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See detail"Identical" reconstruction and Heritage Authenticity: introduction to the session
Dawans, Stéphane ULg; Houbart, Claudine ULg; Piplani, Navin

Conference (2012, June 06)

Introduction to the session ""Identical" Reconstruction and Heritage Authenticity", highlighting the most prominent issues of the current debate in an international context.

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See detailAs identidades confrontadas ao ensino de línguas e culturas estrangeiras
Defays, Jean-Marc ULg

Conference (2014, October 15)

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See detailIdentidades en Bélgica
Jacquemain, Marc ULg

Scientific conference (2011, July 14)

Una historia paralela del movimiento flamenco y del movimiento walon desde 1815 para explicar a ensenantes y estudaintes peruanos la dialectica economica/cultural del antagonismo entre comunidades en ... [more ▼]

Una historia paralela del movimiento flamenco y del movimiento walon desde 1815 para explicar a ensenantes y estudaintes peruanos la dialectica economica/cultural del antagonismo entre comunidades en Belgica hasta la paralisis actual del gobierno federal y el posible estallamieto del pais. [less ▲]

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See detailIdentificación de adhesinas F17 y CS31A en Escherichia coli aislados de terneros diarreicos o septicémicos.
Mercado, E.C.; Rodriguez, Sabrina ULg; Elizondo, A.

Poster (2002, November 12)

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See detailIdentificación de parámetros de viscoelasticidad finita aplicada a la simulación del comportamiento mecánico de masa encefálica
Fancello, Eduardo; Vigneron, Lara; Noels, Ludovic ULg et al

in Congreso Iberoamericano de Ingenieria Mecanica (2007, October)

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See detailIdentificación de parásitos intestinales de primates en dos centros de rescate en la amazonía ecuatoriana
Martin, Sarah ULg; Carrillo Bilbao, Gabriel Alberto; Celi, Maritza et al

in Tirira, Diego (Ed.) Memorias XXXv Jornadas Nacional de Biología, Quito, 17-19 Noviembre 2011, I Congreso Ecuatoriano de Mastozoología (2011, November 17)

Los primates son reservorios de los patógenos humanos. Si identificamos las enfermedades parasitarias de los primates, puede ser favorable para su conservación. Examinamos las muestras fecales para ... [more ▼]

Los primates son reservorios de los patógenos humanos. Si identificamos las enfermedades parasitarias de los primates, puede ser favorable para su conservación. Examinamos las muestras fecales para identificar los parásitos intestinales que se encuentran en las especies de primates en dos centros de rescate en la amazonía ecuatoriana. Estas muestras se analizaron según varios factores como tamaño del grupo, el sexo y la edad. La prevalencia general de protozoarios y de helmintos que se encontraron en las muestras fue de 17.6 % y de 55.4 % respectivamente. Más de la mitad de los parásitos encontrados en este estudio (Necator/Ancylostoma (6.8%), Capillaria sp. (4%), Strongylus sp. (41.9%), Entamoeba histolytica (13.5%)) son una amenaza potencial de transmisión zoonótica. Estos resultados preliminares muestran una diversidad importante de parásitos zoonóticos en los primates en cautiverio y resultados posteriores podrían demostrar la importancia de estos en la salud pública y la conservación de primates. [less ▲]

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See detailIdentificación y caracterización de un nuevo elemento regulatorio que involucra al factor transcripcional E2F4 en la región U5 del LTR del virus de la leucosis bovina
Rodriguez, Sabrina ULg; Varone, C.; Cánepa, E. et al

Poster (2003, December 01)

IDENTIFICACIÓN Y CARACTERIZACIÓN DE UN NUEVO ELEMENTO REGULATORIO QUE INVOLUCRA AL FACTOR TRANSCRIPCIONAL E2F-4 EN LA REGIÓN U5 DEL LTR DEL VIRUS DE LA LEUCOSIS BOVINA S. Rodriguez1, C. Varone2, E ... [more ▼]

