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See detailInvestigation of biological markers to evaluate the adaptation of the newborn Belgian Blue calf to the extrauterine life. A pilot study.
Ronzoni, Anna ULg; Theron, Léonard ULg; De Marchin, Emmanuelle et al

in Dalin, Göran (Ed.) 15th International Conference on Production Diseases in Farms Animals, Book of Abstracts (2013, June)

Adaptation to extrauterine life and immunity transfer at calving are more at stake when dealing with highly productive breeds such as the Belgian Blue Cattle Breed (BBCB). The purpose of the present pilot ... [more ▼]

Adaptation to extrauterine life and immunity transfer at calving are more at stake when dealing with highly productive breeds such as the Belgian Blue Cattle Breed (BBCB). The purpose of the present pilot study was to evaluate different blood markers, from the birth to the first week of life in BBCB calves, in order to investigate fundamental aspects of the adaptation to the extrauterine life as passive immunity transfer and early energy metabolism. Eighty calves coming from 10 different farms located in Wallonia (Belgium) were enrolled. A clinical examination was performed to verify viability and maturity at birth. Blood samples were collected at birth, day 1 and day 3 to 7. Passive immunity transfer analysis included serum and colostrum immunoglobulin G (IgG) concentration, and plasmatic selenium concentration (Se). Glycemia, serum insulin and cortisol concentration have been considered for the energy metabolism analysis. The data collected were statistically analysed using logistic regression and unpaired t-test. All calves were born alive, viable and mature by caesarean section. Mortality rate was 0% at one week and 5% at three months. Only 33% of calves had an IgG concentration >16g/L after one week of age, while only 34% of colostra had an IgG concentration >70g/L. Glycemia and serum insulin concentration at birth were significantly lower than other breeds, and plasmatic selenium concentration were coherent with low supplemented dams. We compared calves across IgG concentration classes (>16 g/L vs <16g/L). The crosssectional analysis of the risk of FPT when given low IgG concentration colostra has shown a non significant odd ratio (OR 1,058 and 0,39116 g/L) (p<0,001). No significant difference has been shown between cortisol and glycemia (p=0,3) nor insulin and glycemia (p=0,2) at birth. These data suggest a significant difference in BBCB calves on several metabolic markers, but they also revealed that immunity transfer and micronutrition of pregnant hyper-productive dams remain a challenge in modern farms. [less ▲]

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See detailInvestigation of blood oxidant/antioxidant markers in healthy competition horses : effect of discipline and gender.
Kirschvink, N.; De Moffarts, Brieuc; Farnir, Frédéric ULg et al

in 7th International Conference on Equine Exercise Physiology (2006)

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See detailInvestigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds
Kirschvink, Nathalie; de Moffarts, Brieuc; Farnir, Frédéric ULg et al

in Equine Veterinary Journal. Supplement (2006), 36

REASONS FOR PERFORMING STUDY: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently ... [more ▼]

REASONS FOR PERFORMING STUDY: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently recommended. However, it is unknown whether there is a specific need for antioxidants according to performance, breed, gender or age. OBJECTIVES: To assess whether breed-, gender- and age-related differences of blood oxidant/antioxidant markers occur in competition horses. METHODS: Healthy horses (n = 493) underwent oxidant/ antioxidant blood marker determination. Vitamin E, lipophilic antioxidant capacity (ACL), ascorbic acid (AA), glutathione (GSH, GSSG), gluthione peroxidase (GPx), superoxide dismutase (SOD), selenium (Se), copper (Cu), zinc (Zn), lipid peroxides (Pool), oxidised proteins (Protox) were determined, as well as magnesium (Mg), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), packed cell volume (PCV) and haemoglobin (Hb). A mixed linear model assessed the effect of breed, gender and age category. P<0.05 was considered significant. RESULTS: Thoroughbreds showed the highest values of vitamin E, ACL, GPx, PCV and Hb, whilst standardbreds had the highest values of AA and LDH. Jumping horses had the highest Protox values. Females had significantly higher SOD values, whereas most of the other markers were higher in stallions and geldings. Horses age 2-6 years had higher AA, SOD and LDH values than horses age >6 years. Correlation analyses were positive and significant between vitamin E and GPx, VitE and ACL, Se and GPx, Cu and Pool and negative between Pool and vitamin E, Pool and ACL, Protox and GPx, Protox and vitamin E. CONCLUSIONS: Blood oxidant/ antioxidant status of horses is influenced by breed, gender and age. The correlation analyses suggest synergistic relations between GPx, vitamin E and Se and an antagonistic relation between Protox-GPx, Protox-vitamin E, and Pool-vitamin E. POTENTIAL RELEVANCE: The results of this investigation provide definition of the specific need for antioxidants and vitamins in competition horses. [less ▲]

