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See detailThe influence of mechanical parameters on tissue differentiation and bone formation around immediately loaded implants in the bone chamber model
Vandamme, Katleen; Duyck, Joke; Geris, Liesbet ULg et al

in Proceedings of the first thematic workshop of the European Society of Biomechanics on Mechanobiology of Cells and Tissue Engineering (2005)

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See detailInfluence of medium composition on oxygen transfer rate in animal cell culture
Toye, Dominique ULg; Galifi, A.; Salmon, Thierry ULg et al

in Canadian Journal of Chemical Engineering (2010), 88(4), 671-676

Experiments were conducted in a 0.25m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate. Aqueous solutions, the composition of which mimics a mammalian cell ... [more ▼]

Experiments were conducted in a 0.25m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate. Aqueous solutions, the composition of which mimics a mammalian cell culture medium, are used. The effect on oxygen transfer rate of additives used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed, in the range of operating conditions (aeration rates) met in animal cell cultures. The mass transfer coefficient, the liquid viscosity and surface tension, and the bubble size distribution are measured as a function of liquid composition and of gas superficial velocity, allowing to decouple the effects of the different additive on kL and on a. [less ▲]

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See detailInfluence of medium composition on oxygen transfer rate in animal cell culture
Toye, Dominique ULg; Galifi, Alain; Salmon, Thierry ULg et al

in Proceedings of 8th World Congress on Chemical Engineering (2009)

Experiments were conducted in a 0.25 m diameter bubble column to investigate the effect of animal cell culture medium composition on oxygen transfer rate. Air is used as the dispersed phase. The gas ... [more ▼]

Experiments were conducted in a 0.25 m diameter bubble column to investigate the effect of animal cell culture medium composition on oxygen transfer rate. Air is used as the dispersed phase. The gas superficial velocity is varied between 0.4 and 2 cm/s (aeration rate ranging between 0.05 and 0.25 vvm) and the bubble column is thus operated in the homogenous regime. Aqueous solutions the composition (electrolyte, protein concentrations) of which mimics a mammalian cell culture medium) are used as the continuous phase. In particular, the effect on oxygen transfer rate of additive such as PVP, Pluronic F-68 which are usually used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed. For each composition, the mass transfer coefficient is measured by the "gas in – gas out" method using a fast response dissolved oxygen probe. Its increase with gas velocity is measured. The liquid viscosity and surface tension are experimentally determined as a function of culture medium composition. Bubble size distribution is measured at different scales using three experimental techniques: gas disengagement technique, two dual optical probes and photography, which lead to results in good qualitative agreement one with each other. The integrated analysis of these data allows to decouple the effects of the different additive on coefficient kL and on the interfacial area, a. [less ▲]

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See detailInfluence of melodic context on the perception of vocal pitch accuracy
Beeken, Manon; Morsomme, Dominique ULg; Larrouy, Pauline ULg

Poster (2014, May 27)

In order to categorize sung performances as “in tune” or “out of tune”, one can measure the deviation between the performance and the musical notation. Depending on the study, a deviation of a semitone ... [more ▼]

In order to categorize sung performances as “in tune” or “out of tune”, one can measure the deviation between the performance and the musical notation. Depending on the study, a deviation of a semitone (100 cents) or a quartertone (50 cents) is considered as “out of tune”. However, these values are arbitrary and the current study aims to define perceptual thresholds of pitch accuracy in a melodic context. For this purpose, melodic sequences were manipulated, from “in tune” (deviation of 0 cent) to “out of tune” (10 to 80 cents, in 10 cents steps). In a 2x2x2 design we systematically varied the conditions of: melodies (ascending/descending target-interval), type of errors (interval or tonality deviation), and direction of the deviation (enlargement or compression). The sequences were presented to 30 non-musicians using the method of limits procedure, in a test/retest paradigm. For each condition, they were asked to specify whether the presented singing performances were “in tune” or “out of tune”. The results showed that participants were consistent in their evaluation between the test and the retest. The pitch accuracy threshold (M = 29 cents, ES = .75) was not influenced by the melody, the type of errors, and the direction of the deviation. This study highlights the ability of non-musicians to perceive small pitch deviations (less than a quartertone) in a melodic context. This finding elucidates on the concept of pitch accuracy and therefore yields the opportunity to revise objective tools for the evaluation of singer pitch accuracy. [less ▲]

