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See detailIdentification of accessible human cancer biomarkers using ex vivo chemical proteomic strategies
Kischel, Philippe ULg; Waltregny, David ULg; Castronovo, Vincenzo ULg

in Expert Review of Proteomics (2007), 4(6), 727-739

One promising avenue towards the development of more selective, better anticancer drugs lies in the targeted delivery of bioactive compounds to the tumor environment by means of binding molecules specific ... [more ▼]

One promising avenue towards the development of more selective, better anticancer drugs lies in the targeted delivery of bioactive compounds to the tumor environment by means of binding molecules specific for tumor-associated biomarkers. Eligibility of such markers for therapeutic ideally use three criteria: accessibility from the bloodstream; expression at sufficient level, and no (or much lower) expression in normal tissues. Most current discovery strategies (such as biomarker searching into body fluids) provide no clue as to whether proteins of interest are accessible, in human tissues, to suitable high-affinity ligands, such as systemically delivered monoclonal antibodies. To address this limitation, our group recently developed two methodologies based on chemical proteomic modifications, enabling the discovery of proteins accessible from the bloodstream and the extracellular space in human pathological tissues. In this review, we will discuss the potential benefits of these methodologies for the fast discovery of therapeutically valuable biomarkers. [less ▲]

Detailed reference viewed: 26 (4 ULg)
See detailIdentification of accessible proteins expressed in human breast cancer
Castronovo, Vincenzo ULg; Kischel, Philippe; Guillonneau, François et al

Scientific conference (2006)

Detailed reference viewed: 8 (0 ULg)
See detailIdentification of Ag-NOR proteins from isolated nucleoli of Ehrlich tumor cells
Vandelaer, M; Thiry, Marc ULg; Goessens, G

Poster (1996)

Detailed reference viewed: 3 (0 ULg)
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See detailIdentification of Alpha amylase as a new allergen of mealworm in professionally exposed patients
Debaugnies, F; Francis, Frédéric ULg; delporte, C et al

Conference (2016)

Detailed reference viewed: 17 (1 ULg)
See detailIdentification of an unique Mycobacterium bovis strain in belgian cattle
Rigouts, L.; Desmecht, M.; Dufey, J. et al

Scientific conference (2001)

Detailed reference viewed: 16 (2 ULg)
Peer Reviewed
See detailIdentification of animals treated with bovine somatotropin : a new non-isotopic detection system for bST
Baronheid, Christophe; Bertozzi, Carlo; Renaville, Robert ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2000), 4

Detailed reference viewed: 22 (0 ULg)
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See detailIdentification of animals treated with bovine somatotropin : a new non-isotopic detection system for BST
Baronheid, Christophe; Bertozzi, Carlo; Renaville, Robert ULg et al

in 1st Belgian Workshop on Animal Endocrinology (1999, October 13)

Detailed reference viewed: 5 (0 ULg)
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See detailIdentification of animals treated with bovine somatotropin: a new non-isotopic detection system for BST.
Baronheid, C.; Bertozzi, C.; Renaville, Robert ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2000), 4(1),

Detailed reference viewed: 12 (1 ULg)
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See detailIdentification of anionic selenium species in Se-rich yeast by electrospray QTOF MS/MS and hybrid linear ion trap/orbitrap MSn
Dernovics, M.; Far, Johann ULg; Lobinski, R.

in Metallomics (2009), 1(4), 317-329

An analytical approach allowing the identification Of unknown selenium metabolites ill selenium-rich yeast was described. Anion-exchange HPLC of the Se-metabolome fraction co-eluting with salts ill size ... [more ▼]

An analytical approach allowing the identification Of unknown selenium metabolites ill selenium-rich yeast was described. Anion-exchange HPLC of the Se-metabolome fraction co-eluting with salts ill size-exclusion chromatography allowed the separation of nine selenium species (excluding isomers and selenate) as monitored by inductively Coupled plasma mass spectrometry (ICP MS). The individual fractions were analyzed by electrospray QTOF MS/MS and hybrid linear ion trap/Orbitrap MSn after sample introduction by reversed-phase nanoHPLC and by hydrophilic interaction LC (HILIC), respectively. Out of the nine detected species. eight were identified oil the basis of accurate mass measurements and collision induced dissociation/fragmentation information. Seven Se-species (selenohomolanthionine, gamma-Glu-selenocystathionine, 2,3-DHP-selenocystathionine, N-acetyl-selenocystathionine, 2,3-DHP-selenohomolanthionine Se-methyl-selenoglutathione, and 2,3-DHP-Se-methylselenocysteine) were reported for the first time in Se-rich yeast. five of them have never been reported in any biological sample before. [less ▲]

Detailed reference viewed: 32 (2 ULg)
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See detailIdentification of antigens recognized in the developing mouse brain by the RC2 antibody, a marker of radial glia
Leprince, Pierre ULg; Chanas-Sacre, G.; Wattiez, R. et al

in International Journal of Developmental Neuroscience (1996), 14(Sup. 1), 84

Detailed reference viewed: 27 (8 ULg)
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See detailIdentification of antiplasmodial compounds isolated from endemic plants of Reunion Island
Ledoux, Allison ULg; Jansen, Olivia ULg; Campos, Pierre-Éric et al

Conference (2015, May)

