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See detailMolecular Systematics of Rattini in South East Asia
Pagès, Marie ULg

Conference (2008, October)

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See detailMolecular targeting of antiangiogenic factor 16K hPRL inhibits oxygen-induced retinopathy in mice
Pan, H.; Nguyen, Ngoc-Quynh-Nhu ULg; Yoshida, H. et al

in Investigative Ophthalmology & Visual Science (2004), 45(7), 2413-2419

PURPOSE. To examine the ability and mechanism of the 16 kDa N-terminal fragment of human prolactin (16K hPRL) in the inhibition of abnormal retinal neovascularization. METHODS. The 16K hPRL-encoding ... [more ▼]

PURPOSE. To examine the ability and mechanism of the 16 kDa N-terminal fragment of human prolactin (16K hPRL) in the inhibition of abnormal retinal neovascularization. METHODS. The 16K hPRL-encoding sequence was inserted into an adenoviral vector (16K-Ad). Western blot analysis verified the expression of 16K hPRL and inhibition of proliferation, confirming functional activity of the 16K hPRL in virus-infected adult bovine aortic endothelial (ABAE) cells. 16K hPRL inhibited retinal neovascularization in a mouse model of oxygen-induced retinopathy. The ability of recombinant 16K hPRL expressed in E. coli (r16K hPRL) was compared to that of endostatin in inducing apoptosis of cultured human retinal endothelial cells (HREC). RESULTS. 16K was expressed in virus-infected ABAE cells and resulted in a dose-dependent inhibition of cell proliferation. Eyes injected with 16K-Ad showed a reduction in preretinal neovascularization of 82.3 +/- 9.3% (P < 0.00001) when compared to uninjected controls. r16K hPRL was 100 times more potent than endostatin in inducing apoptosis in HRECs. CONCLUSIONS. Intravitreal administration of 16K hPRL inhibited neovascularization in the mouse model of oxygen-induced retinopathy. 16K hPRL stimulated apoptosis in HRECs and inhibited cell proliferation in ABAE cells. These results suggested a potential therapeutic role for 16K hPRL in the treatment of proliferative retinopathies. [less ▲]

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See detailMolecular testing of multiple HIV-1 transmissions in a criminal case
Lemey, Philippe; Van Dooren, Sonia; Van Laethem, Kristel et al

in AIDS (2005), 19(15), 1649-1658

Objective: To test the a priori hypothesis of HIV-1 transmission from one suspect to six recipients in a criminal case. Methods: Partial pol and/or env sequences were obtained for at least two samples of ... [more ▼]

Objective: To test the a priori hypothesis of HIV-1 transmission from one suspect to six recipients in a criminal case. Methods: Partial pol and/or env sequences were obtained for at least two samples of the suspect and the victims. Appropriate local controls were sampled based on epidemiological and subtype criteria. Phylogenetic testing was performed using different reconstruction methods. Results: Phylogenetic analyses consistently inferred a monophyletic cluster for the suspect and victim samples in both genome regions. This was highly supported by parametric and non-parametric bootstrapping techniques. Moreover, the controls most closely related to the suspect-victim cluster had a similar geographical origin to the suspect. Conclusions: Taking into account the limitations on the conclusions that can be drawn from molecular investigations we could infer that our molecular data is consistent with a scenario of multiple HIV transmission between suspect and victims. [less ▲]

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See detailMOLECULAR TOOLS APPLIED TO THE STUDY OF MICROCYSTIN-PRODUCING CYANOBACTERIA IN BELGIAN WATERBODIES
Lara, Yannick ULg

Doctoral thesis (2014)

‘Blooms’, an increasing worldwide phenomenon, are adversely affecting surface water resource, including reservoirs and lakes, used for drinking water supplies, recreation, crop, irrigation and fisheries ... [more ▼]

