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See detailAntimicrobial Effects of Bifidobacteria from Human and Animal Origin
Delcenserie, Véronique ULg

Scientific conference (2015)

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See detailAntimicrobial potential of probiotic or potentially probiotic lactic acid bacteria, the first results of the international European research project PROPATH of the PROEUHEALTH cluster
De Vuyst, L.; Lefteris, M.; Lazlo, A. et al

in Microbial Ecology in Health & Disease (2004), 16

The EU-funded PROPATH project addresses the important health issue of prevention of gastrointestinal disorders through probiotics and prebiotics. Seven European laboratories are co-operating in this ... [more ▼]

The EU-funded PROPATH project addresses the important health issue of prevention of gastrointestinal disorders through probiotics and prebiotics. Seven European laboratories are co-operating in this project, which aims to isolate and characterize the relevant antimicrobial agents to combat Gram-negative bacteria including Helicobacter pylori and Salmonella enterica serovar Typhimurium. In this paper, the first results on the screening for probiotic or potentially probiotic lactobacilli that exhibit antimicrobial activity towards these Gram-negative pathogenic bacteria are presented. Spot-on-lawn assays, well-diffusion assays and time-kill studies were performed among the lactic acid bacteria strains that were either collected from fermented foods and faeces (breast-fed babies, infants and animals) or isolated from commercial products to investigate whether any of the collected strains were inhibiting growth of or were killing certain indicator bacteria. Strains inhibiting the gastrointestinal pathogens mentioned above were found. Evidence has been obtained that compounds different from organic acids are produced. [less ▲]

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See detailAntimicrobial properties of Pseudomonas strains producing the antibiotic mupirocin
Matthijs, S.; Vander Wauven, C.; Cornu, B. et al

in Research in Microbiology (2014), 165

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See detailAntimicrobial resistance in Salmonella strains isolated during the zoonosis monitoring in slaughterhouses, meat plants and retail shops in 2001
Dierick, K.; Wybo, I.; Jouret, M. et al

Poster (2002, June 20)

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See detailAntimicrobial Resistance in the food chain: a review
Verraes, Claire; Van Boxtael, Sigrid; Van Meervenne, Eva et al

in International Journal of Environmental Research and Public Health (2013), 10

Antimicrobial resistant zoonotic pathogens present on food constitute a direct risk to public health. Antimicrobial resistance genes in commensal or pathogenic strains form an indirect risk to public ... [more ▼]

Antimicrobial resistant zoonotic pathogens present on food constitute a direct risk to public health. Antimicrobial resistance genes in commensal or pathogenic strains form an indirect risk to public health, as they increase the gene pool from which pathogenic bacteria can pick up resistance traits. Food can be contaminated with antimicrobial resistant bacteria and/or antimicrobial resistance genes in several ways. A first way is the presence of antibiotic resistant bacteria on food selected by the use of antibiotics during agricultural production. A second route is the possible presence of resistance genes in bacteria that are intentionally added during the processing of food (starter cultures, probiotics, bioconserving microorganisms and bacteriophages). A last way is through cross-contamination with antimicrobial resistant bacteria during food processing. Raw food products can be consumed without having undergone prior processing or preservation and therefore hold a substantial risk for transfer of antimicrobial resistance to humans, as the eventually present resistant bacteria are not killed. As a consequence, transfer of antimicrobial resistance genes between bacteria after ingestion by humans may occur. Under minimal processing or preservation treatment conditions, sublethally damaged or stressed cells can be maintained in the food, inducing antimicrobial resistance build-up and enhancing the risk of resistance transfer. Food processes that kill bacteria in food products, decrease the risk of transmission of antimicrobial resistance. [less ▲]

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See detailAntimicrobial susceptibilities of Campylobacter strains isolated from food animals
Van Looveren, M.; Daube, Georges ULg; De Zutter, L. et al

Poster (1999, September)

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See detailAntimicrobial Susceptibilities of recent clinical isolates of group B streptococci agalactiae from Belgium
MELIN, Pierrette ULg; Maquet, Julie; Rodriguez Cuns, Grisel et al

in American Society of Microbiology (Ed.) Program and Abstracts of the 43rd Intersciences Conference on Antimicrobial Agents and Chemotherapy (2003, September)

Background: : GBS cause severe infections in neonates, pregnant women and other adults. Empiric therapy is usually started before susceptibility results are available. Early neonatal diseases can be ... [more ▼]

