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See detailAscorbate potentiates the cytotoxicity of menadione leading to an oxidative stress that kills cancer cells by a non-apoptotic caspase-3 independent form of cell death
Verrax, Julien; Cadrobbi, Julie; Marques, Carole et al

in Apoptosis : An International Journal on Programmed Cell Death (2004), 9(2), 223-233

Hepatocarcinoma cells (TLT) were incubated in the presence of ascorbate and menadione, either alone or in combination. Cell death was only observed when such compounds were added simultaneously, most ... [more ▼]

Hepatocarcinoma cells (TLT) were incubated in the presence of ascorbate and menadione, either alone or in combination. Cell death was only observed when such compounds were added simultaneously, most probably due to hydrogen peroxide (H2O2) generated by ascorbate-driven menadione redox cycling. TLT cells were particularly sensitive to such an oxidative stress due to its poor antioxidant status. DNA strand breaks were induced by this association but this process did not correspond to oligosomal DNA fragmentation (a hallmark of cell death by apoptosis). Neither caspase-3-like DEVDase activity, nor processing of procaspase-3 and cleavage of poly(ADP-ribose) polymerase (PARP) were observed in the presence of ascorbate and menadione. Cell death induced by such an association was actively dependent on protein phosphorylation since it was totally prevented by preincubating cells with sodium orthovanadate, a tyrosine phosphatase inhibitor. Finally, while H2O2, when administered as a bolus, strongly enhances a constitutive basal NF-kappaB activity in TLT cells, their incubation in the presence of ascorbate and menadione results in a total abolition of such a constitutive activity. [less ▲]

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See detailAscorbate-induced fibroblast cell matrix: reaction of antibodies to procollagen I and III and fibronectin in an axial periodic fashion.
Furcht, L. T.; Wendelschafer-Crabb, G.; Mosher, D. F. et al

in Progress in Clinical & Biological Research (1980), 41

Fibronectin and procollagen types I and III are constituents of the extracellular matrix of human fibroblasts. Ultrastructural immunocytochemistry using the peroxidase anti-peroxidase method showed ... [more ▼]

Fibronectin and procollagen types I and III are constituents of the extracellular matrix of human fibroblasts. Ultrastructural immunocytochemistry using the peroxidase anti-peroxidase method showed fibronectin and procollagen antibodies reacting in continuous fashion on 10 nm diameter extracellular fibrils on human fibroblasts. Intracellular localization showed an intense accumulation of procollagen within cells cultured under routine conditions. This accumulation appeared almost as if there were a blockade in secretion of procollagen under routine culture conditions. Cells treated with ascorbic acid do not have the dense intracellular accumulation of procollagens seen with the apparent blockade of secretion in cells cultured under routine conditions. Ascorbate treated cells also have a more pronounced extracellular accumulation of matrix fibronectin and procollagen constituents. At the electromicroscopic level a new 40 nm diameter fibril is formed after ascorbic acid treatment of human fibroblasts. Antibody to fibronectin and procollagen I and III are seen binding to the 40 nm diameter fibrils in a periodic or stuttered appearance. The fibronectin and procollagen antibodies react with a 70 nm axial repeat along these 40 nm fibrils formed after ascorbate treatment. These studies suggest that under routine culture conditions "precursor" fibrils of fibronectin and procollagen are formed. Ascorbic acid treatment leads to enhanced matrix formation. Ultrastructural studies clearly show antibodies to fibronectin bind to fibronectin on native collagen fibrils formed by human fibroblasts cultured with ascrobic acid. Lastly there is an asymmetric or 70 nm axial periodic distribution of fibronectin along these definitive or mature collagen fibrils formed after ascorbic acid treatment. [less ▲]

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See detailAscorbic acid, Phenolic acid, Flavonoid and Carotenoid profiles of selected extracts from Ribes nigrum
Tabart, Jessica ULg; Kevers, Claire ULg; Evers, Danièle et al

in Journal of Agricultural and Food Chemistry (2011), 59

Small fruits such as berries have low energy contents, but high content of vitamins, micronutrients, and dietary fibres and constitute a good source of natural antioxidant compounds that are important ... [more ▼]

