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See detailMicroreactor Technology as an Efficient Tool for Multicomponent Reactions
Cukalovic, Ana; Monbaliu, Jean-Christophe ULg; Stevens, Christian

in Orru, Romano; Ruijter, Eelco (Eds.) Topics in Heterocyclic Chemistry: Synthesis of Heterocycles via Multicomponent Reactions I (2010)

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See detailMicroRNA Targeting of CoREST controls polarization of migrating cortical neurons
Volvert; Prévot, Pierre-Paul; Close, Pierre ULg et al

in Cell Reports (2014), 7(4), 1168-83

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See detailMicroRNA targeting of CoREST controls polarization of migrating cortical neurons.
Volvert, M. L.; Prevot, Pierre-Paul ULg; Pirotte, S. et al

Poster (2013)

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See detailMicroRNA-146a is a causative factor and a specific biomarker for peripartum cardiomyopathy
Halkein, Julie ULg; Tabruyn, Sébastien ULg; Haghikia, Arash et al

Poster (2012, April)

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See detailMicroRNA-146a is a therapeutic target and biomarker for peripartum cardiomyopathy.
Halkein, Julie ULg; Tabruyn, Sebastien P.; Ricke-Hoch, Melanie et al

in Journal of Clinical Investigation (2013), 123(5), 2143-54

Peripartum cardiomyopathy (PPCM) is a life-threatening pregnancy-associated cardiomyopathy in previously healthy women. Although PPCM is driven in part by the 16-kDa N-terminal prolactin fragment (16K PRL ... [more ▼]

Peripartum cardiomyopathy (PPCM) is a life-threatening pregnancy-associated cardiomyopathy in previously healthy women. Although PPCM is driven in part by the 16-kDa N-terminal prolactin fragment (16K PRL), the underlying molecular mechanisms are poorly understood. We found that 16K PRL induced microRNA-146a (miR-146a) expression in ECs, which attenuated angiogenesis through downregulation of NRAS. 16K PRL stimulated the release of miR-146a-loaded exosomes from ECs. The exosomes were absorbed by cardiomyocytes, increasing miR-146a levels, which resulted in a subsequent decrease in metabolic activity and decreased expression of Erbb4, Notch1, and Irak1. Mice with cardiomyocyte-restricted Stat3 knockout (CKO mice) exhibited a PPCM-like phenotype and displayed increased cardiac miR-146a expression with coincident downregulation of Erbb4, Nras, Notch1, and Irak1. Blocking miR-146a with locked nucleic acids or antago-miRs attenuated PPCM in CKO mice without interrupting full-length prolactin signaling, as indicated by normal nursing activities. Finally, miR-146a was elevated in the plasma and hearts of PPCM patients, but not in patients with dilated cardiomyopathy. These results demonstrate that miR-146a is a downstream-mediator of 16K PRL that could potentially serve as a biomarker and therapeutic target for PPCM. [less ▲]

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See detailMicroRNA-146a, a downstream effector of 16kDa prolactin, impairs the endothelium-cardiomyocyte cross-talk in peripartum cardiomyopathy
Struman, Ingrid ULg; Halkein, Julie ULg; Tabruyn, Sébastien ULg et al

in FASEB meeting:the Growth Hormone/Prolactin Family in Biology and Disease. (2012)

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See detailmicroRNA-21 Exhibits Anti-Angiogenic Function by Targeting RhoB Expression in Endothelial Cells
Sabatel, Céline; Malvaux, Ludovic; Bovy, Nicolas ULg et al

Poster (2011, February)

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See detailMicroRNA-21 Exhibits Antiangiogenic Function by Targeting RhoB Expression in Endothelial Cells.
Sabatel, Céline; Malvaux, Ludovic ULg; Bovy, Nicolas ULg et al

in PLoS ONE (2011), 6(2), 16979

BACKGROUND: MicroRNAs (miRNAs) are endogenously expressed small non-coding RNAs that regulate gene expression at post-transcriptional level. The recent discovery of the involvement of these RNAs in the ... [more ▼]