Background: : GBS cause severe infections in neonates, pregnant women and other adults. Empiric therapy is usually started before susceptibility results are available. Early neonatal diseases can be prevented with intrapartum antibiotic prophylaxis based on accurate susceptibility surveillance data. A previous Belgian study showed an increase of 3 to 10 % R to erythromycin (EM) through the 1990s. Methods: 187 GBS isolates consecutively received at the reference laboratory between 2001 to March 2003 were from 73 neonates (52 early-onset and 21 late-onset diseases), 52 adults and 62 from pregnant women’s vagina. MICs of penicillin (PG), EM, clindamycin (CM) and gentamicin (GM) were determined with Etest. PG MBCs were also determined by inactivating the drug in MIC plates using betalactamase. EM resistant (R) isolates were tested by the CM + EM double disk to determine macrolide R phenotypes. Results: All strains were susceptible (S) to PG and no tolerance was observed with MBCs falling within 2 dilutions of MICs. 19.2% of isolates were R to EM, with significantly more R isolates from adults (30.8%; p <0.01) and serotype V (46.8%; p <0,001). 80% had the MLSB phenotype (R to EM and CM), 16 were constitutive and 12 inducible. The M phenotype (R to EM and S to CM) was seen in 7 (20%) of isolates. Less than 10% of isolates were inhibited by GM MIC of <=64 mg/L, 83.6% by 128-256 mg/L and 2.9% by >/=512 mg/L. Non typable strains were more R to GM (p <0.01). Conclusions: 1) PG remained active against all isolates and no tolerance was seen. 2) Prevalence of R to macrolides had increased since 1999, particularly in adult isolates and serotype V. 3) Intermediate to high level R to GM was seen and potential synergy of PG + GM should be investigated. 4) R surveillance is mandatory to guide prophylaxis and treatment of serious GBS infections. [less ▲]

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See detailAntimicrobial susceptibilities of Salmonella strains isolated from food animals
Van Looveren, M.; Daube, Georges ULg; De Zutter, L. et al

Poster (1999, September)

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See detailAntimicrobial Susceptibility of Streptococcus agalactiae Isolated from Patients in Belgium Through 1989-1991 and 1996-1999
MELIN, Pierrette ULg; Rodriguez Cuns, Grisel; Vincento Fernandez, Walter et al

in Martin, Diana R.; Tagg, John R. (Eds.) Streptococci and Streptococcal Diseases Entering the New Millenium - Proceeding of the XIV LISSSD (2000, November)

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See detailAntimicrobial Susceptibility of Streptococcus agalactiae Isolated from Patients in Belgium through 1989-1991 and 1996-1999
Melin, Pierrette ULg; Rodriguez Cuns, Grisel; Vicentino Fernandez, Walter et al

in Martin, Diana R; Tagg, John R (Eds.) Streptococci and Streptococcal Diseases - Entering the New Millenium", Proceedings of the XIV Lancefield International Symposium on Streptococci and Streptococcal Diseases (2000)

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See detailAntimicrobial Susceptibility of Streptococcus agalactiae isolated from patients in Belgium through 1989-1991 and 1996-1999
MELIN, Pierrette ULg; Rodriguez Cuns, Grisel; Vincento Fernandez, Walter et al

Poster (1999, October)

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See detailAntimicrobial susceptilities of Campylobacter strains isolated from food animals in Belgium.
Van Looveren, M.; Daube, Georges ULg; De Zutter, L. et al

in Journal of Antimicrobial Chemotherapy (2001), 48

Afin d’assurer une transparence tout le long de la chaîne de transformation de la viande, il faut disposer d’un système de traçabilité performant. La traçabilité administative montre des limites que ... [more ▼]

Afin d’assurer une transparence tout le long de la chaîne de transformation de la viande, il faut disposer d’un système de traçabilité performant. La traçabilité administative montre des limites que l’utilisation de marqueurs génétiques pourrait surmonter. Le génome de chaque individu possède des différences de séquences, à la base du polymorphisme génétique, dont les marqueurs génétiques sont les témoins. Parmi ceux-ci, deux classes semblent s’imposer en matière de traçabilité : les microsatellites et les polymorphismes simple nucléotide. Les microsatellites sont caractérisés par un degré de polymorphisme important avec de nombreux allèles pour un même locus. De plus, leur détection se fait directement par amplification itérative. Techniquement, le problème se pose lors de la détection simultanée de plusieurs microsatellites où le patron obtenu est difficile à interpréter. Les polymorphismes simple nucléotide sont répresentés par des mutations ponctuelles dans la séquence nucléotidique. Ils sont fréquents, stables, répartis de façon aléatoire et généralement bialléliques. La détection se fait soit par hybridation sur des biopuces, soit par spectrométrie de masse ou par d’autres techniques facilement automatisables. Cette automatisation est nécessaire pour pouvoir tester un grand nombre de polymorphismes simultanément sur un grand nombre d’échantillons, permettant ainsi de diminuer les coûts. L’avantage majeur des polymophismes simple nucléotide est le signal binaire obtenu, son désavantage est le caractère population- spécifique de ce type de marqueur. [less ▲]