Small fruits such as berries have low energy contents, but high content of vitamins, micronutrients, and dietary fibres and constitute a good source of natural antioxidant compounds that are important constituents of the human diet. This study allowed to identify a large number of compounds in an extract of blackcurrant showing high antioxidant activity and to compare their profile in various parts of the plants (leaves, buds and berries). If it was known that berries contained very high levels of natural phenolic compounds, here we showed that leaves and buds could also be considered as a good sources of natural antioxidants. Indeed, they contained high amount of ascorbic acid, phenolic acids, flavonoids and carotenoids. An acetone mixture can extract several classes of phenolic compounds with a good yield of ascorbic acid, flavonols, flavan-3-ols and anthocyanins. For phenolic acids, it extracted fewer compounds than a specific extraction solution. [less ▲]

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See detailAseptic and automatable vitrification of human embryonic stem cells using defined media
Ectors, Fabien ULg

Conference (2012, October 22)

Aseptic and automatable vitrification of human embryonic stem cells using defined media Efficient recovery of biologically safe and undifferentiated hESCs is a major challenge after cryobanking ... [more ▼]

Aseptic and automatable vitrification of human embryonic stem cells using defined media Efficient recovery of biologically safe and undifferentiated hESCs is a major challenge after cryobanking, especially when their use for human therapy is foreseen. One drawback of actual cryopreservation protocols is the direct contact of the cells with liquid nitrogen, therefore cells can be in contact with heavy metals, bacteria, viruses or fungi during cooling and storage. We describe a novel cryopreservation method which uses aseptic vitrification1 (no direct contact with LN2) and only chemically defined materials and media, and is automatable. 1: Vitrification is a cryopreservation method based on the conversion of a liquid into a glass-like state by an infinite enhancement of its viscosity and without formation of ice crystals. [less ▲]

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See detailAseptic vitrification of blastocysts from infertile patients, egg donors and after IVM
Vanderzwalmen, Pierre ULg; Ectors, Fabien ULg; Grobet, Luc ULg et al

in Reproductive Biomedicine Online (2009), 19(5), 700-707

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See detailThe Asian Crisis Exchange Risk Exposure of U.S. Multinationals
Muller, Aline ULg; Verschoor, Willem

in Managerial Finance (2007), 33(9), 710-740

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See detailAsian Foreign Exchange Risk Exposure
Muller, Aline ULg; Verschoor, Willem

in Journal of the Japanese & International Economies (2007), 21(1), 16-37

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See detailASimplified Analytical Method for Estimating the Crushing Resistance of an Inclined Ship Side
Buldgen, Loïc ULg; Le Sourne, Hervé; Rigo, Philippe ULg

in Marine Structures (2013)

This paper provides a new contribution to the simplified analytical treatment of collisions between two ships. It is directly connected to the well-known super-elements method, which is a simplified ... [more ▼]

This paper provides a new contribution to the simplified analytical treatment of collisions between two ships. It is directly connected to the well-known super-elements method, which is a simplified procedure allowing for a quick estimation of the damages caused to both the striking and struck vessels during such events. In this article, a new analytical formulation is presented for estimating the impact resistance provided by inclined ship side panels. Two different scenarios are treated. We first deal with the case of an impact between the oblique plate and the stem of the striking ship, and then we consider the situation where the inclined panel is impacted by the bulb. For these two scenarios, an analytical formulation relating the force and the penetration is provided and these developments are validated by comparing them to the results of finite elements simulations. Finally, the new inclined plate super-element is integrated in a simplified model of a frigate collided by another ship, and the resistance given by the superelements method is then compared to the one obtained by a numerical simulation of this collision. [less ▲]

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See detail'Ask me Again in Twenty Years' Time'. A Conversation
Spinoy, Erik ULg

in Spinoy, Erik (Ed.) Loud & Bright Purple Birds & Blue Eyed Bitches Dancing (2009)

This is an interview with the young Belgian artist Tamara Van San, probing into her views of art and into her way of working and discussing concrete examples of her work.

