Antibiotiques et Probiotiques dans la sinusite chronique.
POIRRIER, Anne-Lise ;
Scientific conference (2015)Detailed reference viewed: 15 (0 ULg)
Les antibiotiques topiques en Europe: résistances insulaires et indifférence continentale?
Henry, Frédérique ; ; Franchimont, Claudine et al
in Dermatologie Actualité (2005), 92Detailed reference viewed: 17 (1 ULg)
Antibodies against bovine herpesvirus 4 are highly prevalent in wild African buffaloes throughout eastern and southern Africa
Dewals, Benjamin G ; Gillet, Laurent ; et al
in Veterinary Microbiology (2005), 110(3-4), 209-220
Bovine herpesvirus 4 (BoHV-4) has been isolated from cattle throughout the world. Interestingly, a survey of wild African buffaloes mainly from the Maasai Mara Game Reserve in Kenya revealed that 94% of ... [more ▼]
Bovine herpesvirus 4 (BoHV-4) has been isolated from cattle throughout the world. Interestingly, a survey of wild African buffaloes mainly from the Maasai Mara Game Reserve in Kenya revealed that 94% of the animals tested had anti-BoHV-4 antibodies [Rossiter, P.B., Gumm, I.D., Stagg, D.A., Conrad, PA., Mukolwe, S., Davies, F.G., White, H., 1989. Isolation of bovine herpesvirus-3 from African buffaloes (Syncerus caffer). Res. Vet. Sci. 46, 337-343]. These authors also proposed that the serological antigenic relationship existing between BoHV-4 and alcelaphine herpesvirus I (A1HV-1) could confer to BoHV-4 infected buffaloes a protective immune response against lethal A1HV-1 infection. In the present study, we addressed two questions related to Rossiter et al. paper. Firstly, to investigate the role of the African buffalo as a natural host species of BoHV-4, the seroprevalence of anti-BoHV-4 antibodies was analysed in wild African buffaloes throughout eastern and southern Africa. A total of 400 sera was analysed using two complementary immunofluorescent assays. These analyses revealed that independently of their geographical origin, wild African buffaloes exhibit a seroprevalence of anti-BoHV-4 antibodies higher than 68%. This result is by far above the seroprevalence generally observed in cattle. Our data are discussed in the light of our recent phylogenetic study demonstrating that the BoHV-4 Bo17 gene has been acquired from a recent ancestor of the African buffalo. Secondly, we investigated the humoral antigenic relationship existing between BoHV-4 and A1HV-1. Our results demonstrate that among the antigens expressed in A1HV-1 infected cells, epitope(s) recognised by anti-BoHV-4 antibodies are exclusively nuclear, suggesting that the putative property of BoHV-4 to confer an immune protection against A1HV-1 relies on a cellular rather than on a humoral immune response. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]Detailed reference viewed: 41 (8 ULg)
Antibodies against salmon calcitonin: absence of blocking properties
Reginster, Jean-Yves ; ; et al
in Journal of Bone and Mineral Research (1989), 4(S1), 948Detailed reference viewed: 7 (1 ULg)
Antibodies against vaccinia virus do not neutralize extracellular enveloped virus but prevent virus release from infected cells and comet formation
Vanderplasschen, Alain ; ;
in Journal of General Virology (The) (1997), 78(Pt 8), 2041-2048Detailed reference viewed: 10 (2 ULg)
Antibodies Dynamics in the Broiler Digestive Tract after Salmonella Typhimurium Oral Challenge
Marcq, Christopher ; ; Thewis, André et al
in Duclos, Michel; Nys, Yves (Eds.) XIIIth European Poultry Conference: Program & Book of Abstracts, World's Poultry Science Journal, Volume 66, Supplement (2010, August)
This study aimed to provide directions for Salmonella challenge experiment conception by offering a better understanding of the relationship between age and mucosal immune responsiveness of chicken ... [more ▼]
This study aimed to provide directions for Salmonella challenge experiment conception by offering a better understanding of the relationship between age and mucosal immune responsiveness of chicken. Intestinal maternal immunity of Salmonella-free chicks was monitored by ELISA analyses at 2, 9 and 16 days of age. At 21 days of age, chicks were orally inoculated with Salmonella Typhimurium. Three inoculum doses (3 x 10E3, 3 x 10E6, 3 x 10E9 cfu/bird) and an uninfected control were then compared concerning mucosal immune status of the small intestine and cecum. Results suggest that difficulties encountered to infect very young chicks can be related, at least partly, to maternal immunity. The relatively low level of intestinal immune defences observed thereafter could indicate a right time to perform an experimental infection of birds and to maximize the spreading of the pathogen during challenge experiment. After infection of 3-week-old chickens, the mucosal immune response was rapid with increased anti-Salmonella Typhimurium IgA titers. There was a linear relationship between specific IgA levels in intestinal and cecal secretions and the challenge dose initially inoculated. [less ▲]Detailed reference viewed: 130 (15 ULg)
Antibodies to laminin in Chagas' disease
; ; et al
in Journal of Experimental Medicine (1982), 155(4), 1161-71
We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues ... [more ▼]
We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite. [less ▲]Detailed reference viewed: 17 (2 ULg)
Antibodies to laminin in preeclampsia.
