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See detailMETHis VI. Images fixes / Images en mouvement
Bert, Mathilde ULg; Falque, Ingrid ULg; Hagelstein, Maud ULg

Book published by Presses Universitaires de Liège (2014)

Detailed reference viewed: 28 (4 ULg)
See detailMethIS, 2 : Pratiques du document
Letawe, Céline ULg; Polis, Stéphane ULg; Stasse, Bauduin ULg et al

Book published by CEFAL/Editions de l'ULg (2009)

Detailed reference viewed: 60 (30 ULg)
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See detailA method allowing long term culture and expansion of chicken primordial germ cells
Tonus, Céline ULg; Waroux, Olivier ULg; Grobet, Luc ULg

Conference (2013, March 12)

We developed an original method allowing long term culture (up to 20 month) of undifferentiated chicken PGCs, with good proliferation rates. We use a co-culture system: PGCs are cultivated in cell culture ... [more ▼]

We developed an original method allowing long term culture (up to 20 month) of undifferentiated chicken PGCs, with good proliferation rates. We use a co-culture system: PGCs are cultivated in cell culture inserts, in the presence of irradiated Buffalo Rat Liver cells. According to our own experience, physical separation of PGCs and feeder cells limits the differentiation of PGCs. Our PGCs lines keep their original phenotype including their gonadal colonization ability. We also developed a novel cryopreservation method based on vitrification, in complement of a slow rate freezing. [less ▲]

Detailed reference viewed: 15 (3 ULg)
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See detailA method and an apparatus for separating a first amount of liquid
Vandewalle, Nicolas ULg; Gilet, Tristan; Jérôme, Christine ULg et al

Patent (2010)

The invention relates to a method for separating a first amount of liquid into a plurality of second amounts of liquid, the method comprising the steps of bringing the first amount of liquid (210) into ... [more ▼]

The invention relates to a method for separating a first amount of liquid into a plurality of second amounts of liquid, the method comprising the steps of bringing the first amount of liquid (210) into contact with at least one first fibre (110) being arranged substantially vertical, allowing the first amount of liquid to drain of the first fibre, thereby forming a coating film on the fibre, allowing the coating film to decompose, thereby forming a plurality of second amounts of liquid (220), and allowing the second amounts of liquid to drain of the first fibre and being captured at a crossing point (130) being constituted by the first fibre and at least one second fibre (120) being arranged substantially perpendicular to the first fibre and touching the first fibre. Furthermore, the invention relates to an apparatus for separating a first amount of liquid into a plurality of second amounts of liquid. [less ▲]

Detailed reference viewed: 27 (13 ULg)
See detailMethod and kit for the measurement of neutrophil cell activation
Serteyn, Didier ULg; Deby-Dupont, Ginette; Franck, Thierry ULg et al

Patent (2006)

The present invention is related to methods and kits (or devices) for the measurement of equine myeloperoxidase (MPO), a specific enzyme of equine neutrophils, either in total [first method], or ... [more ▼]

The present invention is related to methods and kits (or devices) for the measurement of equine myeloperoxidase (MPO), a specific enzyme of equine neutrophils, either in total [first method], or specifically in its active form [second method]. Said methods and kits (or devices), used independently or in combination, find improved applications in the veterinary field and can be adapted for application in human health care. The concept of the second method is applicable to any other enzyme. [less ▲]

Detailed reference viewed: 21 (1 ULg)
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See detailA method based on multiple adaptive windows to improve the determination of disparity maps
Demoulin, Cédric; Van Droogenbroeck, Marc ULg

in ProRISC/IEEE Workshop on Circuit, Systems and Signal Processing (2005, November)

The correspondence problem is one of main topics in stereo vision that, despites being studied for many years, is still in progress. In this paper, we present a new method that computes the disparity map ... [more ▼]

The correspondence problem is one of main topics in stereo vision that, despites being studied for many years, is still in progress. In this paper, we present a new method that computes the disparity map. Our method is local (i.e. only information gathered in the close neighborhood is used) and is based on image statistics. More specifically it combines multiple adaptive windows and local statistical measures in order to optimize the quality of the computation of disparity maps. As the size of windows is critical for local methods, we propose an algorithm that modifies the size of windows surrounding the pixel of interest to capture enough information in regions with low texture energy. This process is based on statistical measures (mean and horizontal/vertical average deviation) taken in the windows of the original stereo images. In the last section we show that our method performs very well compared to other existing local methods. [less ▲]

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See detailMethod developement and applications of capillary electrophoresis in drug analysis
Crommen, Jacques ULg; Bechet, I.; Paques, Ph. et al

Conference (1993)

Detailed reference viewed: 7 (1 ULg)
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See detailMethod Development Strategies for the Enantioseparation of Drugs by Capillary Electrophoresis Using Cyclodextrins as Chiral Additives
Fillet, Marianne ULg; Hubert, Philippe ULg; Crommen, Jacques ULg

in Electrophoresis (1998), 19(16-17), 2834-40

General strategies for the development of capillary electrophoretic methods for the enantiomeric separation of basic, acidic or neutral drugs were developed. For all kinds of compounds, the use of a ... [more ▼]

