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Peer Reviewed
See detailIdentification of Three-Element Windkessel Model: Comparison of Time and Frequency Domain Techniques
Pochet, T.; Gérard, Paul ULg; Marnette, J. M. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100(3, May-Jun), 295-301

The problem of the parameter identification of the three-element windkessel model is studied. Minimization by least-square technique--LSQ--in time domain and frequential techniques--FFT--are compared ... [more ▼]

The problem of the parameter identification of the three-element windkessel model is studied. Minimization by least-square technique--LSQ--in time domain and frequential techniques--FFT--are compared. Continuous pressure and flow curves were recorded in the proximal aorta of an open chest dog. Comparison shows very high correlations between the parameter estimations obtained by LSQ and FFT methods. However, systematic differences appear between the calculated values, but do not seem to endanger physiological interpretation of the results. [less ▲]

Detailed reference viewed: 77 (4 ULg)
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See detailIdentification of two antisense transcripts in BLV and their interaction with BLV-encoded microRNAs.
Durkin, Keith ULg; Rosewick, Nicolas; Momont, Mélanie et al

Poster (2014, February)

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See detailIdentification of two FoxP3 genes in rainbow trout (Oncorhynchus mykiss) with differential induction patterns.
Wang, Tiehui; Mira Monte, Milena ULg; Huang, Wenshu et al

in Molecular Immunology (2010), 47(16), 2563-74

FoxP3 is a master transcription factor for the development and function of regulatory T cells in mammals, but little is known about this molecule in fish. Two paralogues of mammalian FoxP3 that share 83.9 ... [more ▼]

FoxP3 is a master transcription factor for the development and function of regulatory T cells in mammals, but little is known about this molecule in fish. Two paralogues of mammalian FoxP3 that share 83.9% identity at the amino acid level have been identified in rainbow trout (Oncorhynchus mykiss). The C-terminal region containing a Zn_C2H2 domain, a leucine zipper-like domain and a forkhead (FH) domain important for dimerization, nuclear translocation, and DNA binding, is well conserved between fish and other vertebrate FoxP3. However, the N-terminal of FoxP3 that is required for FoxP3-mediated repression of transcription is greatly diverged between fish, amphibians and monotreme mammals compared to eutherian mammals, suggesting that FoxP3 in fish, frog and platypus may have a different role to the human and mouse counterpart that defines the Treg cellular lineage and mediates the immune regulatory function. The expression of both trout (t) FoxP3a and tFoxP3b are detectable in all the 14 tissues examined without any significant difference except in muscle in which the expression of tFoxP3a was higher. Both tFoxP3a and tFoxP3b are highly expressed in thymus and in immune related organs including the spleen, kidney, gills and intestine, and are up-regulated by phytohaemagglutinin (PHA) in splenocytes and thymocytes. Whilst the up-regulated tFoxP3b expression induced by PHA was dose-dependent it required a higher PHA concentration to achieve maximal expression relative to tFoxP3a where the highest expression level was seen using 1 mug/ml PHA with higher concentrations having no further effects. In addition, the tFoxP3b expression increased during development from eyed eggs to fry, when it reached a comparable level to that of tFoxP3a. In contrast, tFoxP3a expression was at a high and almost constant level over all of the developmental stages examined. The high level of tFoxP3a expression in early development may be related to the relatively high constitutive level of tFoxP3a expression seen in muscle, perhaps suggesting novel roles of tFoxP3 in fish muscle. The structural and expression analysis suggests that the tFoxP3a and tFoxP3b are subject to differential modulation of expression and may have evolved novel functions. The identification of the two trout FoxP3 paralogues will help to clarify the existence of Treg cells and to dissect the T cell differentiation pathways in fish. [less ▲]

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See detailIdentification of two noncoding antisense transcripts in BLV and their interaction with the BLV encoded miRNAs
Durkin, Keith ULg; Rosewick, Nicolas; Momont, Mélanie et al

Conference (2014, June)

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See detailIdentification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa.
de Chial, Magaly; Ghysels, Bart ULg; Beatson, Scott A. et al

in Microbiology (Reading, England) (2003), 149(Pt 4), 821-31

Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent ... [more ▼]

Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent receptor, FpvA. So far, for P. aeruginosa, three different PVDs, differing in their peptide chain, have been described (types I-III), but only the FpvA receptor for type I is known. Two PVD-producing P. aeruginosa strains, one type II and one type III, were mutagenized by a mini-TnphoA3 transposon. In each case, one mutant unable to grow in the presence of the strong iron chelator ethylenediaminedihydroxyphenylacetic acid (EDDHA) and the cognate PVD was selected. The first mutant, which had an insertion in the pvdE gene, upstream of fpvA, was unable to take up type II PVD and showed resistance to pyocin S3, which is known to use type II FpvA as receptor. The second mutant was unable to take up type III PVD and had the transposon insertion in fpvA. Cosmid libraries of the respective type II and type III PVD wild-type strains were constructed and screened for clones restoring the capacity to grow in the presence of PVD. From the respective complementing genomic fragments, type II and type III fpvA sequences were determined. When in trans, type II and type III fpvA restored PVD production, uptake, growth in the presence of EDDHA and, in the case of type II fpvA, pyocin S3 sensitivity. Complementation of fpvA mutants obtained by allelic exchange was achieved by the presence of cognate fpvA in trans. All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAI has a TAT export sequence at its N-terminal end. [less ▲]

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See detailIdentification of undeclared sources of animal origin in canine dry foods used in dietary elimination trials
Ricci, Rebecca; Granato, A; Vascellari, M et al

in Journal of Animal Physiology & Animal Nutrition (2013), 97

Failure to respond to commercial limited antigen diets can occur in dogs kept on a dietary trial for the diagnosis of adverse food reaction (AFR). The aim of this study was to assess twelve canine dry ... [more ▼]

Failure to respond to commercial limited antigen diets can occur in dogs kept on a dietary trial for the diagnosis of adverse food reaction (AFR). The aim of this study was to assess twelve canine dry limited antigen diets (eleven novel protein diets and one hydrolysed diet) for potential contamination by ingredients of animal origin not mentioned on the label. The validity of the two methods adopted for the detection of such food antigens was also evaluated. Each dietary product was analysed by microscopy analysis using the official method described in Commission Regulation EC 152/2009 with the aim of identifying bone fragments of different zoological classes (mammalian, avian and fish) and by polymerase chain reaction (PCR) for the identification of DNA of animal origin. Discrepancies between the results obtained by PCR and/or microscopy analysis and the ingredients listed on pet food packages were found. Only in two pet foods did the results of both analyses match the ingredients listed on the label. In the remaining ten samples, microscopy detected bone fragments from one or two unpredicted zoological classes, revealing avian fragments in six of ten samples followed by those of fish in five of ten and mammalian fragments in four of ten. In two samples, microscopy analysis identified a contamination that would have otherwise passed unobserved if only PCR had been used. However, PCR confirmed the presence of all the zoological classes detected by microscopy and also identified the DNA of an additional unexpected zoological class in two samples. Dogs might fail to respond to commercial limited antigen diets because such diets are contaminated with potential allergens. Both PCR and microscopy analysis are required to guarantee the absence of undeclared animal sources in pet foods. Before ruling out AFR, a novel protein home-made diet should be considered if the dog is unresponsive to a commercial regimen. [less ▲]

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See detailIdentification of viable myocardium early after acute myocardial infarction using closed-loop arbutamine echocardiography: comparison with positron emission tomography
PIERARD, Luc ULg; MELON, Pierre ULg; LANCELLOTTI, Patrizio ULg et al

in Acta Cardiologica (2001), 56(6), 387-394

Objective -This study sought to evaluate the safety and efficacy of arbutamine echocardiography in identifying contractile reserve and predicting functional improvement early after acute myocardial ... [more ▼]

Objective -This study sought to evaluate the safety and efficacy of arbutamine echocardiography in identifying contractile reserve and predicting functional improvement early after acute myocardial infarction (AMI). Methods and results - Seventeen patients with first AMI underwent arbutamine echocardiography 48 to 96 hours after AMI. Arbutamine was infused by a closed-loop delivery device. The heart rate slope was 4 beats/min and the heart rate target was 20 beats/min above baseline heart rate. A follow-up echocardiogram was obtained one month later. N- 13 ammonia and F- 18 FDG positron emission tomographic (PET) imaging were performed 6 2 days after AMI, before coronary angiography. Mean duration of arbutamine infusion was 6 2 min. There was no complication and there were no major side effects. Myocardial viability was identified with PET in 15 of the 17 patients. Contractile reserve was observed in 10 patients during arbutamine infusion. Functional recovery was identified in 12 patients. Sensitivity, specificity and accuracy of PET and arbutamine echocardiography for predicting functional recovery were 100%, 40%, 76% and 67%, 80%, 84%, respectively. Conclusions - Low-dose arbutamine stress testing is safe early after AMI. Contractile reserve can be rapidly identified by echo cardiography and is specific, but moderately sensitive for predicting reversible dysfunction. [less ▲]