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See detailInvestigation of body condition score and insulin sensitivity (RQUICKY) in downer cow syndrome
Bonvoisin, Marie ULg; Rollin, Frédéric ULg; Lebreton, Pascal et al

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

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See detailInvestigation of carbohydrate binding property of a fungal lectin from Xerocomus chrysenteron and potential use on Myzus persicae aphid.
Jaber, Karimi; Cuartero Diaz, Gaetan; Haubruge, Eric ULg et al

in Communications in Agricultural and Applied Biological Sciences (2008), 73(3), 629-38

In recent years encoding insecticidal Lectins have been suggested as one of the promising methods against insect pests and have been engineered successfully into a variety of crops including wheat, rice ... [more ▼]

In recent years encoding insecticidal Lectins have been suggested as one of the promising methods against insect pests and have been engineered successfully into a variety of crops including wheat, rice, tobacco and potatoes. Xerocomus chrysenteron Lectin (XCL) has a high hemagglutinating activity and results obtained from sugar specificity assay showed to have specific affinity to Galactose and N-Acetylgalactosamine (GalNAc). In previous studies, XCL was shown to have negative effects on some insect pests, including aphids. In the present study, the effects of different carbohydrates including D-glucose, D-mannose, D-galactose and GalNAc, associated with 0.1% XCL (w/v) in artificial diet was investigated to assess the evolution of the lectin toxicity toward Myzus persicae aphid during 7 days. M. persicae, a polyphagous aphid, showed no significant differences of mortality when fed with the XCL lectin associated with Glucose and Mannose or fed on XCL diet only. At the opposite, the mortality rates related to artificial diet supplemented with Galactose or GalNAc and XCL were significantly reduced. There was then a significant mortality difference between M. persicae fed on an artificial diet incorporated specific carbohydrate binding Lectin with those fed with lectin only. The potential use of this particular fungal Lectin (XCL) with more specific carbohydrate binding will be discussed in relation to the development of bio-insecticide and integrated pest management. [less ▲]

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See detailInvestigation of clostridium difficile interspecies relatedness using multilocus sequence typing, multilocus variable-number tandem-repeat analysis and antimicrobial susceptibility testing
Rodriguez Diaz, Cristina ULg; Avesani, Véronique; Taminiau, Bernard ULg et al

in Veterinary Microbiology (2015)

Multilocus sequence typing (MLST), multilocus variable-number tandem-repeat analysis (MLVA) and antimicrobial susceptibility were performed on 37 animal and human C. difficile isolates belonging to 15 ... [more ▼]

Multilocus sequence typing (MLST), multilocus variable-number tandem-repeat analysis (MLVA) and antimicrobial susceptibility were performed on 37 animal and human C. difficile isolates belonging to 15 different PCR-ribotypes in order to investigate the relatedness of human and animal isolates and to identify possible transmission routes. MLVA identified a total of 21 different types while MLST only distinguished 12 types. Identical C. difficile strains were detected in the same animal species for PCR-ribotypes 014, 078, UCL 16U and UCL 36, irrespective of their origin or the isolation date. Non clonal strains were found among different hosts; however, a high genetic association between pig and cattle isolates belonging to PCR-ribotype 078 was revealed. MLVA also showed genetic differences that clearly distinguished human from animal strains. For a given PCR-ribotype, human and animal strains presented a similar susceptibility to the antimicrobials tested. All strains were susceptible to vancomycin, metronidazole, chloramphenicol and rifampicin, while PCR-ribotypes 078, UCL 5a, UCL 36 and UCL 103 were associated with erythromycin resistance. The data suggest a wide dissemination of clones at hospitals and breeding-farms or a contamination at the slaughterhouse, but less probability of interspecies transmission. However, further highly discriminatory genotyping methods are necessary to elucidate interspecies and zoonotic transmission of C. difficile. [less ▲]

