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See detailMorphological and allozyme variation in a collection of Cucumeropsis mannii Naudin (Cucurbitaceae) from Côte d'Ivoire
Koffi, Kouamé K.; Gbotto, Ahou A.; Malice, Marie ULg et al

in Biochemical Systematics & Ecology (2008), (36), 777-789

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See detailMorphological and allozyme variation in a collection of Lagenaria siceraria (Molina) Standl. from Côte d'Ivoire
Koffi, K. K.; Baudoin, Jean-Pierre ULg; Malice, Marie et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13(2), 257-281

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See detailMorphological and ecological complexity in early eukaryotic ecosystems
Javaux, Emmanuelle ULg; Knoll, A. H.; Walter, M. R.

in Nature (2001), 412(6842), 66-69

Molecular phylogeny and biogeochemistry indicate that eukaryotes differentiated early in Earth history. Sequence comparisons of small-subunit ribosomal RNA genes suggest a deep evolutionary divergence of ... [more ▼]

Molecular phylogeny and biogeochemistry indicate that eukaryotes differentiated early in Earth history. Sequence comparisons of small-subunit ribosomal RNA genes suggest a deep evolutionary divergence of Eukarya and Archaea(1); C-27-C-29 steranes (derived from sterols synthesized by eukaryotes) and strong depletion of C-13 (a biogeochemical signature of methanogenic Archaea) in 2,700 Myr old kerogens independently place a minimum age on this split(2,3). Steranes, large spheroidal microfossils, and rare macrofossils of possible eukaryotic origin occur in Palaeoproterozoic rocks(4-6). Until now, however, evidence for morphological and taxonomic diversification within the domain has generally been restricted to very late Mesoproterozoic and Neoproterozoic successions(7). Here we show that the cytoskeletal and ecological prerequisites for eukaryotic diversification were already established in eukaryotic microorganisms fossilized nearly 1,500 Myr ago in shales of the early Mesoproterozoic Roper Group in northern Australia. [less ▲]

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See detailMorphological and functional characterization of Carnobacterium maltaromaticum isolated from vacuum-packed beef with long shelf life
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Ndedi Ekolo, François et al

Poster (2014, August)

The aim of this study was to perform a morphological and functional characterization of a Carnobacterium maltaromaticum strain with a potential bioprotective effect isolated from vacuum packaged long ... [more ▼]

The aim of this study was to perform a morphological and functional characterization of a Carnobacterium maltaromaticum strain with a potential bioprotective effect isolated from vacuum packaged long shelf life beef. The morphological, biochemical and enzymatic profiles, the influence of different temperatures and atmospheres, and the microbial stability of fresh beef inoculated with the C. maltaromaticum strain were evaluated. The isolated C. maltaromaticum strain presented similar morphological, biochemical and enzymatic profiles as those of two reference strains (LMG 11393 and LMG 22902). The growth of C. maltaromaticum was slower in an atmosphere containing O2 and CO2. Vacuum packing is therefore suitable for this bacterium. An antimicrobial effect against Enterobacteriaceae was highlighted on inoculated fresh meat stored under N2. The functional characterization of this isolate will be further pursued by a genotypic characterization to better understand its potential bioprotective effect. [less ▲]

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See detailMORPHOLOGICAL AND GENETIC CRITERIA IN THE TAXONOMY OF CYANOPHYTA CYANOBACTERIA
Wilmotte, Annick ULg; Golubic, Stjepko

in Archiv für Hydrobiologie (1991), 92

Molecular techniques have introduced new approaches to the phylogeny and taxonomy of Cyanophyta/Cyanobacteria. One method, the sequencing of the 16S ribosomal RNA, has been applied to 29 cyanophyte ... [more ▼]

Molecular techniques have introduced new approaches to the phylogeny and taxonomy of Cyanophyta/Cyanobacteria. One method, the sequencing of the 16S ribosomal RNA, has been applied to 29 cyanophyte strains and produced interesting results (TOMIOKA & SUGIURA 1983, GIOVANNONI et al. 1988). Integration of this new, taxonomically useful information with the results of the classical morphological analyses traditionally used in the taxonomy of cyanophytes is the subject of this paper. Advantages and limitations of different approaches are discussed, and conflicts as well as consensus between morphological properties of the strains under study, and their position in the phylogenetic tree of GIOVANNONI et al. (1988) are reviewed. [less ▲]

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See detailMorphological and genetic divergences in a coral reef damselfish, Pomacentrus coelestis
Frederich, Bruno ULg; Liu, S.-Y. Vanson; Dai, C.-F.

in Evolutionary Biology (2012), 39(3), 359-370

Population differentiation is one of the main topics in evolutionary biology. Except the exploration of color variation, few studies focused on morphological divergences among populations of coral reef ... [more ▼]