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See detailAsk the Periphery – a vantage point for understanding city and nationalism relations
Farah, Jihad ULg; Teller, Jacques ULg

Conference (2012, February)

Studies on nationalism were often interested with the city, especially the city centers. The politics of heritage, the architecture of public buildings and the design and use of central public spaces are ... [more ▼]

Studies on nationalism were often interested with the city, especially the city centers. The politics of heritage, the architecture of public buildings and the design and use of central public spaces are often studied in relation to national identity politics. Municipal politics of the historical city in the face of central authorities’ urban politics is also frequently approached in the light of Nation-State building issues. If no doubt the consideration of city centers as a vantage point for understanding the relation between nationalism and the city is an interesting one that bore many fruits, we believe that a vantage point concentrating on the peripheries can highly contribute in unveiling other aspects of this relation. This paper aims at investigating the potentialities of this contribution. As the place where urbanization is taking place, the periphery is: - Where the national identity construction logic is put to test. First because, historically, it is here where the confrontation between the urban and the rural did first take place. Second, because rural exodus and migration, usually more important in the periphery, will continue to challenge it. - Where the city and the hinterland get connected through different national territory building networks. - Where the Nation-State central authorities will find the largest land reserves to develop their large emblematic projects. - Where urban fragmentation poses the strongest challenge to the unitarian message of nationalism, but also is where some nationalist movements might best develop In fact, the periphery represents equally a fertile ground for nationalism and for resistances to it. This paper will rely on the city of Beirut as a case study. [less ▲]

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See detailAsociación de adhesina F17 con producción de toxinas Shiga y otros factores de virulencia en Escherichia coli de origen bovino
Rodriguez, Sabrina ULg; Mercado, E.C.; Gioffré, A. et al

Poster (2001, October 07)

ASOCIACIÓN DE ADHESINA F17 CON PRODUCCIÓN DE TOXINAS SHIGA Y OTROS FACTORES DE VIRULENCIA EN Escherichia coli DE ORIGEN BOVINO Rodríguez S1; Mercado E1; Gioffré, A2; Cataldi A2; Elizondo A1; Rossetti C2 ... [more ▼]

ASOCIACIÓN DE ADHESINA F17 CON PRODUCCIÓN DE TOXINAS SHIGA Y OTROS FACTORES DE VIRULENCIA EN Escherichia coli DE ORIGEN BOVINO Rodríguez S1; Mercado E1; Gioffré, A2; Cataldi A2; Elizondo A1; Rossetti C2 (1) Instituto de Patobiología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. (2) Instituto de Biotecnología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. La adhesina F17 presenta variantes serológicas (F17a,F17b,F17c,F17d) que son expresadas por E.coli patógenos. Estos subtipos han sido detectados en bovinos con diarrea o septicemia y en ovinos septicémicos en países del hemisferio norte. F17 es altamente homóloga a fimbrias de P. mirabilis uropatógeno y H. influenzae biogr.aegyptius. En los bovinos, la expresión de F17 se ha asociado con varios factores de virulencia. En este trabajo se demuestra la presencia de fimbrias de la familia F17 en E.coli productoras de toxinas Shiga (Stx). Las cepas estudiadas provinieron de un brote de diarrea en un establecimiento de cría que afectó a 170 de un total de 600 terneros de 30-45 días. La detección de los subtipos de F17 se realizó por agluti-nación en lámina e immunodot y se confirmó por SDS-PAGE y Western blot. Las fimbrias F5 y F41 se investigaron por aglutinación en lámina. El antígeno de superficie CS31A se detectó por immunodot, confirmándose por SDS-PAGE y Western blot. Los genes que codifican para Stx (stx1, stx2) e intimina (eaeA) se detectaron por PCR, confirmándose la producción de Stx en células Vero. La producción de enterotoxina (StaP) se ensayó en ratón lactante. La serotipificación se realizó por la técnica de aglutinación. La detección de enterohemolisina (ehly) fue realizada en agar con 5% de eritrocitos lavados. Se comprobó la capacidad de colonización intestinal de las cepas F17+ por la técnica de adherencia in vitro a villi de ternero. En 7 de 9 animales estudiados se aislaron E.coli productores de F17 y/o stx+, eaeA+, ehly+. Dos cepas stx1+, eaeA+, ehly+ de los serotipos O153:H- y O159:H- expresaron fimbrias F17. Por su reactividad con sueros específicos y un peso molecular aparente de 20 kDa se las identificó como pertenecientes a los subtipos c y a, respectivamente. En el caso de la cepa del serotipo O159:H- se detectó también la presencia de una fimbria de 18.5 kDa con reactividad cruzada con antisuero F17 que podría corresponder a F17d o a una nueva variante dentro de la familia F17. El resto de los E.coli expresaron fimbrias F17 (c y a/18.5) ó bien fueron productores de Stx, correspondiendo a los patotipos O5:H-, stx1, eaeA+, ehly+; O26:H-(H11), stx2, eaeA+, ehly+; O26:H-(H11), stx1, eaeA+, ehly+; y O111:H-, stx1, eaeA+, ehly+. Todos los E.coli F17+ mostraron adherencia a villi de ternero. Ninguna de las cepas expresó los antígenos F5, F41, CS31A ni produjo toxina StaP. La adquisición de mecanismos de colonización no reportados previamente en cepas de E.coli productoras de toxinas Shiga plantea la posibilidad de la aparición de clones con un mayor perfil de virulencia en bovinos y/o humanos. [less ▲]

