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See detailMeasurement of proteoglycans, elastase, collagenase and protein in synovial fluid in inflammatory and degenerative arthropathies
Gysen, Ph; Malaise, Michel ULg; Gaspar, S. et al

in Clinical Rheumatology (1985), 4(1), 39-50

The number of leucocytes and the concentrations of protein, proteoglycans (PG), elastase a1 proteinase inhibitor complexes and collagenolytic activity were measured in the synovial fluid (SF) of 15 ... [more ▼]

The number of leucocytes and the concentrations of protein, proteoglycans (PG), elastase a1 proteinase inhibitor complexes and collagenolytic activity were measured in the synovial fluid (SF) of 15 patients with rheumatoid arthritis (RA) and 18 with osteoarthritis (OA). The mean levels of protein and collagenase and the number of leucocytes were higher in RA than in OA SF. However, the mean level of PG was higher of OA SF than in RA. In the latter, they were principally in the form of monomers and fragments while in the former they were in the form of aggregates and monomers. There was a direct relationship between the concentration of E-a/Pi and either the number of white cells or the concentration of synovial proteins, suggesting that the measurement of E-a/Pi complexes is a biochemical index of the local inflammatory reaction. There was an inverse correlation between the concentrations ofPG and E-a/Pi which may reflect the effect of degradation in PG of elastase and other enzymes released at the same time. Finally, there was a direct relationship between the concentration of E-a/Pi and collagenase which may be the reflection of a simultaneous release of various enzymes from leucocytes and macrophages. [less ▲]

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See detailMeasurement of pulmonary inertance in Equids
Art, Tatiana ULg; Lekeux, Pierre ULg; Gustin, Pascal ULg et al

in European Respiratory Journal (1989), 2

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See detailMeasurement of quality of life in cardiac rehabilitation
Dupuis, Gilles; Taillefer, Marie Christine; Etienne, Anne-Marie ULg et al

in Jobin, Jean; Maltais, François; LeBlanc, Pierre (Eds.) et al Advances in Cardiopulmonary rehabilitation (2000)

Detailed reference viewed: 93 (6 ULg)
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See detailMeasurement of radiative lifetimes and determination of transition probabilities of astrophysical interest in HoIII
Biémont, Emile ULg; Palmeri, P.; Quinet, Pascal ULg et al

in Monthly Notices of the Royal Astronomical Society (2001), 328(4), 1085-1090

Lifetimes of six levels belonging to the 4f(10)6p configuration of Ho III have been measured for the first time using the time-resolved laser-induced fluorescence method. They are compared with ... [more ▼]

Lifetimes of six levels belonging to the 4f(10)6p configuration of Ho III have been measured for the first time using the time-resolved laser-induced fluorescence method. They are compared with multiconfigurational pseudo-relativistic Hartree-Fock calculations. A very good agreement is found. Using the experimental lifetimes and theoretical branching fractions, a first set of transition probabilities of astrophysical interest has been obtained for this ion. [less ▲]

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See detailMeasurement of respiratory function by impulse oscillometry in horses
Van Erck, Emmanuelle; Votion, Dominique ULg; Art, Tatiana ULg et al

in Equine Veterinary Journal (2004), 36(1), 21-28

Reasons for performing study: Due to technical implementations and lack of sensitivity, pulmonary function tests are seldom used in clinical practice. Impulse oscillometry (IOS) could represent an ... [more ▼]

Reasons for performing study: Due to technical implementations and lack of sensitivity, pulmonary function tests are seldom used in clinical practice. Impulse oscillometry (IOS) could represent an alternative method. Objectives: To define feasibility, methodology and repeatability of IOS, a forced oscillation technique that measures respiratory resistance (R-rs) and reactance (X-rs) from 5 to 35 Hz during spontaneous breathing, in horses. Methods: Using 38 healthy horses, R-rs and X-rs reference values were defined and influence of individual biometrical parameters was investigated. In addition, IOS measurements of 6 horses showing clinical signs of heaves were compared to those of 6 healthy horses. Results: Airtightness and minimal dead space in the facemask were prerequisites to IOS testing and standardisation of head position was necessary to avoid variations in R-rs due to modified upper airway geometry. In both healthy and diseased animals, measurements were repeatable. In standard-type breeds, the influence of the horse's size on IOS parameters was negligible. An increase in R-5Hz greater than 0.10 kPa/l/sec and R-5Hz>R-10Hz, combined with negative values of X-rs between 5 and 20 Hz, was indicative of heaves crisis. Conclusions: IOS is a quick, minimally invasive and informative method for pulmonary function testing in healthy and diseased horses. Potential relevance: IOS is a promising method for routine and/or field respiratory clinical testing in the equine species. [less ▲]

