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See detailIdentification of virulotypes and serotypes of enteropathogenic (EPEC) and Shigatoxigenic (STEC) Escherichia coli from healthy cattle at slaughterhouses in Wallonia.
Takaki, Shino; Duprez, Jean-Noël ULiege; Fakih, Ibrahim et al

Poster (2016, September)

Escherichia coli producing the attachment-effacement (AE) lesion (EPEC) and/or Shiga toxins (STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the ... [more ▼]

Escherichia coli producing the attachment-effacement (AE) lesion (EPEC) and/or Shiga toxins (STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the intestines of healthy cattle. Besides the O157:H7 serotype, which is the main serotype causing STEC outbreaks in the world EPEC and STEC can belong to dozens of O serogroups. Of them, 9 have been frequently identified worldwide: O5, O26, O103, O104, O111, O118, O121, O145 and O165. The aim of this study is to identify the virulotypes and the O serotypes of EPEC and STEC isolated from healthy cattle at slaughterhouses in Wallonia. A total of 245 faeces (216 <1year-old bulls, 25 cows and 4 heifers) were sampled between April and June 2014 in 2 slaughterhouses in Wallonia and grown overnight at 37°C in Lauryl sulfate Enterobacteriaceae selective broth. The enrichment broths were assayed with an stx1, stx2 (Shiga toxin) and eae (AE lesion) triplex PCR and positive broths were inoculated onto 4 agar media: McConkey’s, Chromagar ES, Chromagar ES with tellurite and Chromagar STEC. Up to ten colonies per plate were picked up, sub-cultured and tested by the colony hybridization assay with gene probes targeting the stx1, stx2 and eae genes. The triplex PCR was again performed on all probe-positive isolates. The PCR-positive E. coli were subsequently assayed with two pentaplex PCR targeting the specific genes coding for the ten O serogroups listed above. Of the 2563 sub-cultured isolates, 744 isolates (29%) from 62 animals (25%) tested positive with the colony hybridization assay. Of them, 687 isolates (92%) from 59 animals were positive with the triplex PCR and the results of both tests were in agreement for 617 isolates (83%). One to 29 isolates per animal were probe- and PCR-positive. The positive isolates grew on Chromagar STEC (379; 55%), on Chromagar ES with tellurite (189; 28%), on Chromagar ES (62; 9%) or on McConkey’s agar (57; 8%). The most frequent virulotypes were eae+ (EPEC: 372 isolates; 54%), eae+stx1+ (AE_STEC: 119 isolates; 17%) and stx2+ (STEC: 118 isolates; 17%). In some animals different virulotypes were identified. The serogrouping with the two pentaplex PCR is in progress. AE-STEC, EPEC and STEC are excreted by 25% of the healthy cattle at slaughterhouses in Wallonia and different virulotypes can be excreted by the same animal. Conversely the methodology followed gives no precise idea of the actual level of excretion since the hybridization and PCR were performed after enrichment in selective broth. Therefore multiple isolates belonging to the same virulotype might represent the same clone. Identification of the serogroups and comparison by Pulsed Field Gel Electrophoresis should help to clarify that point. Quantitative (q)PCR is today the best method to quantify bacterial excretion, but is more expensive. The results of the hybridization and PCR correspond to between 80 and 90%. Though the colony hybridization is still useful for large-scale surveillance it needs radioactive probes for highest sensitivity and is more time-consuming than PCR. Therefore the PCR should be the first routine choice if it can be automatized at large scale. Further steps are the confirmation of the PCR results of the 70 isolates with different hybridization and PCR results and the identification of the serogroups with the two pentaplex PCR and later with PCR for the other serogroups, to compare them with isolates from young diarrhoeic calves and from humans. [less ▲]

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See detailIdentification of VZV ORF9p potential cellular partners that could be important for the viral egress.
Lebrun, Marielle ULiege; riva, laura; Rambout, Xavier ULiege et al

Poster (2015, July 26)

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate ... [more ▼]