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See detailIdentification of virulotypes and serotypes of enteropathogenic (EPEC) and Shigatoxigenic (STEC) Escherichia coli from healthy cattle at slaughterhouses in Wallonia.
Takaki, Shino; Duprez, Jean-Noël ULg; Fakih, Ibrahim et al

Poster (2016, September)

Escherichia coli producing the attachment-effacement (AE) lesion (EPEC) and/or Shiga toxins (STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the ... [more ▼]

Escherichia coli producing the attachment-effacement (AE) lesion (EPEC) and/or Shiga toxins (STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the intestines of healthy cattle. Besides the O157:H7 serotype, which is the main serotype causing STEC outbreaks in the world EPEC and STEC can belong to dozens of O serogroups. Of them, 9 have been frequently identified worldwide: O5, O26, O103, O104, O111, O118, O121, O145 and O165. The aim of this study is to identify the virulotypes and the O serotypes of EPEC and STEC isolated from healthy cattle at slaughterhouses in Wallonia. A total of 245 faeces (216 <1year-old bulls, 25 cows and 4 heifers) were sampled between April and June 2014 in 2 slaughterhouses in Wallonia and grown overnight at 37°C in Lauryl sulfate Enterobacteriaceae selective broth. The enrichment broths were assayed with an stx1, stx2 (Shiga toxin) and eae (AE lesion) triplex PCR and positive broths were inoculated onto 4 agar media: McConkey’s, Chromagar ES, Chromagar ES with tellurite and Chromagar STEC. Up to ten colonies per plate were picked up, sub-cultured and tested by the colony hybridization assay with gene probes targeting the stx1, stx2 and eae genes. The triplex PCR was again performed on all probe-positive isolates. The PCR-positive E. coli were subsequently assayed with two pentaplex PCR targeting the specific genes coding for the ten O serogroups listed above. Of the 2563 sub-cultured isolates, 744 isolates (29%) from 62 animals (25%) tested positive with the colony hybridization assay. Of them, 687 isolates (92%) from 59 animals were positive with the triplex PCR and the results of both tests were in agreement for 617 isolates (83%). One to 29 isolates per animal were probe- and PCR-positive. The positive isolates grew on Chromagar STEC (379; 55%), on Chromagar ES with tellurite (189; 28%), on Chromagar ES (62; 9%) or on McConkey’s agar (57; 8%). The most frequent virulotypes were eae+ (EPEC: 372 isolates; 54%), eae+stx1+ (AE_STEC: 119 isolates; 17%) and stx2+ (STEC: 118 isolates; 17%). In some animals different virulotypes were identified. The serogrouping with the two pentaplex PCR is in progress. AE-STEC, EPEC and STEC are excreted by 25% of the healthy cattle at slaughterhouses in Wallonia and different virulotypes can be excreted by the same animal. Conversely the methodology followed gives no precise idea of the actual level of excretion since the hybridization and PCR were performed after enrichment in selective broth. Therefore multiple isolates belonging to the same virulotype might represent the same clone. Identification of the serogroups and comparison by Pulsed Field Gel Electrophoresis should help to clarify that point. Quantitative (q)PCR is today the best method to quantify bacterial excretion, but is more expensive. The results of the hybridization and PCR correspond to between 80 and 90%. Though the colony hybridization is still useful for large-scale surveillance it needs radioactive probes for highest sensitivity and is more time-consuming than PCR. Therefore the PCR should be the first routine choice if it can be automatized at large scale. Further steps are the confirmation of the PCR results of the 70 isolates with different hybridization and PCR results and the identification of the serogroups with the two pentaplex PCR and later with PCR for the other serogroups, to compare them with isolates from young diarrhoeic calves and from humans. [less ▲]

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See detailIdentification of VZV ORF9p potential cellular partners that could be important for the viral egress.
Lebrun, Marielle ULg; riva, laura; Rambout, Xavier ULg et al

Poster (2015, July 26)

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate ... [more ▼]

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate of the viral kinase ORF47p and that its ORF47p-dependent phosphorylation is important for the secondary envelopment process. We also have identified an acidic cluster (AC) within the protein that is important for its correct localization in the infected cells and for the interaction with ORF47p. The recombinant VZV expressing ORF9p-ΔAC presents an accumulation of capsids in the perinuclear space. ORF9p seems then to play an important role in several steps of the egress process. In this context, we sought to identify cellular partners of ORF9p that might be important for these functions. We performed a yeast two hybrid screen against the human ORFeome 5.1. and picked out 44 candidates among which 5 proteins playing roles in membrane organization and targeting. We currently are trying to confirm these interactions in infected cells and to assess the role of these interactions for the viral lytic cycle. [less ▲]

