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See detailIdentification of specific bovine blood biomarkers with a non-targeted approach using HPLC tandem mass spectrometry
Lecrenier, M.C.; Marbaix, H.; Dieu, M. et al

in Belgian Journal of Food Chemistry and Biotechnology (2016)

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See detailIdentification of specific reachable molecular targets in human breast cancer using a versatile ex vivo proteomic method
Castronovo, Vincenzo ULiege; Kischel, Philippe ULiege; Guillonneau, Francois et al

in Proteomics (2007), 7(8), 1188-1196

Targeting of tumoral tissues is one of the most promising approaches to improve both the efficacy and safety of anticancer treatments. The identification of valid targets, including proteins specifically ... [more ▼]

Targeting of tumoral tissues is one of the most promising approaches to improve both the efficacy and safety of anticancer treatments. The identification of valid targets, including proteins specifically and abundantly expressed in cancer lesions, is of utmost importance. Despite state-of-the-art technologies, the discovery of cancer-associated target proteins still faces the limitation, in human tissues, of antigen accessibility to suitable high-affinity ligands such as human mAb bound to bioactive molecules. Terminal perfusion of tumor-bearing mice or ex vivo perfusion of human cancer-bearing organs with a reactive biotin ester solution has successfully led to the identification of novel accessible biomarkers. This methodology is however restricted to perfusable organs, and excludes most of the tissues of interest to targeted therapies, e.g. primary breast cancer and metastases. Herein, we report on the development of a new chemical proteomic method that bypasses the perfusion step and thus offers the potential to identify accessible molecular targets in virtually all types of animal and human tissues. We have validated our new procedure by identifying biomarkers selectively expressed in human breast carcinoma. Overall, this powerful technology may lay the ground not only for custom-made therapies in cancer, but also for the development of therapies that need to be selectively delivered in a specific tissue. [less ▲]

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See detailIdentification of sprinkling storage facilities for windblown timber using a GIS-based modeling approach
Riguelle, Simon ULiege; Jourez, Benoît ULiege; Hebert, Jacques ULiege et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2017), 21(2),

After catastrophic storm events, the storage of windblown timber is an effective measure for mitigating economic losses in the forest sector by preventing wood decay, protecting stands from secondary ... [more ▼]

After catastrophic storm events, the storage of windblown timber is an effective measure for mitigating economic losses in the forest sector by preventing wood decay, protecting stands from secondary damage, and regulating the timber supply in the middle and long term. In this study, we first propose a GIS-based methodology for identifying suitable sprinkling storage terminals in Wallonia (Belgium). In addition, we suggest an approach for building a coherent regional network as well as methods for selecting and activating terminals within this network after a storm, depending on the severity and distribution of the damage. The GIS-based approach was used to crosscheck technical requirements related to sprinkling storage according to operational and environmental constraints in the Ardenne, which is the most forested sub-region of Wallonia. A three-step process was employed to identify suitable areas. Nine procurement areas were also delineated according to the regional forest inventory plots as a reference for choosing the terminals that should be included in the regional network and activated after the storm. We generated and evaluated 96 scenarios. In the second step, a network of 30 terminals was suggested, which corresponded to a storage capacity of 4 million m³. This network could facilitate the flexibility in the strategic management of storage after huge storms. The procurement area approach also helps addressing routing and transportation issues in a simple way. The GIS approach facilitates the selection of sprinkling storage terminals, but field validation and enhanced collaboration between public and private landowners and forest owners would still be needed. [less ▲]

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See detailIdentification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma.
Kischel, Philippe; Waltregny, David ULiege; Greffe, Yannick et al

in Proteome Science (2011), 9(1), 63

ABSTRACT: Hodgkin lymphoma (HL) represents a category of lymphoid neoplasms with unique features, notably the usual scarcity of tumour cells in involved tissues. The most common subtype of classical HL ... [more ▼]