Foidart, Jean-Michel ; ; et al
in Kidney International (1986), 29(5), 1050-57
Laminin is a large basement membrane glycoprotein localized in the trophoblast, glomerular basement membrane and in the mesangial matrix of human glomeruli. It promotes the attachment of epithelial cells ... [more ▼]
Laminin is a large basement membrane glycoprotein localized in the trophoblast, glomerular basement membrane and in the mesangial matrix of human glomeruli. It promotes the attachment of epithelial cells to basement membrane collagen. We have found that 14 sera from 52 patients with severe preeclampsia or eclampsia contain IgG and IgM antibodies which react with placental and kidney basement membranes. These antibodies were specific for laminin and did not react with other basement membrane proteins. They were able to fix complement. They have been demonstrated by radial immunodiffusion, radioimmunoassay and immunofluorescence blocking studies. In primary cultures they were shown to impair the attachment of trophoblast cells to basement membrane collagen. High levels of circulating immune complexes were detected only in sera from preeclamptic patients with circulating antibodies to laminin. The auto-antibodies to laminin could play a major role in the pathogenesis of severe preeclampsia by impairing the attachment of trophoblast cells to placental basement membranes and by fixation to the glomerular basement membranes and mesangial matrix. [less ▲]Detailed reference viewed: 14 (0 ULg)
Antibodies to mouse mammary tumor virus (MMTV) antigens to gp52 and p28 in three lines of mice with different infectious status
; Sadzot, Catherine ; Vaira, Dolorès et al
Poster (1984)Detailed reference viewed: 9 (2 ULg)
Antibodies to purified renal tubular epithelial antigens contain activity against laminin, fibronectin, and type IV collagen.
; ; et al
in Laboratory Investigation : Journal of Technical Methods & Pathology (1988), 58(3), 278-86
Antibodies directed against tubular brush border antigens (RTE) are used to induce heterologous immune-complex nephritis. Among these antigens a glycoprotein with a molecular weight of 330 kilodaltons ... [more ▼]
Antibodies directed against tubular brush border antigens (RTE) are used to induce heterologous immune-complex nephritis. Among these antigens a glycoprotein with a molecular weight of 330 kilodaltons (gp330) has been shown to be of pathogenetic significance. We investigated whether antibodies other than those directed against gp330 are present in anti-RTE and whether they play a pathogenetic role. By using enzyme-linked immunosorbent assay techniques and Western blotting, we investigated polyclonal antibodies directed not only against crude RTE but also against RTEgp, a purified glycoprotein fraction of RTE, with respect to activity against glomerular basement membrane (GBM) components laminin, fibronectin, and type IV collagen. Both antibody preparations showed reactivity predominantly to the 220 kilodaltons subunit of laminin. Lower but nevertheless distinct reactivity to fibronectin and type IV collagen was also found. The antibody fraction directed against components of the GBM, which was isolated from anti-RTE IgG by affinity chromatography, showed linear binding to the GBM in indirect immunofluorescence studies. Injection of these antibodies into the renal artery also led to linear binding to the GBM with linear deposition of complement factors 3 and 9 and induced a weak and transient proteinuria. Immunoelectron microscopy revealed binding of the antibodies to glomerular epithelial and endothelial cell surfaces adjacent to the GBM. Injection of anti-RTE antibody absorbed to GBM components resulted in binding of antibodies and complement factors 3 and 9 in a fine granular pattern along the GBM, whereas injection of unabsorbed anti-RTE led to a course granular pattern. We conclude that the presence of antibodies (cross-)reacting with laminin, fibronectin, and type IV collagen in anti-RTE antibody has pathogenetic effects and could explain differences in pathogenicity between monospecific anti-gp330 antibody and polyclonal anti-RTE antibody. [less ▲]Detailed reference viewed: 6 (0 ULg)
Antibodies to Type II Collagen in Relapsing Polychondritis
Foidart, Jean-Michel ; ; et al
in New England Journal of Medicine [=NEJM] (1978), 299
Relapsing polychondritis is a disorder of unknown cause characterized by the destruction of cartilage. To test the hypothesis that immunologic mechanisms are involved in the pathogenesis of relapsing ... [more ▼]
