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Peer Reviewed
See detailImplication of aggressive, submissive and reproductive sounds in the way of life of clownfishes
Colleye, Orphal ULiege; Parmentier, Eric ULiege

Conference (2012)

Clownfishes are brightly coloured coral reef fishes well known for their mutualistic symbiosis with tropical sea anemones. These fishes live in social groups in which there is a size-based dominance ... [more ▼]

Clownfishes are brightly coloured coral reef fishes well known for their mutualistic symbiosis with tropical sea anemones. These fishes live in social groups in which there is a size-based dominance hierarchy. In this structure where sex is socially controlled, agonistic interactions are numerous and serve to maintain size differences between individuals adjacent in rank. Clownfishes are also prolific callers whose sounds seem to play an important role in the social hierarchy. Agonistic interactions being involved in daily behaviour suggest how important acoustic communication might be in their way of life. Recording the different acoustic behaviours indicated that sounds are divided into two main categories: aggressive sounds produced in conjunction with threat postures (charge and chase), and submissive sounds always emitted when fish exhibited an appeasement display (namely the head shaking movements). Both types of sounds showed size-related intraspecific variation in dominant frequency and pulse duration: smaller individuals produce higher frequency and shorter duration pulses than larger ones. Consequently, these sonic features might be useful cues for individual recognition within the group. On the other hand, no acoustic call was associated with courtship and reproductive behaviours. Unlike other pomacentrids, sounds are not produced for mate attraction in clownfishes but to reach and to defend their breeding status, which explains why constraints are not important enough for promoting call diversification in this genus. However, acoustic signals seem to be an integral part of the peculiar way of life of clownfishes, although they are restricted to agonistic interactions only. [less ▲]

Detailed reference viewed: 109 (4 ULiège)
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See detailImplication of AurA kinase in GBM cells chemotaxis in response to the production of CXCL12 in the subventricular zones
Willems, Estelle ULiege; Dedobbeleer, Matthias ULiege; LOMBARD, Arnaud ULiege et al

Poster (2016, October 13)

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the ... [more ▼]

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the understanding of GBM recurrences has been the identification of GBM-initiating cells (GIC). GIC are thought to be deeply involved in GBM recurrences. Our lab designed a mouse model by grafting human GBM cells in the striatum. After the graft, we observed that tumors develop in the mouse striatum and that GIC specifically invade the subventricular zones (SVZ). SVZ are stem cells niches crucial for adult neurogenesis which seems particularly propitious for gliomagenesis since they are abundant in growth factors and permissive to proliferation. We therefore looked for soluble factors secreted by the SVZ environment and demonstrated that the local production of the CXCL12 chemokine in the SVZ is responsible for the GIC-directed migration. In this work, we aim to study the role GBM therapeutic resistance associated with the invasion of the SVZ. In this work, we identified a new actor of the CXCL12 pathway by the phosphoproteome analysis of U87MG cells stimulated with CXCL12: the mitotic kinase Aurora A (AurA) whose activity seems crucial for the CXCL12-dependent chemotaxis of GBM cells [less ▲]

Detailed reference viewed: 41 (5 ULiège)
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Peer Reviewed
See detailImplication of collagen type I-induced membrane-type 1-matrix metalloproteinase expression and matrix metalloproteinase-2 activation in the metastatic progression of breast carcinoma.
Gilles, Christine ULiege; Polette, M.; Seiki, M. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1997), 76(5), 651-60

We have previously demonstrated that fibroblasts and invasive human breast carcinoma (HBC) cells specifically activate matrix metalloproteinase-2 (MMP-2) when cultured on 3-dimensional gels of type I ... [more ▼]