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See detailIdentification of walnut husk (Juglans regia L.) volatiles and the behavioural response of the invasiveWalnut Husk Fly, Rhagoletis completa Cresson
Sarles, Landry ULg; Boullis, Antoine ULg; Fassotte, Bérénice ULg et al

in Pest Management Science (2017)

Abstract BACKGROUND: Several European countries are important walnut (Juglans regia L.) producers. However, these countries must contendwith the recent introduction of theWalnut Husk Fly,Rhagoletis ... [more ▼]

Abstract BACKGROUND: Several European countries are important walnut (Juglans regia L.) producers. However, these countries must contendwith the recent introduction of theWalnut Husk Fly,Rhagoletis completa Cresson (Diptera, Tephritidae),which is causing severe economic losses, especially in organic production. Because most Tephritid fruit flies use kairomones in their search for host plants, we hypothesise that this highly specialist species orients toward the volatile blend released by walnut husks. RESULTS:We collected, identified, and quantified the volatile organic chemicals (VOCs) released by walnut husks from themost commonly cultivated variety in France (Franquette). Then, the behavioural response of R. completa toward synthetic odour blends was recorded in dual choice assays conducted in net cages. A total of 26 VOCs were identified, with 𝜶-pinene, 𝜷-pinene, trans-linalool, eugenol, and tetradecane representing the major constituents. In the dual choice assay, male and female R. completa were strongly attracted to synthetic blend that includedmost of the identified husk VOCs. CONCLUSION:When searching for a host plant, R. completa use host fruit kairomones. The potential of these semiochemicals in monitoring andmanagement of this quarantine pest is discussed. [less ▲]

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See detailIdentification of wire rope isolators using the restoring force surface method
Kerschen, Gaëtan ULg; Lenaerts, Vincent; Golinval, Jean-Claude ULg

in International Conference on Structural Systems Identification, Kassel, 2001 (2001)

The restoring force surface method offers an efficient and reliable identification of non-linear single-degree-of-freedom systems. The method may be extended to multidegree-of-freedom systems but by ... [more ▼]

The restoring force surface method offers an efficient and reliable identification of non-linear single-degree-of-freedom systems. The method may be extended to multidegree-of-freedom systems but by loosing the key advantage of the method which lies in the two-dimensional representation for single-degree-of-freedom systems. An experimental application of the restoring force surface method is considered in the present paper. The structure investigated consists of wire rope isolators mounted between a load mass and a base mass. These helical isolators were found to be characterised by a non-linear behaviour. The results obtained are discussed in details and the advantages and drawbacks of the method are underlined. [less ▲]

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See detailIdentification of yield locus parameters of metals using inverse modeling and full field DIC
Lecompte, D.; Cooreman, S.; Sol, H. et al

in Proceedings of the 7th national congress on theoretical and applied mechanics (2006)

The basic principle of the inverse modeling procedure as it is used for parameter identification is the generation of a complex and heterogeneous deformation field that contains as much information as ... [more ▼]

The basic principle of the inverse modeling procedure as it is used for parameter identification is the generation of a complex and heterogeneous deformation field that contains as much information as possible about the parameters to be identified. One way of obtaining such a non-homogeneous deformation is by making the geometry of the specimen less regular. Another possibility is to make the loading conditions more complex. In this paper both options are actually combined by using the concept of a biaxial tensile test on a perforated cruciform specimen. In the present paper, the work hardening of the material is assumed to be isotropic and it is described by a Swift law. The yield locus is modeled by the anisotropic Hill48 criterion. The optimization technique used is a constrained gradient based Newton-type routine, which means that in every iteration step, a sensitivity calculation has to be performed in order to indicate the direction in which the parameters are to be optimized. The functional to be minimized is a least-squares expression of the discrepancy between the measured and the simulated strain fields at a certain load. The numerical routines as well as the identification results of the different parameters, based on simulated strain fields, are discussed. [less ▲]

Detailed reference viewed: 56 (3 ULg)
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See detailIdentification of ‘Textsorten’ in the Late Egyptian Corpus
Gohy, Stéphanie ULg; Martin Leon, Benjamin ULg

in Winand, Jean; Polis, Stéphane (Eds.) Texts, Languages & Information. Technology in Egyptology. Selected papers from the meeting of the Computer Working Group of the International Association of Egyptologists (Informatique & Égyptologie), Liège, 6-8 July 2010 (2010, July 08)

