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See detailIdentification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytiea
Fickers, Patrick ULg; Fudalej, F.; Le Dall, M. T. et al

in Fungal Genetics and Biology (2005), 42(3), 264-274

In the lipolytic yeast Yarrowia lipolytica, the LIP2 gene was previously reported to encode all extracellular lipase. The growth of a Deltalip2 strain on triglycerides as sole carbon source suggest all ... [more ▼]

In the lipolytic yeast Yarrowia lipolytica, the LIP2 gene was previously reported to encode all extracellular lipase. The growth of a Deltalip2 strain on triglycerides as sole carbon source suggest all alternative pathway for triglycerides utilisation ill this yeast. Here, we describe the isolation and the characterisation of the LIP7 and LIP8 genes which were found to encode a 366 and a 371-amino acid precursor protein, respectively. These proteins which belong to the triacylglycerol hydrolase family (EC 3.1.1.3) presented a high homology with the extracellular lipase CdLIP2 and CdLIP3 from Candida deformans. The physiological function of the lipase isoenzymes was investigated by creating single and multi-disrupted strains. Lip7p and Lip8p were found to correspond to active secreted lipases. The lack of lipase production in a Deltalip2 Deltalip7 Deltalip8 strain suggest that no additional extracellular lipase remains to be discovered in Y. lipolytica. The substrate specificity towards synthetic ester molecules indicates that Lip7p presented a maximum activity centred on caproate (C-6) while that of Lip8p is in caprate (C-10). (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

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See detailIdentification and characterisation of Phaseolus vulgaris L. EMS mutants failing in seed development
Silué, S.; Lariguet, P.; Pankhurst, C. et al

Conference given outside the academic context (2007)

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See detailIdentification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota
Martin, Marjolaine ULg; Biver, Sophie ULg; Steels, Sébastien ULg et al

in Applied and Environmental Microbiology (2014), 80(16), 4958-4967

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two ... [more ▼]

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions. [less ▲]

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See detailIdentification and characterization of a protozoan uncoupling protein in Acanthamoeba castellanii.
Jarmuszkiewicz, W.; Sluse-goffart, C.; Hryniewiecka, L. et al

in Journal of Biological Chemistry (1999), 274(33), 23198-23202

An uncoupling protein (UCP) has been identified in mitochondria from Acanthamoeba castellanii, a nonphotosynthetic soil amoeboid protozoon that, in molecular phylogenesis, appears on a branch basal to the ... [more ▼]

An uncoupling protein (UCP) has been identified in mitochondria from Acanthamoeba castellanii, a nonphotosynthetic soil amoeboid protozoon that, in molecular phylogenesis, appears on a branch basal to the divergence points of plants, animals, and fungi. The existence of UCP in A. castellanii (AcUCP) has been revealed using antibodies raised against plant UCP. Its molecular mass (32,000 Da) was similar to those of plant and mammalian UCPs. The activity of AcUCP has been investigated in mitochondria depleted of free fatty acids. Additions of linoleic acid stimulated state 4 respiration and decreased transmembrane electrical potential (DeltaPsi) in a manner expected from fatty acid cycling-linked H(+) reuptake. The half-maximal stimulation by linoleic acid was reached at 8.1 +/- 0.4 microM. Bovine serum albumin (fatty acid-free), which adsorbs linoleic acid, reversed the respiratory stimulation and correspondingly restored DeltaPsi. AcUCP was only weakly inhibited by purine nucleotides like UCP in plants. A single force-flow relationship has been observed for state 4 respiration with increasing concentration of linoleic acid or of an uncoupler and for state 3 respiration with increasing concentration of oligomycin, indicating that linoleic acid has a pure protonophoric effect. The activity of AcUCP in state 3 has been evidenced by ADP/oxygen atom determination. The discovery of AcUCP indicates that UCPs emerged, as specialized proteins for H(+) cycling, early during phylogenesis before the major radiation of phenotypic diversity in eukaryotes and could occur in the whole eukaryotic world. [less ▲]