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See detailInfluence of Melting and Rheological Properties of Fatty Binders on the Melt Granulation Process in a High-Shear Mixer
Evrard, Brigitte ULg; Amighi, K.; Beten, D. et al

in Drug Development & Industrial Pharmacy (1999), 25(11), 1177-84

The preparation of granules by melt granulation was investigated using a laboratory-scale high-shear mixer (Pellmix PL 1/8) and binary mixtures containing lactose and different lipidic binders, namely ... [more ▼]

The preparation of granules by melt granulation was investigated using a laboratory-scale high-shear mixer (Pellmix PL 1/8) and binary mixtures containing lactose and different lipidic binders, namely, Compritol 888, Cutina HR, or Precirol ATO5. [less ▲]

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See detailInfluence of membrane-bound tumor necrosis factor (TNF)-alpha on obesity and glucose metabolism
Vörös, Gabor; Maquoi, Erik ULg; Collen, Désiré et al

in Journal of Thrombosis and Haemostasis [=JTH] (2004), 2(3), 507-513

OBJECTIVES: To investigate the influence of transmembrane tumor necrosis factor (TNF)-alpha on adipose tissue development and insulin-mediated glucose metabolism. METHODS AND RESULTS: TNF-alpha and ... [more ▼]

OBJECTIVES: To investigate the influence of transmembrane tumor necrosis factor (TNF)-alpha on adipose tissue development and insulin-mediated glucose metabolism. METHODS AND RESULTS: TNF-alpha and lymphotoxin-alpha-deficient mice expressing non-cleavable transmembrane TNF-alpha (Tg-tmTNF-alpha) and TNF-alpha/lymphotoxin-alpha double knockout (control) mice were kept on high-fat diet for 15 weeks. The food intake and feeding efficiency of Tg-tmTNF-alpha mice were significantly higher compared with control mice. At the end of the study, Tg-tmTNF-alpha mice had a significantly higher total body weight, as well as subcutaneous and gonadal adipose tissue mass. Histological analysis revealed that the expression of Tg-tmTNF-alpha resulted in a significantly increased adipocyte area and blood vessel density. Plasma leptin levels correlated positively with adipose tissue mass. The plasma levels of total cholesterol and HDL-cholesterol were significantly increased and LDL-cholesterol levels significantly decreased in Tg-tmTNF-alpha mice. Fasting blood glucose and plasma insulin levels were not different between the two genotypes and intraperitoneal glucose and insulin tolerance tests did not show significant differences. CONCLUSIONS: Transmembrane TNF-alpha enhances adipose tissue formation without altering insulin-mediated glucose metabolism in mice with nutritionally induced obesity. [less ▲]

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See detailInfluence of mesoporosity in hematite films on water splitting efficiency
Toussaint, Caroline ULg; Cloots, Rudi ULg; Henrist, Catherine ULg

Poster (2014)

Solar energy is inexhaustible but variable during the day and the seasons. Photoelectrolysis of water (water splitting) convert this energy into hydrogen to obtain an energy that can be stored and ... [more ▼]

Solar energy is inexhaustible but variable during the day and the seasons. Photoelectrolysis of water (water splitting) convert this energy into hydrogen to obtain an energy that can be stored and transported on demand. Hematite is a promising material for the photoanode in water splitting because of its high stability in water, cheapness, abundance and its band gap that enables the absorption of visible light (Eg: 2,1eV). Nevertheless, hematite has also some drawbacks including a short diffusion length of holes and a bad electronic conductivity. We have implemented spin coating and templating to produce doped mesoporous hematite films. The nanostructuration can improve the performances in water splitting by reducing the diffusion length of holes and increasing the specific surface between the film and the electrolyte. To suppress the collapse of the mesoporosity at high temperature (requested for dopant activation), we have used a temporary silica confinement scaffold that reduces the crystallite growth. To show the impact of the nanostructure, we have compared three films (mesoporous, collapsed and dense) in terms of hematite content (elemental analysis), nanostructure (electron microscopy), crystallinity (X-ray diffraction) and water splitting efficiency. We have also test two thermal treatments. This study highlights the effect of the effective interface with the electrolyte, through the preservation or not of open porosity and the different evolutions of the nanostructures as a function of the heat treatment. [less ▲]

