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See detailMethod Development Strategies for the Enantioseparation of Drugs by Capillary Electrophoresis Using Cyclodextrins as Chiral Additives
Fillet, Marianne ULg; Hubert, Philippe ULg; Crommen, Jacques ULg

in Electrophoresis (1998), 19(16-17), 2834-40

General strategies for the development of capillary electrophoretic methods for the enantiomeric separation of basic, acidic or neutral drugs were developed. For all kinds of compounds, the use of a ... [more ▼]

General strategies for the development of capillary electrophoretic methods for the enantiomeric separation of basic, acidic or neutral drugs were developed. For all kinds of compounds, the use of a buffer made of 100 mM phosphoric acid adjusted to pH 3 with triethanolamine and containing anionic and/or uncharged cyclodextrin (CD) derivatives as chiral selectors was recommended. Two different optimization schemes depending on the acidic or basic character of the analytes, were elaborated. For most basic compounds present in cationic form at pH 3, enantiomeric separation could be achieved in the normal polarity mode. Different beta-cyclodextrin derivatives were first tested at a given concentration. Five derivatives were found to be particularly useful for enantioseparations in capillary electrophoresis (CE): the anionic carboxymethyl-beta-CD (CMCD) and sulfobutyl-beta-CD (SBCD) and the neutral dimethyl-beta-CD (DMCD), trimethyl-beta-CD (TMCD) and hydroxypropyl-beta-CD (HPCD). After selection of the most suitable CD, its concentration was optimized with respect to chiral resolution. If necessary, a further improvement in resolution could often be obtained for the enantiomers of cationic solutes by increasing the buffer pH from 3 to 5 using CMCD as chiral additive. Another possible alternative for enhancement in chiral resolution was the addition of metharlol or cyclohexanol to the buffer. For acidic drugs, essentially present in uncharged form at pH 3, and for neutral solutes, anionic CD derivatives such as SBCD or CMCD were first tested at a given concentration in the reversed polarity mode. Dual systems, based on the simultaneous addition of a charged CD (SBCD or CMCD) and a neutral CD (TMCD or DMCD), could then be investigated for resolution improvement. After optimization of the CD concentrations, the use of dual systems with CMCD at pH 5 could also be tested if necessary, especially for very weak acidic and neutral drugs. By applying these optimization strategies, 48 of the 50 drugs examined as model compounds could be fully enantioseparated by CE in short analysis times (usually less than 10 min). [less ▲]

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See detailMethod development strategies for the rapid enantioseparation of drugs by capillary electrophoresis using cyclodextrins as chiral additives
Fillet, Marianne ULg; Fotsing, L.; Piette, V. et al

in Journal de Pharmacie de Belgique (1998), 53

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See detailA Method for Applying LOTOS at an Early Design Stage and Its Application to the ISO Transport Protocol
Leduc, Guy ULg

in The OSI95 Transport Service with Multimedia Support (1994)

We propose a method applicable to the design of large and abstract LOTOS specifications. More precisely, this method explains how to generate a constrain-oriented specification which is an adequate ... [more ▼]

We propose a method applicable to the design of large and abstract LOTOS specifications. More precisely, this method explains how to generate a constrain-oriented specification which is an adequate abstract and modular starting point of a complex design process leading to implementation. It is illustrated on a substantial part of the ISO Transport Protocol class 4 which is considered as a complex and stable case study. Having proved the feasibility of the method on this protocol, it was then used to specify (parts of) the new transport protocol TPX of OSI95. [less ▲]

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See detailMethod for co-purification of equine neutrophil elastase and myeloperoxidase from a limited blood volume.
de la Rebière de Pouyade, Geoffroy ULg; Serteyn, Didier ULg; Deby, Ginette ULg et al

in Research in Veterinary Science (2009)

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities ... [more ▼]

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities. We describe the isolation of both enzymes from a same limited blood volume. Both MPO and elastase were extracted by crushing PMN isolated by centrifugation on a percoll-gradient from a 460ml blood collection. MPO and elastase were separated by an ionic exchange chromatography phase and further purified by gel filtration chromatography on Superdex 200 and 75, respectively. Enzymes were identified in the collected fractions by specific enzymatic assays. The final purity was verified by electrophoresis. Specific activity was improved to 19.92 and 34.3x for elastase (final yield: 340mug) and MPO (final yield: 130mug), respectively, during the procedure. Results show the possibility of isolating both enzymes from the same blood sample with a sufficient yield and purity for future studies on their implication and interaction during inflammatory diseases. [less ▲]

Detailed reference viewed: 37 (14 ULg)
See detailMethod for controlled radical polymerisation
Jérôme, Robert ULg; Creutz, Serge; Dubois, Philippe et al

Patent (1998)

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general ... [more ▼]

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general formula (I) is used in which the substituents (Z)m, (R)n and Y can have different values. [less ▲]

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See detailMethod for controlled radical polymerization
Jérôme, Robert ULg; Creutz, Serge; Dubois, Philippe et al

Patent (1998)

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general ... [more ▼]

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general formula (I) is used in which the substituents (Z)m, (R)n and Y can have different values. [less ▲]

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Peer Reviewed
See detailA method for estimating the migration time of plant species within the time range of 14C-Dating.
Juvigné, Etienne ULg; Bastin, B; Delibrias, G et al

in Quaternary International (1998), 47/48

Detailed reference viewed: 15 (0 ULg)
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See detailMethod for estimation of low outer membrane permeability to beta-lactam antibiotics
Lakaye, Bernard ULg; Dubus, Alice ULg; Joris, Bernard ULg et al

in Antimicrobial Agents and Chemotherapy (2002), 46(9), 2901-2907

The outer membrane of gram-negative bacteria plays a major role in beta-lactam resistance as it slows down antibiotic entry into the periplasm and therefore acts in synergy with beta-lactamases and efflux ... [more ▼]

