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See detailLow dentin matrix protein 1 expression correlates with skeletal metastases development in breast cancer patients and enhances cell migratory capacity in vitro
Bucciarelli, E.; Sidoni, A.; Bellezza, G. et al

in Breast Cancer Research and Treatment (2007), 105(1), 95-104

Small integrin-binding ligand N-linked glycoproteins (SIBLINGs) constitute a family of extracellular matrix proteins involved in bone homeostasis. Their pattern of expression has been primarily reported ... [more ▼]

Small integrin-binding ligand N-linked glycoproteins (SIBLINGs) constitute a family of extracellular matrix proteins involved in bone homeostasis. Their pattern of expression has been primarily reported in bone and tooth and, more recently, in several cancer types. Dentin matrix protein 1 (DMP1), a SIBLING family member, expression was investigated by immunohistochemistry in a retrospective series of 148 primary human breast cancers. Correlations between DMP1 expression levels in the tumors and clinicopathologic features, bone metastases development and relapse of the disease were examined. DMP1 was expressed by 63.5% of the breast tumors analyzed. Significant inverse associations were found between DMP1 expression levels and the size and grade of the tumors (both, P < 0.0001). High DMP1 expression levels in the primary breast lesions were associated with a lower risk of subsequent development of skeletal metastases (P = 0.009). Patients with tumors expressing high levels of DMP1 had a significantly higher disease-free survival rate than those with low DMP1-expressing tumors (P = 0.0062). When DMP1 expression was examined in breast cancer cell lines, we found that non invasive MCF-7 and T47-D cells expressed higher levels than highly invasive MDA-MB-231 and Hs578T cells. Moreover, the specific inhibition of DMP1 expression in MCF-7 cells using siRNAs promoted significantly their migratory capability. Our data implicate for the first time DMP1 expression in breast cancer progression and bone metastases development. [less ▲]

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See detailLow detection HPLC – DAD analysis of aldehydes and ketones in indoor air : Development and validation by accuracy profile
Marlet, Christelle ULg; Haesen, Charlotte ULg; Lognay, Georges ULg

Poster (2010, January)

Aliphatic aldehydes and ketones like formaldehyde are reactive volatile compounds and common indoor pollutants. The main sources are tobacco smoke, combustion gases, painting and materials. Theses ... [more ▼]

Aliphatic aldehydes and ketones like formaldehyde are reactive volatile compounds and common indoor pollutants. The main sources are tobacco smoke, combustion gases, painting and materials. Theses compounds also arise from the reaction between unsaturated compounds and oxidative agents. The toxic potential of formaldehyde and other aliphatic/aromatic carbonyl compounds requires the determination of even low amounts of these compounds in indoor air. The present method for indoor monitoring uses adsorption at 2,4-dinitrophenylhydrazine (DNPH)-coated sorbents followed by HPLC-UV/DAD analysis of the extract. Detection limits of 0.1μg/m3 were reached by this method which is sufficient for indoor air monitoring. Three DNPH-derivatives could be separated well on a RP-Amide column followed by UV/DAD-detection at 360 nm. Recovery rates of 70-100 % were determined using liquid spiking method. Validation strategy was intentionally oriented towards the ease of use in routine and the liability of the method rather than extreme performances. For that, the validation by accuracy profile was chosen. The accuracy profile, based on the concept of total error (bias + standard deviation), allows to correctly conclude about the validity of a procedure and thus, guarantees that a known proportion of future results obtained with the method will be included within acceptance limits. For the three tested compounds, formaldehyde, acetaldehyde and acetone, the accuracy profile gave the guarantee that at least 95% of the future results obtained with the validated method will be within the ±25% acceptance limits over the whole concentration range defined. The HPLC parameters were also determinated. The performance of the method was finally evaluated by the analysis of indoor air from new timber frame construction. [less ▲]

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See detailLow dietary calcium intake in patients treated for osteoporosis
Reginster, Jean-Yves ULg; Imschoot, J.

in Osteoporosis International (2007, March), 18(Suppl.1), 132

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See detailLow dietary intake calcium in European postmenopausal osteoporotic women
Bruyère, Olivier ULg; DeCock, Caroline; Mottet, Catherine et al

in Public Health Nutrition (2009), 12(1), 111-114

Objective The WHO recommends a daily Ca intake for postmenopausal women of 1300 mg. The objective of the present study was to assess the dietary Ca intake in European postmenopausal osteoporotic women ... [more ▼]