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See detailInfluence of methanol on the enantioresolution of antihistamines with carboxymethyl-beta-cyclodextrin in capillary electrophoresis
Van Eeckhaut, A.; Detaevernier, M. R.; Crommen, Jacques ULg et al

in Electrophoresis (2004), 25(16), 2838-2847

According to the model of Wren and Rowe, the separation between two enantiomers in capillary electrophoresis (CE) decreases if an organic modifier is added to the run buffer containing a neutral ... [more ▼]

According to the model of Wren and Rowe, the separation between two enantiomers in capillary electrophoresis (CE) decreases if an organic modifier is added to the run buffer containing a neutral cyclodextrin (CD) in a concentration below its optimal value in a solvent-free system. In previous work, however, it was observed that the addition of methanol to the background electrolyte (BGE) containing not charged carboxymethyl-beta-CD in a concentration below its optimal value, increased the enantioresolution of dimetindene maleate. The enantioresolution decreased when other organic modifiers (ethanol, isopropanol or acetonitrile) were added and/or when other neutral (beta-CD, hydroxypropyl-beta-CD) or chargeable (carboxyethyl-beta- and succinyl-beta-CD) CDs were used. In this CE study further attempts are made to elucidate the observed phenomena through investigating other basic drugs. The effect of organic modifier and CD concentration on the enantioseparation was studied by means of central composite designs. It is shown that obtaining this increase in enantioresolution depends upon the type of CD, the type of organic modifier, and the structure of the analytes. It was also observed that small differences in the structure of the analytes or the CD could have an influence on the enantioresolution. The addition of methanol also resulted in different effects on the resolution of closely related analytes. [less ▲]

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See detailInfluence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate.
Carly, F.; Niu, H.; Delvigne, Frank ULg et al

in Journal of industrial microbiology & biotechnology (2016), 43(4), 517-23

High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen ... [more ▼]

High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen limitation in bioreactor. In the literature, there is lack of report about AOX1 promoter regulation with regard to dissolved oxygen level (DO). Therefore, in this work, chemostat cultures were performed to investigate the cell growth, metabolism and regulation of the AOX1 promoter (pAOX1) regarding co-feeding rate of optimized methanol/sorbitol mixture (methanol fraction 0.60 C-mol/C-mol) using a P. pastoris Mut+/pAOX1-lacZ strain. The oxygen transfer rates (OTR) in bioreactor were kept in the range of typical values of large bioreactor, i.e., 4-8 g/(L h) if DO equals 30 % saturation or 5-10 g/(L h) if DO nears zero. For DO >0, an increase of the carbon fed led to an increase of pAOX1 induction. By contrast, when dissolved oxygen was completely depleted, methanol accumulated, causing a 30 % decrease of pAOX1 induction. However, this decrease is more likely to be lined to methanol accumulation than to low level of dissolved oxygen (<4 % DO). Methanol/sorbitol co-feeding allowed cells to adapt to oxygen transient limitations that often occur at industrial scale with reduced effect on pAOX1 induction. The optimal feeding rate tested here was 6.6 mmol C (DCW h)(-1) at an OTR of 8.28 g O2(L h)(-1) with over fivefold pAOX1 induction (probably directly associated with target protein productivity) compared with previous work. [less ▲]

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See detailInfluence of methyl-b-cyclodextrin on the relase kinetics of inulin encapsulated in bioadhesive liposomes
Piel, Géraldine ULg; Boulmedarat, Laila; Bochot, Amélie et al

Poster (2004, May)

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See detailThe influence of micro-motion on the tissue differentiation around immediately loaded cylindrical turned titanium implants.
Duyck, Joke; Vandamme, K.; Geris, Liesbet ULg et al

in Archives of Oral Biology (2006), 51(1), 1-9

OBJECTIVE: The aim of this study was to evaluate the effect of various degrees of implant displacement on the tissue differentiation around immediately loaded cylindrical turned titanium implants. DESIGN ... [more ▼]