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See detailIdentification On Commercialized Products Of Aflp Markers Able To Discriminate Slow- From Fast-Growing Chicken Strains
Fumiere, Olivier; Dubois, Marc; Gregoire, Dimitrie et al

in Journal of Agricultural and Food Chemistry (2003), 51(5), 1115-1119

The European chicken meat market is characterized by numerous quality marks: “Label de Qualité Wallon” in Belgium, “Label Rouge” in France, denominations of geographical origin, organic agriculture, etc ... [more ▼]

The European chicken meat market is characterized by numerous quality marks: “Label de Qualité Wallon” in Belgium, “Label Rouge” in France, denominations of geographical origin, organic agriculture, etc. Most of those certified productions have specifications requiring the use of slow-growing chicken strains. The amplified fragment length polymorphism (AFLP) technique has been used to search molecular markers able to discriminate slow-growing chicken strains from fast-growing ones and to authenticate certified products. Two pairs of restriction enzymes (EcoRI/MseI and EcoRI/TaqI) and 121 selective primer combinations were tested on individual DNA samples from chicken products essentially in carcass form that were ascribed as belonging to either slow- or fast-growing strains. Within the resulting fingerprints, two fragments were identified as type−strains specific markers. One primer combination gives a band (333 bp) that is specific for slow-growing chickens, and another primer pair generates a band (372 bp) that was found to be characteristic of fast-growing chickens. The two markers were isolated, cloned, and sequenced. The effectiveness and the specificity of the two interesting determinants were assessed on individuals of two well-known strains (ISA 657 and Cobb 500) and on commercialized products coming from various origins. [less ▲]

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See detail"Identification organisationnelle et changement: les logiques identitaires à l'épreuve du New Public Management"
Rondeaux, Giseline ULg

Doctoral thesis (2012)

Depuis près de 30 ans, nombre de pays se sont engagés dans la réforme de leur administration publique, inspirée du New Public Management (NPM), inspirant de multiples travaux et débats sur ses fondements ... [more ▼]

Depuis près de 30 ans, nombre de pays se sont engagés dans la réforme de leur administration publique, inspirée du New Public Management (NPM), inspirant de multiples travaux et débats sur ses fondements et ses déclinaisons, ses avantages et inconvénients, sa pertinence, ses paradoxes ou encore les difficultés liées à sa mise en oeuvre ou à son évaluation. Construit comme une critique des règles de l’administration bureaucratique, ce modèle managérial constitue une profonde remise en cause de celle-ci en termes d’organisation, de management, de GRH et de valeurs fondamentales. Les réformes inspirées du NPM sont aussi à la base d’une redéfinition de l’identité organisationnelle institutionnalisée de l’administration (Du Gay, 1996) : l’organisation s’interroge sur ce qu’elle est, ce qu’elle devient, ce qu’elle veut être, propulsant l’identité organisationnelle au premier plan des préoccupations. Alors que le processus de modernisation de l’administration vise à redéfinir la réponse à la question « qui sommes-nous en tant qu’organisation ? », de quelle manière les membres de celle-ci en font-ils l’expérience? Au travers de l’analyse de trois cas de réforme d’administrations publiques, notre thèse s’intéresse aux dynamiques d’identification à l’oeuvre en regard de la perception des évolutions du contexte par les individus. Nous mettons en évidence le lien qui existe entre les processus d’identification organisationnelle et deux dimensions spécifiques à chaque contexte en évolution : le caractère plus ou moins radical de changement d’identité organisationnelle institutionnalisée que suppose la réforme mise en œuvre, et le degré de maturité de ce processus de réforme, ou en d’autres termes la mesure dans laquelle la réforme est plus ou moins perceptible dans la transformation du contexte même. Au départ des différents éléments relevés dans chacun de nos terrains, notre thèse illustre concrètement comment la mise en œuvre d’un management polyphonique du processus de changement peut contribuer de manière positive à l’identification organisationnelle des membres de ces organisations en évolution. Cette approche mène à réfléchir autrement à la gestion du changement au travers de la prise en compte de cette diversité de positionnements identitaires. Elle conduit en outre à une réflexion plus large sur la mise en œuvre du NPM et sur l’administration publique de demain. [less ▲]

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See detailIdentification par empreintes génétiques des espèces animales entrant dans une chaîne alimentaire
Haezebroeck, Valérie; Renaville, Robert ULg; Bertozzi, Carlo et al

in Cinquième Carrefour des Productions animales. Quels systèmes de Productions Animales pour le 21ème siècle? (2000, January 26)

Detailed reference viewed: 24 (0 ULg)