Objective The WHO recommends a daily Ca intake for postmenopausal women of 1300 mg. The objective of the present study was to assess the dietary Ca intake in European postmenopausal osteoporotic women. Design, setting and subjects Assessment of dietary Ca intake (food and supplements) was performed with a validated self-questionnaire in 8524 osteoporotic women from nine European countries (Belgium, Denmark, France, Germany, Hungary, Italy, Poland, Spain and the UK). Results Mean age of the patients was 74·2 (sd 7·1) years, mean BMI was 25·7 (sd 4·2) kg/m2. Of the study population, 37·2 % of the women took Ca supplements. The mean dietary intake of Ca was 930·7 (sd 422·9) mg/d. The lowest Ca intake was found in Hungary (586·7 (sd 319·1) mg/d) and the highest in Denmark (1145·6 (sd 463·0) mg/d). In the whole study population, only 19·1 % of the women had a dietary Ca intake >1300 mg/d. Only 17·1 % of women aged over 75 years achieved 1300 mg/d compared with 20·5 % of women aged less than 75 years (P = 0·0001 for the difference between the two groups). Conclusion Dietary intake of Ca is very low in European postmenopausal women. A greater awareness is needed to resolve this public health problem. [less ▲]

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See detailLow dose of dopamine infusion : hemodynamic and natriuretic responses
D'Orio, Vincenzo ULg; El-Allaf, D

in Clinical Pharmacology & Therapeutics (1984), 35

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See detailLow doses of FSH stimulate follicular growth and maturation in anestrous heifers.
Beckers, Jean-François ULg; Ballman-Wouters, P; Viviers-Donnay, I et al

in Theriogenology (1989)

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See detailLow flow regionalisation in the Walloon Region
Grandry, Maud ULg; Verstraete, Arnaud; Gailliez, Sébastien et al

Report (2012)

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See detailLow frequency electromagnetic waves in periodic structures
Guenneau, S.; Nicolet, A.; Geuzaine, Christophe ULg et al

in International Journal of Applied Electromagnetics and Mechanics (2004), 19

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See detailLow frequency rythms in human DNA sequences: A key to the organization of gene location and orientation?
Nicolay, Samuel ULg; Argoul, F.; Touchon, M. et al

in Physical Review Letters (2004), 93(10), 108101

We explore large-scale nucleotide compositional fluctuations of the human genome using multi- resolution techniques. Analysis of the GC content and of the AT and GC skews reveals the existence of rhythms ... [more ▼]

We explore large-scale nucleotide compositional fluctuations of the human genome using multi- resolution techniques. Analysis of the GC content and of the AT and GC skews reveals the existence of rhythms with two main periods of 110 20 kb and 400 50 kb that enlighten a remarkable cooperative gene organization. We show that the observed nonlinear oscillations are likely to display all the characteristic features of chaotic strange attractors which suggests a very attractive deterministic picture: gene orientation and location, in relation with the structure and dynamics of chromatin, might be governed by a low-dimensional nonlinear dynamical system. [less ▲]

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See detailLow GH hypophyseal adenomas with acromegaly exhibit undetectable levels of messenger RNA of normal GH
Pagesy, P.; Rentier-Delrue, Françoise ULg; Bresson, D. et al

in Annals of Endocrinology (1987), 48

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See detailLow incidence of anti-osteoporosis treatment after hip fracture.
Rabenda, Véronique ULg; Vanoverloop, Johan; Fabri, Valerie et al

in Journal of Bone & Joint Surgery. American Volume (2008), 90(10), 2142-8

BACKGROUND: Following hip fracture, pharmacologic treatment can reduce the rate of subsequent fragility fractures. The objective of the present study was to assess the proportion of patients who are ... [more ▼]