OBJECTIVE: The aim of this study was to evaluate the effect of various degrees of implant displacement on the tissue differentiation around immediately loaded cylindrical turned titanium implants. DESIGN: The experiments were conducted in repeated sampling bone chambers placed in the tibia of 10 rabbits. Tissues could grow into the bone chambers via perforations. Due to its double structure, tissues inside the chamber could be harvested leaving the chamber intact. This allowed several experiments within the same animal. The chambers contained a cylindrical turned titanium implant that was loaded in a well-controlled manner. In each of the 10 chambers, four experiments were conducted with the following test conditions: immediate implant loading by inducing 0 (control), 30, 60 and 90 microm implant displacement, 800 cycles per day at a frequency of 1 Hz, twice a week during a period of 6 weeks. Histological and histomorphometrical analyses were performed on methylmethacrylate histological sections. An ANOVA was conducted on the dataset. RESULTS: The total tissue volume was significantly lowest in the unloaded control condition. The bone volume fraction on the other hand, was significantly larger in the unloaded and 90 microm implant displacement, compared to the 30 microm implant displacement. Bone density increased with increasing micro-motion with significantly higher values for the 60 microm- and 90 microm-test conditions compared to the unloaded situation. The chance to have bone-to-implant contact decreased in case of micro-motion at the tissues-implant interface. CONCLUSION: The magnitude of implant displacement had a statistically significant effect on the tissue differentiation around immediately loaded cylindrical turned titanium implants. Implant micro-motion had a detrimental effect on the bone-to-implant contact in an immediate loading regimen. [less ▲]

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See detailInfluence of microfabric evolution on the retention behaviour of compacted clayey soils
Dieudonné, Anne-Catherine ULg; Della Vecchia, Gabriele; Charlier, Robert ULg et al

in Khalili, Nasser; Russel, Adrian; Khoshghalb, Arman (Eds.) Unsaturated Soils: Research and Applications (2014)

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See detailInfluence of microstructure on the thermal conductivity of magnetoresistive La0.7Ca0.3MnO3/Mn3O4 manganite/insulating oxide polycrystalline bulk composites
Mucha, J.; Vertruyen, Bénédicte ULg; Misiorek, H. et al

in Journal of Applied Physics (2009), 105(6), 063501

We report the temperature dependence of the thermal conductivity kappa(T) of bulk polycrystalline composite samples containing a magnetoresistive manganite (La0.7Ca0.3MnO3) and an electrically insulating ... [more ▼]

We report the temperature dependence of the thermal conductivity kappa(T) of bulk polycrystalline composite samples containing a magnetoresistive manganite (La0.7Ca0.3MnO3) and an electrically insulating phase (Mn3O4). The sample porosity is shown to be a significant parameter affecting the experimental data: after porosity correction the curves display the characteristics of an ideal composite. A fit of the kappa(T) curves at low temperature using the Debye model enables the mean free path of phonons scattered on "boundaries" to be determined. The values are on the order of the grain size but are influenced by the grain arrangement and the presence of twins. [less ▲]

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See detailThe influence of mineral and organic long-term fertilization upon the floristic composition of Festuca rubra L.-Agrostis capillaris L. grassland in Apuseni mountains, Romania
Pacurar, Florin; Rotar, Ioan; Bogdan, Anca Dorina et al

in Journal of Food, Agriculture & Environment (2012), 10(1), 866-879

The semi-natural grasslands in Apuseni Mountains are particularly important for the sustainable development of this region. The future development strategies must take into consideration the conservation ... [more ▼]

