Intra-operative autologous transfusion in cancer patients.
in Acta Anaesthesiologica Belgica (2004), 55(1), 49-51Detailed reference viewed: 11 (1 ULg)
Intra-operative MRI: description of a single institution experience with emphasis n the use of T2/FLAIR sequences for resection control in low-grade glioma.
Conference (2009, May 24)Detailed reference viewed: 13 (4 ULg)
Intra-patient variability of SUV in normal tissues.
; HUSTINX, Roland ; FOIDART-WILLEMS, Jacqueline
in European Journal of Nuclear Medicine and Molecular Imaging (2003), 30(SUPPL 2), 327Detailed reference viewed: 16 (1 ULg)
Intra-specific variation in nitrate tolerance in tadpoles of the Natterjack toad.
; Oromi Farrús, Neus ; et al
in Ecotoxicology (2011), 20Detailed reference viewed: 10 (0 ULg)
INTRA-TUMORAL HETEROGENEITY AND RATIONAL SELECTION OF ANTIGENS FOR TARGETED THERAPY OF LIVER METASTASES
Turtoi, Andrei ; Blomme, Arnaud ; Delvaux, David et al
in Acta Chirurgica Belgica (2012, May), 112(3), 8953
Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer ... [more ▼]
Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer cells causing treatment resistance and relapse. Therefore, a rational strategy is needed to select targetable antigens that would overcome this intra-tumoral heterogeneity. Methods: After ethical committee approval, 48 fresh liver metastases of colorectal origin were prospectively collected from patients undergoing liver resection. Here we macroscopically divided the lesion in different zones and generated a unique quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. Particular focus was laid on accessible proteins, a protein subclass comprising cell membrane associated and extracellular proteins. Accordingly, the tissues were ex-vivo biotinylated, affinity purified and analyzed for each zone separately using nano-UPLC-MSe proteomics technique. In total over 1500 unique proteins were statistically divided into different patterns of expression. Results: We have generated a quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. The study offers insight into novel targets but also antigens against which the antibodies are already involved in clinical trials or treatment of liver metastases. Extensive clustering and validation experiments highlight novel markers that offer the potential to homogeneously cover the metastatic lesion and become better targets. Conclusions: Two such antigens, LTBP2 and TGFBI were selected for functional analysis in colorectal carcinoma cells. In vitro and in vivo experiments showed that in particular TGFBI is relevant for migration and proliferation capacity of colorectal cancer cells. The suppression of this protein led to significant inhibition of tumor growth, crystalizing it as bona fide target for the development of anti-metastases therapies. [less ▲]Detailed reference viewed: 84 (22 ULg)
Intraarterial spiral CT angiography combined with DSA in pretherapeutic assessment of cerebral aneurysms.
; ; Dondelinger, Robert et al
Conference (1996, September 21)Detailed reference viewed: 12 (0 ULg)
Intracardiac flow visualization: current status and future directions.
; ; et al
in European Heart Journal - Cardiovascular Imaging (2013)
Non-invasive cardiovascular imaging initially focused on heart structures, allowing the visualization of their motion and inferring its functional status from it. Colour-Doppler and cardiac magnetic ... [more ▼]
Non-invasive cardiovascular imaging initially focused on heart structures, allowing the visualization of their motion and inferring its functional status from it. Colour-Doppler and cardiac magnetic resonance (CMR) have allowed a visual approach to intracardiac flow behaviour, as well as measuring its velocity at single selected spots. Recently, the application of new technologies to medical use and, particularly, to cardiology has allowed, through different algorithms in CMR and applications of ultrasound-related techniques, the description and analysis of flow behaviour in all points and directions of the selected region, creating the opportunity to incorporate new data reflecting cardiac performance to cardiovascular imaging. The following review provides an overview of the currently available imaging techniques that enable flow visualization, as well as its present and future applications based on the available literature and on-going works. [less ▲]Detailed reference viewed: 38 (0 ULg)
Intracellular and extracellular vaccinia virions enter cells by different mechanisms
Vanderplasschen, Alain ; ;
in Journal of General Virology (The) (1998), 79(Pt 4), 877-887Detailed reference viewed: 16 (0 ULg)
Intracellular cation concentrations in essential hypertension and chronic renal failure
Krzesinski, Jean-Marie ; ; Rorive, Georges