‘Blooms’, an increasing worldwide phenomenon, are adversely affecting surface water resource, including reservoirs and lakes, used for drinking water supplies, recreation, crop, irrigation and fisheries. These amenities are affected by recurrent mass proliferations of cyanobacteria. The latter are responsible for the production of a wide range of bioactive compounds, including potent toxins (cyanotoxins). These comprise neurotoxins, cytotoxins, inflammatory agents, and hepatotoxins. Microcystins (MCs), hepatotoxins and tumour promoters are the most documented of the cyanotoxins. The microcystin synthetase gene cluster (mcy) involved in MC biosynthesis consists of a succession of non-ribosomal peptide synthase (NRPS) and polyketide synthetase (PKS) genes. The main producers of MCs are Anabaena, Microcystis, and Planktothrix. However, it is not possible to distinguish a toxic from a non-toxic strain on the basis of their morphology. In the present study, molecular tools were used, optimized and developed to (i) characterize the 16S rRNA gene diversity of planktonic cyanobacteria, (ii) to detect the cyanobacteria responsible for the production of MCs, (iii) to identify the MCs producing taxa, and (iv) to determine the environmental factors that influence the dynamic of toxic and non-toxic genotypes in Belgian freshwaters. Eighty-nine strains were isolated and their 16S rRNA genes sequenced. The 16S rRNA gene diversity was studied in 32 samples by denaturing gradient gel electrophoresis (DGGE). In order to evaluate the contribution of this work to the study of the molecular diversity of cyanobacteria in Belgian waterbodies, 114 (strains and DGGE) sequences obtained during this PhD thesis were compared to Belgian sequences obtained by others. As a result, 14 previously undiscribed operational taxonomic units (OTUs) were found in the present study. For polymerase chain reaction (PCR) detection of the mcyA/B/E genes, the DNA from a total of 162 environmental samples was extracted. The three genes were found together in 64.2% of the samples, whereas the mcyB alone was detected in 95.1% of the samples. In order to identify the mcyE-carriers present in the freshwaters, a restriction fragment length polymorphism (RFLP) was performed on the mcyE gene. The presence of potentially toxic Microcystis was observed in most of the cases.To bypass the constraint of bacterial cultivation, a combination of whole genome amplification (WGA) and enzyme-linked immunoassay (ELISA) was tested on individual colonies of members of two cyanobacteria, Microcystis and Woronichinia, directly from the natural environment. Sequences of 3 different housekeeping genes (ftsZ, gltX, and recA), of 3 mcy genes, and the Internal Transcribed Spacer (ITS) were analyzed for 11 colonies of Microcystis. MCs were detected and quantified by ELISA in 7 of the 11 Microcystis colonies tested, in agreement with the detection of mcy genes. Sequence types (ST) based on the concatenated sequences of housekeeping genes from cyanobacterial colonies from Belgian water bodies appeared to be endemic when compared to those of strains described in the literature. One colony belonged to a yet undiscovered lineage. A similar protocol was used for 6 colonies of the genus Woronichinia, a taxon that is very difficult to cultivate in the laboratory. The 16S rRNA analysis confirmed the colony identification based on morphology. In addition, we obtained for the first time new genetic data for this genus, such as the rpoC1 gene sequences and the sequences and secondary structures of the ITS. The first discovery of NRPS and PKS DNA sequences in Woronichinia colonies highlights the need for further study of this widely occurring genus, to better assess its ability to produce MCs and/or related metabolites. For the first time, in this study, we were able to simultaneously monitor one toxic and one non-toxic genotype of M. aeruginosa using real time qPCR technology during a monitoring of 2 years. Both toxic and non-toxic genotypes dynamics appeared influenced by the photoperiod. In addition, the dynamic of the toxic genotype was influenced of light intensity. The results obtained during this PhD research show the need to characterize toxic cyanobacteria in freshwaters, as well as the conditions that influence MCs concentration dynamics. We showed that factors controlling the dynamics of toxic and non-toxic genotypes are complex. Nevertheless, detection tools can be developed to better understand these widely occurring phenomena. Therefore, efforts should go on in this field with collaborations between the scientists and the authorities. [less ▲]

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See detailMolecular traceability of animals and their products.
Haezebroeck, V.; Renaville, Robert ULg; Bertozzi, C. et al

in Biotechnology in animal husbandry (2001)

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See detailMolecular weight and amino acid composition of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61
Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg; Perkins, Harnold R. et al

in Biochemical Journal (1973), 135(3), 463-468

A procedure allowing the purification of milligram amounts of the exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R61 to protein homogeneity (95% purity) is described. The isolated ... [more ▼]