BACKGROUND: MicroRNAs (miRNAs) are endogenously expressed small non-coding RNAs that regulate gene expression at post-transcriptional level. The recent discovery of the involvement of these RNAs in the control of angiogenesis renders them very attractive in the development of new approaches for restoring the angiogenic balance. Whereas miRNA-21 has been demonstrated to be highly expressed in endothelial cells, the potential function of this miRNA in angiogenesis has never been investigated. METHODOLOGY/PRINCIPAL FINDINGS: We first observed in endothelial cells a negative regulation of miR-21 expression by serum and bFGF, two pro-angiogenic factors. Then using in vitro angiogenic assays, we observed that miR-21 acts as a negative modulator of angiogenesis. miR-21 overexpression reduced endothelial cell proliferation, migration and the ability of these cells to form tubes whereas miR-21 inhibition using a LNA-anti-miR led to opposite effects. Expression of miR-21 in endothelial cells also led to a reduction in the organization of actin into stress fibers, which may explain the decrease in cell migration. Further mechanistic studies showed that miR-21 targets RhoB, as revealed by a decrease in RhoB expression and activity in miR-21 overexpressing cells. RhoB silencing impairs endothelial cell migration and tubulogenesis, thus providing a possible mechanism for miR-21 to inhibit angiogenesis. Finally, the therapeutic potential of miR-21 as an angiogenesis inhibitor was demonstrated in vivo in a mouse model of choroidal neovascularization. CONCLUSIONS/SIGNIFICANCE: Our results identify miR-21 as a new angiogenesis inhibitor and suggest that inhibition of cell migration and tubulogenesis is mediated through repression of RhoB. [less ▲]

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See detailMicroRNAs in the tumor endothelium: novel controls on the angioregulatory switchboard
Heusschen, Roy ULg; van Gink, Matthijs; Griffioen, Arjan et al

in Biochimica et Biophysica Acta - Reviews on Cancer (2010)

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See detailMicroRNAs tune cerebral cortical neurogenesis.
Volvert, M.-L.; Rogister, F.; Moonen, Gustave ULg et al

in Cell Death & Differentiation (2012), 19(10), 1573-81

MicroRNAs (miRNAs) are non-coding RNAs that promote post-transcriptional silencing of genes involved in a wide range of developmental and pathological processes. It is estimated that most protein-coding ... [more ▼]

MicroRNAs (miRNAs) are non-coding RNAs that promote post-transcriptional silencing of genes involved in a wide range of developmental and pathological processes. It is estimated that most protein-coding genes harbor miRNA recognition sequences in their 3' untranslated region and are thus putative targets. While functions of miRNAs have been extensively characterized in various tissues, their multiple contributions to cerebral cortical development are just beginning to be unveiled. This review aims to outline the evidence collected to date demonstrating a role for miRNAs in cerebral corticogenesis with a particular emphasis on pathways that control the birth and maturation of functional excitatory projection neurons. [less ▲]

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See detailMicrosale interactions between earthworms and microorganisms, a review
Zirbes, Lara ULg; Thonart, Philippe ULg; Haubruge, Eric ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2012), 16(1), 125-131

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See detailMicrosatellite Amplification in Plants: Optimization Procedure of Major PCR Components
Ghaffari, Sana; Hasnaoui, Nejib ULg

in Kantartzi, Stella (Ed.) Methods in Molecular Biology™ series: Microsatllites (2013)

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See detailMicrosatellite mapping of a major determinant of White Heifer Disease: the bovine roan locus
Charlier, Carole ULg; Denys, B.; Belanche, J. I. et al

in Mammalian Genome : Official Journal of the International Mammalian Genome Society (1996), 7

In the Belgian Blue Cattle breed, coat color variation is mainly under the influence of a single autosomal locus, the roan locus, characterized by a pair of codominant alleles: r + (black) and R (white ... [more ▼]

In the Belgian Blue Cattle breed, coat color variation is mainly under the influence of a single autosomal locus, the roan locus, characterized by a pair of codominant alleles: r + (black) and R (white). Heterozygous r + R animals have intermingled black and white hairs, yielding the ``blue'' phenotype typical of the breed. Major interest for the roan locus stems from its pleiotropic effect on fertility, owing to the critical role of the R allele in the determinism of White Heifer Disease. We describe the linkage mapping of the roan locus to bovine Chromosome (Chr) 5, in the interval between microsatellite markers BPI and AGLA293, with an associated lodscore of 11.2. Moreover, we map a candidate gene, the Steel locus coding for the mast cell growth factor, to bovine Chr 5. [less ▲]

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See detailMicrosatellite mapping of a gene affecting horn development in Bos taurus
Georges, Michel ULg; Drinkwater, R.; Lefort, A. et al

in Nature Genetics (1993), 4(2), 206-210

The presence or absence of horns in Bos taurus is thought to be under the genetic control of the autosomal polled locus, characterized by two alleles: P dominant over p, and causing the polled or hornless ... [more ▼]

The presence or absence of horns in Bos taurus is thought to be under the genetic control of the autosomal polled locus, characterized by two alleles: P dominant over p, and causing the polled or hornless phenotype. We have demonstrated genetic linkage between the polled locus and two microsatellite markers, GMPOLL-1 and GMPOLL-2, and have assigned the corresponding linkage group to bovine chromosome 1. This confirms the existence of the postulated polled locus and the hypothesized inheritance pattern. It will allow marker assisted selection for the polledness trait in breeding programs and is a first step towards positional cloning and molecular study of a gene that has been subjected to both natural and artificial selection. [less ▲]