in Clinical & Experimental Hypertension (1993), 15(3), 461-478
The aim of this study was to test basal and after treatment erythrocyte sodium and calcium concentrations, and calcium-ATPase activity and platelet cytosolic free calcium and pH in 20 normotensive ... [more ▼]
The aim of this study was to test basal and after treatment erythrocyte sodium and calcium concentrations, and calcium-ATPase activity and platelet cytosolic free calcium and pH in 20 normotensive controls, 20 hemodialysis-dependent chronic renal failure patients and in 18 essential hypertensives. Prior to treatment, essential hypertensive and uremic patients presented similar higher platelet calcium concentrations and lower pH than the normotensive control group. The erythrocyte sodium, calcium, and magnesium concentrations were only significantly elevated in chronic renal failure, with a significant decrease in the calcium-ATPase activity in the latter population. Hemodialysis partially reversed these intracellular ionic abnormalities with normalization of platelet pH. Significant correlations have been noted between weight loss and decreases in platelet calcium concentration (r = 0.60, p < 0.01) or in erythrocyte sodium (r = 0.50, p < 0.05). The systolic blood pressure decrease was only correlated to the increase in calcium-ATPase activity (r = 0.57, p < 0.05). Antihypertensive treatment (captopril and nifedipine) only tended to normalize the intracellular calcium concentration with correlation between the decrease of the latter and blood pressure decrease (r = 0.64 for the systolic blood pressure and 0.68 for the diastolic blood pressure, p < 0.01). Thus, in essential hypertension and in uremia, some cellular ionic abnormalities exist in platelets in baseline condition. Moreover, in uremia, erythrocyte presents abnormal ionic pattern. Some, but not all of these abnormalities could be corrected by treatment affecting blood pressure (cellular calcium) in essential hypertension or by hemodialysis (cellular sodium, calcium, and pH). In the latter treatment, the changes are linked to extracellular fluid modification. In essential hypertension, the intracellular calcium reduction was linked to blood pressure decrease. [less ▲]Detailed reference viewed: 12 (0 ULg)
Intracellular concentration of cryoprotectant during vitrification and slow-freezing cryopreservation procedures
Vanderzwalmen, Pierre ; Ectors, Fabien ; et al
in Tucker, Mickael; Lieberman, Juergen (Eds.) Vitrification in assisted reproduction: second edition (2015)Detailed reference viewed: 12 (1 ULg)
Intracellular damages induced by singlet oxygen are signals for HIV-1 reactivation
Piette, Jacques ; Legrand-Poels, Sylvie
in Journal of Free Radicals in Biology & Medicine (1993)Detailed reference viewed: 6 (1 ULg)
Intracellular distribution of the ORF4 gene product of varicella-zoster virus is influenced by the IE62 protein
; ; et al
in Journal of General Virology (1996), 77(Part 7), 1505-1513
Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for genes of heterologous viruses, The major regulatory immediate ... [more ▼]
Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for genes of heterologous viruses, The major regulatory immediate-early protein of VZV (IE62) is a transactivator of VZV gene expression, In transfection assays, IE4 has been shown to enhance activation induced by IE62, To investigate the functional interactions underlying this observation, indirect immunofluorescence studies were undertaken to determine whether IE62 could influence IE4 intracellular localization in transfected cells, In single transfections, IE4 was predominantly found in cytoplasm, In cotransfection with IE62, the IE4 localization pattern was altered, with nuclear staining predominating over cytoplasmic staining, This effect was specific to the IE62 protein since the gene products of ORF63 and ORF61, which are also regulatory proteins, did not influence IE4 distribution, The use of IE62 mutants indicated that IE62 influence is independent of its transactivation function and that the integrity of regions 3 and 4 is required, IE62 remained nuclear whether IE4 was present or not, These observations underline differences in the regulation of gene expression between VZV proteins and their herpes simplex virus type 1 homologues, In infected cells, IE4 was only sometimes found to colocalize with IE62 in nuclei, This observation suggests that when all VZV proteins are present, complex interactions probably occur which could diminish the influence of IE62. [less ▲]Detailed reference viewed: 31 (2 ULg)
Intracellular eukaryotic pathogens in brown macroalgae in the Eastern Mediterranean, including LSU rRNA data for the oomycete Eurychasma dicksonii .