We have previously demonstrated that fibroblasts and invasive human breast carcinoma (HBC) cells specifically activate matrix metalloproteinase-2 (MMP-2) when cultured on 3-dimensional gels of type I collagen but not a range of other substrates. We show here the constitutive expression of membrane-type 1 (MT1)-MMP in both fibroblasts, and invasive HBC cell lines, that have fibroblastic attributes presumably acquired through an epithelial-to-mesenchymal transition (EMT). Treatment with collagen type I increased the steady-state MT1-MMP mRNA levels in these cells but did not induce either MT1-MMP expression or MMP-2 activation in noninvasive breast carcinoma cell lines, which retain epithelial features. Basal MT3-MMP mRNA expression had a pattern similar to that of MT1-MMP but was not up-regulated by collagen. MT4-MMP mRNA was seen in both invasive and noninvasive HBC cell lines and was also not collagen-regulated, and MT2-MMP mRNA was not detected in any of the HBC cell lines tested. These data support a role for MT1-MMP in the collagen-induced MMP-2-activation seen in these cells. In situ hybridization analysis of archival breast cancer specimens revealed a close parallel in expression of both collagen type I and MT1-MMP mRNA in peritumoral fibroblasts, which was correlated with aggressiveness of the lesion. Relatively high levels of expression of both mRNA species were seen in fibroblasts close to invasive tumor nests and, although only focally, in certain areas close to preinvasive tumors. These foci may represent hot spots for local degradation and invasive progression. Collectively, these results implicate MT1-MMP in collagen-stimulated MMP-2 activation and suggest that this mechanism may be employed in vivo by both tumor-associated fibroblasts and EMT-derived carcinoma cells to facilitate increased invasion and/or metastasis. [less ▲]

Detailed reference viewed: 24 (4 ULiège)
See detailImplication of follicular dendritic cells in prion diseases
Defaweux, Valérie ULiege

Master of advanced studies dissertation (2002)

In prion diseases, FDCs seem to be the major sites of extraneuronal cellular prion protein (PrPc) expression and the principal sites of the infectious agent (PrPres) accumulation in lymph organs. Two ways ... [more ▼]

In prion diseases, FDCs seem to be the major sites of extraneuronal cellular prion protein (PrPc) expression and the principal sites of the infectious agent (PrPres) accumulation in lymph organs. Two ways of research has been considered: firstly, a new monoclonal antibody directed against bovine follicular dendritic cells (FDC-B1) developed in our laboratory has been characterised. We have shown that the antigen detected by FDC-B1 is expressed exclusively on FDCs surface of ruminants’ lymphoid organs. This protein seems to be a membrane glycoprotein of more or less 28 kDa whose sequence will be soon under determination. FDC-B1 will be a precious tool to detect FDC implication in scrapie infected sheep and in bovine spongiform affected cows. Secondly, the expression of PrPc isoforms has been analysed on bovine FDC depleted and enriched fractions and has been compared to bovine brain extracts. We demonstrated variation in the PrPc patterns of glycosylation between bovine FCD depleted and enriched cell populations. Moreover, glycosyl residues seemed to be different between immune and neuronal PrPc. As interaction of PrPc and PrPres appears to be a crucial pathogenic step promoted by homology, variation in PrPc glycoforms could explain the absence of infectivity in most bovine lymph organs affected by bovine spongiform encephalopathy. [less ▲]

Detailed reference viewed: 25 (4 ULiège)
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See detailImplication of HDAC-5 in heterochromatin replication
Peixoto, P; Castronovo, V; Matheus, N et al

Poster (2012)

Detailed reference viewed: 23 (5 ULiège)
Peer Reviewed
See detailImplication of honeydew microflora in ant-aphid mutualism
Fischer, Christophe ULiege; Lognay, Georges ULiege; Haubruge, Eric ULiege et al

Poster (2012, February 10)

Some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection. From a behavioral point of ... [more ▼]