ABSTRACT: Hodgkin lymphoma (HL) represents a category of lymphoid neoplasms with unique features, notably the usual scarcity of tumour cells in involved tissues. The most common subtype of classical HL, nodular sclerosis HL, characteristically comprises abundant fibrous tissue stroma. Little information is available about the protein composition of the stromal environment from HL. Moreover, the identification of valid protein targets, specifically and abundantly expressed in HL, would be of utmost importance for targeted therapies and imaging, yet the biomarkers must necessarily be accessible from the bloodstream. To characterize HL stroma and to identify potentially accessible proteins, we used a chemical proteomic approach, consisting in the labelling of accessible proteins and their subsequent purification and identification by mass spectrometry. We performed an analysis of potentially accessible proteins in lymph node biopsies from HL and reactive lymphoid tissues, and in total, more than 1400 proteins were identified in 7 samples. We have identified several extracellular matrix proteins overexpressed in HL, such as versican, fibulin-1, periostin, and other proteins such as S100-A8. These proteins were validated by immunohistochemistry on a larger series of biopsy samples, and bear the potential to become targets for antibody-based anti-cancer therapies. [less ▲]

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See detailIdentification of structural determinants controlling human and mouse stromelysin-3 proteolytic activities
Noël, Agnès ULiege; Santavicca, M.; Stoll, I. et al

in Journal of Biological Chemistry (1995), 270

Matrix metalloproteinases (matrixins) constitute a group of extracellular proteinases belonging to the metzincin superfamily. They are involved in both physiological and pathological tissue remodeling ... [more ▼]

Matrix metalloproteinases (matrixins) constitute a group of extracellular proteinases belonging to the metzincin superfamily. They are involved in both physiological and pathological tissue remodeling processes, including those associated with cancer progression. Stromelysin-3, which is expressed in most invasive human carcinomas, is a matrix metalloproteinase with unusual functional properties. In particular, its mature form does not cleave any of the major extracellular matrix components. To define critical structural determinants involved in controlling stromelysin-3 proteolytic activity, we have used site-directed mutagenesis. We show that the deletion of at least 175 C-terminal amino-acids is sufficient to endow mouse stromelysin-3 with activities against casein, laminin, and type IV collagen. In the case of the human enzyme, however, a further and single Ala-235 Pro substitution is necessary to observe similar activities. Ala-235, which characterizes human stromelysin-3 among matrixins, is located immediately after the C terminus of the “Met-turn,” which forms a hydrophobic basis for the catalytic zinc atom in the metzincin family. We conclude that human stromelysin-3 has gained specific functional properties during evolution by amino acid substitution in the catalytic zinc environment, and that it represents an attractive target for specific inhibitors that may be used to prevent cancer progression. [less ▲]

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See detailIdentification of Structural Free-play Non-linearities using the Non-Linear Resonant Decay Method
Yang, Z.; Dimitriadis, Grigorios ULiege; Vio, Gareth A. et al

in Proceedings of the 2006 International Conference on Noise and Vibration Engineering (2006, September)

Structural non-linearities are becoming of increasing importance in determining the performance of a range of vibrating mechanical structures. As a consequence, the identification of systems with non ... [more ▼]

Structural non-linearities are becoming of increasing importance in determining the performance of a range of vibrating mechanical structures. As a consequence, the identification of systems with non-linearities is starting to become a necessary part of vibration testing procedures. NL-RDM (Non-Linear Resonant Decay Method) is an approach for the identification of non-linear multi-degree of freedom systems in modal space on a mode by mode basis, using an appropriated sine excitation to isolate modes or groups of modes. However, the application of NL-RDM to a multi-degree of freedom system with a discontinuous free-play non-linearity has not been attempted yet, except to treat it using high order polynomial terms. The difficulty of using NL-RDM when seeking a discontinuous free-play model lies in the choice of the so-called underlying linear model to be used in uncoupling the linear equations. In this paper, a simulated two degree of freedom lumped parameter system with a free-play non-linearity [less ▲]

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See detailIdentification of Suitable Steels : Chapter 3
Rassili, Ahmed ULiege; Robelet, Marc; Lecomte-Beckers, Jacqueline ULiege

in Rassili, Ahmed; Atkinson, Helen (Eds.) Thixoforming steels (2010)

A range of steels is normally industrially available. The question here is to identify those which are most suitable for thixoforming and to adapt the composition to maximise the chances of success. In ... [more ▼]

A range of steels is normally industrially available. The question here is to identify those which are most suitable for thixoforming and to adapt the composition to maximise the chances of success. In this chapter, the influence of alloying elements on the solidus and liquidus temperatures and on the thixoforming window is identified. Some case studies for low and medium carbon steels are given. Thermophysical properties are important for processing and some information is given on the characterisation of these. High carbon chromium steels are another class of steels which are potentially of use. The effect of modifying the Mn and Si content of such steels is described. Thermodynamic prediction is a powerful tool and results from such studies are given. There is then an analysis of the influence of micro-segregation, which results from the original hot rolling, and its interaction with heating speed during the reheating for thixoforming. [less ▲]