IDENTIFICACIÓN Y CARACTERIZACIÓN DE UN NUEVO ELEMENTO REGULATORIO QUE INVOLUCRA AL FACTOR TRANSCRIPCIONAL E2F-4 EN LA REGIÓN U5 DEL LTR DEL VIRUS DE LA LEUCOSIS BOVINA S. Rodriguez1, C. Varone2, E. Cánepa2, K. Trono1 1Instituto de Virología, CICVyA. INTA – Castelar. 2Cátedra de Biologia Molecular, Facultad de Ciencias Exactas, UBA. Buenos Aires, Argentina. El genoma del virus de la leucosis bovina (VLB), como todo retrovirus, presenta en ambos extremos zonas denominadas Long Terminal Repeats (LTRs) de importancia en la regulación de la transcripción. Estas regiones están organizadas en tres zonas llamadas U3, R y U5. La región U3 ha sido ampliamente estudiada y se sabe que contiene los principales sitios que regulan la transcripción viral. Sin embargo, poco se sabe de las regiones R y U5 aunque ha quedado demostrado que ambas zonas contienen asimismo secuencias estimulatorias. Con el objetivo de identificar y caracterizar nuevos elementos regulatorios, en este trabajo se ha llevado a cabo el estudio de secuencias de las regiones R y U5 del LTR 5´ de BLV. El análisis de patrones de reconocimiento de secuencias consenso para factores de transcripción celulares en estas regiones, reveló la presencia de 2 posibles elementos de unión para E2F, ambos en la zona U5, denominados E2F-A y E2F-B, respectivamente. Para analizar la capacidad de unión a factores celulares de las secuencias LTRs se llevaron a cabo ensayos de cambio en la movilidad electroforética (EMSA) y supershift utilizando extractos celulares obtenidos a partir de linfocitos bovinos y empleando los oligonucleótidos U51 y U52 como sondas, donde mapean los elementos E2FA y E2FB, respectivamente. Se observó retardo por la formación de complejos ribo-proteicos en ambos casos pero solo en el caso del complejo U51-E2F pudo ser identificado el factor E2F-4 utilizando anticuerpos específicos. En resumen, estos resultados permiten identificar un nuevo sitio para el factor de transcripción E2F-4 localizado en la región U5 del LTR 5´ de BLV. Este motivo constituye un nuevo elemento regulatorio involucrado en la actividad transcripcional de genes virales y celulares dirigida por los LTRs y podría jugar un rol importante en la replicación viral. [less ▲]

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See detailIdentification and Analysis of Differentially Expressed Genes During Seed Development Using Suppression Subtractive Hybridization (SSH) in Phaseolus vulgaris
Abid, Ghassen ULg; Sassi, Khaled; Muhovski, Yordan et al

in Plant Molecular Biology Reporter (2011)

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See detailIdentification and biochemical characteristics of lipopeptides from Bacillus mojavensis A21
Ben Ayed, Hanen; Hmidet, Noomen; Béchet, Max et al

in Process Biochemistry (2014), 49(10), 1699-1707

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See detailIdentification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytiea
Fickers, Patrick ULg; Fudalej, F.; Le Dall, M. T. et al

in Fungal Genetics and Biology (2005), 42(3), 264-274

In the lipolytic yeast Yarrowia lipolytica, the LIP2 gene was previously reported to encode all extracellular lipase. The growth of a Deltalip2 strain on triglycerides as sole carbon source suggest all ... [more ▼]

In the lipolytic yeast Yarrowia lipolytica, the LIP2 gene was previously reported to encode all extracellular lipase. The growth of a Deltalip2 strain on triglycerides as sole carbon source suggest all alternative pathway for triglycerides utilisation ill this yeast. Here, we describe the isolation and the characterisation of the LIP7 and LIP8 genes which were found to encode a 366 and a 371-amino acid precursor protein, respectively. These proteins which belong to the triacylglycerol hydrolase family (EC 3.1.1.3) presented a high homology with the extracellular lipase CdLIP2 and CdLIP3 from Candida deformans. The physiological function of the lipase isoenzymes was investigated by creating single and multi-disrupted strains. Lip7p and Lip8p were found to correspond to active secreted lipases. The lack of lipase production in a Deltalip2 Deltalip7 Deltalip8 strain suggest that no additional extracellular lipase remains to be discovered in Y. lipolytica. The substrate specificity towards synthetic ester molecules indicates that Lip7p presented a maximum activity centred on caproate (C-6) while that of Lip8p is in caprate (C-10). (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

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See detailIdentification and characterisation of Phaseolus vulgaris L. EMS mutants failing in seed development
Silué, S.; Lariguet, P.; Pankhurst, C. et al

Conference given outside the academic context (2007)

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See detailIdentification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota
Martin, Marjolaine ULg; Biver, Sophie ULg; Steels, Sébastien ULg et al

in Applied and Environmental Microbiology (2014), 80(16), 4958-4967

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two ... [more ▼]

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions. [less ▲]

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See detailIdentification and characterization of a protozoan uncoupling protein in Acanthamoeba castellanii.
Jarmuszkiewicz, W.; Sluse-goffart, C.; Hryniewiecka, L. et al

in Journal of Biological Chemistry (1999), 274(33), 23198-23202

An uncoupling protein (UCP) has been identified in mitochondria from Acanthamoeba castellanii, a nonphotosynthetic soil amoeboid protozoon that, in molecular phylogenesis, appears on a branch basal to the ... [more ▼]