The semi-natural grasslands in Apuseni Mountains are particularly important for the sustainable development of this region. The future development strategies must take into consideration the conservation of grassland plant diversity. In Apuseni Mountains there are numerous caves and a traditional landscape, these forming the area’s wealth. Their joining with agro-tourism and performing a sustainable agriculture would significantly level up the wellbeing of the local population, who are in need of new income sources considering that the wood resources are more and more reduced. In Gârda de Sus there are considerable areas of semi-natural grasslands which lately have been traditionally used. Nowadays, some of them have been abandoned and others irrationally intensified. Therefore, the objective of this paper was to evaluate the effects of mineral and organic fertilization upon the sward in order to recommend certain versions which have minor repercussions upon the plant diversity. The research was carried out in Apuseni Mountains, in an experimental field with two experiences: one with mineral fertilizers (T1 control, T2 50N25P25K, T3100N50P50K, T4 150N75P75K) and the other with organic fertilizers (T1 control, T2 10 t ha-1 manure, T3 20 t ha-1 manure, T4 30 t ha-1 manure). Administrating mineral fertilizers on the Festuca rubra L.- Agrostis capillaris L. grassland induced the installation of Festuca rubra L. and Trisetum flavescens L. at 50N25P25K treatment, whereas 100N50P50K and 150N75P75K treatments induced the installation of Agrostis capillaris L. and Trisetum flavescens L. grassland subtype. The plant diversity decreased as the system was intensified, especially at the treatment with 100N50P50K and 150N75P75K. Administrating 50N25P25K did not induce any significant changes in plant diversity. Giving organic fertilizers caused installation of Festuca rubra L. and Trisetum flavescens L. at the treatment using 10 t ha-1 manure and Trisetum flavescens L. at using 20 and 30 t ha-1 manure. The treatment with 10 t ha-1 manure induced a growth of the plant diversity and by raising the manure quantity did not cause important changes with respect to the species’ number. For future management actions that are meant to maintain the plant diversity in Apuseni Mountains, the organic fertilization with 10 t ha-1 manure quantities annually administrated or once in two years is recommended first of all, and, secondly, the fertilization with 50N25P25K performed annually or once in two years. [less ▲]

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See detailInfluence of moderate temperatures on myristoyl-CoA metabolism and acyl-CoA thioesterase activity in the psychrophilic antarctic yeast Rhodotorula aurantiaca.
Sabri, Ahmed ULg; Bare, G.; Jacques, Philippe ULg et al

in Journal of Biological Chemistry (2001), 276(16), 12691-6

The inability of psychrophilic microorganisms to grow at moderate temperatures (>20 degrees C) presently represents an unresolved thermodynamic paradox. Here we report for the psychrophilic yeast ... [more ▼]

The inability of psychrophilic microorganisms to grow at moderate temperatures (>20 degrees C) presently represents an unresolved thermodynamic paradox. Here we report for the psychrophilic yeast Rhodotorula aurantiaca A19, isolated from Antarctic ice, that the inability to grow at temperatures close to 20 degrees C is associated with profound alterations in cell morphology and integrity. High performance liquid chromatography analysis of the intracellular acyl-CoA esters revealed an abnormal accumulation of myristoyl-CoA (C14-CoA) in cells cultivated close to the nonpermissive temperature. Its concentration (500 microm) was found to be 28-fold higher than in cells cultivated at 0 degrees C. If one considers its ability to disrupt membrane bilayers and to inhibit many cellular enzymes and functions, intracellular myristoyl-CoA accumulation in the psychrophile R. aurantiaca represents one of the principal causes of growth arrest at moderate temperatures. Intracellular acyl-CoA concentrations are believed to be regulated by thioesterase activity. Thus in an attempt to explore the mechanism by which temperature disrupts myristoyl-CoA metabolism, we isolated and characterized a long chain acyl-CoA thioesterase. The monomeric 80-kDa thioesterase from the psychrophilic yeast shows a very strong specificity for myristoyl-CoA. The affinity for substrate and the catalytic efficiency of the thioesterase are optimal below 5 degrees C (temperatures habitually experienced by the strain) and dramatically decrease with increasing temperature. The loss of affinity for substrate is related to the intracellular increase of myristoyl-CoA concentration. Our observations reveal one of the probable mechanisms by which temperature fixes the limit of growth for this psychrophilic yeast. [less ▲]

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See detailInfluence of monopalmitin on the crystallization behaviour of palm oil
Verstringe, Stefanie; Danthine, Sabine ULg; Depypere, Frederic et al

Conference (2012, May)

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See detailInfluence of monopalmitin on the isothermal crystallization mechanism of palm oil
Verstringe, S.; Danthine, Sabine ULg; Blecker, Christophe ULg et al

in Food Research International (2013), 51(1), 344-353

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See detailInfluence of month of birth on growth performance and morbidity incidence in Belgian Blue cattle
de Behr, V.; Cabaraux, Jean-François ULg; Hornick, Jean-Luc ULg et al

in Book of absracts of the 52nd Annual Meeting of the European Association for Animal Production (2001)

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See detailInfluence of Morphine on the Rabbit Gastrointestinal Tract
Deflers, Hélène ULg; Bolen, Géraldine ULg; Gandar, Frederic ULg et al

Conference (2014, October 20)

Detailed reference viewed: 43 (15 ULg)