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See detailInsights into the metagenomic approach : identification and characterization of cellulases involved in bacterial cellulose synthesis
Berlemont, Renaud ULg; Delsaute, Maud ULg; Galleni, Moreno ULg

Conference (2010, March 22)

the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family ... [more ▼]

the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 1C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process. [less ▲]

Detailed reference viewed: 39 (2 ULg)
See detailInsights into the pathogenic PLMVd-peach tree relationship
Parisi, Olivier ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg et al

in International Symposium on Crop Protection: Proceeding (2015)

Detailed reference viewed: 33 (3 ULg)
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See detailInsights into the plant defense mechanisms induced by Bacillus lipopeptides
Ongena, Marc ULg; Henry, G.; Adam, A. et al

in Biology of Plant-Microbe Interactions (2010)

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See detailInsights into the plant defense mechanisms induced by Bacillus lipopeptides.
Ongena, Marc ULg; Henry, Guillaume ULg; Adam, Akram et al

Poster (2009, July)

Detailed reference viewed: 60 (8 ULg)
See detailInsights into vaccine strategy to growth hormone
Renaville, Robert ULg; Maiter, Dominique; Deaver, Dan et al

Scientific conference (1998)

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See detailInsights on Molecular Mechanisms of Chondrocytes Death in Osteoarthritis
CHARLIER, Edith ULg; RELIC, Biserka ULg; Deroyer, Céline ULg et al

in International Journal of Molecular Sciences (2016)

Abstract: Osteoarthritis (OA) is a joint pathology characterized by progressive cartilage degradation. Medical care is mainly based on alleviating pain symptoms. Compelling studies report the presence of ... [more ▼]

Abstract: Osteoarthritis (OA) is a joint pathology characterized by progressive cartilage degradation. Medical care is mainly based on alleviating pain symptoms. Compelling studies report the presence of empty lacunae and hypocellularity in cartilage with aging and OA progression, suggesting that chondrocyte cell death occurs and participates to OA development. However, the relative contribution of apoptosis per se in OA pathogenesis appears complex to evaluate. Indeed, depending on technical approaches, OA stages, cartilage layers, animal models, as well as in vivo or in vitro experiments, the percentage of apoptosis and cell death types can vary. Apoptosis, chondroptosis, necrosis, and autophagic cell death are described in this review. The question of cell death causality in OA progression is also addressed, as well as the molecular pathways leading to cell death in response to the following inducers: Fas, Interleukin-1 (IL-1 ), Tumor Necrosis factor- (TNF- ), leptin, nitric oxide (NO) donors, and mechanical stresses. Furthermore, the protective role of autophagy in chondrocytes is highlighted, as well as its decline during OA progression, enhancing chondrocyte cell death; the transition being mainly controlled by HIF-1 /HIF-2 imbalance. Finally, we have considered whether interfering in chondrocyte apoptosis or promoting autophagy could constitute therapeutic strategies to impede OA progression. [less ▲]

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See detailInsights on the role played by temperature on the biocontrol agent Bacillus amyloliquefaciens strain S499
Puopolo, Gerardo; Jourdan, E.; Ongena, Marc ULg et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2013), 86

Detailed reference viewed: 13 (2 ULg)
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See detailInsights upon the adsorption mechanism of KAX on malachite
Kanda, Jean-Marie; Kongolo, Mukendi; Gaydardzhiev, Stoyan ULg et al

in Yianatos, Juan (Ed.) Proceedings of the XXVII International Mineral Processing Congress (2014, October 20)

The adsorption patterns of potassium amylxanthate on the surface of high purity malachite subjected to prior sulfidization with NaSH have been followed and elucidated. DRIFTS spectroscopy has been used to ... [more ▼]

The adsorption patterns of potassium amylxanthate on the surface of high purity malachite subjected to prior sulfidization with NaSH have been followed and elucidated. DRIFTS spectroscopy has been used to trace the associated physicochemical alterations as well as the chemical species present on the mineral surface. Varying the collector concentration from 5.10-4 to 1.10-2 mol/L has led to different degree of surface coverage of the alkyl groups in combination with formation of organometallic hydrophobic complexes of CuX and CuX – X2 type. The results reveal that the CuX complex formed on the malachite surface without sulfidizing agent is more unstable and prone to dissolution-precipitation phenomena. Based upon the constructed adsorption isotherms and upon the degree of statistical coverage, different zones of mineral-reagent interactions could be distinguished. They correspond respectively to the monolayer, bi-layer and tridimensional condensation domains. In each of the identified zones mono or double layer adsorption patterns of the collector are proposed leading respectively to formation of surface compounds with different degree of hydrophobicity. [less ▲]

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See detailInsitutional assimilation in the wake of EU competition law decentralisation
Van Cleynenbreugel, Pieter ULg

in Competition Law Review (2013), 8(3), 285-312

This article delves into the issue of institutional assimilation, i.e. the obligation, as a matter of EU law, for national authorities to adapt themselves to the needs imposed by an ever more streamlined ... [more ▼]

This article delves into the issue of institutional assimilation, i.e. the obligation, as a matter of EU law, for national authorities to adapt themselves to the needs imposed by an ever more streamlined EU law enforcement framework. Relying on the case law by the ECtHR, this paper outlines how particularly national competition authorities have had to adapt in this respect. [less ▲]

Detailed reference viewed: 11 (0 ULg)
See detailInsolvabilité et garanties
Georges, Frédéric ULg

Book published by Larcier (2014)

Detailed reference viewed: 13 (3 ULg)
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See detailInsolvabilité européenne et procédures secondaires
Wautelet, Patrick ULg

in Brulard, Yves (Ed.) Règlement 2015/848 relatif aux procédures d'insolvabilité - premier commentaire (2015)

Ce texte tente d'éclairer la portée des dispositions du Règlement N° 2015/848 du 20 mai 2015 relatives aux procédures secondaires : après un bilan sommaire de l'application des dispositions du Règlement ... [more ▼]

Ce texte tente d'éclairer la portée des dispositions du Règlement N° 2015/848 du 20 mai 2015 relatives aux procédures secondaires : après un bilan sommaire de l'application des dispositions du Règlement 1346/2000 relatives aux procédures secondaires, qui s'appuie principalement sur la jurisprudence de la Cour de justice, le texte commente les principales innovations du Règlement révisé. Une attention particulière est accordée à la technique de l'engagement par laquelle le syndic de la procédure principale peut éviter l'ouverture d'une procédure secondaire. [less ▲]

Detailed reference viewed: 156 (0 ULg)
See detailL'insoutenable légèreté de l'air : le cas de la gestion olfactive citoyenne de l'environnement
Melard, François ULg

Scientific conference (2012, November 19)

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See detail"Inspecter un corps mort" : contribution des rapports médicaux conservés sur papyrus (Ier – IVe siècles de notre ère) à l’histoire de la pratique médico-légale antique
Ricciardetto, Antonio ULg

in Charlier, Philippe; Gourevitch, Danielle (Eds.) 4e Colloque International de Pathographie (Saint Jean de Côle, 13-15 mai 2011) (2013, May)

Detailed reference viewed: 79 (33 ULg)
See detailL'inspecteur Convers et les intégristes
Mouton, Charles ULg

Book published by Persée (2010)

Detailed reference viewed: 26 (3 ULg)