An uncoupling protein (UCP) has been identified in mitochondria from Acanthamoeba castellanii, a nonphotosynthetic soil amoeboid protozoon that, in molecular phylogenesis, appears on a branch basal to the divergence points of plants, animals, and fungi. The existence of UCP in A. castellanii (AcUCP) has been revealed using antibodies raised against plant UCP. Its molecular mass (32,000 Da) was similar to those of plant and mammalian UCPs. The activity of AcUCP has been investigated in mitochondria depleted of free fatty acids. Additions of linoleic acid stimulated state 4 respiration and decreased transmembrane electrical potential (DeltaPsi) in a manner expected from fatty acid cycling-linked H(+) reuptake. The half-maximal stimulation by linoleic acid was reached at 8.1 +/- 0.4 microM. Bovine serum albumin (fatty acid-free), which adsorbs linoleic acid, reversed the respiratory stimulation and correspondingly restored DeltaPsi. AcUCP was only weakly inhibited by purine nucleotides like UCP in plants. A single force-flow relationship has been observed for state 4 respiration with increasing concentration of linoleic acid or of an uncoupler and for state 3 respiration with increasing concentration of oligomycin, indicating that linoleic acid has a pure protonophoric effect. The activity of AcUCP in state 3 has been evidenced by ADP/oxygen atom determination. The discovery of AcUCP indicates that UCPs emerged, as specialized proteins for H(+) cycling, early during phylogenesis before the major radiation of phenotypic diversity in eukaryotes and could occur in the whole eukaryotic world. [less ▲]

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See detailIdentification and characterization of a protozoan uncoupling protein in Acanthamoeba castellanii.
Jarmuszkiwicz, W.; Milani, G.; Fortes, F. et al

in FEBS Letters (2000), 467

An uncoupling protein (UCP) was identified in mitochondria from Candida parapsilosis (CpUCP), a non-fermentative parasitic yeast. CpUCP was immunodetected using polyclonal antibodies raised against plant ... [more ▼]

An uncoupling protein (UCP) was identified in mitochondria from Candida parapsilosis (CpUCP), a non-fermentative parasitic yeast. CpUCP was immunodetected using polyclonal antibodies raised against plant UCP. Activity of CpUCP, investigated in mitochondria depleted of free fatty acids, was stimulated by linoleic acid (LA) and inhibited by GTP. Activity of CpUCP enhanced state 4 respiration by decreasing DeltaPsi and lowered the ADP/O ratio. Thus, it was able to divert energy from oxidative phosphorylation. The voltage dependence of electron flux indicated that LA had a pure protonophoretic effect. The discovery of CpUCP proves that UCP-like proteins occur in the four eukaryotic kingdoms: animals, plants, fungi and protists. [less ▲]

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See detailIdentification and characterization of angiotensin-II receptor subtypes in cultured bovine and human adrenal fasciculata cells and PC12W cells.
Ouali, R.; LEBRETHON, Marie-Christine ULg; Saez, J. M.

in Endocrinology (1993), 133(6), 2766-72

Angiotensin-II (Ang II) receptor subtypes (AT1 and AT2) were analyzed in bovine adrenal cells (BAC) by binding and cross-linking experiments using [125I]Ang II and [125I]CGP42112, a specific ligand of AT2 ... [more ▼]

Angiotensin-II (Ang II) receptor subtypes (AT1 and AT2) were analyzed in bovine adrenal cells (BAC) by binding and cross-linking experiments using [125I]Ang II and [125I]CGP42112, a specific ligand of AT2 receptors. [125I]Ang II binding was reduced by 80% and 20% in the presence of maximal concentrations of the AT1 antagonist losartan (DuP 753) and CGP42112, respectively, whereas [125I]CGP42112 binding was inhibited by Ang II or CGP42112, but not by losartan. In the presence of the reducing agent dithio-1,4-erythritol, the binding of [125I] CGP42112 was increased 2-fold; this was due to an increase in the binding affinity (Kd, 8 +/- 4 x 10(-10) vs. 4.8 +/- 1.2 x 10(-10) M). Cross-linking of [125I]Ang II to BAC in the presence of disuccinimidyl suberate, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealed a band of 70,000 +/- 8,000 mol wt (M(r)) under both reducing and nonreducing conditions. This band disappeared when the incubation was performed in the presence of 10(-6) M Ang II or 5 x 10(-8) M CGP42112, but not in the presence of 10(-5) M losartan. Dithio-1,4-erythritol (10 mM) markedly enhanced the band. After cross-linking with 1,5-difluoro-2,4-dinitrobenzene and solubilization of the cells in the presence of protease inhibitors, two radioactive bands were observed with M(r) of 70,000 and 50,000. The first disappeared after the addition of Ang II or CGP42112, whereas the second disappeared in the presence of Ang II or losartan, but not in the presence of CGP42112. Cross-linking of [125I]AngII to either human adrenal fasciculata-reticularis cells, which contain only AT1 sites, or COS-7 cells transfected with human AT1 cDNA revealed a major band of 50,000 M(r) that was blunted by Ang II or losartan, but not by CGP42112. Moreover, cross-linking of [125I]Ang II to PC12W cells, which contain only the AT2 receptor subtype, revealed a single radioactive band of 70,000 Mr that was blunted by CGP42112 but not by losartan. Thus, in both BAC and PC12W cells, the AT2 receptor has a M(r) of 70,000, whereas the AT1 receptor in BAC, human adrenal cells, and cells transfected with human AT1 receptor cDNA has a Mr of 50,000. Therefore, the heterogeneity of the size of the Ang II receptor previously reported after photoaffinity or cross-linking was probably due to only to a variation in the degree of glycosylation between tissues and species, but also to the presence of two different receptor subtypes. [less ▲]

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