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See detailmbmdr: an R package for exploring gene-gene interactions associated with binary or quantitative traits
Calle, M. Luz; Urrea, Victor; Van Steen, Kristel ULg

in Bioinformatics (2010), 26(17), 2198-2199

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See detailMBO en Aandeelhouder
Corhay, Albert ULg; Herst, Arthur; Tourani Rad, Alireza

in Bedrifskunde (1994), 4

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See detailMcCune-Albright Syndrome: A Detailed Pathological And Genetic Analysis of Disease Effects in an Adult Patient.
Vasilev, Vladimir; Daly, Adrian ULg; THIRY, Albert ULg et al

in The Journal of clinical endocrinology and metabolism (2014)

Context: McCune Albright syndrome (MAS) is a clinical association of endocrine and non-endocrine anomalies caused by post-zygotic mutation of the GNAS1 gene, leading to somatic activation of the ... [more ▼]

Context: McCune Albright syndrome (MAS) is a clinical association of endocrine and non-endocrine anomalies caused by post-zygotic mutation of the GNAS1 gene, leading to somatic activation of the stimulatory alpha subunit of G protein (Gsalpha). Important advances have been made recently in describing pathological characteristics of many MAS-affected tissues, particularly pituitary, testicular and adrenal disease. Other rarer disease related features are emerging. Objective: To study pathological and genetic findings of MAS on a tissue-by-tissue basis in classically and non classically affected tissues. Design: A comprehensive autopsy and genetic analysis Setting: Tertiary referral University Hospital Patients: Adult male patient with MAS and severe disease burden including gigantism Intervention(s): Clinical, hormonal and radiographic studies; gross and microscopic pathology analyses, conventional PCR and droplet digital PCR analyses of affected and non affected tissues Main Outcome Measure: Pathological findings, presence of GNAS1 mutations Results: The patient was diagnosed with MAS syndrome at six years of age based on the association of cafe-au-lait spots and radiological signs of polyostotic fibrous dysplasia. Gigantism developed and hyperprolactinemia, hypogonadotropic hypogonadism and hyperparathyroidism were diagnosed throughout adult period. The patient died at the age of 39 from pulmonary embolism. A detailed study revealed mosaiscism for the p.R201C GNAS mutation distributed across many endocrine and non-endocrine tissues. These genetically implicated tissues included rare or previously undescribed disease associations including primary hyperparathyroidism, and hyperplasia of the thymus and endocrine pancreas. Conclusions: This comprehensive pathological study of a single patient highlights the complex clinical profile of MAS and illustrates important advances in understanding the characteristics of somatic GNAS1 related pathology across a wide range of affected organs. [less ▲]

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See detailMCDA applied to the architectural sketch design: an aid to conception of the building envelope
Colson, Gérard ULg; Hauglustaine, Jean-Marie ULg

in Papiers de recherche / Working papers - Faculté d'Economie, de Gestion et de Sciences sociales (2000), (G.E.M.M.E n°0008), 13

In the conception of the building envelope, the sketch design is a crucial moment of the architectural design. The first drawing gives a concrete expression to the project author's first choices regarding ... [more ▼]

In the conception of the building envelope, the sketch design is a crucial moment of the architectural design. The first drawing gives a concrete expression to the project author's first choices regarding a set of requirements and preferences, as expressed by the multiple stakholders of the project, namely: the building owner, users, public authorities and the design team surrounding the architect. The present study explores a Multiple Criteria Decision Aid procedure (MCDA), an aid to help stakeholders improve this stage of the sketch design. The methodology is built close to the usual design process. This paper presents design and decision aid intertwined procedures and briefly describes the softwares involed: AMCE and EsQUIsE. The decision aid process is a first attempt to address the architectural sketch design challenge. The reader is expected to react to the suggested presentation of this still open MCDA problem. [less ▲]

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See detailMCDA applied to the architectural sketch design: an aid to conception of the building envelope
Colson, Gérard ULg; Hauglustaine, Jean-Marie ULg

in Colorni, A.; Paruccini, M.; Roy, B. (Eds.) A-MCD-A - Aide Multicritère à la Décision - Multiple Criteria Decision Aiding (2001)