Relapsing polychondritis is a disorder of unknown cause characterized by the destruction of cartilage. To test the hypothesis that immunologic mechanisms are involved in the pathogenesis of relapsing polychondritis, we analyzed the serum of 15 patients for the presence of antibodies to cartilage. Antibodies to Type II (cartilage) collagen were found in the serum of five patients at the time of acute symptoms. No antibodies were detected either to cartilage proteoglycan or to other collagen types. The antibodies were detected at the onset of the disease and their titers appeared to correlate with severity of disease. Circulating immune complexes were also detected in the serum of these patients. Our findings support an immunologic involvement in this condition. [less ▲]Detailed reference viewed: 24 (0 ULg)
Antibodies to varicella-zoster virus modulate antigen distribution but fail to induce viral persistence in vitro.
Sadzot-Delvaux, Catherine ; ; et al
in Journal of Virology (1992), 66(12), 7499-504
Varicella-zoster virus (VZV) persists in human sensory ganglia. One of the hypotheses to explain the induction or the maintenance of VZV latency is that it could be promoted by the immune response itself ... [more ▼]
Varicella-zoster virus (VZV) persists in human sensory ganglia. One of the hypotheses to explain the induction or the maintenance of VZV latency is that it could be promoted by the immune response itself. It is known that in the case of viruses which bud off the infected cell membrane, virus-specific antibodies can induce antigenic modulation, i.e., spatial redistribution of viral antigens and modulation of their synthesis. To determine whether antigenic modulation occurs during VZV infection in vitro and could possibly be involved in viral persistence, we have grown infected cells in the presence of anti-VZV antibodies either transiently or permanently. The distribution of immune complexes and viral proteins was then analyzed. In transient immunomodulation experiments, the distribution of one or more viral antigens was modified not only in the cytoplasmic membranes but also in the cytoplasm and nucleoplasm of infected cells. When infected cells were kept permanently in the presence of antibodies, the same pattern of redistribution of immune complexes was observed and the localization of internal viral glycoproteins was significantly modified. However, antibodies did not prevent the lytic effect of infection; they altered neither the infectious virus yield nor the Western immunoblot pattern of viral proteins, suggesting that immunomodulation is not the primary effector of viral persistence. [less ▲]Detailed reference viewed: 25 (11 ULg)
Antibodies to varicella-zoster virus modulate antigen distribution in cultured infected cells
; Sadzot-Delvaux, Catherine ; Merville, Marie-Paule et al
in Archives Internationales de Physiologie et de Biochimie (1989), 97Detailed reference viewed: 13 (3 ULg)
Antibody evasion by a gammaherpesvirus o-glycan shield.
Machiels, Bénédicte ; Lété, Céline ; Guillaume, Antoine et al
in PLoS Pathogens (2011), 7(11), 1002387
All gammaherpesviruses encode a major glycoprotein homologous to the Epstein-Barr virus gp350. These glycoproteins are often involved in cell binding, and some provide neutralization targets. However, the ... [more ▼]
All gammaherpesviruses encode a major glycoprotein homologous to the Epstein-Barr virus gp350. These glycoproteins are often involved in cell binding, and some provide neutralization targets. However, the capacity of gammaherpesviruses for long-term transmission from immune hosts implies that in vivo neutralization is incomplete. In this study, we used Bovine Herpesvirus 4 (BoHV-4) to determine how its gp350 homolog - gp180 - contributes to virus replication and neutralization. A lack of gp180 had no impact on the establishment and maintenance of BoHV-4 latency, but markedly sensitized virions to neutralization by immune sera. Antibody had greater access to gB, gH and gL on gp180-deficient virions, including neutralization epitopes. Gp180 appears to be highly O-glycosylated, and removing O-linked glycans from virions also sensitized them to neutralization. It therefore appeared that gp180 provides part of a glycan shield for otherwise vulnerable viral epitopes. Interestingly, this O-glycan shield could be exploited for neutralization by lectins and carbohydrate-specific antibody. The conservation of O-glycosylation sites in all gp350 homologs suggests that this is a general evasion mechanism that may also provide a therapeutic target. [less ▲]Detailed reference viewed: 103 (29 ULg)
Antibody evasion by a gammaherpesvirus O-glycan shield.
