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See detailL’apoptose mammaire dans le sein normal et en pathologie
DESREUX, Joëlle ULg; GOFFIN, Frédéric ULg; BELIARD, Aude ULg et al

in Références en Gynécologie Obstétrique (1999), 6

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See detailApoptosis and cytolysis induced by giganteosides and hederacolchisides in HL-60 cells
Gerkens, Pascal; Dobson, Rowan ULg; tabatadze, Nino et al

in Anticancer Research (2007), 27

The viability, cytolysis and apoptosis-mediated cellular death induced by giganteosides D and E (Gig-D and Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol- A1) were analysed in HL-60 cells ... [more ▼]

The viability, cytolysis and apoptosis-mediated cellular death induced by giganteosides D and E (Gig-D and Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol- A1) were analysed in HL-60 cells. Materials and Methods: the end-point metabolic (WST1) and lactate dehydrogenase (LDH) assays were used. Cell cycle analysis and apoptosis were measured by flow cytometry, DNA laddering and caspase 3 analyses. Results: the HL-60 cell line was more sensitive to Hcol-A1 and Gig-D (IC50 3-5 ÌM) than to Gig-E and Hcol-A (IC50 8-13 ÌM; WST1 assay). This was related to LDH release. The induction of apoptosis could be detected without caspase 3 activation after 24 h of treatment. DNA fragmentation could be detected only with Gig-D. With Hcol- A1 and Gig-D, an accumulation of cells in the S-phase and an increase of cells in sub-G1 peak were observed. By the annexinV-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (AAD) assay, the majority of cells were in late apoptosis with Gig-D, and in necrosis with Hcol-A1. Conclusion: Hcol-A1 is more cytotoxic than Gig-D, followed by Gig-E and finally Hcol-A. This is related to a membrane permeabilization effect, leading to cytolysis [less ▲]

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See detailApoptosis characterizes immunological failure of HIV infected patients
Mhawej, Marie-José; Brunet-Francois, Cécile; Fonteneau, Raphaël ULg et al

in Control Engineering Practice (2009), 17(7), 798-804

This paper studies the influence of apoptosis in the dynamics of the HIV infection. A new modeling of the healthy CD4+ T-cells activation-induced apoptosis is used. The parameters of this model are ... [more ▼]

This paper studies the influence of apoptosis in the dynamics of the HIV infection. A new modeling of the healthy CD4+ T-cells activation-induced apoptosis is used. The parameters of this model are identified by using clinical data generated by monitoring patients starting Highly Active Anti-Retroviral Therapy (HAART). The sampling of blood tests is performed to satisfy the constraints of dynamical system parameter identification. The apoptosis parameter, which is inferred from clinical data, is then shown to play a key role in the early diagnosis of immunological failure. [less ▲]

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See detailApoptosis During the Development of Radiogenic Thymic Lymphomas: Effects of Treatments Inhibiting Lymphoma Development
Humblet, Chantal ULg; Denis, Ghislaine; Greimers, Roland ULg et al

in Anticancer Research (1998), 18(5A, Sep-Oct), 3469-74

INTRODUCTION: Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which preleukemic cells progressively transform into leukemic cells within an ... [more ▼]

INTRODUCTION: Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which preleukemic cells progressively transform into leukemic cells within an abnormal thymic microenvironment. A bone marrow graft or repeated cytokine injections prevent lymphoma development. We think that these treatments restore altered mechanisms controlling apoptosis. MATERIALS AND METHODS: Apoptosis was analyzed by flow cytometry in thymocytes from different groups of mice (control, preleukemic, prevented mice). RESULTS: The apoptotic rates did not change in freshly isolated thymocytes from different experimental groups. However, after culture, the level of apoptosis increased in preleukemic thymuses; and returned to normal value in cultured thymocytes from irradiated mice after lymphoma preventing treatments. Furthermore, thymic microenvironmental factors can control thymocyte apoptosis. CONCLUSION: We propose that after leukemogenic irradiation, there is an increase of cells with an activated suicide program, but that alterations of thymic environmental factors rescue them from apoptosis, allowing their further neoplastic transformation. [less ▲]

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See detailApoptosis in v-myc-transfected MSU-1.1 fibroblasts is induced by cell-matrix contact and differs from that of normal dermal fibroblasts.
Niland, S.; Cremer, A.; Herzhoff, K. et al

in In Vitro Cellular & Developmental Biology. Animal (2001), 37(9), 606-12

In order to characterize a fibroblast cell line representing normal human skin fibroblasts in three-dimensional cultures, we compared the fibroblast line MSU-1.1, derived from human foreskin and ... [more ▼]