The outer membrane of gram-negative bacteria plays a major role in beta-lactam resistance as it slows down antibiotic entry into the periplasm and therefore acts in synergy with beta-lactamases and efflux systems. Up to now, the quantitative estimation of low outer membrane permeability by the method of Zimmermann and Rosselet was difficult because of the secreted and cell surface-associated beta-lactamases. The method presented here uses the acylation of a highly sensitive periplasmic penicillin-binding protein (PBP) (BlaR-CTD) to assess the rate of beta-lactam penetration into the periplasm. The method is dedicated to measurement of low permeability and is only valid when the diffusion rate through the outer membrane is rate limiting. Cytoplasmic membrane associated PBPs do not interfere since they are acylated after the very sensitive BlaR-CTD. This method was used to measure the permeability of beta-lactamase-deficient strains of Enterobacter cloacae and Enterobacter aerogenes to benzylpenicillin, ampicillin, carbenicillin, cefotaxime, aztreonam, and cephacetrile. Except for that of cephacetrile, the permeability coefficients were equal to or below 10(-7) cm/s. For cephacetrile, carbenicillin, and benzylpenicillin, the outer membrane of E. cloacae was 20 to 60 times less permeable than that of Escherichia coli, whereas for cefotaxime, aztreonam, and ampicillin it was, respectively, 400, 1,000, and 700 times less permeable. The permeability coefficient for aztreonam is the lowest ever measured (P = 3.2 X 10(-9) cm/s). Using these values, the MICs for a beta-lactamase-overproducing strain of E. cloacae were successfully predicted, demonstrating the validity of the method. [less ▲]

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See detailA method for evaluating transport energy consumption in suburban areas
Marique, Anne-Françoise ULg; Reiter, Sigrid ULg

in Environmental Impact Assessment Review (2012), 33

Urban sprawl is a major issue for sustainable development. It represents a significant contribution to energy consumption of a territory especially due to transportation requirements. However, transport ... [more ▼]

Urban sprawl is a major issue for sustainable development. It represents a significant contribution to energy consumption of a territory especially due to transportation requirements. However, transport energy consumption is rarely taken into account when the sustainability of suburban structures is studied. In this context, the paper presents a method to estimate transport energy consumption in residential suburban areas. The study aimed, on this basis, at highlighting the most efficient strategies needed to promote awareness and to give practical hints on how to reduce transport energy consumption linked to urban sprawl in existing and future suburban neighborhoods. The method uses data collected by using empirical surveys and GIS. An application of this method is presented concerning the comparison of four suburban districts located in Belgium to demonstrate the advantages of the approach. The influence of several parameters, such as distance to work places and services, use of public transport and performance of the vehicles, are then discussed to allow a range of different development situations to be explored. The results of the case studies highlight that travelled distances, and thus a good mix between activities at the living area scale, are of primordial importance for the energy performance, whereas means of transport used is only of little impact. Improving the performance of the vehicles and favoring home-work give also significant energy savings. The method can be used when planning new areas or retrofitting existing ones, as well as promoting more sustainable life styles regarding transport habits. [less ▲]

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See detailA method for evaluating weaknesses and critical steps in the Radiation Treatment Process through Precursor Events reporting
LENAERTS, Eric ULg; DELGAUDINE, Marie ULg; COUCKE, Philippe ULg

Poster (2011, September)

objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains ... [more ▼]

objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains, functional Basic Responsibilities and levels of Severity of precursor events [less ▲]

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See detailA method for identification of non-linear multi-degree-of-freedom systems
Dimitriadis, Grigorios ULg; Cooper, Jonathan E

in Proceedings of the Institution of Mechanical Engineers - Part G - Journal of Aerospace Engineering (1998), 212(4), 287-298

System identification methods for non-linear aeroelastic systems could find uses in many aeroelastic applications such as validating finite element models and tracking the stability of aircraft during ... [more ▼]

System identification methods for non-linear aeroelastic systems could find uses in many aeroelastic applications such as validating finite element models and tracking the stability of aircraft during flight flutter testing. The effectiveness of existing non-linear system identification techniques is limited by various factors such as the complexity of the system under investigation and the type of non-linearities present. In this work, a new approach is introduced which can identify multi-degree-of-freedom systems featuring any type of non-linear function, including discontinuous functions. The method is shown to yield accurate identification of three mathematical models of aeroelastic systems containing a wide range of structural non-linearities. [less ▲]

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See detailMethod for identifying cows with mastitis by bulk genotyping of tank milk.
Georges, Michel ULg; Coppieters, Wouter ULg; Blard, Grégoire

Patent (2013)

Detailed reference viewed: 19 (1 ULg)
See detailMethod for increasing the bioavailability of inhaled compounds
Vanbever, Rita; Koussoroplis, Salomé; Cataldo, Didier ULg et al

Patent (2014)

Detailed reference viewed: 7 (1 ULg)
Peer Reviewed
See detailA method for isolation of intact, translationally active ribonucleic acid
Cathala, G.; Savouret, J. F.; Mendez, B. et al

in DNA (1983), 2(4), 329-35

A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 M guanidine monothiocyanate followed by direct ... [more ▼]

A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 M guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 M LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (less than 300 nucleotides) RNA species are recovered with a poor yield. [less ▲]

Detailed reference viewed: 91 (0 ULg)