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See detailMeasurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep
El Amiri, B.; Karen, A.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2007), 42(3), 257-262

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer ... [more ▼]

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG(57+59kDa)) and R805 (against ovPAG5(58+61kDa)) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p < 0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding. [less ▲]

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See detailMeasurement of oxygen consumption and cardio-respiratory parameters in ridden horse
Votion, Dominique ULg; Caudron, Isabelle; Lejeune, jean-Philippe et al

in Pferdeheilkunde (2006, August), 22(5), 619-624

This study aimed at testing the feasibility of using the Cosmed K4 b2® portable telemetric gas analysis system to record metabolic parameters in ridden exercising horses. Adaptation of the Cosmed K4 b2® ... [more ▼]

This study aimed at testing the feasibility of using the Cosmed K4 b2® portable telemetric gas analysis system to record metabolic parameters in ridden exercising horses. Adaptation of the Cosmed K4 b2® to horses’ specificities included the design of an airtight face-mask (Equimask®) adapted to a hackamore bridle to allow ridding the horse. The portable system enables the recording of tidal volume (VT), respiratory rate (RR), minute expired volume (VE), pulmonary oxygen uptake (VO2), carbon dioxide delivery (VCO2), heart rate (HR) and speed. Four saddle horses equipped with the system completed 3 treadmill tests (TT1 to TT3) and 1 field test (FT) consisting of galloping phases at incremental speeds. Horses were sampled for blood lactate (LA) during the tests. The successive treadmill tests showed the influence of stress on measurements: with horses becoming accustomed to treadmill, LA and HR were reduced between TT1 and TT3. As VO2 is related to cardiovascular function, influence of stress resulted in higher VO2 in TT1 vs. TT3. The VO2 reached at maximal speed during treadmill tests was lower than the expected maximal aerobic power (VO2max) for trained saddle horses. During FT, the VO2 reached at the final gallop was lower than the VO2 recorded during any of the treadmill tests thus reflecting the increasing difficulty to reach VO2max with ridden horses in field conditions. This study demonstrated that cardiorespiratory parameters may be obtained in ridden horses during a field trial and opens new perspectives in the follow-up of sport horses in real field conditions. [less ▲]

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See detailMeasurement of PCB-153 and DDE in 20 μL dried-blood spots
L'Homme, Benjamin ULg; Focant, Jean-François ULg

in Organohalogen Compounds (2012), 74

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See detailMeasurement of PCDDs, PCDFs, and non-ortho-PCBs by comprehensive two-dimensional gas chromatography-isotope dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS)
Focant, Jean-François ULg; Reiner, Eric J.; MacPherson, Karen et al

in Talanta (2004), 63(5), 1231-1240

Comprehensive two-dimensional gas chromatography with isotope-dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) was used to measure polychlorinated dibenzo-p-dioxin (PCDD), polychlorinated ... [more ▼]

Comprehensive two-dimensional gas chromatography with isotope-dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) was used to measure polychlorinated dibenzo-p-dioxin (PCDD), polychlorinated dibenzofuran (PCDF), and coplanar polychlorinated biphenyl (cPCB) concentrations in ash, sediment, vegetation, and fish samples. The GC x GC capability was achieved by using a quad jet, dual stage, thermal modulator. Zone compression of the GC peaks from modulation resulted in a significant increase of the signal intensity over classical GC-IDTOFMS. The GC x GC column set used an Rtx-Dioxin 2 phase as the first dimension (D-1) and an Rtx-500 as the second dimension (D-2). The chromatographic separation of the 17 PCDD/Fs and the 4 cPCBs was attained in D-1 except for 2,3,7,8-TCDD and CB126 for which deconvoluted ion currents (DIC) were required to be reported separately. The Rtx-500 phase separated the bulk matrix interfering compounds from the target analytes in D-2. The instrumental limit of detection (iLODs) was 0.5 pg for 2,3,7,8-TCDD. The calibration curves showed good correlation coefficients for all the compounds investigated in the concentration range of 0.5-200 pg. GC x GC-IDTOFMS results compared favorably to those from conventional isotope-dilution one-dimensional gas chromatography-high resolution mass spectrometry (GC-IDHRMS). The comprehensive mass analysis of the TOFMS further permitted the identification of other contaminants of concern in the samples. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailMeasurement of progesterone concentration in plasma samples: comparison of three radioimmunoassay procedures
Legras, B.; Beckers, Jean-François ULg; Sulon, J.