Machiels, Bénédicte ; Gillet, Laurent
Conference (2011, November)Detailed reference viewed: 11 (1 ULg)
Antibody evasion by the N terminus of murid herpesvirus-4 glycoprotein B.
Gillet, Laurent ;
in EMBO Journal (2007), 26(24), 5131-42
Herpesviruses characteristically transmit infection from immune hosts. Although their success in escaping neutralization by pre-formed antibody is indisputable, the underlying molecular mechanisms remain ... [more ▼]
Herpesviruses characteristically transmit infection from immune hosts. Although their success in escaping neutralization by pre-formed antibody is indisputable, the underlying molecular mechanisms remain largely unknown. Glycoprotein B (gB) is the most conserved component of the herpesvirus entry machinery and its N terminus (gB-NT) is a common neutralization target. We used murid herpesvirus-4 to determine how gB-NT contributes to the virus-antibody interaction. Deleting gB-NT had no obvious impact on virus replication, but paradoxically increased virion neutralization by immune sera. This reflected greater antibody access to neutralization epitopes on gH/gL, with which gB was associated. gB-NT itself was variably protected against antibody by O-linked glycans; on virions from epithelial cells it was protected almost completely. gB-NT therefore provides a protective and largely protected cover for a vulnerable part of gH/gL. The conservation of predicted glycosylation sites in other mammalian herpesvirus gB-NTs suggests that this evasion mechanism is widespread. Interestingly, the gB-NT glycans that blocked antibody binding could be targeted for neutralization instead by a lectin, suggesting a means of therapeutic counterattack. [less ▲]Detailed reference viewed: 36 (6 ULg)
Antibody formation and plasma profiles of insulin-like growth factor-I (IGF-I) and IGF-binding proteins in growing heifers after sustained-release exogenous BST administration
; Portetelle, Daniel ; et al
in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1998), 2(special issue), 45Detailed reference viewed: 12 (1 ULg)
Antibody formation and plasma profiles of insulin-like growth factor-I (IGF-I) and IGF-binding proteins in growing heifers after sustained-release exogenous BST administration.
; Portetelle, Daniel ; et al
in 3° Internat. Conf. farm Anim. Endocrinol. (1998)Detailed reference viewed: 5 (0 ULg)
Antibody production by injection of living cells expressing non self antigens as cell surface type II transmembrane fusion protein.
; Gillet, Laurent ; SCHROEDER, Hélène et al
in Journal of immunological methods (2011)
Antigen expression and purification are laborious, time consuming and frequently difficult steps in the process of antibody production. In the present study, we developed a method avoiding these two steps ... [more ▼]
Antigen expression and purification are laborious, time consuming and frequently difficult steps in the process of antibody production. In the present study, we developed a method avoiding these two steps. This method relies on the injection of histocompatible living cells stably expressing the antigen as a cell surface type II transmembrane fusion protein. A vector, nicknamed pCD1-CD134L, was constructed to express the antigen fused at the carboxyterminal end of the human CD134 ligand (CD134L) type II transmembrane protein on the surface of eucaryotic cells. This vector was shown to induce cell surface expression of epitopes from human c-Myc (soluble protein), uterogloblin-related protein 1 (secreted protein) and CD94 (type II transmembrane protein). Using this vector, we developed a method to produce antibodies without antigen production. The flowchart of this method is as follows: (i) cloning of the antigen in the pCD1-CD134L vector; (ii) production of a histocompatible cell line stably expressing the CD134L-antigen fusion protein; (iii) testing for cell surface expression of the fusion protein by targeting the CD134L carrier; and (iv) prime-boost immunisation with living cells expressing the fusion protein. This method was successfully used for production of polyclonal antibodies raised against Ixodes ricinus calreticulin (secreted protein) in mice and for production of monoclonal antibodies raised against an epitope of Vaccinia virus A56 (type I transmembrane protein) protein in rat. The present study is the first to demonstrate the use of a type II transmembrane protein as a carrier for cell surface display of antigens. [less ▲]Detailed reference viewed: 60 (21 ULg)