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See detailInvestigation of cytokine expression in canine idiopathic pulmonary fibrosis
Krafft, Emilie ULg; Heikkilä, HP; Vanherberghen, Morgane ULg et al

in Proceeding of 29th VCRS Symposium (2011, November 02)

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See detailInvestigation of defect levels in Mg-doped GaN Schottky structures by thermal admittance spectroscopy
Nguyen, Ngoc Duy ULg; Germain, Marianne; Schmeits, Marcel et al

in Physica Status Solidi B. Basic Research (2001), 228

Schottky structures based on Mg-doped GaN layers grown by metalorganic chemical vapor deposition (MOCVD) on sapphire substrate are studied by thermal admittance spectroscopy from 90 K to room temperature ... [more ▼]

Schottky structures based on Mg-doped GaN layers grown by metalorganic chemical vapor deposition (MOCVD) on sapphire substrate are studied by thermal admittance spectroscopy from 90 K to room temperature. Evidence of two impurity levels results from the analysis of the observed peaks in the conductance curves, whose positions and strengths are temperature dependent. The experimental results are analyzed within a detailed theoretical study of the steady-state and small-signal electrical characteristics of the structure. Numerical simulations are based on the solution of the basic semiconductor equations for the structure consisting of two Schottky diodes connected back-to-back by a conduction channel formed by the GaN layer. [less ▲]

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See detailInvestigation of defect levels in Mg-doped GaN Schottky structures by thermal admittance spectroscopy
Nguyen, Ngoc Duy ULg; Germain, Marianne; Schmeits, Marcel et al

in Ponce, Fernando; Bell, Abigail (Eds.) ICNS-4: Proceedings of The Fourth International Conference on Nitride Semiconductors (2001)

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See detailInvestigation of Demagnetization in HTS Stacked Tapes Implemented in Electric Machines as a Result of Crossed Magnetic Field
Baghdadi, M.; Ruiz, H. S.; Fagnard, Jean-François ULg et al

in IEEE Transactions on Applied Superconductivity (2015), 25(3), 6602404

This paper investigates the practical effectiveness of employing superconducting stacked tapes to superconducting electric machinery. The use of superconducting bulks in various practical applications has ... [more ▼]

This paper investigates the practical effectiveness of employing superconducting stacked tapes to superconducting electric machinery. The use of superconducting bulks in various practical applications has been addressed extensively in the literature. However, in practice, dramatic decrease in magnetization would occur on superconducting bulks due to the crossed field effect. In our study, we employed the superconducting stacked tapes in a synchronous superconducting motor, which was designed and fabricated in our laboratory, aiming to lessen demagnetization due to crossed field effect in comparison with superconducting bulks. Applying the transverse AC field, the effects of frequency, amplitude, and number of cycles of the transverse magnetic field are discussed. Furthermore, a stack of 16 layers of superconducting tapes is modelled and the consequences of applying the crossed magnetic field on the sample are evaluated. The confrontation between experiments and simulation allows us to thoroughly understand the crossed field effects on stacked tapes. At the end, a preventive treatment, based on the shielding characteristic of superconductor and materials with high permeability, i.e. $mu$-metal and metalic glass, is suggested. On the other hand, the shielding feature of aforementioned materials will hinder the penetration of magnetic field and, consequently, reduction of the demagnetization will be attained. [less ▲]

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See detailInvestigation of diaphragm deflection of an absolute MEMS capacitive polysilicon pressure sensor
Walk, C; Goehlich, A; Giese, A et al

in Proc. SPIE Microtechnologies, Smart Sensors, Actuators, and MEMS VII (2015, May)

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See detailInvestigation of differential virus transmission efficiency in aphid strains: from biological to proteomic assays
Bosquée, Emilie ULg; Francis, Frédéric ULg

Poster (2014, June)

Aphids are well known for their variable efficiency in virus transmission to host plant. Not only aphid species but also aphid strain is an important factor to consider in the plant-aphid-virus ... [more ▼]