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See detailMeasurement of respiratory impedance in healthy calves using the impulse oscillation technique - physiological and methodological aspects
Reinhold, Petra; Smith, H.-J.; Langenberg, Annelie et al

in Veterinary Journal (1998), 155(1), 27-38

The aim of the present study was to investigate methodological and physiological aspects of complex respiratory impedance measurements in calves using the impulse oscillation technique. To apply the ... [more ▼]

The aim of the present study was to investigate methodological and physiological aspects of complex respiratory impedance measurements in calves using the impulse oscillation technique. To apply the system to animals, a tightly fitting face mask was used. The measuring system was modified by placing a flexible tube between the pneumotachograph and the loudspeaker. Complex respiratory impedance measurements were not significantly influenced by the modification. Linear correlations were found between body weight and respiratory impedance; as body weight increased, resistance decreased and reactance increased. The methodological variability of impedance measurements using the impulse technique was found to be extremely small. Diurnal variations of respiratory impedance were considered to be the main source of intra-individual variability. When comparing different animals (even when healthy and matched for age and body weight) our results suggest that inter-individual biological variability must be carefully considered [less ▲]

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See detailMeasurement of ribosomal RNA turnover in vivo by use of deuterium-labeled glucose.
Defoiche, Julien; Zhang, Yan; Lagneaux, Laurence et al

in Clinical Chemistry (2009), 55(10), 1824-33

BACKGROUND: Most methods for estimation of rates of RNA production are not applicable in human in vivo clinical studies. We describe here an approach for measuring ribosomal RNA turnover in vivo using [6 ... [more ▼]

BACKGROUND: Most methods for estimation of rates of RNA production are not applicable in human in vivo clinical studies. We describe here an approach for measuring ribosomal RNA turnover in vivo using [6,6-(2)H(2)]-glucose as a precursor for de novo RNA synthesis. Because this method involves neither radioactivity nor toxic metabolites, it is suitable for human studies. METHODS: For method development in vitro, a lymphocyte cell line (PM1) was cultured in the presence of [6,6-(2)H(2)]-glucose. RNA was extracted, hydrolyzed enzymatically to ribonucleosides, and derivatized to either the aldonitrile tetra-acetate or the pentafluoro triacetate derivative of the pentose before GC-MS. We identified optimum derivatization and analysis conditions and demonstrated quantitative incorporation of deuterium from glucose into RNA of dividing cells. RESULTS: Pilot clinical studies demonstrated the applicability of this approach to blood leukocytes and solid tissues. A patient with chronic lymphocytic leukemia received [6,6-(2)H(2)]-glucose (1 g/kg) orally in aliquots administered every 30 min for a period of 10 h. When we analyzed CD3(-) B cells that had been purified by gradient centrifugation and magnetic-bead adhesion, we observed deuterium enrichment, a finding consistent with a ribosomal RNA production rate of about 7%/day, despite the slow division rates observed in concurrent DNA-labeling analysis. Similarly, in 2 patients with malignant infiltration of lymph nodes, administration of [6,6-(2)H(2)]-glucose (by intravenous infusion for 24 h) before excision biopsy allowed estimation of DNA and RNA turnover in lymph node samples. CONCLUSIONS: Our study results demonstrate the proof-of-principle that deuterium-labeled glucose may be used to analyze RNA turnover, in addition to DNA production/cell proliferation, in clinical samples. [less ▲]

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See detailMeasurement of Right Ventricular Ejection Fraction and Volume by the Thermodilution Technique in the Unsedated Calf
Amory, Hélène ULg; Mc Entee, Kathleen ULg; Linden, Annick ULg et al

in Research in Veterinary Science (1994), 56(1), 75-81

This study was designed to test the applicability and the reproducibility of the thermodilution method in the measurement of right ventricular ejection fraction (EF), end-diastolic volume (EDV) and end ... [more ▼]