in Reproduction (Cambridge, England). Abstract Series (2004), 31

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See detailMeasurement of proteoglycans, elastase, collagenase and protein in synovial fluid in inflammatory and degenerative arthropathies
Gysen, Ph; Malaise, Michel ULg; Gaspar, S. et al

in Clinical Rheumatology (1985), 4(1), 39-50

The number of leucocytes and the concentrations of protein, proteoglycans (PG), elastase a1 proteinase inhibitor complexes and collagenolytic activity were measured in the synovial fluid (SF) of 15 ... [more ▼]

The number of leucocytes and the concentrations of protein, proteoglycans (PG), elastase a1 proteinase inhibitor complexes and collagenolytic activity were measured in the synovial fluid (SF) of 15 patients with rheumatoid arthritis (RA) and 18 with osteoarthritis (OA). The mean levels of protein and collagenase and the number of leucocytes were higher in RA than in OA SF. However, the mean level of PG was higher of OA SF than in RA. In the latter, they were principally in the form of monomers and fragments while in the former they were in the form of aggregates and monomers. There was a direct relationship between the concentration of E-a/Pi and either the number of white cells or the concentration of synovial proteins, suggesting that the measurement of E-a/Pi complexes is a biochemical index of the local inflammatory reaction. There was an inverse correlation between the concentrations ofPG and E-a/Pi which may reflect the effect of degradation in PG of elastase and other enzymes released at the same time. Finally, there was a direct relationship between the concentration of E-a/Pi and collagenase which may be the reflection of a simultaneous release of various enzymes from leucocytes and macrophages. [less ▲]

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See detailMeasurement of pulmonary inertance in Equids
Art, Tatiana ULg; Lekeux, Pierre ULg; Gustin, Pascal ULg et al

in European Respiratory Journal (1989), 2

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See detailMeasurement of quality of life in cardiac rehabilitation
Dupuis, Gilles; Taillefer, Marie Christine; Etienne, Anne-Marie ULg et al

in Jobin, Jean; Maltais, François; LeBlanc, Pierre (Eds.) et al Advances in Cardiopulmonary rehabilitation (2000)

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See detailMeasurement of radiative lifetimes and determination of transition probabilities of astrophysical interest in HoIII
Biémont, Emile ULg; Palmeri, P.; Quinet, Pascal ULg et al

in Monthly Notices of the Royal Astronomical Society (2001), 328(4), 1085-1090

Lifetimes of six levels belonging to the 4f(10)6p configuration of Ho III have been measured for the first time using the time-resolved laser-induced fluorescence method. They are compared with ... [more ▼]

Lifetimes of six levels belonging to the 4f(10)6p configuration of Ho III have been measured for the first time using the time-resolved laser-induced fluorescence method. They are compared with multiconfigurational pseudo-relativistic Hartree-Fock calculations. A very good agreement is found. Using the experimental lifetimes and theoretical branching fractions, a first set of transition probabilities of astrophysical interest has been obtained for this ion. [less ▲]

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See detailMeasurement of respiratory function by impulse oscillometry in horses
Van Erck, Emmanuelle; Votion, Dominique ULg; Art, Tatiana ULg et al

in Equine Veterinary Journal (2004), 36(1), 21-28

Reasons for performing study: Due to technical implementations and lack of sensitivity, pulmonary function tests are seldom used in clinical practice. Impulse oscillometry (IOS) could represent an ... [more ▼]

Reasons for performing study: Due to technical implementations and lack of sensitivity, pulmonary function tests are seldom used in clinical practice. Impulse oscillometry (IOS) could represent an alternative method. Objectives: To define feasibility, methodology and repeatability of IOS, a forced oscillation technique that measures respiratory resistance (R-rs) and reactance (X-rs) from 5 to 35 Hz during spontaneous breathing, in horses. Methods: Using 38 healthy horses, R-rs and X-rs reference values were defined and influence of individual biometrical parameters was investigated. In addition, IOS measurements of 6 horses showing clinical signs of heaves were compared to those of 6 healthy horses. Results: Airtightness and minimal dead space in the facemask were prerequisites to IOS testing and standardisation of head position was necessary to avoid variations in R-rs due to modified upper airway geometry. In both healthy and diseased animals, measurements were repeatable. In standard-type breeds, the influence of the horse's size on IOS parameters was negligible. An increase in R-5Hz greater than 0.10 kPa/l/sec and R-5Hz>R-10Hz, combined with negative values of X-rs between 5 and 20 Hz, was indicative of heaves crisis. Conclusions: IOS is a quick, minimally invasive and informative method for pulmonary function testing in healthy and diseased horses. Potential relevance: IOS is a promising method for routine and/or field respiratory clinical testing in the equine species. [less ▲]