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See detailAntimicrobial susceptilities of Salmonella strains isolated from food animals.
Van Looveren, M.; Daube, Georges ULg; De Zutter, L. et al

in Antimicrobial Agents and Chemotherapy (2001), 48

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See detailAntimicrobial, antitumor and brine shrimp lethality assay of Ranunculus arvensis L. extracts
Muhammad Zeeshan Bhatti; Ali, Amjad ULg; Asma Saeed et al

in Pakistan Journal of Pharmaceutical Sciences (2015)

To investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of Methanol Extract (ME), Water Extract (WE), Acetone Extract (AE), Chloroform Extract (CE ... [more ▼]

To investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of Methanol Extract (ME), Water Extract (WE), Acetone Extract (AE), Chloroform Extract (CE), Methanol-Water Extract (MWE), Methanol-Acetone Extract (MAE), Methanol-Chloroform Extract (MCE) of Ranunculus arvensis (L.). Antitumor activity was evaluated with Agrobacterium tumefaciens (At10) induced potato disc assay. Cytotoxicity was evaluated with brine shrimp lethality assay. Antibacterial activity was evaluated with six bacterial strains including Escherichia coli, Enterobacter aerogenes, Bordetella bronchiseptica, Klebsiella pneumoniae, Micrococcus luteus and Streptococcus anginosus and antifungal screening was done against five fungal strains including Aspergillus niger, A. flavus, A. fumigates, Fusarium solani and Mucor species by using disc diffusion method. Best antitumor activity was obtained with ME and WE, having highest IC50 values 20.27±1.62 and 93.01±1.33μg/disc. Brine shrimp lethality assay showed LC50 values of AE, MAE and ME were obtained as 384.66±9.42μg/ml, 724.11±8.01μg/ml and 978.7±8.01μg/ml respectively. WE of R. arvensis revealed weak antimicrobial result against the tested microorganisms. On the other hand, the antifungal activity of the plant extracts was found to be insignificant. These findings demonstrate that extracts of R. arvensis possesses significant antitumor activity. Further extensive study is necessary to assess the therapeutic potential of the plant [less ▲]

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See detailAntimitotic activity of strychnopentamine, a bisindolic alkaloid
Tits, Monique ULg; Desaive, Claude ULg; Marnette, J. M. et al

in Journal of Ethnopharmacology (1984), 12

Strychnopentamine has been tested for its cytotoxicity and antitumor activities and compared with two other bisindolic alkaloids that possess an usambarane skeleton. The presence of a N-methylpyrrolidine ... [more ▼]

Strychnopentamine has been tested for its cytotoxicity and antitumor activities and compared with two other bisindolic alkaloids that possess an usambarane skeleton. The presence of a N-methylpyrrolidine group increases the antimitotic activity of this type of alkaloids. [less ▲]

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See detailAntimitotic and cytotoxic Activities of Guattegaumerine, a Bisbenzylisoquinoline Alkaloid
Quetin-Leclercq, Joëlle; De Pauw-Gillet, Marie-Claire ULg; Bassleer, Roger et al

in Planta Medica (1987), 53

Guattegaumerine was isolated from the bark of Guatteria gaumeri ( cf Dehaussy, Tits and Angenot, Planta Med. (1983) 49, 25-27) , a medicinal plant called Yumel in Yucatan (South Mexico).Guattegaumerine ... [more ▼]

Guattegaumerine was isolated from the bark of Guatteria gaumeri ( cf Dehaussy, Tits and Angenot, Planta Med. (1983) 49, 25-27) , a medicinal plant called Yumel in Yucatan (South Mexico).Guattegaumerine exerts a good activity at 5 micrograms/ml on B16 melanoma which is a relatively resistant tumor. It is less active against 2002 cells that are normal human cells. [less ▲]