This study was designed to test the applicability and the reproducibility of the thermodilution method in the measurement of right ventricular ejection fraction (EF), end-diastolic volume (EDV) and end-systolic volume (ESV) in unsedated cattle. In the 16 healthy calves studied, it was possible, using a fast response thermodilution catheter, to obtain characteristic exponential steplike washout curves, allowing the calculation of right ventricular EF, EDV and ESV. Five to 10 successive thermodilution curves were recorded within a few minutes and two to five similar sets of measurements were performed throughout the same day to test the reproducibility of the technique. The same protocol was repeated the following day. Right ventricular EF, EDV and ESV were reproducible successively, throughout a given day and on following days. The mean intraset, intraday and interdays coefficients of variation ranged from a mean of 4.0 (SD 4.1) per cent to a mean of 18.2 (SD 7.9) per cent. Values of right ventricular volumes and EF agreed with those previously reported in various animal species. Thermodilution was, therefore, shown to be a useful method for measuring with a satisfactory reproducibility, right ventricular EF, EDV and ESV in the unsedated healthy calf [less ▲]

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See detailMeasurement of selected halogenated contaminants in human serum and milk using GCxGC-IDTOFMS
Focant, Jean-François ULg; sjodin, Andreas; Turner, Wayman et al

in Organohalogen Compounds (2004)

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See detailMeasurement of selected polybrominated diphenyl ethers, polybrominated and polychlorinated biphenyls, and organochlorine pesticides in human serum and milk using comprehensive two-dimensional gas chromatography isotope dilution time-of-flight mass spectrometry
Focant, Jean-François ULg; Sjodin, Andreas; Turner, Wayman E. et al

in Analytical Chemistry (2004), 76(21), 6313-6320

A new method using comprehensive two-dimensional gas chromatography and isotope dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) for the simultaneous measurement of selected polychlorinated ... [more ▼]

A new method using comprehensive two-dimensional gas chromatography and isotope dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) for the simultaneous measurement of selected polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and brominated flame retardants is presented. In contrast to the reference methods based on classical GC/MS, a single injection of the extract containing all compounds of interest results in accurate identification and quantification. Using GC x GC ensures the chromatographic separation of most compounds, and TOFMS allows mass spectral deconvolution of coeluting compounds as well as the use of C-13-labeled internal standards for quantification. Isotope ratio measurements of the most intense ions for both native and labels ensure the required specificity. The use of this new method with an automated sample preparation procedure developed at the Centers for Disease Control and Prevention (CDC) for the analysis of human serum and milk compared favorably to conventional isotope-dilution one-dimensional gas chromatography-high-resolution mass spectrometty (GC-IDHRMS) for the different human serum and milk pools tested. The instrumental detection limits ranged between 0.5 pg/muL and 10 pg/muL and the method detection limits ranged between I and 15 pg/muL (N = 59 analytes). The reproducibility of the method was almost as good as with GC-IDHRMS, the relative standard deviations ranging between 1 and 11% for OCPs measured in human serum. OCP, PBDE, and PCB levels measured using the two methods were highly correlated, and the deviations between the two methods were below 20% for most analytes with concentrations above 1 ng/g milk lipids. [less ▲]

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See detailMeasurement of serum albumin by protein electrophoresis and bromocresol green methods in canine and equine patients
Ramery, Eve ULg; Bureau, Fabrice ULg

in Veterinary Clinical Pathology (2012, December), 41(4), 5

Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported ... [more ▼]

Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported. However, in human medicine, at low albumin and high globulin concentrations, BCG also binds to α- and β-globulins fractions. Surprisingly, there is no recent literature evaluating the BCG method in diseased domestic animals. Objective: The purpose of the present study was therefore to compare the serum albumin values obtained by SPE and BCG in canine and equine patients. Methods: Albumin measured by BCG and SPE was compared in the serum from 30 canine and 30 equine patients. Results: Good correlation was found (Spearman´s rho rs = 0.91 in dogs and 0.84 in horses). However, Altman and Bland analysis of results showed a positive bias of BCG with SPE (3.41 g/l in horses and 0.41 g/l in dogs) that increased when only hypoalbuminemic patients were considered (4.17 g/l in horses and 1.31 g/l in dogs). Conclusion: Results confirm that, despite good correlation, BCG method tends to overestimate albumin, especially in hypoalbuminemic patients. This is particularly evident in horses. [less ▲]