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See detailIdentification and characterization of a protozoan uncoupling protein in Acanthamoeba castellanii.
Jarmuszkiwicz, W.; Milani, G.; Fortes, F. et al

in FEBS Letters (2000), 467

An uncoupling protein (UCP) was identified in mitochondria from Candida parapsilosis (CpUCP), a non-fermentative parasitic yeast. CpUCP was immunodetected using polyclonal antibodies raised against plant ... [more ▼]

An uncoupling protein (UCP) was identified in mitochondria from Candida parapsilosis (CpUCP), a non-fermentative parasitic yeast. CpUCP was immunodetected using polyclonal antibodies raised against plant UCP. Activity of CpUCP, investigated in mitochondria depleted of free fatty acids, was stimulated by linoleic acid (LA) and inhibited by GTP. Activity of CpUCP enhanced state 4 respiration by decreasing DeltaPsi and lowered the ADP/O ratio. Thus, it was able to divert energy from oxidative phosphorylation. The voltage dependence of electron flux indicated that LA had a pure protonophoretic effect. The discovery of CpUCP proves that UCP-like proteins occur in the four eukaryotic kingdoms: animals, plants, fungi and protists. [less ▲]

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See detailIdentification and characterization of angiotensin-II receptor subtypes in cultured bovine and human adrenal fasciculata cells and PC12W cells.
Ouali, R.; LEBRETHON, Marie-Christine ULg; Saez, J. M.

in Endocrinology (1993), 133(6), 2766-72

Angiotensin-II (Ang II) receptor subtypes (AT1 and AT2) were analyzed in bovine adrenal cells (BAC) by binding and cross-linking experiments using [125I]Ang II and [125I]CGP42112, a specific ligand of AT2 ... [more ▼]

Angiotensin-II (Ang II) receptor subtypes (AT1 and AT2) were analyzed in bovine adrenal cells (BAC) by binding and cross-linking experiments using [125I]Ang II and [125I]CGP42112, a specific ligand of AT2 receptors. [125I]Ang II binding was reduced by 80% and 20% in the presence of maximal concentrations of the AT1 antagonist losartan (DuP 753) and CGP42112, respectively, whereas [125I]CGP42112 binding was inhibited by Ang II or CGP42112, but not by losartan. In the presence of the reducing agent dithio-1,4-erythritol, the binding of [125I] CGP42112 was increased 2-fold; this was due to an increase in the binding affinity (Kd, 8 +/- 4 x 10(-10) vs. 4.8 +/- 1.2 x 10(-10) M). Cross-linking of [125I]Ang II to BAC in the presence of disuccinimidyl suberate, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealed a band of 70,000 +/- 8,000 mol wt (M(r)) under both reducing and nonreducing conditions. This band disappeared when the incubation was performed in the presence of 10(-6) M Ang II or 5 x 10(-8) M CGP42112, but not in the presence of 10(-5) M losartan. Dithio-1,4-erythritol (10 mM) markedly enhanced the band. After cross-linking with 1,5-difluoro-2,4-dinitrobenzene and solubilization of the cells in the presence of protease inhibitors, two radioactive bands were observed with M(r) of 70,000 and 50,000. The first disappeared after the addition of Ang II or CGP42112, whereas the second disappeared in the presence of Ang II or losartan, but not in the presence of CGP42112. Cross-linking of [125I]AngII to either human adrenal fasciculata-reticularis cells, which contain only AT1 sites, or COS-7 cells transfected with human AT1 cDNA revealed a major band of 50,000 M(r) that was blunted by Ang II or losartan, but not by CGP42112. Moreover, cross-linking of [125I]Ang II to PC12W cells, which contain only the AT2 receptor subtype, revealed a single radioactive band of 70,000 Mr that was blunted by CGP42112 but not by losartan. Thus, in both BAC and PC12W cells, the AT2 receptor has a M(r) of 70,000, whereas the AT1 receptor in BAC, human adrenal cells, and cells transfected with human AT1 receptor cDNA has a Mr of 50,000. Therefore, the heterogeneity of the size of the Ang II receptor previously reported after photoaffinity or cross-linking was probably due to only to a variation in the degree of glycosylation between tissues and species, but also to the presence of two different receptor subtypes. [less ▲]

