion
Mc Entee, K; Amory, Hélène ULg; Pypendop, B et al

in In Proceedings of the 6th ESVIM Congress (1996)

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See detailAssessment of the 3D Localization of Metallic Nanoparticles in Pd/SiO2 Cogelled Catalysts by Electron Tomography
Gommes, Cédric ULg; de Jong, Krijn; Pirard, Jean-Paul ULg et al

in Langmuir (2005), 21

The purpose of this study is to analyze the localization of palladium nanoparticles within their silica support, in two heterogeneous catalysts synthesized by the sol-gel process, with different metal ... [more ▼]

The purpose of this study is to analyze the localization of palladium nanoparticles within their silica support, in two heterogeneous catalysts synthesized by the sol-gel process, with different metal loadings. Electron tomography demonstrates that the palladium particles are localized deep inside the silica skeleton. The use of digital image analysis further shows that the dispersion of palladium is optimal in the sample with the lowest loading. The particles are regularly spaced in the middle of the silica skeleton, with a distance between them comparable to the diameter of the struts of silica. [less ▲]

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See detailAssessment of the acaricidal activity of several plant extracts on the phytophagous mite Tetranychus urticae (Tetranychidae) in Tunisian citrus orchards
Attia, Sabrine; Grissa, Kaouthar Lebdi; Zeineb, Ghrabi-Gammar et al

in Bulletin de la Société Royale Belge d'Entomologie = Bulletin van de Koninklijke Belgische Vereniging voor Entomologie (2011), 147(I-IV), 71-79

To develop sustainable pest control in Tunisian citrus orchards, the present work aimed to evalute the toxicity of 31 plant extracts obtained from Tunisia and two synthetic acaricides (spirodiclofen and ... [more ▼]

To develop sustainable pest control in Tunisian citrus orchards, the present work aimed to evalute the toxicity of 31 plant extracts obtained from Tunisia and two synthetic acaricides (spirodiclofen and fenbutatin oxide) on the phytophagous mite species Tetranychus urticae (Koch). [less ▲]

Detailed reference viewed: 84 (3 ULg)