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See detailAsociación entre genotipo y evolución patogénica en variantes naturales del virus de la leucosis bovina
Trono, K.; Dus Santos, M.J.; Wigdorovitz, A. et al

Poster (2002, September 23)

Asociacion entre genotipo y evolucion patogénica en variantes naturales del virus de la leucosis bovina. Karina Trono1, María José DusSantos1, Andrés Wigdorovitz1, Sabrina Rodríguez1, Sebastián Chiavenna1 ... [more ▼]

Asociacion entre genotipo y evolucion patogénica en variantes naturales del virus de la leucosis bovina. Karina Trono1, María José DusSantos1, Andrés Wigdorovitz1, Sabrina Rodríguez1, Sebastián Chiavenna1, Fernando Ardila2, Manuel Borca1 y Consuelo Carrillo1 1 Instituto de Virología. CICVyA. INTA Castelar. 2 Instituto de Genética. CNIA. INTA Castelar. La relación entre el genotipo y las formas de manifestación patogénica está demostrada para ciertos Retrovirus, y se ha descripto la presencia de determinantes de patogenia, tropismo tisular y virulencia en regiones del genoma proviral como LTR y env Sin embargo, esto no ha sido descripto para el Virus de la Leucosis Bovina (VLB), donde aún no se conocen las causas de desarrollo de las diferentes formas de la infección: el linfosarcoma (LS), la linfocitosis persistente (PL), o la forma aleucémica (AL). Con el objetivo de conocer si existe relación entre el genotipo proviral del VLB y las formas de evolución patogénica de animales infectados se secuenció la zona R-U5 de las regiones genéticas provirales LTR aisladas de 36 animales infectados naturalmente y con diferentes formas de evolución: 14 con linfosarcoma (LS), 11 con Linfocitosis Persistente (PL) y 10 portadores asintomáticos o aleucémicos (AL). La secuencia de nucleótidos reveló la presencia de 4 cambios fijos y característicos de los LTR de provirus aislados de linfosarcoma (100%), 1 en la región R y 3 en U5. Estos cambios se encontraron ausentes en la mayoría (16/22) de los provirus aislados de formas asintomáticas (AL/PL). La alta asociación entre los cambios de nucleótidos y las formas de patogenia, fue demostrada estadísticamente y permitió agrupar en forma diferencial a los provirus aislados de linfosarcoma, con respecto a los provirus de AL/PL y confirmar la existencia de una secuencia consenso de LS. Este trabajo define por primera vez la presencia de marcadores de patogenia asociados al genotipo viral de VLB así como la existencia de una secuencia de nucleótidos propia de LS en la región LTR del genoma proviral de VLB. [less ▲]

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See detailThe Asp298Asn missense mutation in the porcine melanocortin-4 receptor (MC4R) gene can be used to affect growth and carcass traits without an effect on meat quality
Van den Maagdenberg, Karijn; Stinckens, Anneleen; Claeys, Eric et al

in Animal (2007), 1(8), 1089-1098

A promising tool to improve daily gain in pigs is the missense mutation (Asp298Asn) in the melanocortin-4 receptor (MC4R) gene, especially in the Belgian pig industry where the slow-growing Pietrain breed ... [more ▼]

