Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailItraconazole corneofungimetry bioassay on Malassezia species.
Pierard, Gérald ULg; Arrese Estrada, Jorge ULg; Pierard, Claudine ULg

in Mycoses (2004), 47(9-10), 418-21

Yeasts of the genus Malassezia are part of the normal skin biocenosis and are involved in a series of distinct skin disorders and specific dermatomycoses in man and animals. Several species are currently ... [more ▼]

Yeasts of the genus Malassezia are part of the normal skin biocenosis and are involved in a series of distinct skin disorders and specific dermatomycoses in man and animals. Several species are currently distinguished. Their relative in vitro susceptibility to antifungals appears different according to the species and to the nature and route of administration of the drug. Corneofungimetry is an ex vivo bioassay allowing to test the fungal response on human stratum corneum following oral intake of a given antifungal by volunteers. Two series of cyanoacrylate skin surface strippings (CSSS) were harvested from the volar forearm of 30 volunteers before and after a 2-week treatment with itraconazole 200 mg daily. They were coated by olive oil and inoculated with suspensions of seven different Malassezia spp. After a 1-week culture on CSSS, the amount of viable yeasts was assessed using neutral red staining assisted by computerized image analysis. Growth of the seven species was not similar on the CSSS from untreated stratum corneum. The ranking order from the most proliferative to the least was M. restricta, M. sympodialis, M. globosa, M. furfur, M. obtusa, M. slooffiae and M. pachydermatis. Their growth was abated to almost the same level after itraconazole treatment. It is concluded that in vivo treatment with itraconazole is highly active against all Malassezia spp. colonizing the human stratum corneum. [less ▲]

Detailed reference viewed: 37 (2 ULg)
Full Text
Peer Reviewed
See detailiTRAQ-based analysis of changes in the cassava root proteome reveals pathways associated with post-harvest physiological deterioration.
Owiti, Judith; Grossmann, Jonas; Gehrig, Peter et al

in The Plant journal : for cell and molecular biology (2011), 67(1), 145-56

The short storage life of harvested cassava roots is an important constraint that limits the full potential of cassava as a commercial food crop in developing countries. We investigated the molecular ... [more ▼]

The short storage life of harvested cassava roots is an important constraint that limits the full potential of cassava as a commercial food crop in developing countries. We investigated the molecular changes during physiological deterioration of cassava root after harvesting using isobaric tags for relative and absolute quantification (iTRAQ) of proteins in soluble and non-soluble fractions prepared during a 96 h post-harvest time course. Combining bioinformatic approaches to reduce information redundancy for unsequenced or partially sequenced plant species, we established a comprehensive proteome map of the cassava root and identified quantitatively regulated proteins. Up-regulation of several key proteins confirmed that physiological deterioration of cassava root after harvesting is an active process, with 67 and 170 proteins, respectively, being up-regulated early and later after harvesting. This included regulated proteins that had not previously been associated with physiological deterioration after harvesting, such as linamarase, glutamic acid-rich protein, hydroxycinnamoyl transferase, glycine-rich RNA binding protein, beta-1,3-glucanase, pectin methylesterase, maturase K, dehydroascorbate reductase, allene oxide cyclase, and proteins involved in signal pathways. To confirm the regulation of these proteins, activity assays were performed for selected enzymes. Together, our results show that physiological deterioration after harvesting is a highly regulated complex process involving proteins that are potential candidates for biotechnology approaches to reduce such deterioration. [less ▲]

Detailed reference viewed: 30 (0 ULg)
Full Text
Peer Reviewed
See detailThe iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca
Romero, D.; de Vicente, A.; Rakotoaly, R. H. et al

in Molecular Plant-Microbe Interactions [=MPMI] (2007), 20(4), 430-440

Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on ... [more ▼]

Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopeptides have a major role in the antagonism of B. subtilis toward P fusca. [less ▲]

Detailed reference viewed: 60 (3 ULg)
Full Text
Peer Reviewed
See detailIUE and other new observations of the slow nova RR Tel
Penston, M. V.; Benvenuti, P.; Cassatella, A. et al

in Monthly Notices of the Royal Astronomical Society (1983), 202

IUE satellite UV spectra of RR Tel covering 1150-3200 A at high and low dispersion through both large and small apertures are reported, and a list of 431 lines is presented which gives measured wavelength ... [more ▼]

IUE satellite UV spectra of RR Tel covering 1150-3200 A at high and low dispersion through both large and small apertures are reported, and a list of 431 lines is presented which gives measured wavelength, intensity, and full width at half maximum. Over three-quarters of the lines are identified, and a correlation is noted between line width and ionization energy. The lines identified, which include common species ionized from one to four times, are generally resonance, forbidden or semiforbidden lines but also include the recombination lines for C, O and Ne. Many Fe II lines are present. Forbidden line wavelengths are used to define intersystem separations of energy levels in some species. The continuum energy distribution yielded by low dispersion data is not due to a simple recombination of gaseous emission processes and a hot star or accretion disk, but the very high ratio of the energy in the lines to that in the continuum of 2.4 indicates that a high temperature source must be present. [less ▲]

Detailed reference viewed: 20 (0 ULg)
See detailIUE and other new observations of the slow nova RR Tel.
Penston, M. V.; Benvenuti, P.; Cassatella, A. et al

in NASA Conference Publication (1981)