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate of the viral kinase ORF47p and that its ORF47p-dependent phosphorylation is important for the secondary envelopment process. We also have identified an acidic cluster (AC) within the protein that is important for its correct localization in the infected cells and for the interaction with ORF47p. The recombinant VZV expressing ORF9p-ΔAC presents an accumulation of capsids in the perinuclear space. ORF9p seems then to play an important role in several steps of the egress process. In this context, we sought to identify cellular partners of ORF9p that might be important for these functions. We performed a yeast two hybrid screen against the human ORFeome 5.1. and picked out 44 candidates among which 5 proteins playing roles in membrane organization and targeting. We currently are trying to confirm these interactions in infected cells and to assess the role of these interactions for the viral lytic cycle. [less ▲]

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See detailIdentification of walnut husk (Juglans regia L.) volatiles and the behavioural response of the invasiveWalnut Husk Fly, Rhagoletis completa Cresson
Sarles, Landry ULiege; Boullis, Antoine ULiege; Fassotte, Bérénice ULiege et al

in Pest Management Science (2017)

Abstract BACKGROUND: Several European countries are important walnut (Juglans regia L.) producers. However, these countries must contendwith the recent introduction of theWalnut Husk Fly,Rhagoletis ... [more ▼]

Abstract BACKGROUND: Several European countries are important walnut (Juglans regia L.) producers. However, these countries must contendwith the recent introduction of theWalnut Husk Fly,Rhagoletis completa Cresson (Diptera, Tephritidae),which is causing severe economic losses, especially in organic production. Because most Tephritid fruit flies use kairomones in their search for host plants, we hypothesise that this highly specialist species orients toward the volatile blend released by walnut husks. RESULTS:We collected, identified, and quantified the volatile organic chemicals (VOCs) released by walnut husks from themost commonly cultivated variety in France (Franquette). Then, the behavioural response of R. completa toward synthetic odour blends was recorded in dual choice assays conducted in net cages. A total of 26 VOCs were identified, with 𝜶-pinene, 𝜷-pinene, trans-linalool, eugenol, and tetradecane representing the major constituents. In the dual choice assay, male and female R. completa were strongly attracted to synthetic blend that includedmost of the identified husk VOCs. CONCLUSION:When searching for a host plant, R. completa use host fruit kairomones. The potential of these semiochemicals in monitoring andmanagement of this quarantine pest is discussed. [less ▲]

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See detailIdentification of wire rope isolators using the restoring force surface method
Kerschen, Gaëtan ULiege; Lenaerts, Vincent; Golinval, Jean-Claude ULiege

in International Conference on Structural Systems Identification, Kassel, 2001 (2001)

The restoring force surface method offers an efficient and reliable identification of non-linear single-degree-of-freedom systems. The method may be extended to multidegree-of-freedom systems but by ... [more ▼]

The restoring force surface method offers an efficient and reliable identification of non-linear single-degree-of-freedom systems. The method may be extended to multidegree-of-freedom systems but by loosing the key advantage of the method which lies in the two-dimensional representation for single-degree-of-freedom systems. An experimental application of the restoring force surface method is considered in the present paper. The structure investigated consists of wire rope isolators mounted between a load mass and a base mass. These helical isolators were found to be characterised by a non-linear behaviour. The results obtained are discussed in details and the advantages and drawbacks of the method are underlined. [less ▲]

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Peer Reviewed
See detailIdentification Of Yhr019 In Saccharomyces Cerevisiae Chromosome VIII As The Gene For The Cytosolic Asparaginyl tRNA Synthetase
Landrieu, I.; Vandenbol, Micheline ULiege; Leberman, R. et al

in Yeast (1998), 14(6),

Detailed reference viewed: 9 (0 ULiège)
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See detailIdentification of yield locus parameters of metals using inverse modeling and full field DIC
Lecompte, D.; Cooreman, S.; Sol, H. et al

in Proceedings of the 7th national congress on theoretical and applied mechanics (2006)

The basic principle of the inverse modeling procedure as it is used for parameter identification is the generation of a complex and heterogeneous deformation field that contains as much information as ... [more ▼]