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See detailIdentification of surf actin producing strains in Soumbala and Bikalga fermented condiments using polymerase chain reaction and matrix assisted laser desorption/ionization-mass spectrometry methods
Savadogo, A; Tapi, Arthur; Chollet, Marlène et al

in International Journal of Food Microbiology (2011)

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See detailIdentification of tenuis of four French Polynesian Carapini (Carapidae : Teleostei)
Parmentier, Eric ULiege; Lo-Yat, A.; Vandewalle, Pierre ULiege

in Marine Biology (2002), 140(3), 633-638

Four species of adult Carapini (Carapidae) occur on Polynesian coral reefs: Encheliophis gracilis, Carapus boraborensis, C. homei and C. mourlani. Samples collected in Rangiroa and Moorea allowed us to ... [more ▼]

Four species of adult Carapini (Carapidae) occur on Polynesian coral reefs: Encheliophis gracilis, Carapus boraborensis, C. homei and C. mourlani. Samples collected in Rangiroa and Moorea allowed us to obtain different tenuis (larvae) during their settlement phases or directly inside their hosts. These were separated into four lots on the basis of a combination of pigmentation, meristic, morphological, dental and otolith (sagittae) features. Comparison of these characters with those of the adults allows, for the first time, taxonomic identification of these tenuis-stage larvae. [less ▲]

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See detailIdentification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein.
Clausse, Nathalie; Jackers, Pascale ULiege; Jares, P. et al

in DNA & Cell Biology (1996), 15(12), 1009-23

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents ... [more ▼]

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents intriguing multifunctional properties because it has been characterized as the precursor of the metastasis-associated 67-kD laminin receptor (67LR) and as a cytoplasmic ribosomal-associated protein. The isolation of the 37LRP/p40 gene is a prerequisite for identifying the molecular mechanisms responsible for the constant up-regulation of the 67LR expression in cancer cells. To date, the active 37LRP/p40 gene has never been identified in any species due to the existence of multiple pseudogenes in most vertebrates genomes. In this study, we report for the first time the gene structure and potential regulatory sequences of the 37 LRP/p40 gene. The chicken genome was selected to undergo this characterization because it is the only known vertebrate that bears a single 37 LRP/p40 gene copy. The 37 LRP/p40 active gene is composed of 7 exons and 6 introns and bears features characteristic of a ribosomal protein gene. It does not bear a classical TATA box and it exhibits several transcription initiation sites as demonstrated by RNase protection assay and primer extension. Analysis of potential regulatory regions suggests that gene expression is driven not only by the 5' genomic region but also by the 5' untranslated and intron 1 sequences. On the basis of gene structure and extensive protein evolutionary study, we found that the carboxyterminal domain of the protein is a conserved lock-and-key structure/function domain that could be involved in the biosynthesis of the higher-molecular-weight 67-kD laminin receptor in vertebrates, whereas the central core of the protein would be responsible for the ribosome associated function. The first identification of the active 37LRP/p40 gene presented in this study is a critical step toward the isolation of the corresponding human gene and the understanding of the molecular mechanisms involved in the up-regulation of its expression during tumor invasion and metastasis. [less ▲]

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See detailIdentification of the bacterial symbionts of the banana aphid Pentalonia nigronervosa and characterization of their roles
De Clerck, Caroline ULiege

Doctoral thesis (2015)

Les insectes sont caractérisés par une incroyable capacité d’adaptation à de nombreux environnements et modes d’alimentation, qui est liée au fait que la majorité des insectes (voire tous) hébergent des ... [more ▼]