BACKGROUND: Following hip fracture, pharmacologic treatment can reduce the rate of subsequent fragility fractures. The objective of the present study was to assess the proportion of patients who are managed with bisphosphonates or selective estrogen-receptor modulators after hip fracture and to evaluate, among those managed with alendronate, the twelve-month compliance and persistence with treatment. METHODS: Data were gathered from health insurance companies and were collected by AIM (Agence Intermutualiste) for the Belgian National Social Security Institute (INAMI). We selected all postmenopausal women who had been hospitalized for a hip fracture between April 2001 and June 2004 and had not been previously managed with bisphosphonates. Patients who had received alendronate treatment after the hip fracture were categorized according to their formulation use during the follow-up study (daily, weekly, daily followed by weekly, or weekly followed by weekly). Compliance at twelve months was quantified with use of the medication possession ratio (i.e., the number of days of alendronate supplied during the first year of treatment, divided by 365). Persistence with prescribed treatment was calculated as the number of days from the initial prescription to a lapse of more than five weeks after completion of the previous prescription refill. The cumulative treatment persistence rate was determined with use of Kaplan-Meier survival curves. RESULTS: A total of 23,146 patients who had sustained a hip fracture were identified. Of these patients, 6% received treatment during the study period: 4.6% received alendronate, 0.7% received risedronate, and 0.7% received raloxifene. Bisphosphonate treatment was dispensed to 2.6% and 3.6% of the patients within six months and one year after the occurrence of the hip fracture, respectively. Among women who received alendronate daily (n = 124) or weekly (n = 182) and were followed for at least one year after the hip fracture, the twelve-month mean medication possession ratio was 67% (65.9% in the daily group and 67.7% in the weekly group). The analysis of persistence with treatment included a total of 726 patients (142 in the daily group, 261 in the weekly group, and 323 in the switch group). At twelve months, the rate of persistence was 41% and the median duration of persistence was 40.3 weeks. CONCLUSIONS: The vast majority of patients who experience a hip fracture do not take anti-osteoporotic therapy after the fracture. Furthermore, among patients who begin alendronate treatment after the fracture, the adherence to treatment decreases over time and remains suboptimal. [less ▲]

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See detailLow incidence of osteoporosis treatment after hip fracture
Rabenda, Véronique ULg; Mertens, Raf; Fabri, Valérie et al

in Osteoporosis International (2007, March), 18(Suppl.1), 72-73

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See detailLow molecular weight poly (2-dimethylamino ethylmethacrylate) polymers with controlled positioned fluorescent labeling: Synthesis, characterization and in vitro interaction with human endothelial cells.
Flebus, Luca ULg; Lombart, François ULg; Sevrin, Chantal et al

in International journal of pharmaceutics (2015), 478(1), 278-287

Poly (2-dimethylamino ethylmethacrylate) (PDMAEMA) is an attractive non-degradable polymer studied as nonviral vector for gene delivery but it can be also adopted for delivery of other biopharmaceutical ... [more ▼]

Poly (2-dimethylamino ethylmethacrylate) (PDMAEMA) is an attractive non-degradable polymer studied as nonviral vector for gene delivery but it can be also adopted for delivery of other biopharmaceutical drugs. As a parenteral carrier, the PDMAEMA free form (FF) might interact with tissues and cells. Few data are available on its selective internalization and efflux from cells, while the majority of studies published have followed the distribution of DNA complexed with PDMAEMA. In order to address polycation safety, the first aim was to synthesize by atom transfer radical polymerisation (ATRP) fluorescent labeled PDMAEMA of low molecular weight (Mw) (below 15kDa), controlling the position and density of fluorescein. The second goal was to analyze the possible difference in uptake and subcellular distribution of this labeled FF polycation between human umbilical vein endothelial cells (HUVEC) and hCMEC/D3 cells. These two cell lines have been chosen in order to detect selectivity towards the blood-brain barrier (BBB). In both cases, polycation was detected along the plasma membrane followed by progressive migration to the peri-nuclear region, where it overlapped with lysosomal structures. The analysis by fluorescence-activated cell sorting (FACS) of the PDMAEMA uptake by hCMEC/D3 cells showed a significant (p<0.05) inhibition (40%) in presence of 2-dexoxy-d-glucose inhibitor, a result supporting an energy-dependence mechanism(s). Cytotoxicity study showed that low Mw PDMAEMA (10kDa) lead to a minor cytotoxicity compared to the higher ones. As main conclusion this study highlights the similitude in cell trafficking of FF PDMAEMA and data previously reported for PDMAEMA/DNA complexes. [less ▲]

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See detailA low rate of trisomy 21 in twin-pregnancies: A cytogenetics retrospective study of 278 cases
Jamar, Mauricette ULg; Lemarchal, C.; Koulischer, Lucien ULg et al

in Genetic Counseling (2003), 14(4), 395-400

Detailed reference viewed: 27 (4 ULg)