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See detailAntimony vibrations in skutterudites probed by Sb-121 nuclear inelastic scattering
Wille, H *-C; Hermann, Raphaël ULg; Sergueev, I. et al

in Physical Review. B, Condensed Matter and Materials Physics (2007), 76(14),

The specific lattice dynamic properties of antimony in the unfilled CoSb3 and filled EuFe4Sb12 skutterudites have been determined by nuclear inelastic scattering at the Sb-121 nuclear resonance energy of ... [more ▼]

The specific lattice dynamic properties of antimony in the unfilled CoSb3 and filled EuFe4Sb12 skutterudites have been determined by nuclear inelastic scattering at the Sb-121 nuclear resonance energy of 37.1298(2) keV with a 4.5 meV high-resolution backscattering sapphire monochromator. The Sb partial vibrational density of states (DOS) shows a maximum centered at 17 and 16 meV in CoSb3 and EuFe4Sb12, respectively. The difference between the Sb DOSs of CoSb3 and EuFe4Sb12 reveals that upon filling there is a transfer of 10% of the vibrational states toward lower energy. Further, a likely indication of the coupling between the guest and the host lattice in rattler systems is observed, a coupling that is required to reduce the lattice thermal conductivity. [less ▲]

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See detailAntimony-121 Mossbauer spectral study of alpha-Zn4Sb3
Hermann, Raphaël ULg; Grandjean, Fernande ULg; Chen, Tsi*-Chi et al

in Inorganic Chemistry (2007), 46(3), 767-770

The Mossbauer spectra of alpha-Zn4Sb3, a compound that is best formulated as alpha-Zn13Sb10 or (Zn2+)(13)(Sb3-)(6)(Sb-2(4-))(2), have been measured between 5 and 120 K. The resulting six spectra have been ... [more ▼]

The Mossbauer spectra of alpha-Zn4Sb3, a compound that is best formulated as alpha-Zn13Sb10 or (Zn2+)(13)(Sb3-)(6)(Sb-2(4-))(2), have been measured between 5 and 120 K. The resulting six spectra have been simultaneously fit with two components in the ratio of 3:2 corresponding to the Sb3- and Sb2- ions identified in this valence semiconductor. The fits yield temperature independent isomer shifts of -8.17(2) and -9.73(2) mm/s and quadrupole interactions of -4.9(2) and 0 mm/s for the Sb3- and Sb2- ions, respectively; the corresponding Mossbauer temperatures are 197(5) and 207(5) K, temperatures that are lower than the Debye temperature of beta-Zn4Sb3. The isomer shifts correspond to electronic configurations between 5s(2)5p(6) and 5s(1.75)5p(4.01) for the Sb3- ions and between 5s(2)5p(5) and 5s(1.80)5p(3.38) for the Sb2- ions, configurations that are in good agreement with the expected configurations for this valence semiconductor and with the results of band structure calculations. [less ▲]

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See detailAntimony-121 Mossbauer spectral study of the Eu14MnSb11 and Yb14MnSb11 Zintl compounds
Hermann, Raphaël ULg; Grandjean, Fernande ULg; Kafle, D. et al

in Inorganic Chemistry (2007), 46(25), 10736-10740

The antimony-121 Mossbauer spectra of the Yb14MnSb11 and Eu14MnSb11 Zintl compounds have been measured between 2 or 5 and 300 K. The resulting three-dimensional arrays of the spectral counts, velocity ... [more ▼]

The antimony-121 Mossbauer spectra of the Yb14MnSb11 and Eu14MnSb11 Zintl compounds have been measured between 2 or 5 and 300 K. The resulting three-dimensional arrays of the spectral counts, velocity, and temperature have been simultaneously fit with a minimum number of free parameters. These fits yield a 0 Kelvin transferred hyperfine field of 2.9(2) T, a Curie temperature of 57(3) K, and a Mossbauer temperature of 182(2) K for Yb14MnSb11; in this case the transferred field arises solely from the ordering of Mn2+. Because Eu14MnSb11 has both Eu2+ and Mn2+ ions that are magnetically ordered, its antimony-121 Mossbauer spectra are more complex and reveal two magnetic transitions, the first at 92(1) K resulting from the ordering of the Mn 2+ ions and the second at 9.5(1.0) K resulting from the ordering of the Eu2+ ions; the corresponding 0 Kelvin transferred hyperfine fields are 1.3(1) and 3.7(1) T. The antimony-121 isomer shifts yield electronic configurations of 5s(1.74)5p(4.28) and 5s(1.74)5p(4.19) for the average antimony anion in Yb14MnSb11 and Eu14MnSb11, respectively. [less ▲]

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