In order to characterize a fibroblast cell line representing normal human skin fibroblasts in three-dimensional cultures, we compared the fibroblast line MSU-1.1, derived from human foreskin and immortalized by v-myc, to primary human dermal fibroblasts (NDF). Our results demonstrate that in contrast to NDF, all MSU-1.1 fibroblasts die within 3-4 d when cultured within three-dimensional contractile collagen matrices. Also, in contrast to NDF. MSU-1.1 cells die markedly in anchored collagen gels as well. Death is due to apoptosis and is attenuated by addition of antibodies against collagen-recognizing receptors alpha1beta1 and alpha2beta1. Apoptosis of NDF in collagen lattices was repressed by an inhibitor of caspase-1, which was ineffective on apoptosis of MSU-1.1. Further, apoptosis by MSU-1.l fibroblasts was also observed in anchored, i.e., restrained collagen lattices, an environment that supports proliferation of NDF. [less ▲]

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See detailApoptosis induction in human cancer cells by sungucine from Strychnos icaja root
Frederich, Michel ULg; Bentires-Ali, M.; Tits, Monique ULg et al

in Naunyn-Schmiedeberg's Archives of Pharmacology (2003), 367(3), 260-265

Sungucine (SG) and isosungucine (ISG) are bisindole alkaloids characterized by a 5'-23 link between the two parts of the compounds, which are till now specific to Strychnos icaja. In this work, SG and ISG ... [more ▼]

Sungucine (SG) and isosungucine (ISG) are bisindole alkaloids characterized by a 5'-23 link between the two parts of the compounds, which are till now specific to Strychnos icaja. In this work, SG and ISG were submitted to the NCI's in vitro 60 human tumor cell line screen, where SG showed interesting selectivity (6X) against the tested leukemia cell lines. In HL60-treated cells, apoptosis was demonstrated by observation of apoptotic bodies formation, and phosphatidylserine exposition at cell surface. In HeLa-treated cells, the analysis of cellular cycle by flow cytometry showed G1 accumulation and a small sub-G1 peak that could be related to DNA fragmentation characteristic of apoptosis. The eventual role of p53 was analyzed using wild-type HCT-116 colon cancer cells. Nevertheless, p53 and Bax expression were not modified in SG-treated cells. The cleavage of PARP by caspase-3 protease proved that apoptosis was also induced in this line. These results demonstrate that SG induces apoptosis, but also necrosis, in human cancer cell lines. [less ▲]

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See detailApoptosis of HL-60 leukemia cells induced by the bisindole alkaloids sungucine and isosungucine from Strychnos icaja
Lansiaux, A.; Bailly, Christian; Houssier, Claude ULg et al

in Planta Medica (2002), 68(7), 591-595

Sungucine and isosungucine are two bisindole alkaloids isolated from the roots of the African plant Strychnos icaja Baillon. They both exhibit antiplasmodial activities but also show cytotoxic effects ... [more ▼]

Sungucine and isosungucine are two bisindole alkaloids isolated from the roots of the African plant Strychnos icaja Baillon. They both exhibit antiplasmodial activities but also show cytotoxic effects against human cancer cell lines. In order to elucidate their mechanism of action, we have investigated the interaction of the alkaloids with DNA and their capacity to inhibit nucleic acids and protein synthesis in the human HL-60 promyelocytic leukemia cell line. Cell treatment with both sungucine and isosungucine leads to the appearance of a hypo-diploid DNA content peak. Western blotting analysis reveals that the two alkaloids induce cleavage of the poly(ADP-ribose) polymerase (PARP) and promote the cleavage of a caspase-3 DEVD peptide substrate. The activation of the caspase cascade is accompanied with a fragmentation of DNA in cells, as revealed by the TUNEL assay. Altogether, the results shed light on the mechanism of action of these two plant alkaloids and identify signaling factors involved in (iso)sungucine-induced apoptosis in HL-60 cells. [less ▲]

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See detailThe apoptosis of osteoblasts and osteocytes in femoral head osteonecrosis: its specificity and its distribution
Mutijima E; De Maertelaer V; Deprez M et al

in Clinical Rheumatology (2014), 33(12), 1791-5

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See detailApoptosis of Sertoli cells after conditional ablation of murine double minute 2 (Mdm2) gene is p53-dependent and results in male sterility.
Fouchecourt, S.; Livera, G.; Messiaen, S. et al

in Cell death and differentiation (2016)

Beside its well-documented role in carcinogenesis, the function of p53 family has been more recently revealed in development and female reproduction, but it is still poorly documented in male reproduction ... [more ▼]