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See detailMeasurement of respiratory impedance in healthy calves using the impulse oscillation technique - physiological and methodological aspects
Reinhold, Petra; Smith, H.-J.; Langenberg, Annelie et al

in Veterinary Journal (1998), 155(1), 27-38

The aim of the present study was to investigate methodological and physiological aspects of complex respiratory impedance measurements in calves using the impulse oscillation technique. To apply the ... [more ▼]

The aim of the present study was to investigate methodological and physiological aspects of complex respiratory impedance measurements in calves using the impulse oscillation technique. To apply the system to animals, a tightly fitting face mask was used. The measuring system was modified by placing a flexible tube between the pneumotachograph and the loudspeaker. Complex respiratory impedance measurements were not significantly influenced by the modification. Linear correlations were found between body weight and respiratory impedance; as body weight increased, resistance decreased and reactance increased. The methodological variability of impedance measurements using the impulse technique was found to be extremely small. Diurnal variations of respiratory impedance were considered to be the main source of intra-individual variability. When comparing different animals (even when healthy and matched for age and body weight) our results suggest that inter-individual biological variability must be carefully considered [less ▲]

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See detailMeasurement of ribosomal RNA turnover in vivo by use of deuterium-labeled glucose.
Defoiche, Julien; Zhang, Yan; Lagneaux, Laurence et al

in Clinical Chemistry (2009), 55(10), 1824-33

BACKGROUND: Most methods for estimation of rates of RNA production are not applicable in human in vivo clinical studies. We describe here an approach for measuring ribosomal RNA turnover in vivo using [6 ... [more ▼]

BACKGROUND: Most methods for estimation of rates of RNA production are not applicable in human in vivo clinical studies. We describe here an approach for measuring ribosomal RNA turnover in vivo using [6,6-(2)H(2)]-glucose as a precursor for de novo RNA synthesis. Because this method involves neither radioactivity nor toxic metabolites, it is suitable for human studies. METHODS: For method development in vitro, a lymphocyte cell line (PM1) was cultured in the presence of [6,6-(2)H(2)]-glucose. RNA was extracted, hydrolyzed enzymatically to ribonucleosides, and derivatized to either the aldonitrile tetra-acetate or the pentafluoro triacetate derivative of the pentose before GC-MS. We identified optimum derivatization and analysis conditions and demonstrated quantitative incorporation of deuterium from glucose into RNA of dividing cells. RESULTS: Pilot clinical studies demonstrated the applicability of this approach to blood leukocytes and solid tissues. A patient with chronic lymphocytic leukemia received [6,6-(2)H(2)]-glucose (1 g/kg) orally in aliquots administered every 30 min for a period of 10 h. When we analyzed CD3(-) B cells that had been purified by gradient centrifugation and magnetic-bead adhesion, we observed deuterium enrichment, a finding consistent with a ribosomal RNA production rate of about 7%/day, despite the slow division rates observed in concurrent DNA-labeling analysis. Similarly, in 2 patients with malignant infiltration of lymph nodes, administration of [6,6-(2)H(2)]-glucose (by intravenous infusion for 24 h) before excision biopsy allowed estimation of DNA and RNA turnover in lymph node samples. CONCLUSIONS: Our study results demonstrate the proof-of-principle that deuterium-labeled glucose may be used to analyze RNA turnover, in addition to DNA production/cell proliferation, in clinical samples. [less ▲]

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See detailMeasurement of Right Ventricular Ejection Fraction and Volume by the Thermodilution Technique in the Unsedated Calf
Amory, Hélène ULg; Mc Entee, Kathleen ULg; Linden, Annick ULg et al

in Research in Veterinary Science (1994), 56(1), 75-81

This study was designed to test the applicability and the reproducibility of the thermodilution method in the measurement of right ventricular ejection fraction (EF), end-diastolic volume (EDV) and end ... [more ▼]

This study was designed to test the applicability and the reproducibility of the thermodilution method in the measurement of right ventricular ejection fraction (EF), end-diastolic volume (EDV) and end-systolic volume (ESV) in unsedated cattle. In the 16 healthy calves studied, it was possible, using a fast response thermodilution catheter, to obtain characteristic exponential steplike washout curves, allowing the calculation of right ventricular EF, EDV and ESV. Five to 10 successive thermodilution curves were recorded within a few minutes and two to five similar sets of measurements were performed throughout the same day to test the reproducibility of the technique. The same protocol was repeated the following day. Right ventricular EF, EDV and ESV were reproducible successively, throughout a given day and on following days. The mean intraset, intraday and interdays coefficients of variation ranged from a mean of 4.0 (SD 4.1) per cent to a mean of 18.2 (SD 7.9) per cent. Values of right ventricular volumes and EF agreed with those previously reported in various animal species. Thermodilution was, therefore, shown to be a useful method for measuring with a satisfactory reproducibility, right ventricular EF, EDV and ESV in the unsedated healthy calf [less ▲]

