Proteomic investigation of aphid honeydew reveals an unexpected diversity of proteins
Vandermoten, Sophie ; ; et al
in PLoS ONE (2013), Accepted pending revisionDetailed reference viewed: 11 (0 ULg)
Proteomic kinetic analysis of blister fluid and serum in a patient with drug-induced toxic epidermal necrolysis. A comparison with skin immunohistochemistry.
; Meuwis, Marie-Alice ; Mazzucchelli, Gabriel et al
in Current Drug Safety (2012), 7(5), 339-51
Drug-induced toxic epidermal necrolysis (TEN) is a rare but potentially lethal bullous disease whose complex pathomechanisms remain uncertain. The aim of the study was an exploratory attempt to assess TEN ... [more ▼]
Drug-induced toxic epidermal necrolysis (TEN) is a rare but potentially lethal bullous disease whose complex pathomechanisms remain uncertain. The aim of the study was an exploratory attempt to assess TEN pathobiology using a combination of immunohistochemistry and proteomics. Skin blister fluid (BF) and serum were collected in a patient in the early TEN stage at day (D) +4 of evolution and three days later (D +7). Intravenous cyclosporine A (CsA) treatment was initiated since D +4. Immunohistochemistry was performed on skin blister biopsies. In addition, proteomic analyses compared the BF protein content before and at the issue of the 3-day CsA treatment. Proteins were selected according to their prominent differential abundance in BF between D+4 and D+7, when influenced by lesional skin cells, but not in serum. Among 300 proteins, four were considered. Glutathione transferase pi was related to oxidative stress in TEN epidermis. The monocyte differentiation antigen CD14 and myeloperoxidase indicated macrophage activation. The proinflammatory S100-A8 protein probably originated from activated keratinocytes and/or macrophages. These proteomic findings were in line with immunohistochemistry and supported the prominent involvement of keratinocytes and macrophages in TEN pathomechanism. As opposed to CD14, other proteins were mainly present in BF at D+7, confirming that CsA expressed little effect, if any, on the activity of keratinocytes and macrophages in the present TEN patient. Of note, the present exploratory study using proteomic analyses in a single TEN case supports a pathogenic hypothesis without establishing any firm conclusion. [less ▲]Detailed reference viewed: 15 (5 ULg)
Proteomic mass spectra classification using decision tree based ensemble methods.
Geurts, Pierre ; Fillet, Marianne ; De Seny, Dominique et al
in Bioinformatics (2005), 21(14), 3138-45
MOTIVATION: Modern mass spectrometry allows the determination of proteomic fingerprints of body fluids like serum, saliva or urine. These measurements can be used in many medical applications in order to ... [more ▼]
MOTIVATION: Modern mass spectrometry allows the determination of proteomic fingerprints of body fluids like serum, saliva or urine. These measurements can be used in many medical applications in order to diagnose the current state or predict the evolution of a disease. Recent developments in machine learning allow one to exploit such datasets, characterized by small numbers of very high-dimensional samples. RESULTS: We propose a systematic approach based on decision tree ensemble methods, which is used to automatically determine proteomic biomarkers and predictive models. The approach is validated on two datasets of surface-enhanced laser desorption/ionization time of flight measurements, for the diagnosis of rheumatoid arthritis and inflammatory bowel diseases. The results suggest that the methodology can handle a broad class of similar problems. [less ▲]Detailed reference viewed: 55 (14 ULg)
Proteomic study of plant-aphid interactions: between defense and adaptations.
Francis, Frédéric ; ; De Pauw, Edwin et al
in Comparative Biochemistry and Physiology. Part A, Physiology (2005), 141Detailed reference viewed: 19 (5 ULg)
Proteomics for prediction and characterization of response to infliximab in Crohn's disease: a pilot study.
Meuwis, Marie-Alice ; Fillet, Marianne ; Lutteri, Laurence et al
in Clinical Biochemistry (2008), 41(12), 960-7
OBJECTIVES: Infliximab is the first anti-TNFalpha accepted by the Food and Drug Administration for use in inflammatory bowel disease treatment. Few clinical, biological and genetic factors tend to predict ... [more ▼]
OBJECTIVES: Infliximab is the first anti-TNFalpha accepted by the Food and Drug Administration for use in inflammatory bowel disease treatment. Few clinical, biological and genetic factors tend to predict response in Crohn's disease (CD) patient subcategories, none widely predicting response to infliximab. DESIGN AND METHODS: Twenty CD patients showing clinical response or non response to infliximab were used for serum proteomic profiling on Surface Enhanced Lazer Desorption Ionisation-Time of Flight-Mass Spectrometry (SELDI-TOF-MS), each before and after treatment. Univariate and multivariate data analysis were performed for prediction and characterization of response to infliximab. RESULTS: We obtained a model of classification predicting response to treatment and selected relevant potential biomarkers, among which platelet aggregation factor 4 (PF4). We quantified PF4, sCD40L and IL-6 by ELISA for correlation studies. CONCLUSIONS: This first proteomic pilot study on response to infliximab in CD suggests association between platelet metabolism and response to infliximab and requires validation studies on a larger cohort of patients. [less ▲]Detailed reference viewed: 94 (25 ULg)
Proteomics in Myzus persicae: effect of aphid host plant switch.