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See detailMeasurement of shrinkage and cracks associated to convective drying of soft materials by X-ray microtomography
Léonard, Angélique ULg; Blacher, Silvia ULg; Marchot, Pierre ULg et al

in Drying Technology (2004), 22(7), 1695-1708

Traditionally, the measurement of shrinkage occurring during drying is performed by destructive or poorly accurate techniques such as volume displacement methods. Cracks detection and quantification are ... [more ▼]

Traditionally, the measurement of shrinkage occurring during drying is performed by destructive or poorly accurate techniques such as volume displacement methods. Cracks detection and quantification are realised either by destructive techniques or sophisticated but expensive nondestructive ones (NMR imaging). X-ray microtomography in combination with image analysis provides an accurate, nondestructive and easy to use technique to determine simultaneously shrinkage and crack extent. Results reported in this article concern drying of wastewater sludges whose management will become a real challenge in the years to come. These results show a clear relation between drying kinetics and crack development. This could be related to the development of internal diffusional limitations inducing moisture gradients and mechanical stresses leading to cracks formation. [less ▲]

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See detailMeasurement of soil surfaces profiles with an optical displacement transducer.
Destain, Marie-France ULg; Verbrugge, Jean-Claude ULg

in Proceedings, 9th International Conference of the International Society for Terrain Vehicle Systems, held in Barcelona, Spain, 31 August-4September 1987. Volume 1. (1987), 1

Microrelief of seedbeds after two different soil tillages were measured with an optical displacement transducer. Measurements were made after sowing and on two successive dates. A complete statistical ... [more ▼]

Microrelief of seedbeds after two different soil tillages were measured with an optical displacement transducer. Measurements were made after sowing and on two successive dates. A complete statistical analysis was obtained by means of power spectra and autocorrelation functions. The authors suggest using the absolute value of the slope of the tangent at the origin of the autocorrelation function with the aim of analysing the behaviour of small particles in the scope of soil degradation [less ▲]

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See detailMeasurement of the 2s2p2(4P)3s 3P2 lifetime in N II by beam-foil-laser spectroscopy
Bastin, Thierry ULg; Baudinet-Robinet, Y.; Garnir, Henri-Pierre ULg et al

in Journal Physical II (1993), 3

An accurate value for the radiative lifetime of the 2s2p2(4P)3s3P2 level in N II has been obtained using the beam-foil-laser spectroscopy method. The result (T = 0.456 ± 0.020 ns ) is shorter than the ... [more ▼]

An accurate value for the radiative lifetime of the 2s2p2(4P)3s3P2 level in N II has been obtained using the beam-foil-laser spectroscopy method. The result (T = 0.456 ± 0.020 ns ) is shorter than the beam-foil lifetime values reported previously for the 3s' 3P term. No theoretical value for the lifetime of this term is available at the present time. [less ▲]

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See detailMeasurement of the 3s' 3p2 lifetime in N II by beam-foil-laser spectroscopy
Baudinet-Robinet, Y.; Bastin, Thierry ULg; Dumont, Paul-Dominique ULg et al

Poster (1993)

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See detailMeasurement of the estrogenic activity of migration products from plastic contact materials
Simon, Coraline ULg; Onghena, M.; Covaci, A. et al

Poster (2013, October 19)

Baby bottles in plastic are widely used to feed newborns and infants. The polymers used for the manufacture of baby bottles are mainly polypropylene (PP), silicone, polyamide (PA) and polyethersulphone ... [more ▼]