A promising tool to improve daily gain in pigs is the missense mutation (Asp298Asn) in the melanocortin-4 receptor (MC4R) gene, especially in the Belgian pig industry where the slow-growing Pietrain breed is very frequently used as the sire breed The MC4R is expressed in the appetite-regulating region of the brain where it regulates feed intake and energy balance. The mutation has been associated with differences in fatness, daily gain and feed intake. However, less information on the correlated effects on meat quality is available. In order to evaluate the influence of the MC4R mutation on carcass and meat quality parameters, a total of 1155 pigs of a four-way cross were slaughtered at an average live weight of 109 kg, and data about daily live-weight gain, carcass and meat quality were collected Allelic frequencies were 0.69 for the G-allele (298Asp variant or well-conserved variant) and 0.31 for the A-allele (298Asn variant or the mutated variant). Barrows and gilts were almost equally distributed in this population with, respectively, 49.9% and 50.1%. Moreover, independent of this mutation, the relationship between average daily gain (ADG) and carcass on the one hand and meat quality traits on the other hand was evaluated in this population. A significant positive influence of the MC4R mutation on ADG (P < 0.001) was found accompanied by a higher fat thickness (P < 0.05) and a lower carcass lean meat content (P < 0.01), whereas muscle thickness and carcass conformation traits were not affected. The effects on meat quality traits were not significant, except for a lower shear force (P = 0.054) and a higher intramuscular fat content (P = 0.052) in AA animals. In the longissimus, pH and pork quality meter (PQM) values were not influenced, and effects on drip loss and colour were not apparent. Residual correlation coefficients between ADG and carcass lean meat content on the one hand and meat quality traits on the other hand were generally very low (vertical bar r vertical bar > 0.1). Higher ADG, higher carcass fat thickness and lower carcass lean meat content were correlated with slightly lower shear force values (vertical bar r vertical bar similar to 0.1, P < 0.05). In conclusion, in the studied population, the Asp298Asn mutation in the MC4R gene was associated with improved daily gain, higher carcass fatness and almost no effect on meat quality traits. [less ▲]

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See detailAsparagine
Fredericq, Léon ULg

in Richet, Charles (Ed.) Dictionnaire de physiologie. 001. Tome I (1895)

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See detailAspartic proteinase members secreted by the ruminant placenta: Specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins
Perenyi, Z. S.; Szenci, O.; Drion, Pierre ULg et al

in Reproduction in Domestic Animals (2002), 37(6), 324-329

Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in the maternal blood can be a good indicator of the presence of a live embryo. As certain aspartic proteinases are present in biological fluids in physiological and pathological conditions at various concentrations, it was necessary to determine the specificity of three radioimmunoassay (RIA) systems currently used for the detection of PAG molecules. Commercially available members of the aspartic proteinase family like pepsinogen, pepsin, chymosin, rennet, cathepsin D and renin were tested in a wide concentration range (10 ng/ml - 1 mg/ml). Pepsinogen cross-reacted in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 50 microg/ml and 500 microg/ml concentrations, respectively. In the presence of pepsin, cross-reaction was observed in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 500 microg/ml and 1 mg/ml concentrations, respectively. Chymosin and rennet could cross-react in RIA 2 and RIA 3, while renin and cathepsin D did not decrease the binding of the tracer to antisera more, than that of the minimal detection limit. As the plasma/serum concentrations of the examined aspartic proteinases reported in the literature were outside the concentration range where cross-reaction was observed, it can be concluded that these RIA systems were specific for the detection of PAGs in biological fluids. [less ▲]

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See detailAspect diagnostic du nodule thyroïdien isolé
Beckers, Albert ULg

Scientific conference (1996, January 20)

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See detailAspect génétique de la fertilité. Possibilité d'amélioration par sélection
Kafidi, N.; Chapaux, Ph.; Leroy, Pascal ULg et al

(1988)

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See detailAspect in Egyptian: a Semantic Approach
Winand, Jean ULg

Conference (2005, September 10)

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