Beside its well-documented role in carcinogenesis, the function of p53 family has been more recently revealed in development and female reproduction, but it is still poorly documented in male reproduction. We specifically tested this possibility by ablating Mdm2, an E3 ligase that regulates p53 protein stability and transactivation function, specifically in Sertoli cells (SCs) using the AMH-Cre line and created the new SC-Mdm2-/- line. Heterozygous SC-Mdm2-/+ adult males were fertile, but SC-Mdm2-/- males were infertile and exhibited: a shorter ano-genital distance, an extra duct along the vas deferens that presents a uterus-like morphology, degenerated testes with no organized seminiferous tubules and a complete loss of differentiated germ cells. In adults, testosterone levels as well as StAR, P450c17 (Cyp17a1) and P450scc (Cyp11a1) mRNA levels decreased significantly, and both plasma LH and FSH levels increased. A detailed investigation of testicular development indicated that the phenotype arose during fetal life, with SC-Mdm2-/- testes being much smaller at birth. Interestingly, Leydig cells remained present until adulthood and fetal germ cells abnormally initiated meiosis. Inactivation of Mdm2 in SCs triggered p53 activation and apoptosis as early as 15.5 days post conception with significant increase in apoptotic SCs. Importantly, testis development occurred normally in SC-Mdm2-/- lacking p53 mice (SC-Mdm2-/-p53-/-) and accordingly, these mice were fertile indicating that the aforementioned phenotypes are entirely p53-dependent. These data not only highlight the importance of keeping p53 in check for proper testicular development and male fertility but also certify the critical role of SCs in the maintenance of meiotic repression.Cell Death and Differentiation advance online publication, 16 October 2015; doi:10.1038/cdd.2015.120. [less ▲]

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See detailApoptosis-induced ectodomain shedding of hypoxia-regulated carbonic anhydrase IX from tumor cells: a double-edged response to chemotherapy.
Vidlickova, Ivana; Dequiedt, Franck ULg; Jelenska, Lenka et al

in BMC Cancer (2016), 16(1), 239

BACKGROUND: Carbonic anhydrase IX (CA IX) is a tumor-associated, highly active, transmembrane carbonic anhydrase isoform regulated by hypoxia and implicated in pH control and adhesion-migration-invasion ... [more ▼]

BACKGROUND: Carbonic anhydrase IX (CA IX) is a tumor-associated, highly active, transmembrane carbonic anhydrase isoform regulated by hypoxia and implicated in pH control and adhesion-migration-invasion. CA IX ectodomain (ECD) is shed from the tumor cell surface to serum/plasma of patients, where it can signify cancer prognosis. We previously showed that the CA IX ECD release is mediated by disintegrin and metalloproteinase ADAM17. Here we investigated the CA IX ECD shedding in tumor cells undergoing apoptosis in response to cytotoxic drugs, including cycloheximide and doxorubicin. METHODS: Presence of cell surface CA IX was correlated to the extent of apoptosis by flow cytometry in cell lines with natural or ectopic CA IX expression. CA IX ECD level was assessed by ELISA using CA IX-specific monoclonal antibodies. Effect of recombinant CA IX ECD on the activation of molecular pathways was evaluated using the cell-based dual-luciferase reporter assay. RESULTS: We found a significantly lower occurrence of apoptosis in the CA IX-positive cell subpopulation than in the CA IX-negative one. We also demonstrated that the cell-surface CA IX level dropped during the death progress due to an increased ECD shedding, which required a functional ADAM17. Inhibitors of metalloproteinases reduced CA IX ECD shedding, but not apoptosis. The CA IX ECD release induced by cytotoxic drugs was connected to elevated expression of CA IX in the surviving fraction of cells. Moreover, an externally added recombinant CA IX ECD activated a pathway driven by the Nanog transcription factor implicated in epithelial-mesenchymal transition and stemness. CONCLUSIONS: These findings imply that the increased level of the circulating CA IX ECD might be useful as an indicator of an effective antitumor chemotherapy. Conversely, elevated CA IX ECD might generate unwanted effects through autocrine/paracrine signaling potentially contributing to resistance and tumor progression. [less ▲]

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See detailL’aporie de la mathématisation de la physique en contexte aristotélicien
Dubouclez, Olivier ULg

Scientific conference (2014, February)

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See detailLes apories de l’engagement
Vrancken, Didier ULg

in Guillaume, Jean-François (Ed.) Aventuriers solitaires en quête d’utopie. Les formes contemporaines de l’engagement (2006)

Quel sens donner aujourd'hui aux notions de retrait et d'engagement sans une société de plus en plus traversée par les impératifs de participation, d'implication, de mobilisation des ressources et des ... [more ▼]

Quel sens donner aujourd'hui aux notions de retrait et d'engagement sans une société de plus en plus traversée par les impératifs de participation, d'implication, de mobilisation des ressources et des compétences ? [less ▲]

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See detailLes apories du matérialisme dialectique
Seba, Jean-Renaud ULg

Conference (2007, December 07)

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See detailApparatuses as loci of bio-political emergence: development of genetic testing in Belgium
Fallon, Catherine ULg

Conference (2015, October)

In his vision of “biopolitics” Lemke (2011) proposes to conceptualize life and politics as entities intertwined within a dynamic relationship. These technologies change the production of norms and the ... [more ▼]