Les insectes sont caractérisés par une incroyable capacité d’adaptation à de nombreux environnements et modes d’alimentation, qui est liée au fait que la majorité des insectes (voire tous) hébergent des symbiontes intra ou extracellulaires. Ces associations symbiotiques sont très diverses et peuvent aller d’un mutualisme obligatoire à un parasitisme facultatif. Les pucerons, en particulier, sont connus pour vivre en symbiose avec au moins une bactérie obligatoire qui leur fournit les nutriments essentiels absents du phloème dont ils se nourrissent : Buchnera aphidicola. La plupart des symbiontes d’insectes étant incultivables, les technologies moléculaires modernes se sont avérées des outils puissants dans la compréhension des interactions hôte-microorganisme et la caractérisation du rôle joués par ces symbiontes. Les études génomiques ont mené à la découverte des plus petits génomes bactériens jamais observés, et ont permis une étude plus poussée des symbiontes secondaires et de leur évolution. Pentalonia nigronervosa, le puceron noir du bananier, lest le vecteur principal du virus des sommets touffus du bananier, l’un des virus les plus dommageables en culture bananière. Très peu d’études sont néanmoins disponibles sur ce vecteur et rien n’était connu sur la population symbiotique hébergée par cet insecte jusqu’à cette étude. Dans cette thèse, nous avons découvert que le puceron du bananier contient deux symbiontes : B. aphidicola (BPn) et Wolbachia sp. (wPn); et cela dans tous les pucerons testés, quelle que soit leur origine géographique. La présence de Buchnera était attendue (99,9% de toutes les espèces de pucerons contiennent ce symbionte), mais la détection systématique de Wolbachia nous fait nous interroger sur le rôle que cette bactérie pourrait jouer pour l’insecte hôte, ainsi que sur la raison de sa présence. Wolbachia n’est en effet que rarement détectée chez les pucerons et est surtout connue pour être un parasite qui manipule le système reproducteur de ces hôtes. Malgré tout, des études ont montré que cette bactérie pouvait agir comme mutualiste pour certains insectes, ce qui pourrait expliquer sa présence dans le puceron du bananier. Le second objectif de cette étude a donc été de mieux comprendre le rôle joué par les deux symbiontes de P. nigronervosa. Pour ce faire, différentes techniques complémentaires ont été utilisées. La microinjection d’antibiotiques a été utilisée afin d’éliminer sélectivement wPn et de voir l’effet de cette élimination sur l’hôte. Nous avons observé que l’élimination de wPn donnait systématiquement lieu à la mort du puceron. Un séquençage haut débit de l’hémolymphe (contenant des bacteriocytes) d’un puceron a été réalisé et nous a permis d’identifier et d’annoter 587 et 250 gènes pour wPn et BPn respectivement. L’analyse de ces gènes nous permet d’émettre l’hypothèse que les deux bactéries collaborent pour la production de plusieurs nutriments essentiels pour l’hôte. La situation est surtout frappante dans le cas de la lysine et de la riboflavine, qui sont habituellement produits uniquement par Buchnera chez les autres espèces de pucerons. Dans le cas du puceron du bananier, les gènes impliqués dans les voies de production de ces métabolites sont répartis entre les génomes des deux bactéries, les rendant ainsi toutes les deux essentielles à la survie du puceron hôte. Enfin, une localisation par fluorescence in situ des deux symbiontes a montré qu’ils étaient tous deux localisés dans les bacteriocytes, rendant les échanges de précurseurs et métabolites possibles. En conclusion, nos résultats suggèrent que le puceron du bananier héberge une co-symbiose obligatoire entre B. aphidicola et Wolbachia, les deux bactéries agissant de concert dans l’approvisionnement de l’hôte en nutriments essentiels. [less ▲]

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See detailIdentification of the bovine leukemia virus transactivating protein (p34x).
Willems, Luc ULiege; Kettmann, Richard ULiege; Portetelle, Daniel ULiege et al

in Haematology and Blood Transfusion (1987), 31(1), 482-7

Detailed reference viewed: 14 (8 ULiège)
See detailIdentification of the cis element recognized by a transcription factor specific to the tumor BT-474 mammary cells.
Grooteclaes, Madeleine; Pasleau, Françoise ULiege; Hodzic, Didier et al

Conference (1996, February 03)

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See detailIdentification of the concepts at stake during a consultation. Clinical exercises
Jamoulle, Marc ULiege

E-print/Working paper (2015)

During a consultation between a patient and a doctor, symptoms, process and health problems are at stake and could be identified and retrieved through the use of ICPC.(refer to www.ph3c.org) Specific ... [more ▼]

During a consultation between a patient and a doctor, symptoms, process and health problems are at stake and could be identified and retrieved through the use of ICPC.(refer to www.ph3c.org) Specific training and managerial competences of the doctor could be identified and explained through the Q-Codes Together ICPC and Q-Codes form 3CGP ; Core Content Classification in General Practice/ Family Medicine The concepts identified in ICPC and Q-Codes could be useful to understand and teach the content of consultation in family medicine. [less ▲]

Detailed reference viewed: 39 (8 ULiège)