The basic principle of the inverse modeling procedure as it is used for parameter identification is the generation of a complex and heterogeneous deformation field that contains as much information as possible about the parameters to be identified. One way of obtaining such a non-homogeneous deformation is by making the geometry of the specimen less regular. Another possibility is to make the loading conditions more complex. In this paper both options are actually combined by using the concept of a biaxial tensile test on a perforated cruciform specimen. In the present paper, the work hardening of the material is assumed to be isotropic and it is described by a Swift law. The yield locus is modeled by the anisotropic Hill48 criterion. The optimization technique used is a constrained gradient based Newton-type routine, which means that in every iteration step, a sensitivity calculation has to be performed in order to indicate the direction in which the parameters are to be optimized. The functional to be minimized is a least-squares expression of the discrepancy between the measured and the simulated strain fields at a certain load. The numerical routines as well as the identification results of the different parameters, based on simulated strain fields, are discussed. [less ▲]

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See detailIdentification of ‘Textsorten’ in the Late Egyptian Corpus
Gohy, Stéphanie ULiege; Martin Leon, Benjamin ULiege

in Winand, Jean; Polis, Stéphane (Eds.) Texts, Languages & Information. Technology in Egyptology. Selected papers from the meeting of the Computer Working Group of the International Association of Egyptologists (Informatique & Égyptologie), Liège, 6-8 July 2010 (2010, July 08)

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See detailIdentification On Commercialized Products Of Aflp Markers Able To Discriminate Slow- From Fast-Growing Chicken Strains
Fumiere, Olivier; Dubois, Marc; Gregoire, Dimitrie et al

in Journal of Agricultural and Food Chemistry (2003), 51(5), 1115-1119

The European chicken meat market is characterized by numerous quality marks: “Label de Qualité Wallon” in Belgium, “Label Rouge” in France, denominations of geographical origin, organic agriculture, etc ... [more ▼]

The European chicken meat market is characterized by numerous quality marks: “Label de Qualité Wallon” in Belgium, “Label Rouge” in France, denominations of geographical origin, organic agriculture, etc. Most of those certified productions have specifications requiring the use of slow-growing chicken strains. The amplified fragment length polymorphism (AFLP) technique has been used to search molecular markers able to discriminate slow-growing chicken strains from fast-growing ones and to authenticate certified products. Two pairs of restriction enzymes (EcoRI/MseI and EcoRI/TaqI) and 121 selective primer combinations were tested on individual DNA samples from chicken products essentially in carcass form that were ascribed as belonging to either slow- or fast-growing strains. Within the resulting fingerprints, two fragments were identified as type−strains specific markers. One primer combination gives a band (333 bp) that is specific for slow-growing chickens, and another primer pair generates a band (372 bp) that was found to be characteristic of fast-growing chickens. The two markers were isolated, cloned, and sequenced. The effectiveness and the specificity of the two interesting determinants were assessed on individuals of two well-known strains (ISA 657 and Cobb 500) and on commercialized products coming from various origins. [less ▲]

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See detail"Identification organisationnelle et changement: les logiques identitaires à l'épreuve du New Public Management"
Rondeaux, Giseline ULiege

Doctoral thesis (2012)

Depuis près de 30 ans, nombre de pays se sont engagés dans la réforme de leur administration publique, inspirée du New Public Management (NPM), inspirant de multiples travaux et débats sur ses fondements ... [more ▼]