A procedure allowing the purification of milligram amounts of the exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R61 to protein homogeneity (95% purity) is described. The isolated protein has a molecular weight of about 38000 and consists of one polypeptide chain. Its amino acid composition is presented. [less ▲]

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See detailMolecular weight characterisation of polymers
Grandfils, Christian ULg

Conference (2010, December 07)

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See detailMolecular weight determination by visualization of stretched polycation molecules
Bocharova, Vera; Kiriy, Anton; Stamm, Manfred et al

in PMSE Preprints (2006), 95

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See detailMolecular weight effects on polystyrene fingerprint time-of-flight secondary ion mass spectroscopy (ToF-SIMS) spectra
Vanden Eynde, X.; Bertrand, P.; Jérôme, Robert ULg

in Macromolecules (1997), 30(21), 6407-6416

Monodisperse polystyrenes (PS) of different molecular weights (Mn) synthesized by living anionic polymerization with three types of butyllithium initiator (linear, n; secondary, sec; and tertiary, tert ... [more ▼]

Monodisperse polystyrenes (PS) of different molecular weights (Mn) synthesized by living anionic polymerization with three types of butyllithium initiator (linear, n; secondary, sec; and tertiary, tert) were analyzed by ToF-SIMS (time-of-flight secondary ion mass spectrometry). The influence of the molecular weight on the secondary ion intensities was studied in detail for the fingerprint part of the mass spectra (with m/z < 200). A drastic effect was observed for Mn values below 104, related to the presence of the saturated butyl end group. An extra hydrogen transfer originating from this end group during the secondary ion formation must be invoked to explain the data. Only the first neighbor monomer repeat units seem to be affected. This H exchange increases the intensity of ions containing more hydrogen or needing H transfer for their formation as the tropylium ion (C7H7+ at m/z = 91). The molecular structure of the butyl end group is found to influence greatly not only the intensity of their parent ion but also the PS characteristic ion intensities. Indeed, the tert-butyl end group is seen unable to produce the H transfer observed for the n- and sec-butyl ones. A model is proposed to take the influence of the end group on the PS SIMS fragmentation pattern into account. The parameters of this model allow the quantification of the end group interaction. [less ▲]

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See detailMolecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39
Frère, Jean-Marie ULg; Moreno, Ramon; Ghuysen, Jean-Marie ULg

in Biochemical Journal (1974), 143(1), 233-240

The exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R39 was purified to protein homogeneity and in milligram amounts. The isolated enzyme consisted of one polypeptide chain of molecular ... [more ▼]

The exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R39 was purified to protein homogeneity and in milligram amounts. The isolated enzyme consisted of one polypeptide chain of molecular weight about 53300. Its amino acid composition and several physicochemical properties were determined and compared with those of the exo-cellular dd-carboxypeptidase-transpeptidase from Streptomyces R61. [less ▲]

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See detailMolecule orientation in self-assembled monolayers determined by infrared-visible sum-frequency generation spectroscopy
Mani, A. A.; Schultz, Z. D.; Champagne, B. et al

in Applied Surface Science (2004), 237

The molecular orientation in self-assembled monolayers of thiophenol (TP) on Ag(1 1 1) and of para-nitroanilinododecane-thiol (NAT) on Au was investigated by infrared-visible sum-frequency generation ... [more ▼]

The molecular orientation in self-assembled monolayers of thiophenol (TP) on Ag(1 1 1) and of para-nitroanilinododecane-thiol (NAT) on Au was investigated by infrared-visible sum-frequency generation between 500 and 1500 cm-1. Ab initio calculations were performed to determine the interface nonlinear response. The combined experimental and theoretical results reveal that the S–C bond of TP is tilted 37° from the Ag(1 1 1) surface normal, with the aromatic ring plane perpendicular to the surface. On Au, the tilt angle of the NAT molecule stabilizes below 60° at the end of film growth. [less ▲]

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See detailMolecule-based photonically switched half and full adder
Remacle, Françoise ULg; Weinkauf, R.; Levine, R. D.

in Journal of Physical Chemistry A (2006), 110(1), 177-184

A single molecule logic gate using electronically excited states and ionization/fragmentation can take advantage of the differences in cross-sections for one and two photon absorption. Fault tolerant ... [more ▼]