Detailed reference viewed: 9 (0 ULg)
Full Text
See detailIUFRO guidelines for designing multipurpose resource inventories: a project of IUFRO Research group 4.02.02 Vienna
Rondeux, Jacques ULg

in LUND, H. Gyde (Ed.) IUFRO Guidelines for designing multipurpose resource inventories: a project of IUFRO Research group 4.02.02 Vienna: IUFRO World Series (1998)

Detailed reference viewed: 27 (2 ULg)
Peer Reviewed
See detailIUG-/ZOUG- dans une inscription latine écrite en lettres grecques (CIJ I 215)
Rochette, Bruno ULg

in Zeitschrift für Papyrologie und Epigraphik (1997), 115

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailThe IUGS Devonian-Carboniferous Working Group: a report on activities, 1978-1984
Paproth, e; Streel, Maurice ULg

in Courier Forschungsinstitut Senckenberg (1984), 67

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailIUPAC World Polymer Congress – 40th International Symposium on Macromolecules
Cuenot, S.; Duwez, Anne-Sophie ULg; Daussin, R. et al

Conference (2004, July 04)

Detailed reference viewed: 11 (0 ULg)
See detailIUVS dayglow analysis
Jain; Evans; Stevens et al

Conference (2015, March)

Detailed reference viewed: 18 (0 ULg)
See detailIUVS observations of Nitric Oxide nightglow
Stiepen, Arnaud ULg; The IUVS team

Conference (2015, September)

Detailed reference viewed: 23 (4 ULg)
See detailIUVS periapse MUV observations overview
Stiepen, Arnaud ULg

Scientific conference (2015, October)

Detailed reference viewed: 13 (0 ULg)
See detailIUVS periapse observations of Mars’ Nitric Oxide
Stiepen, Arnaud ULg; IUVS team

Scientific conference (2015, June 24)

Detailed reference viewed: 20 (0 ULg)
Full Text
See detailIVA e autorità pubbliche : l’esperienza belga nel quadro del diritto europeo
Bourgeois, Marc ULg

Scientific conference (2016, May 05)

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailIVA. I soggetti passivi dell’imposta : La nozione di attività economica, i diversi tipi di attività economica e professionale
Bourgeois, Marc ULg; Greggi, Marco

in Di Pietro, A. (Ed.) Lo stato della fiscalità nell’Unione europea. L’esperienza e l’efficacia dell’armonizzazione (2003)

Detailed reference viewed: 34 (0 ULg)
Full Text
See detailIvoire des trois résurrections
Van den Bossche, Benoît ULg

in Jaffré, Géraldine; Marchant, Caroline (Eds.) Trésors classés en Fédération Wallonie-Bruxelles (2015)

Detailed reference viewed: 14 (0 ULg)
See detailLes ivoires liégeois
Van den Bossche, Benoît ULg

in Van den Bossche, Benoît (Ed.) L’Art mosan: Liège et son pays à l’époque romane (du XIe au XIIIe siècle) (2007)

Detailed reference viewed: 42 (7 ULg)
Full Text
See detailIX L'anaphrodisie
Mormont, Christian ULg

in X.. (Ed.) Traité de médecine générale. 11, Sexologie (1987)

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailIxodes ricinus Tick Lipocalins: Identification, Cloning, Phylogenetic Analysis and Biochemical Characterization
Beaufays, Jérôme ULg; Adam, Benoit; Decrem, Yves et al

in PLoS ONE (2008), 3(12), 3941

Background: During their blood meal, ticks secrete a wide variety of proteins that interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation of ... [more ▼]

Background: During their blood meal, ticks secrete a wide variety of proteins that interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation of the inflammatory response. Methodology/Principal Findings: Screening a cDNA library in association with RT-PCR and RACE methodologies allowed us to identify 14 new lipocalin genes in the salivary glands of the Ixodes ricinus hard tick. A computational in-depth structural analysis confirmed that LIRs belong to the lipocalin family. These proteins were called LIR for ``Lipocalin from I. ricinus'' and numbered from 1 to 14 (LIR1 to LIR14). According to their percentage identity/similarity, LIR proteins may be assigned to 6 distinct phylogenetic groups. The mature proteins have calculated pM and pI varying from 21.8 kDa to 37.2 kDa and from 4.45 to 9.57 respectively. In a western blot analysis, all recombinant LIRs appeared as a series of thin bands at 50-70 kDa, suggesting extensive glycosylation, which was experimentally confirmed by treatment with N-glycosidase F. In addition, the in vivo expression analysis of LIRs in I. ricinus, examined by RT-PCR, showed homogeneous expression profiles for certain phylogenetic groups and relatively heterogeneous profiles for other groups. Finally, we demonstrated that LIR6 codes for a protein that specifically binds leukotriene B4. Conclusions/Significance: This work confirms that, regarding their biochemical properties, expression profile, and sequence signature, lipocalins in Ixodes hard tick genus, and more specifically in the Ixodes ricinus species, are segregated into distinct phylogenetic groups suggesting potential distinct function. This was particularly demonstrated by the ability of LIR6 to scavenge leukotriene B4. The other LIRs did not bind any of the ligands tested, such as 5-hydroxytryptamine, ADP, norepinephrine, platelet activating factor, prostaglandins D2 and E2, and finally leukotrienes B4 and C4. [less ▲]

Detailed reference viewed: 33 (5 ULg)