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See detailIdentification and Characterization of Bovine Herpesvirus Type 5 Glycoprotein H Gene and Gene Products
Meyer, Gilles; Bare, O.; Thiry, Etienne ULg

in Journal of General Virology (The) (1999), 80(Pt 11), 2849-59

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 ... [more ▼]

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 glycoprotein gH was sequenced. A high degree of conservation was found between BHV-1 and BHV-5 deduced gH amino acid sequences (86. 4%), which is also observed for all alphaherpesvirus gH sequences. Transcriptional analysis revealed a 3.1 kb mRNA as the specific gH transcript which was detected 2 h post-infection (p.i.). Twelve out of twenty-one MAbs directed against BHV-1 gH immunoprecipitated a 108-110 kDa glycoprotein, which was then designated BHV-5 gH. Synthesis and intracellular processing of BHV- 5 gH was analysed in infected MDBK cells using gH cross-reacting MAbs. Glycoprotein gH was expressed as a beta-gamma protein, detected by radioimmunoprecipitation as early as 3 h p.i. Glycosylation studies indicated that BHV-5 gH contains N-linked carbohydrates which are essential for the recognition of the protein by the MAbs. This suggests that N-linked glycans are involved in protein folding or are targets for the gH cross-reacting MAbs. Plaque- reduction neutralization assays showed that at least one BHV-1 gH antigenic domain is lacking in BHV-5 which may possibly relate to in vivo differences in virus tropism. [less ▲]

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See detailIdentification and characterization of drought stress responsive genes in faba bean (Vicia faba L.) by suppression subtractive hybridization
Abid, Ghassen; Muhovski, Yordan; Mingeot, Dominique et al

in Plant Cell, Tissue & Organ Culture (2014)

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See detailIdentification and characterization of four splicing variants of ovine POUF1 gene
Bastos, Estella; Avila, S.; Cravador, Alfredo et al

in Gene (2006), 382

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See detailIdentification and Characterization of Glycoprotein Gp1 of Bovine Herpesvirus Type 4
Dubuisson, J.; Pastoret, Paul-Pierre ULg; Thiry, Etienne ULg

in Journal of General Virology (The) (1992), 73((Pt 5)), 1293-6

Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens had ... [more ▼]

Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens had been removed by immunoaffinity, a fourth glycoprotein was identified. This protein (gp1) has a high Mr (greater than 300K), is detected about 8 h post-inoculation of infected cells and is strictly expressed as a gamma protein. Moreover, gp1 was identified by a polyclonal antiserum from an infected animal, indicating that this glycoprotein is an antigen recognized by the immune system of infected animals. [less ▲]

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See detailIdentification and characterization of in vivo attenuated mutants of Brucella melitensis.
Lestrate, P.; Delrue, R. M.; DANESE, Isabelle ULg et al

in Molecular Microbiology (2000), 38(3), 543-51

Brucella melitensis 16M is a Gram-negative alpha2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within ... [more ▼]

Brucella melitensis 16M is a Gram-negative alpha2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. Signature-tagged mutagenesis (STM) was used to identify genes required for the in vivo pathogenesis of Brucella. A library of transposon mutants was screened in a murine infection model. Out of 672 mutants screened, 20 were not recovered after a 5 day passage in BALB/c mice. The attenuation of 18 mutants was confirmed using an in vivo competition assay against the wild-type strain. The 18 mutants were characterized further for their ability to replicate in murine macrophages and in HeLa cells. The sequences disrupted by the transposon in the mutants have homology to genes coding for proteins of different functional classes: transport, amino acid and DNA metabolism, transcriptional regulation, peptidoglycan synthesis, a chaperone-like protein and proteins of unknown function. The mutants selected in this study provide new insights into the molecular basis of Brucella virulence. [less ▲]