Population differentiation is one of the main topics in evolutionary biology. Except the exploration of color variation, few studies focused on morphological divergences among populations of coral reef fishes. In this work, we studied morphological and genetic differentiation among populations of the damselfish, Pomacentrus coelestis, in the northwestern Pacific Ocean. The shapes of the mandible and the premaxilla were explored using geometric morphometric methods and the genetic structure was investigated using microsattelites. Various tests revealed significant shape variation among most P. coelestis populations for both skeletal units. Morphological variation of the mandible accompanies a genetic break between populations of mainland Japan and Okinawa-Taiwan. However, Mantel and Procrustes tests revealed no congruence between morphological and genetic structures. We illustrate that phenotypic plasticity and adaptive divergence are potential evolutionary mechanisms underlying shape difference among P. coelestis populations. An ecomorphological approach suggests that various diet could be related to shape variation of oral jaws. [less ▲]

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See detailMorphological and histological studies of sheep’s brain
Salouci, Moustafa ULg; Engelen, Virginie; Jacqmot, Olivier ULg et al

Poster (2011, December)

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the ... [more ▼]

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and cerebellum cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus. [less ▲]

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See detailMorphological and histological studies of sheep’s brain
Salouci, Moustafa ULg; Engelen, Virginie; Jacqmot, Olivier ULg et al

Poster (2010)

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the ... [more ▼]

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and cerebellum cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus. [less ▲]

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See detailMorphological and hormonal characterisation of strawberry vitroplants raised through axillary or stipular adventitious shooting
Jemmali, Ahmed; Elloumi, Nedra; Kevers, Claire ULg et al

in Plant Growth Regulation (2002), 38(3), 273-278

Adventitious stipular bud formation occurred in vitro in many strawberry cultivars during the proliferation phase on medium containing Knop macronutrients, MS micronutrients, vitamins, aminoacids, 2.22 ... [more ▼]

Adventitious stipular bud formation occurred in vitro in many strawberry cultivars during the proliferation phase on medium containing Knop macronutrients, MS micronutrients, vitamins, aminoacids, 2.22 muM BAP, 2.46 muM IBA and 0.29 muM GA(3). As described previously for cultivar Gorella, cultivar Elsanta also showed adventitious stipular buds developing on the abaxial median zone between the stipule tips. To compare the shoots produced from both types of buds, clonal propagation was initiated from stipular buds and from axillary buds on the above mentioned medium. Stipular buds were separated from the meristem-tip initiated plantlet and cultivated in the presence of a lower BAP concentration (1.33 muM) to prevent further stipular bud formation. During proliferation cycles, stipular originated propagules were very easily distinguished by their specific leaf phenotype and light green colour in comparison to plantlets cloned for an axillary bud. Their multiplication rate and cytokinin content were also higher than for axillary buds. No significant difference was observed in auxin content. [less ▲]

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See detailMorphological and mechanical characterisation of bone scaffolds
Kerckhofs, Greet ULg; Wevers, Martine; Schrooten, Jan

Poster (2005)

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See detailMorphological and mechanical characterization of porous structures with micro-CT
Kerckhofs, Greet ULg; Wevers, Martine; Schrooten, Jan

in Abstract book SkyScan User Meeting 2005 (2005)

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See detailMorphological and mechanical characterization of Ti6Al4V scaffolds produced with selective laser melting
Van Bael, Simon; Kerckhofs, Greet ULg; Moesen, Maarten et al

in Proc. ECCOMAS - International Conference on Tissue Engineering 2009. . 2009, Bártolo, P. (Eds.) (2009)

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See detailMorphological and Mechanical Quantification of Porous Structures by Means of Micro-CT (Morfologische en mechanische kwantificatie van poreuze structuren met behulp van micro-CT)
Kerckhofs, Greet ULg

Doctoral thesis (2009)

Suitable characterization techniques for porous structures are required to (i) understand and to be able to simulate, via finite elements (FE), the structure-properties relationships and (ii) understand ... [more ▼]

Suitable characterization techniques for porous structures are required to (i) understand and to be able to simulate, via finite elements (FE), the structure-properties relationships and (ii) understand the relationship between the morphology and mechanical behaviour on one hand, and the failure mechanisms on the other hand. X-ray microfocus computed tomography (micro-CT) offers a solution as it provides a means to acquire a complete 3D set of images of the structure visualizing the internal architecture at the microscopic level in a non-destructive way. Additionally, the micro-CT images enable subsequent image analysis, resulting in an extensive 3D quantitative description of the morphology that cannot be obtained by other methods. But, one has to be aware of the fact that micro-CT images are inherently subjected to artefacts and that the image quality and accuracy depend on multiple factors. For example, the acquisition settings (target material, tube voltage and filter material) influence the X-ray spectra and hence also the image quality. The spatial resolution strongly influences the accuracy of the micro-CT images and as a result also the morphological analysis. Closely related to the latter, the material architecture has a significant influence on the micro-CT image accuracy, which is also affected by the material type. In a first part of this study, systematic, fast and user-independent protocols have been developed both for acquisition parameter optimization and for image accuracy validation, and the influence of the different factors mentioned above on the image accuracy has been investigated. As a result, when applying these protocols, the micro-CT user should know, for different material types and architectures, what the capabilities and limitations of micro-CT are for morphological assessment of porous structures. In a second part, this knowledge has been applied and the use of micro-CT has been expanded to the mechanical characterization of porous structures by combining micro-CT imaging and 3D image analysis with in-situ mechanical loading, FE analysis and local strain mapping as this combination allows to (i) provide in-situ and experimentally the mechanical properties, (ii) link the mechanical properties to the morphology, (iii) investigate the morphological changes under compressive loading, (iv) feed and validate a FE model which can be applied for the prediction of the mechanical properties that cannot readily be determined experimentally and (v) predict the failure modes by using experimental local strain mapping. [less ▲]