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See detailMeasurement of selected halogenated contaminants in human serum and milk using GCxGC-IDTOFMS
Focant, Jean-François ULg; sjodin, Andreas; Turner, Wayman et al

in Organohalogen Compounds (2004)

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See detailMeasurement of selected polybrominated diphenyl ethers, polybrominated and polychlorinated biphenyls, and organochlorine pesticides in human serum and milk using comprehensive two-dimensional gas chromatography isotope dilution time-of-flight mass spectrometry
Focant, Jean-François ULg; Sjodin, Andreas; Turner, Wayman E. et al

in Analytical Chemistry (2004), 76(21), 6313-6320

A new method using comprehensive two-dimensional gas chromatography and isotope dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) for the simultaneous measurement of selected polychlorinated ... [more ▼]

A new method using comprehensive two-dimensional gas chromatography and isotope dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) for the simultaneous measurement of selected polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and brominated flame retardants is presented. In contrast to the reference methods based on classical GC/MS, a single injection of the extract containing all compounds of interest results in accurate identification and quantification. Using GC x GC ensures the chromatographic separation of most compounds, and TOFMS allows mass spectral deconvolution of coeluting compounds as well as the use of C-13-labeled internal standards for quantification. Isotope ratio measurements of the most intense ions for both native and labels ensure the required specificity. The use of this new method with an automated sample preparation procedure developed at the Centers for Disease Control and Prevention (CDC) for the analysis of human serum and milk compared favorably to conventional isotope-dilution one-dimensional gas chromatography-high-resolution mass spectrometty (GC-IDHRMS) for the different human serum and milk pools tested. The instrumental detection limits ranged between 0.5 pg/muL and 10 pg/muL and the method detection limits ranged between I and 15 pg/muL (N = 59 analytes). The reproducibility of the method was almost as good as with GC-IDHRMS, the relative standard deviations ranging between 1 and 11% for OCPs measured in human serum. OCP, PBDE, and PCB levels measured using the two methods were highly correlated, and the deviations between the two methods were below 20% for most analytes with concentrations above 1 ng/g milk lipids. [less ▲]

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See detailMeasurement of serum albumin by protein electrophoresis and bromocresol green methods in canine and equine patients
Ramery, Eve ULg; Bureau, Fabrice ULg

in Veterinary Clinical Pathology (2012, December), 41(4), 5

Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported ... [more ▼]

Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported. However, in human medicine, at low albumin and high globulin concentrations, BCG also binds to α- and β-globulins fractions. Surprisingly, there is no recent literature evaluating the BCG method in diseased domestic animals. Objective: The purpose of the present study was therefore to compare the serum albumin values obtained by SPE and BCG in canine and equine patients. Methods: Albumin measured by BCG and SPE was compared in the serum from 30 canine and 30 equine patients. Results: Good correlation was found (Spearman´s rho rs = 0.91 in dogs and 0.84 in horses). However, Altman and Bland analysis of results showed a positive bias of BCG with SPE (3.41 g/l in horses and 0.41 g/l in dogs) that increased when only hypoalbuminemic patients were considered (4.17 g/l in horses and 1.31 g/l in dogs). Conclusion: Results confirm that, despite good correlation, BCG method tends to overestimate albumin, especially in hypoalbuminemic patients. This is particularly evident in horses. [less ▲]

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See detailMeasurement of shrinkage and cracks associated to convective drying of soft materials by X-ray microtomography
Léonard, Angélique ULg; Blacher, Silvia ULg; Marchot, Pierre ULg et al

in Drying Technology (2004), 22(7), 1695-1708

Traditionally, the measurement of shrinkage occurring during drying is performed by destructive or poorly accurate techniques such as volume displacement methods. Cracks detection and quantification are ... [more ▼]

Traditionally, the measurement of shrinkage occurring during drying is performed by destructive or poorly accurate techniques such as volume displacement methods. Cracks detection and quantification are realised either by destructive techniques or sophisticated but expensive nondestructive ones (NMR imaging). X-ray microtomography in combination with image analysis provides an accurate, nondestructive and easy to use technique to determine simultaneously shrinkage and crack extent. Results reported in this article concern drying of wastewater sludges whose management will become a real challenge in the years to come. These results show a clear relation between drying kinetics and crack development. This could be related to the development of internal diffusional limitations inducing moisture gradients and mechanical stresses leading to cracks formation. [less ▲]

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