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See detailHumnétriai… Un rite mis en images chez Philostrate
Pironti, Gabriella; Pirenne-Delforge, Vinciane ULg

in Calame, Claude; Ellinger, Pierre (Eds.) Du récit au rituel par la forme esthétique. Poèmes, images et pragmatique cultuelle en Grèce ancienne (2017)

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See detailHumoral and Cell-Mediated Immune Responses of Beef and Dairy Cattle Experimentally Infested with Psoroptes Ovis
Lonneux, J. F.; Nguyen, T. Q.; Hollanders, W. et al

in American Journal of Veterinary Research (1998), 59(5), 583-7

OBJECTIVE: To compare cellular and humoral immune responses of beef (Belgian White and Blue [BWB]) and dairy (Friesian-Holstein [FH]) cattle to Psoroptes ovis infestation and to determine whether P ovis ... [more ▼]

OBJECTIVE: To compare cellular and humoral immune responses of beef (Belgian White and Blue [BWB]) and dairy (Friesian-Holstein [FH]) cattle to Psoroptes ovis infestation and to determine whether P ovis infestation impaired immune responses to infectious bovine rhinotracheitis virus (IBR) vaccine or an immunogenic protein (keyhole-limpet hemocyanin [KLH]). ANIMALS: 19 BWB and 6 FH 1-year-old calves. PROCEDURE: 2 trials were performed. In each trial, 7 (trial 1) or 6 (trial 2) BWB calves and 3 FH calves were experimentally infested with P ovis and 3 BWB calves were maintained as uninfested controls. Animals were inoculated with KLH and IBR virus vaccine twice; 3 BWB calves in each trial were treated with ivermectin. Serum antibody responses to KLH, IBR virus, and P ovis were measured by use of ELISA. A lymphocyte transformation assay was used to determine nonspecific responses to 3 mitogens and specific lymphocyte reactivity to P ovis antigen. RESULTS: In each trial, 3 BWB and 3 FH calves developed clinical signs of psoroptic mange and mites could be recovered. Infested and control animals developed similar antibody titers to KLH and IBR virus. Antibodies to P ovis were detected early in some infested calves, and this was correlated with a marked cell-mediated immune response. Lymphocyte responsiveness to the 3 mitogens was not significantly different among groups. CONCLUSIONS: In these calves, infestation with P ovis induced a marked humoral and cell-mediated immune response. Immunosuppression was not evident. [less ▲]

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See detailHumoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs.
Mignon, Bernard ULg; Leclipteux, T.; Focant, Charles ULg et al

in Medical Mycology (1999), 37(2), 123-129

In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a ... [more ▼]

In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a crude exo-antigen and a 31.5 kDa purified keratinase were evaluated in 12 M. canis experimentally infected guinea pigs. Humoral and cellular responses were assessed by ELISA from days 0 to 56 postinfection (PI) and by measurement of delayed-type hypersensitivity (DTH) responses on days 14 and 57 PI, respectively. Additionally, immunohistochemical staining was performed and demonstrated that the keratinase was produced in infected guinea pig skin, as previously reported in cats. Despite a marked interindividual variation, all the guinea pigs produced specific IgG to the crude exo-antigen from day 21 PI onwards, but no anti-keratinase IgG was detected. Strongly positive DTH responses to the exo-antigen were observed on both dates, whereas the keratinase elicited no and weak DTH on days 14 and 57 PI, respectively. These results are in agreement with those previously described for naturally infected cats, and indicate that the 31.5 kDa keratinase is not a major antigen in M. canis infection. [less ▲]

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See detailHumoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Brouta, Frédéric; Descamps, Frédéric; Vermout, Sandy et al

in Medical Mycology (2003), 41(6), 495-501

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a ... [more ▼]

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis. [less ▲]

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See detailHumoral antibody response to bovine leukemia virus infection in cattle and sheep.
Bex, Françoise; Bruck, Claudine; Mammerickx, Marc et al

in Cancer Research (1979), 39(3), 1118-1123

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See detailHumoral Immune Response in Calves to Single-Dose, Trickle and Challenge Infections with Fasciola Hepatica
Bossaert, K.; Farnir, Frédéric ULg; Leclipteux, Thierry ULg et al

in Veterinary Parasitology (2000), 87(2-3), 103-23

In cattle experimentally infected with Fasciola hepatica, parasite specific IgG1 and IgG2 responses were studied. Additionally parasite specific IgE production was assessed by the Passive Cutaneous ... [more ▼]