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See detailMorphological and Molecular Characterization of Lymnaeid Snails and Their Potential Role in Transmission of Fasciola spp. in Vietnam
Bui Thi, Dung ULg; Pham Ngoc, Doanh; Dang Tat, The et al

in Korean Journal of Parasitology (2013), 51(6), 657-662

Freshwater snails of the family Lymnaeidae play an important role in the transmission of fascioliasis worldwide. In Vietnam, 2 common lymnaeid species, Lymnaea swinhoei and Lymnaea viridis, can be ... [more ▼]

Freshwater snails of the family Lymnaeidae play an important role in the transmission of fascioliasis worldwide. In Vietnam, 2 common lymnaeid species, Lymnaea swinhoei and Lymnaea viridis, can be recognized on the basis of morphology, and a third species, Lymnaea sp., is known to exist. Recent studies have raised controversy about their role in transmission of Fasciola spp. because of confusion in identification of the snail hosts. The aim of this study is, therefore, to clarify the identities of lymnaeid snails in Vietnam by a combination of morphological and molecular approaches. The molecular analyses using the second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA clearly showed that lymnaeids in Vietnam include 3 species, Austropeplea viridis (morphologically identified as L. viridis), Radix auricularia (morphologically identified as L. swinhoei) and Radix rubiginosa (morphologically identified as Lymnaea sp.). R. rubiginosa is a new record for Vietnam. Among them, only A. viridis was found to be infected with Fasciola spp. These results provide a new insight into lymnaeid snails in Vietnam. Identification of lymnaeid snails in Vietnam and their role in the liver fluke transmission should be further investigated. [less ▲]

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See detailMorphological and molecular characterization of planktonic cyanobacteria from Belgium and Luxembourg
Willame, R.; Boutte, C.; Grubisic, Stana ULg et al

in Journal of Phycology (2006), 42(6), 1312-1332

For the first time in Belgium and Luxembourg, the diversity and taxonomy of 95 cyanobacterial strains isolated from freshwater blooms were assessed by the comparison of phenotypes and partial 16S rRNA ... [more ▼]

For the first time in Belgium and Luxembourg, the diversity and taxonomy of 95 cyanobacterial strains isolated from freshwater blooms were assessed by the comparison of phenotypes and partial 16S rRNA gene sequences. The results showed the high diversity of nanoplanktonic, picoplanktonic, and benthic-periphytic cyanobacteria accompanying the main bloom-forming taxa. Indeed, besides 15 morphotypes of bloom-forming taxa, seven non-bloom-forming planktonic morphotypes and 11 morphotypes from benthic-periphytic taxa were isolated in culture from the plankton samples of 35 water bodies. The bloom-forming strains belonged to the genera Microcystis, Woronichinia, Planktothrix, Anabaena, and Aphanizomenon, whereas the other strains isolated from the same samples were assigned to the nanoplanktonic Aphanocapsa, Aphanothece, Snowella, and Pseudanabaena; to the picoplanktonic Cyanobium; and to the benthic periphytic Geitlerinema, Komvophoron, Leptolyngbya, Lyngbya, Phormidium, Calothrix, Nostoc, and Trichormus. The results supported both the polyphyletism of genera such as Aphanocapsa, Aphanothece, Leptolyngbya, Geitlerinema, Anabaena, and Aphanizomenon as well as the validity of genera such as Microcystis, Planktothrix, and Pseudanabaena with gas vesicles and cells constricted at the cross wall. The results obtained showed the close relationship between Snowella and Woronichinia for which very few sequences exist. The first sequence of Komvophoron appeared poorly related to other available cyanobacterial sequences. Although in a few cases a good agreement existed between phenotypic and genotypic features, there was generally a discrepancy. Strains with identical morphotypes show small differences in the 16S rRNA sequences, which might be related to the different chemical properties of their habitats. The results showed the importance of the polyphasic approach in order to improve the taxonomy of cyanobacteria. [less ▲]

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See detailMorphological and molecular diversity within Algerian cowpea (Vigna unguiculata (L.) Walp.) landraces
Ghalmi, Naima; Malice, Marie ULg; jacquemin, Jean-Marie et al

in Genetic Resources and Crop Evolution (2010), (57), 371-386

Detailed reference viewed: 107 (12 ULg)