Francis, Frédéric ; ; Harmel, Nicolas et al
in Insect Biochemistry and Molecular Biology (2006), 36(3), 219-27
Chemical ecology is the study of how particular chemicals are involved in interactions of organisms with each other and with their surroundings. In order to reduce insect attack, plants have evolved a ... [more ▼]
Chemical ecology is the study of how particular chemicals are involved in interactions of organisms with each other and with their surroundings. In order to reduce insect attack, plants have evolved a variety of defence mechanisms, both constitutive and inducible, while insects have evolved strategies to overcome these plant defences (such as detoxification enzymes). A major determinant of the influence of evolutionary arms races is the strategy of the insect: generalist insect herbivores, such as Myzus persicae aphid, need more complex adaptive mechanisms since they need to respond to a large array of different plant defensive chemicals. Here we studied the chemical ecology of M. persicae associated with different plant species, from Brassicaceae and Solanaceae families. To identify the involved adaptation systems to cope with the plant secondary substances and to assess the differential expression of these systems, a proteomic approach was developed. A non-restrictive approach was developed to identify all the potential adaptation systems toward the secondary metabolites from host plants. The complex protein mixtures were separated by two-dimensional electrophoresis methods and the related spots of proteins significantly varying were selected and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Fourteen aphid proteins were found to vary according to host plant switch; ten of them were down regulated (proteins involved in glycolysis, TCA cycle, protein and lipid synthesis) while four others were overexpressed (mainly related to the cytoskeleton). These techniques are very reliable to describe the proteome from organisms such as insects in response to particular environmental change such as host plant species of herbivores. [less ▲]Detailed reference viewed: 51 (7 ULg)
Proteomics of life at low temperatures: trigger factor is the primary chaperone in the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125
Piette, Florence ; D'Amico, Salvino ; Struvay, Caroline et al
in Molecular Microbiology (2010), 76(1), 120-132Detailed reference viewed: 97 (53 ULg)
Proteomics to identify odorant binding proteins (OBPs) and Chemosensory Proteins (CSPs) from the Antennae and Tarsi of Tribolium brevicornis
; Francis, Frédéric ; De Pauw, Edwin et al
in Abstract book (2006)Detailed reference viewed: 20 (5 ULg)
Protéomique et entomologie moléculaire : étude des relations plantes – pucerons
Francis, Frédéric ; Mazzucchelli, Gabriel ; De Pauw, Edwin et al
in Proceedings Congrès SFEAP (2007)Detailed reference viewed: 19 (6 ULg)
Protéomique par SELDI-TOF-MS des maladies inflammatoires articulaires: identification des protéines S100 comme protéines d'intérêt
De Seny, Dominique ; Ribbens, Clio ; Cobraiville, Gaël et al
in Revue Médicale de Liège (2009), 64(Spec No), 29-35
Clinical proteomics is a technical approach studying the entire proteome expressed by cells, tissues or organs. It describes the dynamics of cell regulation by detecting molecular events related to ... [more ▼]
Clinical proteomics is a technical approach studying the entire proteome expressed by cells, tissues or organs. It describes the dynamics of cell regulation by detecting molecular events related to diseases development. Proteomic techniques focus mainly on identification of new biomarkers or new therapeutic targets. It is a multidisciplinary approach using medical, biological, bioanalytical and bioinformatics knowledges. A strong collaboration between these fields allowed SELDI-TOF-MS proteomics studies to be performed at the CHU and the University of Liege, in GIGA-Research facilities. The aim of these studies was driven along three main axes of research related to the identification of biomarkers specific to a studied pathology, to a common biological pathway and, finally, to a treatment response. [less ▲]Detailed reference viewed: 57 (5 ULg)
Proteone analysis of the bovine milk fat globule : enhancement in the membrane purification.
Vanderghem, Caroline ; Blecker, Christophe ; Danthine, Sabine et al
Poster (2007, May 15)Detailed reference viewed: 9 (1 ULg)
Proteus syndrome of the hand.
; ; Pierard, Gérald
in Dermatology : International Journal for Clinical & Investigative Dermatology (2002), 204(4), 318-20
Proteus syndrome exhibits an expanded phenotype. We report a case with restricted macrodactyly and cerebriform hypertrophy of one palm. The differential diagnosis with macrodactyly and other hamartomatous ... [more ▼]
Proteus syndrome exhibits an expanded phenotype. We report a case with restricted macrodactyly and cerebriform hypertrophy of one palm. The differential diagnosis with macrodactyly and other hamartomatous overgrowth syndromes is discussed. [less ▲]Detailed reference viewed: 30 (0 ULg)
Une prothèse discale!? Ca marche ?! C'est remboursé ?!
Scientific conference (2012, March 14)Detailed reference viewed: 70 (5 ULg)
Prothèse implantaire fixe scellée et vissée à l'heure du numérique
in Information Dentaire (L') (2011), 12Detailed reference viewed: 10 (1 ULg)
Prothèse implantaire fixe scellée et vissée à l'heure du numérique
in Information Dentaire (L') (2011), 12Detailed reference viewed: 32 (7 ULg)