; ; et al
in Diseases of aquatic organisms (2013), 104(1), 1--11
For the Mediterranean Sea, and indeed most of the world's oceans, the biodiversity and biogeography of eukaryotic pathogens infecting marine macroalgae remains poorly known, yet their ecological impact is ... [more ▼]
For the Mediterranean Sea, and indeed most of the world's oceans, the biodiversity and biogeography of eukaryotic pathogens infecting marine macroalgae remains poorly known, yet their ecological impact is probably significant. Based on 2 sampling campaigns on the Greek island of Lesvos in 2009 and 1 in northern Greece in 2012, this study provides first records of 3 intracellular eukaryotic pathogens infecting filamentous brown algae at these locations: Eurychasma dicksonii, Anisolpidium sphacellarum, and A. ectocarpii. Field and microscopic observations of the 3 pathogens are complemented by the first E. dicksonii large subunit ribosomal RNA (LSU rRNA) gene sequence analyses of isolates from Lesvos and other parts of the world. The latter highlights the monophyly of E. dicksonii worldwide and confirms the basal position of this pathogen within the oomycete lineage (Peronosporomycotina). The results of this study strongly support the notion that the geographic distribution of the relatively few eukaryotic seaweed pathogens is probably much larger than previously thought and that many of the world's marine bioregions remain seriously undersampled and understudied in this respect. [less ▲]Detailed reference viewed: 3 (0 ULg)
The intracellular fate of polycarbonate Polyplexes modulates the efficacy of siRNA
Frère, Antoine ; ; Peixoto, Paul et al
Poster (2013, December)Detailed reference viewed: 23 (5 ULg)
Intracellular free iron content of rat liver tissue after cold ischemia
Pincemail, Joël ; ; Detry, Olivier et al
in Transplantation Proceedings (2002), 34(3), 759-761Detailed reference viewed: 33 (5 ULg)
The intracellular pathways of cuticular chitin and (glyco)protein synthesis in the integument of moulting crabs, Carcinus maenas, as followed by the use of gold-conjugated lectin probes
Compère, Philippe ; ; Goffinet, Gerhard
in Schryvers, Dominique; Timmermans, Jean-Pierre; Verbelen, Jean-Pierre (Eds.) et al Proceedings of the 13rd European Microscopy Congress, vol. III Life Sciences (2004)Detailed reference viewed: 31 (0 ULg)
Intracellular pH-dependent efflux of the fluorescent probe pyranine in the yeast Yarrowia lipolytica
Aguedo, Mario ; ;
in FEMS Microbiology Letters (2001), 200(2), 185-189
8-Hydroxypyrene-1,3,6-trisulfonic acid (pyranine) can be used as a vital intracellular pH (pHi) indicator. In the yeast Yarrowia lipolytica, a partial efflux of the probe was detected by using the pH ... [more ▼]
8-Hydroxypyrene-1,3,6-trisulfonic acid (pyranine) can be used as a vital intracellular pH (pHi) indicator. In the yeast Yarrowia lipolytica, a partial efflux of the probe was detected by using the pH-independent wavelength of 415 nm. A simplified correction of the fluorescent signals was applied, enabling to show for this species a good near-neutral pHi maintenance capacity in a pH 3.9 medium. Octanoic acid, which is known to have toxic effects on yeast, decreased the pHi and increased the 260-nm-absorbing compounds leakage. However, this acid inhibited the fluorescent probe efflux linearly with its concentration suggesting a pHi-dependent efflux of pyranine from cells. © 2001 Federation of European Microbiological Societies. [less ▲]Detailed reference viewed: 12 (0 ULg)
The intracellular tyrosine residues of the ATP-gated P2X(1) ion channel are essential for its function.
Oury, Cécile ; ; et al
in FEBS Letters (2002)
In this study, we mutated the four highly conserved intracellular tyrosine residues of the P2X(1) ion channel were mutated into phenylalanine. Simultaneous electrophysiological and calcium measurements in ... [more ▼]
In this study, we mutated the four highly conserved intracellular tyrosine residues of the P2X(1) ion channel were mutated into phenylalanine. Simultaneous electrophysiological and calcium measurements in transfected human embryonic kidney (HEK 293) cells indicated that Y362F and Y370F mutants were non-functional, despite their proper plasma membrane expression. The Y16F and Y363F mutants retained 2.2% and 26% of the wild-type P2X(1) activity, respectively. However, no tyrosine phosphorylation was detected on Western blots of P2X(1) immunoprecipitates derived either from HEK 293 cell lysates or from human platelets, expressing P2X(1) endogenously. Thus, Y16, Y362, Y363 and Y370 are required for the appropriate three-dimensional structure and function of the intracellular P2X(1) domains. [less ▲]Detailed reference viewed: 24 (4 ULg)
Intracellular visualization of BrdU-labeled plasmid DNA/cationic liposome complexes.
; Thiry, Marc ; et al
in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1999), 47(9), 1159-66
Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA ... [more ▼]
Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA complexed with a cationic lipid, Vectamidine (3-tetradecylamino-N-tert-butyl-N'-tetradecylpropionamidine) in BHK21 cells. To facilitate its detection inside the cells, bromodeoxyuridine (BrdU) was incorporated into plasmid DNA under conditions that minimize plasmid alteration. BrdU was localized in cells incubated with Vectamidine/BrdU-labeled plasmid DNA complexes by immunogold labeling and electron microscopy (EM). Labeling was predominantly associated with aggregated liposome structures at the surface of and inside the cells. EM observations of cells transfected with Vectamidine/DNA complexes showed that the liposome/DNA aggregates accumulate in large vesicles in the cell cytosol. On the other hand, using rhodamine-labeled Vectamidine and revealing BrdU with FITC-conjugated antibodies permitted simultaneous detection in the cells of both components of the complexes with confocal laser scanning microscopy. The DNA and lipids co-localized at the surface of and inside the cells, indicating that the complex is internalized as a whole. Our results show that the BrdU-labeled plasmid DNA detection system can be a useful tool to visualize exogenous DNA entry into cells by a combination of electron and confocal microscopy. [less ▲]Detailed reference viewed: 26 (6 ULg)