In his vision of “biopolitics” Lemke (2011) proposes to conceptualize life and politics as entities intertwined within a dynamic relationship. These technologies change the production of norms and the apparatuses (Foucault 2008) organizing the distribution of the living in the domain of utility: therefore biopolitics must be analyzed as center pieces in the transformation of politics. The developments in genomics and epigenetics support the vision of the individual body as an “information network” rather than a physical substrate. The experts (with the active support of the patients) construct models for the calculation of life in the development of policy regulation and contribute actively to frame the policy relevant issues – either at individual or at population level, on the basis of power strategies mobilizing and disseminating specific forms of knowledge which are disciplined and selective. The contribution will present the recent evolution of the apparatuses producing the technical regulation and financial norms for the use of genetic testing in the federal Belgian public health system, considering the two complementary level of the “dispositif” : - At the political level, transformation of the « Conseil supérieur de la Génétique humaine » (1973-2013) into a « Collège de médecins pour le centre de génétique humaine » and the development of new control relations between the subsidizing and control bodies and the hospitals, - At the individual level, transformation of the cooperation between medical doctors, biomedical experts and psycho-sociologists in the relationship with the patients, as they can be observed at the level of the hospital; Who has the resources (intellectual, technological or financial) to control the technology and its use ? what are the social, institutional and technological constrains? who defines and select the legitimate options ? The analysis is not so much normative than critical, giving due attention to the social context and contingent rationalities in the evolution of this peculiar political action, with the aim of opening new areas of discussions transgressing disciplinary and institutional borders which are too often considered as self evident. [less ▲]

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See detailL'appareil de saut des Taupins (Coléoptères Elatérides)
Jeuniaux, Charles ULg

Article for general public (1950)

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See detailL’appareil sexuel femelle de l’Ascaride mégalocéphale
Van Beneden, Édouard ULg

in Archives de Biologie (1883), 4

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See detailL'appareil vocal
FINCK, Camille ULg

in Rossius, Raymond (Ed.) Le monde de l'Opéra (1997)

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See detailAppareillage informatique et logiciels d'analyse qualitative en sciences humaines et sociales : réflexions épistémologiques et méthodologiques
Lejeune, Christophe ULg

Scientific conference (2008, September 26)

L'histoire des logiciels d'analyse qualitative en sciences débute dans les années soixante. Il faut cependant attendre les années quatre-vingt et l'avènement de la micro-informatique pour voir se ... [more ▼]

L'histoire des logiciels d'analyse qualitative en sciences débute dans les années soixante. Il faut cependant attendre les années quatre-vingt et l'avènement de la micro-informatique pour voir se multiplier ces outils. On peut regrouper les dizaines d'outils existant à ce jour en quatre familles selon que les traitements soient (ou non) automatisés et que le logiciel prend en charge l'exploration ou l'annotation du matériau : les lexicomètres, les concordanciers, les automates et les CAQDAS. Bien entendu, les logiciels existant proposent chacun un grand nombre de fonctionnalités (susceptibles de relever de différentes familles); notre distinction porte donc sur les fonctionnalités plus que sur les outils. Chacune de ces familles relève d'une tradition que remonte à bien avant l'ère de l'informatique (les concordanciers, par exemple, datent du XIIIe siècle). Celle-ci a participé à forger une technique congruente avec ses fins et son matériau. En explicitant la configuration historico-technique des différents logiciels proposés aujourd'hui au qualitativiste, on se rend compte que - contrairement à ce qui est professé et pratiqué - les différentes fonctionnalités s'appuient sur des présupposés épistémologiques plus ou moins pertinents selon le matériaux et la question de recherche. En définitive, on voudrait inviter les chercheurs en sciences humaines et sociales à une réflexion méthodologique et épistémologique sur l'appareillage informatique constitutif de leur dispositif méthodologique. [less ▲]

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See detailL’appareillage orthétique distal du membre inférieur chez le patient neurologique
DOUCHAMPS, Frédéric ULg; Kaux, Jean-François ULg; WANG, François-Charles ULg

in Lettre du Neurologue (La) : le Courrier du Spécialiste (2012), XVI(8), 268-274

La prescription d’une orthèse doit tenir compte: • de la spasticité des muscles tibial postérieur et triceps sural (échelle d’Ashworth) ; • de la force des releveurs (échelle de Lovett) ; • de la statique ... [more ▼]

La prescription d’une orthèse doit tenir compte: • de la spasticité des muscles tibial postérieur et triceps sural (échelle d’Ashworth) ; • de la force des releveurs (échelle de Lovett) ; • de la statique plantaire (pied varus et/ou talus en phase d’appui) ; • de l’âge et du niveau d’activité (sédentaire, sportif) ; • de l’attente des patients. [less ▲]

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