In cattle experimentally infected with Fasciola hepatica, parasite specific IgG1 and IgG2 responses were studied. Additionally parasite specific IgE production was assessed by the Passive Cutaneous Anaphylaxis reaction. The primary infection was administered either as a single-dose or as a trickle infection over a 4-week period. Animals were challenged 4 months later. Titres of IgG1 and IgG2 against excretory-secretory parasite products (FhESAg), and against a whole-worm extract (FhSomAg) were measured by enzyme-linked immunosorbent assay (ELISA) in relation to weight gain, serum hepatic enzyme levels, and fluke infection rate. At necropsy, the mean number of flukes recovered was similar in both infected groups. The two ELISAs specific for bovine IgG1 showed analogous sensitivity and specificity (92% and 94%). Cross-reactivity was observed towards Echinococcus granulosus, Cysticercus tenuicollis, and C. ovis but not towards C. bovis, Cooperia spp., and Ostertagia spp. FhESAg gave rise to apparently more stable specific IgG1 titres as compared to FhSomAg. Mean IgG1 titres were significantly higher in the single-dose-infected group than in the trickle-infected group during the early migratory phase of the infection (week 2 to week 4 (FhSomAg) or week 6 (FhESAg)). IgG2 values were consistently lower than IgG1 levels. The kinetic response of both isotypes yielded a similar pattern. Specific IgE antibodies were detected in cattle of both infected groups from week 2 post-primary infection (PPI) onwards. The mean serum glutamate dehydrogenase (GLDH) and gamma-glutamyl transferase (gammaGT) activities were significantly higher in the single-dose-infected group for 3 weeks around peak levels (12-14 weeks PPI and 14-16 weeks PPI for GLDH and gammaGT respectively). Western blotting revealed a major antigenic fraction in FhESAg (26-30 kDa) recognized specifically by sera from F. hepatica infected calves as early as 6-8 weeks PPI. Experimental challenge caused no statistically significant modification of any parameter (IgG1 and IgG2 titres, enzymatic activities, immunoblotting) used to monitor the course of the infection. No correlation was found between fluke size and number, and antibody titres, suggesting that IgG1 production has little protective effect against F. hepatica infection. [less ▲]

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See detailL'humour à la croisée des rapports interculturels
Defays, Jean-Marc ULg

in Cahiers francophones d'Europe Centre-Orientale (1995), I(5/6), 57-66

Detailed reference viewed: 117 (5 ULg)
See detailL'humour de Madame Bovary
Saint-Amand, Denis ULg

Conference given outside the academic context (2013)

Detailed reference viewed: 393 (5 ULg)
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See detail« L’humour toujours l’humour (suite et fin) »
Demoulin, Laurent ULg; Aron, Paul

in Carnet et les Instants (2011), 165

Suite et fin de l'exploration de l'humour dans la littérature belge.

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See detail« L’humour toujours l’humour (suite) »
Demoulin, Laurent ULg

in Carnet et les Instants (2010), 164

Etude succincte de l'absurde chez quelques écrivains belges des XXe et XXIe siècles.

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See detailL'humour: le texte au banc d'essai
Defays, Jean-Marc ULg

Conference (1990, September)

Detailed reference viewed: 53 (3 ULg)
See detailHumTec-EET – Modellierung zukünftiger Energieversorgungsstrukturen
Frenzel, P; Kopriwa, Nicole; Hillerbrand, Rafaela et al

Conference (2010)

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See detailHunger and Food Concerns in History: The Increasing Importance of Taste
Von Hoffmann, Viktoria ULg

Conference given outside the academic context (2014)

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See detailHunger Art and the Sensorium
Delville, Michel ULg

Conference (2017, May 10)

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See detailThe hunt for original microbial enzymes: an initiatory review on the construction and functional screening of (meta)genomic libraries
Martin, Marjolaine ULg; Vandenbol, Micheline ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2016), 20(4),

Introduction. Discovering novel enzymes is of interest in both applied and basic science. Microbial enzymes, which are incredibly diverse and easy to produce, are increasingly sought by diverse approaches ... [more ▼]

Introduction. Discovering novel enzymes is of interest in both applied and basic science. Microbial enzymes, which are incredibly diverse and easy to produce, are increasingly sought by diverse approaches. Literature. This review first distinguishes culture-based from culture-independent methods, detailing within each group the advantages and drawbacks of sequence- and function-based methods. It then discusses the main factors affecting the success of endeavors to identify novel enzymes through construction and functional screening of genomic or metagenomic libraries: the sampled environment, how DNA is extracted and processed, the vector used (plasmid, cosmid, fosmid, BAC, or shuttle vector), the host cell chosen from the available prokaryotic and eukaryotic ones and the main screening steps. Conclusions. Library construction and screening can be tricky and requires expertise. Combining different strategies, such as working with cultivable and non-cultivable organisms, using sequence- and function-based approaches, or performing multihost screenings, is probably the best way to identify novel and diverse enzymes from an environmental sample. [less ▲]

Detailed reference viewed: 49 (4 ULg)