Aphids are well known for their variable efficiency in virus transmission to host plant. Not only aphid species but also aphid strain is an important factor to consider in the plant-aphid-virus interactions. In order to investigate these relations, different approaches should be developped from biological observations assessing the virus transmission efficiency to molecular tasks to better know the involved mechanisms in the aphid vector. After a period of virus acquisition on an artificial diet (depending on the kind of transmission, permanent or non permanent ways), the virus transmission rate were assayed and followed by the determination of the presence of virus in the aphid by PCR or immudetection. The aphids were then collected to investigate the variation of aphid proteome and potential involvement in virus interactions. Changes in protein expressions were investigated using a 2D-DIGE approach comparing most and least efficient aphid strains selecting different aphid – virus models (PVY and PLRV in potato, BYDV in wheat, CMV on tobacco) . The proteome changes were analysed using the samespot software. Protein spots with significant up- and down expressions were then mechanically picked, trypsin digested and analysed by Maldi-Tof-Tof. Significantly changed proteins were classify according to metablic pathways and biological functions. Interesting proteins found to probably interact with virus were not only detected from aphid but also from related bacterial symbionts. Theses results were discussed in order to better understand virus – vector relations and potentially promote new ways to control virus transmitted by hemipteran pest. [less ▲]

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See detailInvestigation of DyBa2CU3O7-d superconducting domains grown by the infiltration technique starting with small size Dy-211 particles
Mathieu, Jean-Philippe; Koutzarova, T.; Rulmont, André ULg et al

in Superconductor Science and Technology (2005), 18(2 Sp. Iss. SI), 136-141

An infiltration and growth process is here used as an alternative to the classical top-seeded melt-textured growth process for the production of Dy-123 single-domains with finely dispersed small size Dy ... [more ▼]

An infiltration and growth process is here used as an alternative to the classical top-seeded melt-textured growth process for the production of Dy-123 single-domains with finely dispersed small size Dy-211 particles. The starting materials are the 211-particles and a barium and copper rich liquid phase precursor. The infiltration and growth process allows for controlling both the spatial and size distribution of the 211-particles in the final superconducting 123-single-domain. The main parameters (set-ups, maximum processing temperature with respect to the peritectic temperature, nature of reactant, porosity of the 211-preform) of the infiltration and growth process are discussed. Moreover, different processes of chimie douce are shown in order to produce Dy-211 particles with controlled shape and size, particles that can be used as precursors for the infiltration and growth process. [less ▲]

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See detailInvestigation of edge effect on wood density in recent tropical forest edges in Yangambi Man and Biosphere Reserve
de Haulleville, Thalès ULg; Bogaert, Jan ULg; Beeckman, Hans

Conference (2013, April)

Rapid development of lumbering activities in tropical forested areas has an important impact on carbon stocks and landscape configuration. Indeed, lumbering tend to fragment forest patches, extend edges ... [more ▼]

Rapid development of lumbering activities in tropical forested areas has an important impact on carbon stocks and landscape configuration. Indeed, lumbering tend to fragment forest patches, extend edges length, and expose core areas to non-forested ecosystems. These exposed core areas then undergo new constraints that can lead to a shift in their ecological features, called the edge effect. We investigate the possible presence of an edge effect on wood density in Yangambi reserve. Wood density (along with diameter and height) is a key feature for biomass estimations in tropical forests. Thus, fluctuations of wood density in forest edges could lead to incorrect estimations of carbon stocks. Ten 1ha plots were installed in core and recent (5 to 10 years) forest¿s edges. Trees were measured and wood samples were collected for density analysis. We focused on 2 main research questions: 1) Does the edge effect induce the emergence of species with a higher or lower wood density in edge area? 2) Does wood density differ amongst individuals of the same specie in core and in edge area? While the data is still being explored as we write, preliminary results seem to indicate that there is no significant difference between the overall mean wood density in edge and core plots. Density differences amongst species are still under exploration. If this behaviour still holds, this would imply that the fragmentation of a forested landscape has no short-term effect on the overall forest wood density and thus, no effect on biomass estimations. [less ▲]

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See detailInvestigation of eighteenth-century Prussian blue pigments by PDF analysis
Samain, Louise ULg; Martinetto, Pauline; Bordet, Pierre

Poster (2012, October 30)

Prussian blue, a hydrated iron(III) hexacyanoferrate(II) complex, is a synthetic pigment discovered in Berlin in 1704. Because of both its highly intense color and its low cost, Prussian blue was widely ... [more ▼]