In the conception of the building envelope, the sketch design is a crucial moment of the architectural design. The first drawing gives a concrete expression to the project author's first choices regarding ... [more ▼]

In the conception of the building envelope, the sketch design is a crucial moment of the architectural design. The first drawing gives a concrete expression to the project author's first choices regarding a set of requirements and preferences, as expressed by the multiple stakeholders of the project, namely: the building owner, users, public authorities and the design team surrounding the architect. The present study explores a Multiple Criteria Decision Aid procedure (MCDA), an aid to help stakeholders improve this stage of the sketch design. The methodology is built close to the usual design process. This paper presents design and decision aid intertwined procedures and briefly describes the softwares involved: AMCE and EsQUIsE. The decision aid process is a first attempt to address this architectural sketch design challenge. The reader is expected to react to the suggested presentation of this still open MCDA problem. [less ▲]

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See detailMCF-7/BOS cells membrane proteome: comparison of two isolation methods using mass spectrometry
Bertrand, Virginie ULg; Massart, Anne-Cécile ULg; De Pauw-Gillet, Marie-Claire ULg et al

Poster (2009)

Membrane proteins play an important role in biological processes but their isolation and quantification using classical techniques is often limited due to their poor solubility and relatively low ... [more ▼]

Membrane proteins play an important role in biological processes but their isolation and quantification using classical techniques is often limited due to their poor solubility and relatively low abundance. These membrane markers have to be accessible to antibodies and should be potential therapeutic targets. The tests were conducted on MCF-7 / BOS cell line, immortal and easier to cultivate. The goal of this work is to obtain a pure membrane fraction to facilitate the analysis of the sample. To isolate transmembrane proteins, we compared two methods. The first one used different extraction cycles characterized by different buffers to isolate membrane proteins. The second method labelled accessible extracellular domains at the surface of MCF-7 cells with biotin prior to differential centrifugation. [less ▲]

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See detailMCF-7/BOS cells membrane proteome: comparison of two isolation methods using mass spectrometry
Bertrand, Virginie ULg; Massart, Anne-Cécile ULg; De Pauw-Gillet, Marie-Claire ULg et al

Poster (2009)

Membrane proteins (MP) play an important role in biological processes. Isolation and quantification of these MP using classical techniques is often limited due to their poor solubility and relatively low ... [more ▼]

Membrane proteins (MP) play an important role in biological processes. Isolation and quantification of these MP using classical techniques is often limited due to their poor solubility and relatively low abundance. These membrane proteins enclosed markers which could be potential therapeutic targets. These potential therapeutic targets have to be accessible to antibodies and need to be presented in the plasmic membrane. Assays were conducted on MCF-7 / BOS cell line, immortal and easier to cultivate. The goal of this work is to obtain an enriched membrane fraction to facilitate the analysis of the sample and to simplify the complex proteins mixture. To isolate transmembrane proteins, we compared two methods. The first one used different extraction cycles characterized by different buffers to isolate membrane proteins. The second method labelled accessible extracellular domains at the surface of MCF-7 cells with biotin prior to differential centrifugation. The obtained enriched membrane proteome was digested with trypsin and/or Lysyl Endopeptidase. Obtained peptides were separated by 2D-HPLC chromatography and on-line analysed ion trap mass spectrometer, the Esquire HCT. [less ▲]

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See detailMCI conversion to dementia and the APOE genotype : A prediction study with FDG-PET
Mosconi, L.; Perani, D.; Sorbi, S. et al

in Neurology (2004), 63(12), 2332-2340

Objectives: To investigate whether the combination of fluoro-2-deoxy-D-glucose (FDG) PET measures with the APOE genotype would improve prediction of the conversion from mild cognitive impairment (MCI) to ... [more ▼]