Some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection. From a behavioral point of view, this phe-nomenon has been well studied from decades. However, its chemistry and semiochemical mechanisms are still largely unknown. This study aims to identify semiochemicals involved in the establishment of this relation, using both chemical and behavioral approaches. Bioassays revealed that the great-est part of ant attraction toward aphid colonies is due to honeydew volatile compounds; enabling ant scouts to find more quickly aphid colonies and distantly recognize myrmecophilous species. Many of those VOCs seeming to have microbial origins, the main honeydew microorganisms have been isolated and their roles in VOCs production and ant attraction have been investigated. It appeared that honeydew microflora holds a key role in the establishment of ant-aphids mutualistic relationship. [less ▲]

Detailed reference viewed: 125 (14 ULiège)
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See detailImplication of Importin-8 in mouse brain development
Nganou, Gerry ULiege; Coumans, Bernard ULiege; De Nijs, Laurence et al

Poster (2016, December 06)

Importin-8 (IPO8) is a protein that regulates the nucleocytoplasmic transport of some proteins important for cerebral development (Ago2, c-Jun, Smad4). In HEK cells, IPO8 seems to not plays others roles ... [more ▼]

Importin-8 (IPO8) is a protein that regulates the nucleocytoplasmic transport of some proteins important for cerebral development (Ago2, c-Jun, Smad4). In HEK cells, IPO8 seems to not plays others roles (like mitotic spindle formation, primary cilium transport). In situ hybridization performed in mouse brains slices shows that IPO8 is already expressed at E14. Moreover, inhibition of its action (IPO8) in embryonic mouse brain leads to impairment of neuroblast migration to upper cortical layers. IPO8 could then be at the origin of some pathologies with neuronal migration deficit. [less ▲]

Detailed reference viewed: 33 (9 ULiège)
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Peer Reviewed
See detailImplication of Importin-8 in mouse brain development
Nganou, Gerry ULiege; Denijs, Laurence; Lakaye, Bernard ULiege et al

Poster (2016, October 08)

Regulation of nucleocytoplasmic transport of proteins by the karyopherin superfamily is critical for cell physiology as it controls mainy fundamental processes such as division, differenciation, migration ... [more ▼]

Regulation of nucleocytoplasmic transport of proteins by the karyopherin superfamily is critical for cell physiology as it controls mainy fundamental processes such as division, differenciation, migration, adaptation to external environment etc. Beside this fundamental role, ß1 and ß2 members of this superfamily are also implicated in mitosis and ciliary entry respectively. Importin-8 (IPO8), a member of the β-karyopherin family, is reported to control the transport Ago-2, c-Jun and Smad-4 for example, three proteins important for brain development. First, we have verify the subcellular localisation of IPO8 in HEK and hTert cells. No colocalisation with either the mitotic spindle or the primary cilia could be observed. So it seems IPO8 only plays a role in nuclear transport of proteins. Then we have assessed the expression of IPO8 in mouse brain by In Situ Hybridization at various embryonic (E12, E14, E18) and post natal age (P5, P60). A strong expression was observed during embryonic stages, and especially in the ventricular zone and the cortical plate of the cerebral cortex and the ganglionic eminences both at E14. Therefore, the implication of IPO8 in the radial migration has been assessed by in utero electroporation of shRNA at E14. Three days after IUE, we observed that neuroblast accumulates in the Intermediate zone (IZ) and do not reach the cortical plate (CP) in constrast to the control condition. This effect can be corrected by coexpressing a form of IPO8 that is not targeted by the shRNA, demonstrating the specificity of the effect. In conclusion, regarding its role in transport, IPO8 could modulate neurons migration in the developing brain and could be also at the origin of some diseases associated with neurons migration defects. [less ▲]

Detailed reference viewed: 32 (8 ULiège)
See detailImplication of Methylglyoxal, a reactive dicarbonyl, in cellular senescence
Durieux, Florence ULiege

Poster (2015, December)

Detailed reference viewed: 8 (1 ULiège)
See detailImplication of Methylglyoxal, a reactive dicarbonyl, in cellular senescence
Durieux, Florence ULiege

Poster (2016, January)

Detailed reference viewed: 10 (2 ULiège)