A single molecule logic gate using electronically excited states and ionization/fragmentation can take advantage of the differences in cross-sections for one and two photon absorption. Fault tolerant optically pumped half adder and full adder are discussed as applications. A full adder requires two separate additions, and the logic concatenation that is required to implement this is physically achieved by an intramolecular transfer along the side chain of 2-phenylethyl-N,N-dimethylamine (PENNA). Solutions of the kinetic equations for the temporal evolution of the concentration of different states in the presence of time-varying laser fields are used to illustrate the high contrast ratios that are potentially possible for such devices. [less ▲]

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See detailMolécules de la famille des protéases aspartiques dans le placenta des ruminants: hormones ou protéines ?
Beckers, Jean-François ULg; Roberts, R. Michael; Zoli, André Pagnah et al

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1994), 149(8-11), 355-367

The placenta of ruminant contains binucleate trophoblastic cells synthesizing proteins, migrating cross the barrier and fusing with endothelial cells of the endometrium. Recently described were two ... [more ▼]

The placenta of ruminant contains binucleate trophoblastic cells synthesizing proteins, migrating cross the barrier and fusing with endothelial cells of the endometrium. Recently described were two glycoproteins from the family of aspartic proteases, apparently lacking the enzymatic activity: the pregnancy associated glycorproteins I and II (PAGI and PAGII). The first (PAGI) is largely secreted in maternal blood, this characteristic copes with the lack of proteolytic activity. The second (PAGII) is not completely characterized. However, it binds to lutropin (LH) receptors with high affinity. This binding allows to assume that PAGII is likely the same as the bovine chorionic gonadotropin identified earlier (bCG). A better characterization of these glycoproteins (PAGI and PAGII) and other members of the family (PAGIII...) will answer these questions together with the unexplained invasive process of the placenta. [less ▲]

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See detailMolécules diatomiques : étude des termes spectraux
Swings, Polydore ULg

Book published by Hermann (1933)

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See detailMolecules: their role in astronomy
Swings, Polydore ULg

in Publications of the Astronomical Society of the Pacific [=PASP] (1942), 54

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See detailMolière au théâtre, les Médecins à la Ville
Andrien, Natacha; Jaminon, Martine ULg

Learning material (2010)

Plaquette de l'exposition - Perception de la médecine du 17ème siècle à travers l'oeuvre de Molière

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See detailMollino, Carlo
Prina, Daniela ULg

in Atkinson, Harriet; Kettley, Sarah; Edwards, Clive (Eds.) et al Encyclopedia of Design (in press)

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See detailMolybdenite Re-Os dating constrains gravitational collapse of the Sveconorwegian orogen, SW Scandinavia
Bingen, Bernard; Stein, Holly J.; Bogaerts, Michel et al

in Lithos (2006), 87(3-4), 328-346

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See detailMoment capacity of beam-to-column minor-axis joints
Gomes, F.; Jaspart, Jean-Pierre ULg; Maquoi, René ULg

in Proceedings of the IABSE Colloquium on Semi-Rigid Structural Connections (1996)

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See detailMoment Method and Pixel-by-Pixel Method: Complementary Mode Identification I. Testing FG Vir-like pulsation modes
Zima, W.; Kolenberg, K.; Briquet, Maryline ULg et al

in Communications in Asteroseismology (2004), 144

We have carried out a Hare-and-Hound test to determine the reliability of the Moment Method (Briquet & Aerts 2003) and the Pixel-by-Pixel Method (Mantegazza 2000) for the identification of pulsation modes ... [more ▼]

We have carried out a Hare-and-Hound test to determine the reliability of the Moment Method (Briquet & Aerts 2003) and the Pixel-by-Pixel Method (Mantegazza 2000) for the identification of pulsation modes in Delta Scuti stars. For this purpose we calculated synthetic line profiles, exhibiting six pulsation modes of low degree and with input parameters initially unknown to us. The aim was to test and increase the quality of the mode identification by applying both methods independently and by using a combined technique. Our results show that, whereas the azimuthal order m and its sign can be fixed by both methods, the degree l is not determined unambiguously. Both identification methods show a better reliability if multiple modes are fitted simultaneously. In particular, the inclination angle is better determined. We have to emphasize that the outcome of this test is only meaningful for stars having pulsational velocities below 0.2 vsini. This is the first part of a series of articles, in which we will test these spectroscopic identification methods. [less ▲]

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