Objectives: To investigate whether the combination of fluoro-2-deoxy-D-glucose (FDG) PET measures with the APOE genotype would improve prediction of the conversion from mild cognitive impairment (MCI) to Alzheimer disease ( AD). Method: After 1 year, 8 of 37 patients with MCI converted to AD (22%). Differences in baseline regional glucose metabolic rate (rCMRglc) across groups were assessed on a voxel-based basis using a two-factor analysis of variance with outcome (converters [n = 8] vs nonconverters [n = 29]) and APOE genotype (E4 carriers [E4+] [n = 16] vs noncarriers [E4-] [n = 21]) as grouping factors. Results were considered significant at p < 0.05, corrected for multiple comparisons. Results: All converters showed reduced rCMRglc in the inferior parietal cortex (IPC) as compared with the nonconverters. Hypometabolism in AD-typical regions, that is, temporoparietal and posterior cingulate cortex, was found for the E4+ as compared with the E4- patients, with the E4+/converters (n = 5) having additional rCMRglc reductions within frontal areas, such as the anterior cingulate (ACC) and inferior frontal (IFC) cortex. For the whole MCI sample, IPC rCMRglc predicted conversion to AD with 84% overall diagnostic accuracy (p = 0.003). Moreover, ACC and IFC rCMRglc improved prediction for the E4+ group, yielding 100% sensitivity, 90% specificity, and 94% accuracy (p < 0.0005), thus leading to an excellent discrimination. Conclusion: Fluoro-2-deoxy-D-glucose-PET measures may improve prediction of the conversion to Alzheimer disease, especially in combination with the APOE genotype. [less ▲]

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See detailMcLuhan, trente ans après
Durand, Pascal ULg

Scientific conference (1999, December 17)

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See detailMcLuhan, trente ans après
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (1999), 37

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See detailMcLuhan, trente ans après
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (1999), 37

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See detailMDM2 restrains estrogen-mediated AKT activation by promoting TBK1-dependent HPIP degradation.
Shostak, Kateryna ULg; Patrascu, F.; Göktuna, Serkan ULg et al

in Cell death and differentiation (2014), 5(21), 811-24

Restoration of p53 tumor suppressor function through inhibition of its interaction and/or enzymatic activity of its E3 ligase, MDM2, is a promising therapeutic approach to treat cancer. However, because ... [more ▼]

Restoration of p53 tumor suppressor function through inhibition of its interaction and/or enzymatic activity of its E3 ligase, MDM2, is a promising therapeutic approach to treat cancer. However, because the MDM2 targetome extends beyond p53, MDM2 inhibition may also cause unwanted activation of oncogenic pathways. Accordingly, we identified the microtubule-associated HPIP, a positive regulator of oncogenic AKT signaling, as a novel MDM2 substrate. MDM2-dependent HPIP degradation occurs in breast cancer cells on its phosphorylation by the estrogen-activated kinase TBK1. Importantly, decreasing Mdm2 gene dosage in mouse mammary epithelial cells potentiates estrogen-dependent AKT activation owing to HPIP stabilization. In addition, we identified HPIP as a novel p53 transcriptional target, and pharmacological inhibition of MDM2 causes p53-dependent increase in HPIP transcription and also prevents HPIP degradation by turning off TBK1 activity. Our data indicate that p53 reactivation through MDM2 inhibition may result in ectopic AKT oncogenic activity by maintaining HPIP protein levels.Cell Death and Differentiation advance online publication, 31 January 2014; doi:10.1038/cdd.2014.2. [less ▲]

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See detailMDRD equation for GFR estimation : analytical limitations
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Krzesinski, Jean-Marie ULg et al

Conference (2007, March 23)

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See detailMDRD or CKD-EPI study equations for estimating prevalence of stage 3 CKD in epidemiological studies: which difference? Is this difference relevant?
Delanaye, Pierre ULg; Cavalier, Etienne ULg; Mariat, C. et al

in BMC Nephrology (2010), 11(8),

Background: Prevalence of stage 3 chronic kidney disease (CKD) is increasing according to the NHANES study. Prevalence has been calculated using the MDRD study equation for estimating glomerular ... [more ▼]