Depuis près de 30 ans, nombre de pays se sont engagés dans la réforme de leur administration publique, inspirée du New Public Management (NPM), inspirant de multiples travaux et débats sur ses fondements et ses déclinaisons, ses avantages et inconvénients, sa pertinence, ses paradoxes ou encore les difficultés liées à sa mise en oeuvre ou à son évaluation. Construit comme une critique des règles de l’administration bureaucratique, ce modèle managérial constitue une profonde remise en cause de celle-ci en termes d’organisation, de management, de GRH et de valeurs fondamentales. Les réformes inspirées du NPM sont aussi à la base d’une redéfinition de l’identité organisationnelle institutionnalisée de l’administration (Du Gay, 1996) : l’organisation s’interroge sur ce qu’elle est, ce qu’elle devient, ce qu’elle veut être, propulsant l’identité organisationnelle au premier plan des préoccupations. Alors que le processus de modernisation de l’administration vise à redéfinir la réponse à la question « qui sommes-nous en tant qu’organisation ? », de quelle manière les membres de celle-ci en font-ils l’expérience? Au travers de l’analyse de trois cas de réforme d’administrations publiques, notre thèse s’intéresse aux dynamiques d’identification à l’oeuvre en regard de la perception des évolutions du contexte par les individus. Nous mettons en évidence le lien qui existe entre les processus d’identification organisationnelle et deux dimensions spécifiques à chaque contexte en évolution : le caractère plus ou moins radical de changement d’identité organisationnelle institutionnalisée que suppose la réforme mise en œuvre, et le degré de maturité de ce processus de réforme, ou en d’autres termes la mesure dans laquelle la réforme est plus ou moins perceptible dans la transformation du contexte même. Au départ des différents éléments relevés dans chacun de nos terrains, notre thèse illustre concrètement comment la mise en œuvre d’un management polyphonique du processus de changement peut contribuer de manière positive à l’identification organisationnelle des membres de ces organisations en évolution. Cette approche mène à réfléchir autrement à la gestion du changement au travers de la prise en compte de cette diversité de positionnements identitaires. Elle conduit en outre à une réflexion plus large sur la mise en œuvre du NPM et sur l’administration publique de demain. [less ▲]

Detailed reference viewed: 251 (53 ULiège)
See detailIdentification par empreintes génétiques des espèces animales entrant dans une chaîne alimentaire
Haezebroeck, Valérie; Renaville, Robert ULiege; Bertozzi, Carlo et al

in Cinquième Carrefour des Productions animales. Quels systèmes de Productions Animales pour le 21ème siècle? (2000, January 26)

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See detailAN IDENTIFICATION STUDY ON BOTTLENOSE DOLPHINS (TURSIOPS TRUNCATUS) IN NORTHEAST PATAGONIA, ARGENTINA
Vermeulen, Els ULiege; Cammareri, Alejandro; Failla, Mauricio et al

Poster (2008, October 13)

In Argentina, bottlenose dolphin (Tursiops truncatus) sightings decreased notably since the late 80s in regions where it used to be frequent to observe them. Nowadays, Northeast Patagonia is one of the ... [more ▼]

In Argentina, bottlenose dolphin (Tursiops truncatus) sightings decreased notably since the late 80s in regions where it used to be frequent to observe them. Nowadays, Northeast Patagonia is one of the few regions where they still can be seen frequently, although local increasing human activities result in increasing need for information. In general, photo-identification has been established as a helpful tool in cetacean research. However, only few studies have applied this method to bottlenose dolphins in Argentina. This study is therefore aimed to obtain basic information concerning bottlenose dolphins through their identification, this way contributing to their conservation. Land-based observations were made in the northern Gulf of San Matías, Patagonia, Argentina, from August 2006 up to June 2008. Over 15.000 digital pictures of dorsal fins were analysed using the computer assisted identification systems FinEx and FinMatch. Dolphins re-identified during all four seasons in one year were defined as residents. The degree of residency was further estimated by the re-identification frequency (RF); non-resident (RF=1-3) - occasional (RF=4-7) - frequent (RF=8-11) - common (RF¿12). In total, 221 surveys were conducted with an average observation effort of 3.2h per survey, resulting in a total observation effort of 915h of which 124h were spent with 182 dolphin groups. A total of 50 dolphins were identified of which the vast majority (82%) was photographed only in the Natural Protected Area Bahía San Antonio (NPABSA), due to the higher observation effort in this region. Nevertheless, 12% of the catalogued dolphins could be photographed in both NPABSA and the Río Negro estuary, indicating that their home-range might include at least the whole northern region of the Gulf San Matias. Dolphins could be re-identified up to 13 days with 54% showing a degree of residency for NPABSA. This study shows the first data concerning bottlenose dolphins in Northeast Patagonia, but further investigation is highly necessary to improve their conservation. Therefore, the obtained photo-identification catalogue from the presented study is meant to serve as a primary tool for progressing research concerning this species in Argentina. [less ▲]

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See detailIdentification, cartographie et gestion des Forêts à Haute Valeur de Conservation : cas de la concession Pallisco, Cameroun
Julve Larrubia, C.; Sépulchre, F.; Philippart, Julien ULiege et al