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See detailIdentification of aphid salivary proteins: a proteomic investigation of Myzus persicae.
Harmel, Nicolas ULg; Letocart, E.; Cherqui, A. et al

in Insect Molecular Biology (2008), 17(2), 165-74

The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence ... [more ▼]

The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence reactions. Here, we focused on the salivary proteome of the green peach aphid, Myzus persicae. Proteins were either directly in-solution digested or were separated by 2D SDS-PAGE before trypsin digestion. Resulting peptides were then identified by mass spectrometry coupled with database investigations. A homemade database was constituted of expressed sequence tags from the pea aphid Acyrtosiphon pisum and M. persicae. The databases were used to identify proteins related to M. persicae with a nonsequenced genome. This procedure enabled us to discover glucose oxidase, glucose dehydrogenase, NADH dehydrogenase, alpha-glucosidase and alpha-amylase in M. persicae saliva. The presence of these enzymes is discussed in terms of plant-aphid interactions. [less ▲]

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See detailIdentification of asthma-related trans-acting epistatic eQTLs using Model-Based Multifactor Dimensionality Reduction (MB-MDR)
Bessonov, Kyrylo ULg

Poster (2013, October 22)

Epistasis is likely to underlie most complex traits, including gene expression, yet it is very difficult to detect using standard approaches. SNPs located inside a gene coding region or in its vicinity (i ... [more ▼]

Epistasis is likely to underlie most complex traits, including gene expression, yet it is very difficult to detect using standard approaches. SNPs located inside a gene coding region or in its vicinity (i.e. ≤2 Mb from each 5’ and 3’ side) can influence the corresponding gene expression levels. These expression quantitative trait loci (eQTLs) are referred to as cisSNPs. In contrast, eQTLs that are outside the aforementioned gene range can also influence the gene’s expression, in which case, they are called transSNPS to that gene. In this study we considered significant cisSNPs previously identified via generalized least squares (GLS) regression modeling. We then identified those genes transcripts whose expression is regulated by cis/transSNP interaction. In this work we aimed at identifying transcripts whose expression is regulated by a cis/transSNP interactions using Model-Based Multifactor Dimensionality Reduction (MB-MDR) [2]. This model-free approach to detect trans-epistasis involves reducing a high-dimensional GxG space to GxG factor levels that either exhibit high evidence, low evidence or no evidence at all for their association to gene expression levels of interest. Our protocol was applied on real-life data from the childhood asthma management program (CAMP) [1]. It involved coupling a traditional a priori eQTL search to an a posteriori trans-epistasis analysis to identify genetic modifiers to statistically significant cisSNPs. Such an approach allows to reveal previously unreported inter-dependencies that may be important in understanding of biological mechanisms underlying human complex diseases such as asthma. The proposed protocol identified a large number trans-epistasis gene-gene effects of eQTLs. [less ▲]

Detailed reference viewed: 38 (11 ULg)
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See detailIdentification of bacteria community associated with earthworm gut
Lemtiri, Aboulkacem ULg; Alabi, Taofic; Bodson, Bernard ULg et al

Poster (2012, July 26)

The role of earthworms in soil fertility and transformation of organic waste was regulary cited to be of first importance. Associated to these macro-invertebrates, a large diversity of micro-orgnisms are ... [more ▼]

The role of earthworms in soil fertility and transformation of organic waste was regulary cited to be of first importance. Associated to these macro-invertebrates, a large diversity of micro-orgnisms are found indirectly in their closed environment or directly in their gut. Functional aspects of these interactions and symbiosis in relation with soil characteristics and fertility rates are poorly developed. Here, the micro-organisms diversity and potential related functions of earthworm gut were investigated using a proteiomic approach for both protein and micro-organism identifications. Microbial community investigation was detected by proteomic approach based on bidimensional electrophoresis coupled with mass spectrometry using Matrix Assisted Laser Desorption Ionisation – time of flight (Maldi-Tof). Diversity of gut associated bacterial communities was discussed. Indeed, application of particular crop production practices such as crop residue management at the field level could regulate the gut bacterial communities in earthworm but also microbials in soils. Agricultural systems had to consider the microbial and associated organisms in the soil to enhance fertlility and crop production in sustainable ways. [less ▲]

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See detailIdentification of bacterial strains isolated from the traditional date product "Btana" produced in south regions of Algeria
Abekhti, Abdelkader; Taminiau, Bernard ULg; Kihal, Mabrouk et al

in Folia Microbiologica (in press)

Eleven samples of the traditional date product " Btana" prepared by direct (DBM) and indirect method (UBM) were analysed to characterize their bacterial diversity. A total of 42 representative isolates ... [more ▼]

Eleven samples of the traditional date product " Btana" prepared by direct (DBM) and indirect method (UBM) were analysed to characterize their bacterial diversity. A total of 42 representative isolates have been chosen for molecular identification. 16S rRNA gene sequencing revealed the presence of 20 species within 30.9% belonged to the genus Bacillus, 28.6% of the Staphylococcus, and Enterococcus genus. Within the minority represented species, two isolates were identified as Paenibacillus (isolated from UBM exclusively), Streptococci salivarius, Lactobacillus sakei and Klebsiella pneumoniae. Preliminary results indicate that IBM is more selective for spore former bacilli contrary to DBM that show more diversity in the bacterial flora with a prevalence of Enterococcus. API ZYM test showed that the bacilli species have a weak hydrolase activity. [less ▲]

Detailed reference viewed: 41 (7 ULg)