Background: Prevalence of stage 3 chronic kidney disease (CKD) is increasing according to the NHANES study. Prevalence has been calculated using the MDRD study equation for estimating glomerular filtration rate (GFR). Recently, a new estimator based on creatinine, the CKD-EPI equation, has been proposed which is presumed to better perform in normal GFR ranges. The aim of the study was to measure the difference in prevalence of stage 3 CKD in a population using either the MDRD or the CKD-EPI study equations. Methods: CKDscreening is organized in the Province of Liège, Belgium. On a voluntary basis, people aged between 45 and 75 years are invited to be screened. GFR is estimated by the MDRD study equation and by the "new" CKD-EPI equations. Results: The population screened consisted in 1992 people (47% of men). Mean serum creatinine was 0.86 ± 0.20 mg/dl. The prevalence of stage 3 CKD in this population using the MDRD or the CKD-EPI equations was 11.04 and 7.98%, respectively. The prevalence of stage 3 CKD is significantly higher with the MDRD study equation (p <0,0012). Conclusions: Prevalence of stage 3 CKDvaries strongly following the method used for estimating GFR, MDRD or CKDEPI study equations. Such discrepancies are of importance and must be confirmed and explained by additional studies using GFR measured with a reference method. [less ▲]

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See detailMDRD versus CKD-EPI equation to estimate glomerular filtration rate in kidney transplant recipients
Masson, Ingrid; Flamant, Martin; Maillard, Nicolas et al

in Transplantation (2013), 95(10), 1211-1217

Background. The new Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) creatinine-based equation <br />was developed to address the systematic underestimation of the glomerular filtration rate ... [more ▼]

Background. The new Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) creatinine-based equation <br />was developed to address the systematic underestimation of the glomerular filtration rate (GFR) by the Modification <br />of Diet in Renal Disease (MDRD) Study equation in patients with a relatively well-preserved kidney function. The <br />performance of the new equation for kidney transplant recipients is discussed. <br />Methods. We analyzed the performances of the CKD-EPI equation in comparison with the MDRD Study equation in <br />825 stable kidney transplant recipients. Bias, precision, and accuracy within 30% of true GFR were determined. GFR <br />was measured by urinary clearance of inulin (n=488) and plasma clearance of 51Cr-EDTA (n=337). <br />Results. Mean measured GFR (mGFR) was 50T19 mL/min/1.73 m2. On the whole cohort, bias was significantly lower <br />for MDRD Study equation compared with CKD-EPI creatinine. This superiority translates into a better accuracy <br />(80% and 74% for the MDRD and CKD-EPI creatinine, respectively). The best performance of the MDRD Study <br />equation is confirmed both in the subgroups of patients with mGFR G60 mL/min/1.73 m2 and between 60 and <br />90 mL/min/1.73 m2. For mGFR 990 mL/min/1.73 m2, there were no significant differences between the two <br />equations in terms of performance. <br />Conclusions. The CKD-EPI creatinine equation does not offer a better GFR prediction in renal transplant patients <br />compared with the MDRD Study equation, even in the earlier CKD stages. [less ▲]

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See detailMDRD VERSUS CKD-EPI EQUATIONS TO ESTIMATE GLOMERULAR FILTRATION RATE IN OBESE PATIENTS
BOUQUEGNEAU, Antoine ULg; CAVALIER, Etienne ULg; Krzesinski, Jean-Marie ULg et al

in Nephrology Dialysis Transplantation (2013, April 18), 28(supplément 1), 117-139

Introduction and Aims: Obesity is recognized as a risk factor both for the development and progression of chronic kidney disease (CKD). Estimating glomerular filtration rate (GFR) is thus especially ... [more ▼]

Introduction and Aims: Obesity is recognized as a risk factor both for the development and progression of chronic kidney disease (CKD). Estimating glomerular filtration rate (GFR) is thus especially important to follow these patients. We have tested the performances of two creatinine-based equations, namely the MDRD and CKD-EPI equations, in an obese population. Conclusions :Both in the global and subgroup analyses, the CKD-EPI equation did not outperform the MDRD study equation. The performances of both equations were worse in CKD patients. These two conclusions were still valid if indexed GFR was considered. [less ▲]