Conference (2009)

Detailed reference viewed: 52 (7 ULiège)
See detailIdentification, characterization and expression profiling of the tomato gene TomLoxF
Mariutto, Martin ULiege; Duby, Franceline ULiege; Adam, Akram et al

Poster (2010, January 26)

Detailed reference viewed: 41 (11 ULiège)
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See detailIdentification, Characterization and Full-Length Sequence Analysis of a Novel Polerovirus Associated with Wheat Leaf Yellowing Disease
Zhang, Peipei; Liu, Yan; Liu, Wenwen et al

in Frontiers in Microbiology (2017), 8(1689),

Detailed reference viewed: 43 (2 ULiège)
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See detailIdentification, Design and Evaluation of Pharmacological tools for the orphan GPCR GPR22
Geubelle, Pierre ULiege; Gilissen, Julie ULiege; Dupuis, Nadine ULiege et al

Poster (2015, January 27)

GPCRs are the largest family of membrane receptors and are characterized by 7 transmembrane domains. GPR22 is a GPCR that has no known endogenous ligand and is thus considered "orphan". Its presence ... [more ▼]

GPCRs are the largest family of membrane receptors and are characterized by 7 transmembrane domains. GPR22 is a GPCR that has no known endogenous ligand and is thus considered "orphan". Its presence situated at the heart and brain levels makes it a potential target for new therapeutic pathways. This study consist in the identification of a synthetic ligand of GPR22 receptor to use it as a pharmacological tool in the study of the signaling channels of GPR22 in order to understand its role and to validate it as a new therapeutic target. The initial hypothesis was that GPR22 is coupled to the Gαi protein. [less ▲]

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See detailIdentification, Design and Evaluation of Pharmacological tools for the orphan GPCR GPR22
Geubelle, Pierre ULiege; Gilissen, Julie ULiege; Dupuis, Nadine ULiege et al

Poster (2014, November 21)

GPCRs are the largest family of membrane receptors and are characterized by seven transmembrane domains. This family of receptors is currently the most successfully targeted protein for therapeutic ... [more ▼]

GPCRs are the largest family of membrane receptors and are characterized by seven transmembrane domains. This family of receptors is currently the most successfully targeted protein for therapeutic purposes. GPR22 is a GPCR that was discovered in 1997. It has no known endogenous ligand and is thus considered "orphan". Its presence situated at the heart and brain levels makes it a potential target for new therapeutic pathways. The only information about its signaling channel could be its coupling with G proteins. This study consist in the identification of a synthetic ligand of GPR22 receptor to use it as a pharmacological tool in the study of the signaling channels of GPR22 in order to understand its role and to validate it as a new therapeutic target. The initial hypothesis was that GPR22 is coupled to the Gαi protein. [less ▲]

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See detailIdentification, Health Monitoring and Damage Detection of Structural Dynamic Systems: Results Obtained Within the European COST Research Network
Golinval, Jean-Claude ULiege; Link, Michael

Book published by Academic Press (2003)

This special issue presents the results of a research action organised within the framework of the European CO-operation in the field of Scientific and Technical research (COST). The COST F3 Action ... [more ▼]

This special issue presents the results of a research action organised within the framework of the European CO-operation in the field of Scientific and Technical research (COST). The COST F3 Action started on June 25, 1997 for a duration of four years with the aim of co-ordinating national funded research efforts in the field of structural dynamics. Thirteen European countries were participating in this Action: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Italy, The Netherlands, Portugal, Spain, Switzerland and the United Kingdom plus the Joint Research Centre of the European Union in Ispra, Italy and the European Commission. The research network that has been developed within COST Action F3 has allowed not only to exchange research ideas and results, but also to support young researchers in the field. Throughout its four years of duration, COST Action F3 brought together the foremost researchers in Europe in a series of activities associated with three threads of structural dynamics : • model updating methods ; • structural health monitoring and damage detection ; • identification of non-linear systems. These activities included the organisation of major international conferences, the exchange of research staff through Short Term Scientific Missions and the organisation of benchmarking exercises. It is the latter activity which concerns this special issue. Thirty-one papers from altogether seven European countries are presented. [less ▲]

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