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See detailMicrosatellite mapping of the gene causing weaver disease in cattle will allow the study of an associated QTL
Georges, Michel ULg; Lathrop, M.; Dietz, A. B. et al

in Proceedings of the National Academy of Sciences of the United States of America (1993), 90

A genetic disease in cattle, progressive degenerative myeloencephalopathy (weaver disease), is associated with increased milk production. This association could result from population stratification, from ... [more ▼]

A genetic disease in cattle, progressive degenerative myeloencephalopathy (weaver disease), is associated with increased milk production. This association could result from population stratification, from a pleiotropic effect of a single gene, or from linkage disequilibrium between the gene causing weaver disease and a quantitative trait locus (QTL) for milk production. To test these hypotheses, we performed an extensive linkage study in a bovine pedigree segregating for the weaver condition and identified a microsatellite locus (TGLA116) closely linked to the weaver gene (zmax, 8.15; theta, 0.03). TGLA116 and, by extension, the weaver locus were assigned to bovine synteny group 13. This microsatellite can be used to identify weaver carriers, to select against this genetic defect, and to study the effect of the corresponding chromosomal region on milk production in Brown Swiss and other breeds of cattle. [less ▲]

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See detailMicrosatellite polymorphism in Tunisian pomegranates (Punica granatum L.): Cultivar genotyping and identification
Jbir, Rania; Zehdi, Salwa; Hasnaoui, Nejib ULg et al

in Biochemical Systematics & Ecology (2012), 44

Specific microsatellites (SSRs) markers were used to characterize a set of 32 Tunisian pomegranate (Punica granatum L.) cultivars. Using 13 SSR primers, a total of 40 alleles and 46 genotypes have been ... [more ▼]

Specific microsatellites (SSRs) markers were used to characterize a set of 32 Tunisian pomegranate (Punica granatum L.) cultivars. Using 13 SSR primers, a total of 40 alleles and 46 genotypes have been identified. As a result, data proved that a high level of polymorphism characterizes the Tunisian pomegranate germplasm at the DNA level. The derived Neighbour-joining (NJ) dendrogram constructed using DAS genetic distances exhibited a genetic diversity structured independently from the geographical origin of cultivars and their denomination. This result suggested that a common genetic basis may characterize Tunisian pomegranate cultivars despite their phenotypic divergences. Furthermore, based on the multilocus genotypes a cultivar’s identification key has been established and permitted to unambiguously differentiate between varieties. The obtained results are discussed in term of establishment and management of a national collection of pomegranate varieties, conformity checks, identification of homonyms and synonyms, and screening of the local resources. Furthermore, this microsatellite-based key is a first step towards a marker-assisted identification pomegranate database. [less ▲]

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See detailMicrosatellite polymorphism in Tunisian pomegranates (Punica granatum L.): Cultivar genotyping and identification
Jbir, Rania; Zehdi, Salwa; Hasnaoui, Nejib ULg et al

in Biochemical Systematics & Ecology (2012), 44

Specific microsatellites (SSRs) markers were used to characterize a set of 32 Tunisian pomegranate (Punica granatum L.) cultivars. Using 13 SSR primers, a total of 40 alleles and 46 genotypes have been ... [more ▼]

Specific microsatellites (SSRs) markers were used to characterize a set of 32 Tunisian pomegranate (Punica granatum L.) cultivars. Using 13 SSR primers, a total of 40 alleles and 46 genotypes have been identified. As a result, data proved that a high level of polymorphism characterizes the Tunisian pomegranate germplasm at the DNA level. The derived Neighbour-joining (NJ) dendrogram constructed using DAS genetic distances exhibited a genetic diversity structured independently from the geographical origin of cultivars and their denomination. This result suggested that a common genetic basis may characterize Tunisian pomegranate cultivars despite their phenotypic divergences. Furthermore, based on the multilocus genotypes a cultivar’s identification key has been established and permitted to unambiguously differentiate between varieties. The obtained results are discussed in term of establishment and management of a national collection of pomegranate varieties, conformity checks, identification of homonyms and synonyms, and screening of the local resources. Furthermore, this microsatellite-based key is a first step towards a marker-assisted identification pomegranate database. [less ▲]

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See detailMicrosatellite primers in the Peltigera dolichorhiza complex (lichenized ascomycete, Peltigerales
Magain, Nicolas ULg; Forrest, Laura L.; Sérusiaux, Emmanuël ULg et al

in American Journal of Botany (2010), 97

Detailed reference viewed: 46 (20 ULg)