General strategies for the development of capillary electrophoretic methods for the enantiomeric separation of basic, acidic or neutral drugs were developed. For all kinds of compounds, the use of a buffer made of 100 mM phosphoric acid adjusted to pH 3 with triethanolamine and containing anionic and/or uncharged cyclodextrin (CD) derivatives as chiral selectors was recommended. Two different optimization schemes depending on the acidic or basic character of the analytes, were elaborated. For most basic compounds present in cationic form at pH 3, enantiomeric separation could be achieved in the normal polarity mode. Different beta-cyclodextrin derivatives were first tested at a given concentration. Five derivatives were found to be particularly useful for enantioseparations in capillary electrophoresis (CE): the anionic carboxymethyl-beta-CD (CMCD) and sulfobutyl-beta-CD (SBCD) and the neutral dimethyl-beta-CD (DMCD), trimethyl-beta-CD (TMCD) and hydroxypropyl-beta-CD (HPCD). After selection of the most suitable CD, its concentration was optimized with respect to chiral resolution. If necessary, a further improvement in resolution could often be obtained for the enantiomers of cationic solutes by increasing the buffer pH from 3 to 5 using CMCD as chiral additive. Another possible alternative for enhancement in chiral resolution was the addition of metharlol or cyclohexanol to the buffer. For acidic drugs, essentially present in uncharged form at pH 3, and for neutral solutes, anionic CD derivatives such as SBCD or CMCD were first tested at a given concentration in the reversed polarity mode. Dual systems, based on the simultaneous addition of a charged CD (SBCD or CMCD) and a neutral CD (TMCD or DMCD), could then be investigated for resolution improvement. After optimization of the CD concentrations, the use of dual systems with CMCD at pH 5 could also be tested if necessary, especially for very weak acidic and neutral drugs. By applying these optimization strategies, 48 of the 50 drugs examined as model compounds could be fully enantioseparated by CE in short analysis times (usually less than 10 min). [less ▲]

Detailed reference viewed: 17 (5 ULg)
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See detailMethod development strategies for the rapid enantioseparation of drugs by capillary electrophoresis using cyclodextrins as chiral additives
Fillet, Marianne ULg; Fotsing, L.; Piette, V. et al

in Journal de Pharmacie de Belgique (1998), 53

Detailed reference viewed: 3 (0 ULg)
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See detailA Method for Applying LOTOS at an Early Design Stage and Its Application to the ISO Transport Protocol
Leduc, Guy ULg

in The OSI95 Transport Service with Multimedia Support (1994)

We propose a method applicable to the design of large and abstract LOTOS specifications. More precisely, this method explains how to generate a constrain-oriented specification which is an adequate ... [more ▼]

We propose a method applicable to the design of large and abstract LOTOS specifications. More precisely, this method explains how to generate a constrain-oriented specification which is an adequate abstract and modular starting point of a complex design process leading to implementation. It is illustrated on a substantial part of the ISO Transport Protocol class 4 which is considered as a complex and stable case study. Having proved the feasibility of the method on this protocol, it was then used to specify (parts of) the new transport protocol TPX of OSI95. [less ▲]

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See detailMethod for co-purification of equine neutrophil elastase and myeloperoxidase from a limited blood volume.
de la Rebière de Pouyade, Geoffroy ULg; Serteyn, Didier ULg; Deby, Ginette ULg et al

in Research in Veterinary Science (2009)

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities ... [more ▼]

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities. We describe the isolation of both enzymes from a same limited blood volume. Both MPO and elastase were extracted by crushing PMN isolated by centrifugation on a percoll-gradient from a 460ml blood collection. MPO and elastase were separated by an ionic exchange chromatography phase and further purified by gel filtration chromatography on Superdex 200 and 75, respectively. Enzymes were identified in the collected fractions by specific enzymatic assays. The final purity was verified by electrophoresis. Specific activity was improved to 19.92 and 34.3x for elastase (final yield: 340mug) and MPO (final yield: 130mug), respectively, during the procedure. Results show the possibility of isolating both enzymes from the same blood sample with a sufficient yield and purity for future studies on their implication and interaction during inflammatory diseases. [less ▲]

Detailed reference viewed: 37 (14 ULg)
See detailMethod for controlled radical polymerisation
Jérôme, Robert ULg; Creutz, Serge; Dubois, Philippe et al

Patent (1998)

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general ... [more ▼]

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general formula (I) is used in which the substituents (Z)m, (R)n and Y can have different values. [less ▲]

Detailed reference viewed: 5 (2 ULg)
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See detailMethod for controlled radical polymerization
Jérôme, Robert ULg; Creutz, Serge; Dubois, Philippe et al

Patent (1998)

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general ... [more ▼]

The invention concerns a method for making a polymer or a copolymer by radical polymerisation of at least one unsaturate monomer in the presence of a stabiliser whereby a stabiliser complying with general formula (I) is used in which the substituents (Z)m, (R)n and Y can have different values. [less ▲]

Detailed reference viewed: 21 (7 ULg)