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See detailIdentification and characterization of mRNAs differentially expressed in thyroid cells stimulated by a mitogenic treatment
Pirson, I.; Behrens, J.; Savonet, V. et al

in Biochimie (1999), 81

he aim of our work is to identify new genes and proteins involved in the control of the proliferation of thyroid cells as putative protooncogenes and antioncogenes. Several strategies are discussed. A ... [more ▼]

he aim of our work is to identify new genes and proteins involved in the control of the proliferation of thyroid cells as putative protooncogenes and antioncogenes. Several strategies are discussed. A first study has allowed to identify three new genes. Further search will use the differential display and gene arrays methodology. The role of the identified proteins coded by the genes is studied in vitro by the search of partner proteins by the double hybrid method and in vivo by mice gene knockout technology [less ▲]

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See detailIdentification and characterization of new blood-accessible colorectal cancer biomarkers
Conrotto, Paolo; Roesli, Christoph; Rybak, J. et al

Conference (2008)

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See detailIdentification and characterization of novel bovine leukemia virus (BLV) antisense transcripts in leukemic and pre-leukemic clones
Durkin, Keith ULg; Rosewick, Nicolas; Artesi, Maria ULg et al

Conference (2016, May 21)

The deltaretrovirus Bovine Leukemia Virus (BLV) is closely related to the Human T-cell leukemia virus-1 (HTLV-1). Cattle are the natural host of BLV where it integrates into B-cells, produces a lifelong ... [more ▼]

The deltaretrovirus Bovine Leukemia Virus (BLV) is closely related to the Human T-cell leukemia virus-1 (HTLV-1). Cattle are the natural host of BLV where it integrates into B-cells, produces a lifelong infection. Most infected animals remain asymptomatic but following a protracted latency period about ~5% develop an aggressive leukemia/lymphoma, mirroring the disease trajectory of HTLV-1. Like the case in HTLV-1 the 5’LTR BLV provirus is transcriptionally silent in tumors, however the provirus is not entirely quiescent, constitutively express the BLV microRNAs in tumors. Using RNA-seq, we found that in addition to microRNAs, the BLV provirus also constitutively expresses two antisense transcripts in all BLV infected samples examined. The first transcript (AS1) has alternate potential polyadenylation sites generating a short transcript of ~600bp (AS1-S) and a less abundant longer transcript of ~2200bp (AS1-L). Alternative splicing also creates a second transcript of ~400bp (AS2) utilizing the first exon of AS1. Production of AS transcripts from the 3’LTR was supported by reporter assays demonstrating that the BLV LTR has substantial and Tax-independent antisense promoter activity. BLV AS transcripts predominantly localize in the nucleus. Examination of protein coding potential showed AS2 to be non-coding, while the AS1-S/L transcripts coding potential is ambiguous, with a small potential open reading frame (ORF) of 264bp present. The AS1-L transcript overlaps the BLV microRNAs transcribed in the sense direction. Using high throughput sequencing of RNA-ligase-mediated (RLM) 5' RACE products, we show that the perfect complementary between the transcripts leads to RNA-induced silencing complex (RISC) mediated cleavage of AS1-L. Furthermore, experiments using BLV proviruses where the microRNAs were removed or inverted point to additional transcriptional interactions between the two viral RNA species. Knock down of AS1-S/L using locked nucleic acids (LNAs) showed no obvious effect on the cells phenotype. While a detailed elucidation of the BLV antisense transcripts function remains in the future, the constitutive expression in all samples examined, points to a vital role for the transcripts in the life cycle and oncogenic potential of BLV. [less ▲]

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See detailIdentification and characterization of novel galectin-9 splice variants in endothelial cells
Heusschen, Roy ULg; De Bree, Martijn; Griffioen, Arjan et al

in Cancer Research (2011)

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See detailIdentification and characterization of novel galectin-9 splice variants in endothelial cells
Heusschen, Roy ULg; De Bree, Martijn; Griffioen, Arjan et al

Poster (2011)

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See detailIdentification and characterization of novel peptidoglycan glycosyltransferase inhibitors with antibacterial activity
Derouaux, Adeline ULg; Turk, Samo; Offant, Julien et al

Poster (2009, November)

Detailed reference viewed: 29 (3 ULg)