Prussian blue, a hydrated iron(III) hexacyanoferrate(II) complex, is a synthetic pigment discovered in Berlin in 1704. Because of both its highly intense color and its low cost, Prussian blue was widely used as a pigment in paintings until the 1970's. The early preparative methods were rapidly recognized as a contributory factor in the fading of the pigment [1], a fading already known by the mid-eighteenth century. The eighteenth-century methods are based on the calcination of dried blood to produce a potassium hexacyanoferrate complex, which is the first of two essential reactants for synthesizing Prussian blue. The second reactant is an iron salt. We successfully reproduced two typical eighteenth-century empirical recipes [2]. The resulting pigments were of variable color quality, ranging from intense blue to blue-gray or blue-green, and exhibit broadened or inexistent Bragg peaks. High-energy powder X-ray diffraction experiments were performed at the ID11 beamline at ESRF, Grenoble, France. The pair distribution function (PDF) of the pure Prussian blue pigments was refined with a three-phase model, in order to take into account the vacancy distribution in the unit cell of Prussian blue. In certain ancient Prussian blues, the PDF analysis revealed the presence of nanocrystalline ferrihydrite, Fe10O14(OH)2, and also identified the presence of alumina hydrate, Al10O14(OH)2, with a particle size of ca. 15 Å. Paint layers prepared from these ancient pigments subjected to accelerated ageing showed a tendency to turn green, a tendency that was often reported in eighteenth- and nineteenth-century books. The presence of particles of hydrous iron(III) oxides was also observed in a genuine Prussian blue sample obtained from an eighteenth-century polychrome sculpture. [1] Kirby, J.; Saunders, D. The National Gallery Technical Bulletin 2004, 25, 73. [2] Dossie, R. The Handmaid to the Arts; Nourse, J.: London, 1758; Le Pileur d'Apligny, M. Traité des couleurs matérielles et de la manière de colorer relativement aux différents arts et métiers; Saugrain et Lamy: Paris, 1779. [less ▲]

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See detailInvestigation of Epstein-Barr virus (EBV) prevalence in thyroid carcinomas in Tunisia
Trimeche, Mounir; Ziadi, Sonia; Hachana, Mohamed Ridha ULg et al

in Virchows Archiv : An International Journal of Pathology (2007)

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See detailInvestigation of Epstein-Barr virus in Tunisian breast carcinomas
Hachana, Mohamed Ridha ULg; Amara, Khaled; Ziadi, Sonia et al

in Pathology - Research & Practice (2011), 207(11), 695-700

Breast carcinoma is a major cause of death among women, and the potential implication of viruses in its pathogenesis remains worth a hypothesis. The potential role of Epstein-Barr virus (EBV) in its ... [more ▼]

Breast carcinoma is a major cause of death among women, and the potential implication of viruses in its pathogenesis remains worth a hypothesis. The potential role of Epstein-Barr virus (EBV) in its pathogenesis is still a subject of continued discussion and investigation. The aim of this study was to evaluate the prevalence of EBV in sporadic breast cancers in Tunisia, and to determine the clinicopathological characteristics of virus-positive cases. Viral presence has been evaluated by polymerase chain reaction (PCR), in situ hybridization, and immunohistochemistry investigated on tumor tissues and their corresponding normal breast tissues collected from 123 Tunisian women with sporadic breast carcinomas. Viral status in tumors was then correlated with various clinicopathological parameters. Using specific PCR assays, EBV DNA was found in 33 (27%) out of 123 breast carcinoma cases. EBV-encoded small RNAs (EBERs) in situ hybridization was negative in the neoplastic cells, but stomal lymphocytes were positive in 4 cases. Immunohistochemistry for latent membrane protein 1 (LMP1) was negative in all cases. None of the normal breast tissues showed positive results for EBV using PCR, in situ hybridization, and immunohistochemistry. A correlation was found between EBV DNA presence and the negativity of estrogen receptor (P=0.008). However, no significant correlation was found for the other parameters investigated, including patient age, Scarff-Bloom-Richardson (SBR) histological grade, tumor size, and histological node involvement. With regard to survival data, overall and disease-free survivals were shorter in EBV-positive breast carcinoma cases than in EBV-negative ones, but this difference did not reach statistical significance. Our study indicates the presence of EBV DNA in a significant proportion of breast cancer in Tunisia. Further studies are required to elucidate the role of this virus in breast carcinogenesis. [less ▲]

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