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See detailManipulations chromosomiques. Amélioration génétique et contrôle du sexe chez les poissons.
Castelli M.; Mélard, Charles ULg; Poncin, Pascal ULg et al

in Nouvelles de la Science et des Technologies (1990), 8(2), 61-71

Detailed reference viewed: 49 (8 ULg)
See detailLes manipulations des médias
Geuens, Geoffrey ULg

Conference given outside the academic context (2001)

Atelier d'éducation aux médias

Detailed reference viewed: 21 (11 ULg)
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See detailMannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes is mediated by the CD18 subunit of beta2-integrins
Fett, Thomas ULg; Zecchinon, Laurent ULg; Vanden Bergh, Philippe ULg et al

in Microbial Pathogenesis (2008), 45

Mannheimiosis is the major respiratory disease among some ruminants, whereas it is not pathogenic for other mammals, an observation that has been attributed to a specific interaction between Mannheimia ... [more ▼]

Mannheimiosis is the major respiratory disease among some ruminants, whereas it is not pathogenic for other mammals, an observation that has been attributed to a specific interaction between Mannheimia haemolytica leukotoxin (Lkt) and bovine or ovine CD18 subunit of lymphocyte function-associated antigen-1 (LFA-1) and Mac-1. We therefore hypothesized that Lkt utilizes CD18 as its receptor on caprine leukocytes as well. We have transiently transfected the b2-integrins-deficient K-562 cell line with cDNAs encoding caprine CD11a and caprine CD18 to determine the susceptibility of the transfectants to Lktinduced cytolysis. Flow cytometric analysis of the transfectants revealed surface expression of caprine LFA-1 and lysis by Lkt in a concentration-dependent manner whereas the parent cells were not. Moreover, K562 cells expressing caprine CD18 and human or bovine CD11a were also sensitive to Lkt whereas K-562 cells expressing caprine CD11a and human CD18 were not. Taken together, these results indicate that CD18 on caprine leukocytes serves as a receptor for Lkt. [less ▲]

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See detailLa mannheimiose : d’une liaison (moléculaire) fatale à une des principales maladies d’élevage des ruminants
Fett, Thomas ULg; Zecchinon, Laurent; Vanden Bergh, Philippe et al

in Annales de Médecine Vétérinaire (2009), 153(1), 31-53

Les pneumonies bactériennes constituent un problème majeur dans l'élevage et l'engraissement des bovins, avec des répercussions très élevées en termes de morbidité et de mortalité. Parmi les différentes ... [more ▼]

Les pneumonies bactériennes constituent un problème majeur dans l'élevage et l'engraissement des bovins, avec des répercussions très élevées en termes de morbidité et de mortalité. Parmi les différentes causes biologiques recensées, Mannheimia haemolytica tient le haut de l’affiche puisqu’elle est connue comme agent pathogène compliquant principal, voire systématique. Une de ses particularités est qu’elle ne déclenche de pneumonie broncho-alvéolaire fibrino-hémorragique que chez les ruminants. Les lésions observées résultent d’une nécrose en masse des neutrophiles et macrophages et du relarguage de leurs différents constituants. Au niveau moléculaire, cette spécificité trouve son origine dans l’intimité de la liaison entre la leucotoxine, le facteur de virulence majeur de la bactérie, et la sous-unité CD18 des récepteurs de surface leucocytaires bêta2-intégrines. La revue décrit cette liaison et ses conséquences aux niveaux macroscopique et microscopique, avant de faire le point sur les dernières avancées de la recherche et d’ébaucher quelques perspectives thérapeutiques. [less ▲]

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See detailMannosylated amphiphilic and degradable PEO-b-PCL copolymers for drug delivery systems: preparation and sugar availability characterizations
Freichels, Hélène ULg; Imberty, Anne; Auezély-Velty, Rachel et al

Poster (2009, May 14)

Over the last decade, polymer micelles and nanoparticles attracted an increasing interest in pharmaceutical research because they can be used as efficient drug delivery systems. In this field, amphiphilic ... [more ▼]

Over the last decade, polymer micelles and nanoparticles attracted an increasing interest in pharmaceutical research because they can be used as efficient drug delivery systems. In this field, amphiphilic copolymers combining poly(ethylene oxide) and aliphatic polyester (such as poly(ε-caprolactone) (PCL) or polylactide (PLA)) are particularly of interest because (i) PEO has unique protein- repellent properties and thus provides a stealth behaviour to the drug carriers and (ii) aliphatic polyesters are biocompatible and biodegradable hydrophobic matrices well-suited for the incorporation of an hydrophobic drug. By end-capping the hydrophilic segment by a targeting moiety so that they may interact with membrane receptors, the biodistribution of polymeric micelles and nanoparticles stabilized with this copolymer can be modulated and can induce specific cellular uptake by receptor-mediated endocytosis. One class of interesting targeting agent is the saccharides, in particular the mannose, because of its specific interaction with mannose receptor, which are found on peripheral and bone marrow macrophages, dendritic cells and sinusoidal liver cells. In this study, the reductive amination reaction is use to attach this targeting agent. After optimisation of the reaction with amino fluorescein, a model amine, mannosylated copolymer of PEO and PCL has been prepared. The surface availability of the saccharide upon the micelles in aqueous phosphate buffer was then assessed by DLS through binding with the protein Concanavalin A (ConA), a known mannose receptor. The interactions between the Bcla lectin and the mannosylated micelles have then been studied by Isothermal Titration Calorimetry (ITC) and the thermodynamic parameters have been obtained. This polymer is particularly useful for the stabilization of PLGA nanoparticles with the goal to target M cells for oral vaccination. [less ▲]