Baby bottles in plastic are widely used to feed newborns and infants. The polymers used for the manufacture of baby bottles are mainly polypropylene (PP), silicone, polyamide (PA) and polyethersulphone (PES), since the ban of the use of bisphenol A (BPA), which exhibited estrogenic activity, in polycarbonate by European commission, in March 2011. For the plastic substitutes, Chemical substances, such as monomers or additives (antioxidants, plasticizers, clarifiers, etc.), may migrate from plastic baby bottle into its contents. This monomers and additives may exhibit at low concentrations estrogenic activity (EA), who can produce adverse effects, especially in newborn and infants. A recent study of Simoneau and al. give an overview of the amount of chemical substances which migrate from plastic baby bottle into its contents. The objective of this study is to measure EA of these substances. The study focus first on screening Human estrogen receptor agonistic and antagonistic activities of pure compounds identifed by Simoneau and al. using a cell based transactivational assay named “Chemically Activated LUciferase gene eXpression” (CALUX). Preliminary test with 3 Bisphenols (BPs) have been performed: BPA, Bisphenol S (BPS) and Bisphenol F (BPF). The dose–response curves obtained for BPA, BPS and BPF were compared with that of 17β-estradiol (E2). An agonistic effect was observed with the 3 BPs. BPS and BPF exhibited oestrogen-like response lower than that of BPA. [less ▲]

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See detailMeasurement of the force occuring at the impact of a non-lethal projectile with the Flexiforce sensor
Robbe, Cyril ULg; Nsiampa Ndompetelo, ULg; Papy, Alexandre

in 6th European Symposium on Non-Lethal Weapons 2011 proceedings (2011, May 16)

Force measurements are important to experimentally assess the effects of a non- lethal projectile on a human body. In addition they are also necessary to evaluate the bio-fidelity of a system regarding ... [more ▼]

Force measurements are important to experimentally assess the effects of a non- lethal projectile on a human body. In addition they are also necessary to evaluate the bio-fidelity of a system regarding PMHS (post mortem human subject) data. So far, as we can see in the literature, these measurements are usually achieved with an accelerometer placed inside the projectile. By assuming this projectile is perfectly rigid, the impact force can be calculated [1, 2]. However, the limitations are obvious: on the one hand the embedded accelerometer will change the projectile properties. On the other hand the force cannot directly be deduced for the deformable projectiles usually used nowadays. Therefore, a new system to measure impact force has to be developed. In this study, we propose to use the Flexiforce sensor, which is a cheap and very thin force sensor compatible with the expected measurement range [3]. The final objective is to realise an efficient force measurement on any kinetic energy non-lethal weapon (KENLW) projectile, using this particular sensor. We can find in the literature some static calibration [3,4]. As our application is highly dynamical, we started this study with the design and application of a dynamic calibration process. Then results from rigid projectiles (comparing to a classic KENLW projectile) impacting rigid targets (comparing to human body or a biofidelic surrogate) are collected and Flexiforce and accelerometer signals are compared (“rigid-rigid impacts). Finally, force measurements of projectiles impacting deformable surfaces (comparing to the projectile ) with the Flexiforce sensor are achieved (“rigid-soft impacts”). [less ▲]

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See detailMeasurement of the magnetic field inside the holes of a drilled bulk high-Tc superconductor
Lousberg, Grégory ULg; Fagnard, Jean-François ULg; Noudem, Jacques G et al

in Superconductor Science and Technology (2009), 22

We use macroscopic holes drilled in a bulk YBCO superconductor to probe its magnetic properties in the volume of the sample. The sample is subjected to an AC magnetic flux with a density ranging from 30 ... [more ▼]

We use macroscopic holes drilled in a bulk YBCO superconductor to probe its magnetic properties in the volume of the sample. The sample is subjected to an AC magnetic flux with a density ranging from 30 to 130 mT and the flux in the superconductor is probed by miniature coils inserted in the holes. In a given hole, three different penetration regimes can be observed: (i) the shielded regime, where no magnetic flux threads the hole; (ii) the gradual penetration regime, where the waveform of the magnetic field has a clipped sine shape whose fundamental component scales with the applied field; and (iii) the flux concentration regime, where the waveform of the magnetic field is nearly a sine wave, with an amplitude exceeding that of the applied field by up to a factor of two. The distribution of the penetration regimes in the holes is compared with that of the magnetic flux density at the top and bottom surfaces of the sample, and is interpreted with the help of optical polarized light micrographs of these surfaces. We show that the measurement of the magnetic field inside the holes can be used as a local characterization of the bulk magnetic properties of the sample. [less ▲]

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See detailMeasurement of the magnetic flux threading holes of drilled superconductors
Lousberg, Grégory ULg

Poster (2007, September)

Article associé : Measurement of the magnetic field inside the holes of a drilled bulk high-Tc superconductor

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