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See detailMe, Myself and I
Dejasse, Erwin ULg

in Anny Servais (2004)

L'autoportrait dans la peinture d'Anny Servais

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See detail"ME, MYSELF AND TED" : Réflexions sur l'intégration des Technologies Educatives aux pratiques professionnelles des enseignants
Van de Poël, Jean-François ULg; Verpoorten, Dominique ULg

Scientific conference (2014, November 05)

Cette conférence aborde les principaux courants utiles et les modèles qui sous-tendent l'usage des technologies éducatives dans la pratique de l'enseignant dans le supérieur.

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See detailMealworms: Alternate Source of Lipids
Danthine, Sabine ULg; Blecker, Christophe ULg; Paul, Aman ULg et al

Scientific conference (2014, May 16)

The aim of present study was to determine the physicochemical properties of the oil obtained from Tenebrio molitor larvae (mealworms) and explore its potential as edible oil. Five batches of Tenebrio ... [more ▼]

The aim of present study was to determine the physicochemical properties of the oil obtained from Tenebrio molitor larvae (mealworms) and explore its potential as edible oil. Five batches of Tenebrio molitor larvae were investigated for their lipid content and physiochemical properties. Three batches were reared in lab (3 different productions) and two were purchased from a local supplier. The lipids were extracted using a cold extraction technique employing 2:1 ratio chloroform/methanol as solvent. The fatty acid profile was determined using gas chromatography and triacylglycerol profile using HPLC. The thermal properties of the lipid extracts were also analyzed using differential scanning calorimetry. All the samples contained high amount of unsaturated fatty acids. The chemical composition and the thermal properties of the samples varied with the source. With this quantity and quality of lipid content, mealworms offer potential as an important source of edible lipids. [less ▲]

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See detailMean Expression of the X-Chromosome is Associated with Neuronal Density.
Swingland, James T.; Durrenberger, Pascal F.; Reynolds, Richard et al

in Frontiers in Neuroscience (2012), 6

Background: Neurodegenerative diseases are characterized by key features such as loss of neurons, astrocytosis, and microglial activation/proliferation. These changes cause differences in the density of ... [more ▼]

Background: Neurodegenerative diseases are characterized by key features such as loss of neurons, astrocytosis, and microglial activation/proliferation. These changes cause differences in the density of cell types between control and disease subjects, confounding results from gene expression studies. Chromosome X (ChrX) is known to be specifically important in the brain. We hypothesized the existence of a chromosomal signature of gene expression associated with the X-chromosome for neurological conditions not normally associated with that chromosome. The hypothesis was investigated using publicly available microarray datasets from studies on Parkinson's disease, Alzheimer's disease, and Huntington's disease. Data were analyzed using Chromowave, an analytical tool for detecting spatially extended expression changes along chromosomes. To examine associations with neuronal density and astrocytosis, the expression of cell specific reporter genes was extracted. The association between these genes and the expression patterns extracted by Chromowave was then analyzed. Further analyses of the X:Autosome ratios for laser dissected neurons, microglia cultures and whole tissue were performed to detect cell specific differences. Results: We observed an extended pattern of low expression of ChrX consistent in all the neurodegenerative disease brain datasets. There was a strong correlation between mean ChrX expression and the pattern extracted from the autosomal genes representing neurons, but not with mean autosomal expression. No chromosomal patterns associated with the neuron specific genes were found on other chromosomes. The chromosomal expression pattern was not present in datasets from blood cells. The X:Autosome expression ratio was also higher in neuronal cells than in tissues with a mix of cell types. Conclusions: The results suggest that neurological disorders show as a reduction in mean expression of many genes along ChrX. The most likely explanation for this finding relates to the documented general up-regulation of ChrX in brain tissue which, this work suggests, occurs primarily in neurons. If validated, this cell specific ChrX expression warrants further research as understanding the biological reasons and mechanisms for this expression, may help to elucidate a connection with the development of neurodegenerative disorders. [less ▲]

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