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See detailMannosylated poly(ethylene oxide)-b-Poly(epsilon-caprolactone) diblock copolymers: Synthesis, characterization, and interaction with a bacterial lectin
Rieger, Jutta ULg; Stoffelbach, François; Cui, Di et al

in Biomacromolecules (2007), 8(9), 2717-2725

A novel bioeliminable amphiphilic poly(ethylene oxide)-b-poly(epsilon-caprolactone) (PEO-b-PCL) diblock copolymer end-capped by a mannose residue was synthesized by sequential controlled polymerization of ... [more ▼]

A novel bioeliminable amphiphilic poly(ethylene oxide)-b-poly(epsilon-caprolactone) (PEO-b-PCL) diblock copolymer end-capped by a mannose residue was synthesized by sequential controlled polymerization of ethylene oxide and epsilon-caprolactone, followed by the coupling of a reactive mannose derivative to the PEO chain end. The anionic polymerization of ethylene oxide was first initiated by potassium 2-dimethylaminoethanolate. The ring-opening polymerization of epsilon-caprolactone was then initiated by the omega-hydroxy end-group of PEO previously converted into an Al alkoxide. Finally, the saccharidic end-group was attached by quaternization of the tertiary amine (alpha-end-group of the PEO-b-PCL with a brominated mannose derivative. The copolymer was fully characterized in terms of chemical composition and purity by high-resolution NMR spectroscopy and size exclusion chromatography. Furthermore, measurements with a pendant drop tensiometer showed that both the mannosylated copolymer and the non-mannosylated counterpart significantly decreased the dichloromethane/water interfacial tension. Moreover, these amphiphilic copolymers formed monodisperse spherical micelles in water with an average diameter of similar to 11 nin as measured by dynamic light scattering and cryo-transmission electron microscopy. The availability of mannose as a specific recognition site at the surface of the micelles was proved by isothermal titration microcalorimetry (ITC), using the BclA lectin (from Burkholderia cenocepacia), which interacts selectively with a-D-mannopyranoside derivatives. The thermodynamic parameters of the lectin/mannose interaction were extracted from the ITC data. These colloidal systems have great potential for drug targeting and vaccine delivery systems. [less ▲]

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See detailManoeuvrability of ships in shallow and restricted waters
Marchal, Jean ULg

Conference (1993, September)

Detailed reference viewed: 22 (4 ULg)
See detailManoscritti teatrali della Biblioteca Riccardiana di Firenze.
Moreno, Paola ULg

in Scriptorium (1999)

Detailed reference viewed: 17 (0 ULg)
See detailIl manoscritto di Santa Caterina
Deweert, Willy; Ricciardi, Stefania ULg

Book published by Rizzoli (2011)

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See detailLe manque de généralisation chez les enfants dysphasiques : une étude longitudinale
Leroy, Sandrine ULg; Parisse, Christophe; Maillart, Christelle ULg

in ANAE : Approche Neuropsychologique des Apprentissages chez l'Enfant (2014), 26(131), 357-365

Usage-based theories postulate that development of linguistic system depends on a gradual generalization mechanism during which use of lexicalized language forms are little-by-little replaced by use of ... [more ▼]

Usage-based theories postulate that development of linguistic system depends on a gradual generalization mechanism during which use of lexicalized language forms are little-by-little replaced by use of abstract construction schemas. Several studies have shown limited morphosyntactic productivity in children with specific language impairment (SLI). In this longitudinal study, we have tested hypothesis according to which generalization would be hindered in children with SLI. [less ▲]

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See detailLe manque de variabilité des schémas de construction comme facteur explicatif des difficultés morphosyntaxiques chez les enfants dysphasiques
Leroy, Sandrine ULg; Moulin, Mélissa; Parisse, Christophe et al

Conference (2010, July 06)

Detailed reference viewed: 81 (6 ULg)
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See detailA mantle-derived parent massif anorthosites
Longhi, John; Vander Auwera, Jacqueline ULg

Conference (1994)

Detailed reference viewed: 1 (0 ULg)
See detailA manual of advanced celestial photography -- Brad D. Wallis et Robert W. Provin
Manfroid, Jean ULg

in Ciel et Terre (1989), 105

Detailed reference viewed: 31 (0 ULg)
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See detailManuel d'analyse qualitative. Analyser sans compter ni classer
Lejeune, Christophe ULg

Book published by De Boeck (2014)

La méthode qualitative s'est dotée d'outils et de techniques : journal de bord, propriété, dimension, catégorie, échantillonnage, saturation, scénario, mémo et compte rendu, codage (ouvert, axial et ... [more ▼]

La méthode qualitative s'est dotée d'outils et de techniques : journal de bord, propriété, dimension, catégorie, échantillonnage, saturation, scénario, mémo et compte rendu, codage (ouvert, axial et sélectif). Cet ouvrage présente le rôle respectif de chacun de ces éléments et permet à tous de s'approprier la logique qui les sous-tend. S'appuyant sur des exemples issus de recherches réelles, il expose avec clarté comment conduire sa propre recherche. Il fournit des astuces développées par les praticiens les plus aguerris et permet ainsi au lecteur d'évaluer sa propre avancée dans le monde passionnant de la recherche qualitative. [less ▲]

Detailed reference viewed: 435 (104 ULg)