Pieter Brueghel le Jeune a-t-il pu voir les tableaux de son père? Réflexions méthodologiques et critiques

in Van den Brink, Peter (Ed.) L'entreprise Brueghel (2001)

The pig as an experimental and teaching model in interventionnal radiology

Poster (1996)

Pig tissues express a catalytically inefficient 25-kDa thiamine triphosphatase: Insight in the catalytic mechanisms of this enzyme

in Biochimica et Biophysica Acta - General Subjects (2005), 1725(1), 93-102

Thiamine triphosphate (ThTP) is found in most organisms and may be an intracellular signal molecule produced in response to stress. We have recently cloned the cDNA coding for a highly specific mammalian ... [more ▼]

Thiamine triphosphate (ThTP) is found in most organisms and may be an intracellular signal molecule produced in response to stress. We have recently cloned the cDNA coding for a highly specific mammalian 25-kDa thiamine triphosphatase. The enzyme was active in all mammalian species studied except pig, although the corresponding mRNA was present. In order to determine whether the very low ThTPase activity in pig tissues is due to the absence of the protein or to a lack of catalytic efficiency, we expressed human and pig ThTPase in E. coli as GST fusion proteins. The purified recombinant pig GST-ThTPase was found to be 2-3 orders of magnitude less active than human GST-ThTPase. Using site-directed mutagenesis, we show that, in particular, the change of Glu85 to lysine is responsible for decreased solubility and catalytic activity of the pig enzyme. Immunohistochemical studies revealed a distribution of the protein in pig brain very similar to the one reported in rodent brain. Thus, our results suggest that a 25-kDa protein homologous to hThTPase but practically devoid of enzyme activity is expressed in pig tissues. This raises the possibility that this protein may play a physiological role other than ThTP hydrolysis. [less ▲]

Pigeon Mara

in Aubenas, Jacqueline (Ed.) Dic Doc: Dictionnaire des Documentaristes belges (1999)

Pigments from the Middle Palaeolithic levels of Es-Skhul (Mount Carmel, Israel)

in Journal of Archaeological Science (2010), 37

Discovery of pigments at Middle Palaeolithic sites is of interest in the context of the ongoing debate about the tempo and mode of the emergence of modern human behaviour. Here we analyse four previously ... [more ▼]

Discovery of pigments at Middle Palaeolithic sites is of interest in the context of the ongoing debate about the tempo and mode of the emergence of modern human behaviour. Here we analyse four previously undescribed fragments of pigmental material from Es-Skhul shelter, layer B, Israel, McCown excavations, identified at the Department of Palaeontology, Natural History Museum, London. One of them is still partially embedded in the hard breccia characteristic of this layer. Inspection of breccia fragments from layer B has led to the identification of small pieces of red and orange pigmental material still enclosed in large clasts, further corroborating the attribution of the larger pieces analysed in this study to layer B. The four objects are studied using optical microscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX), X-ray micro-diffraction (m-XRD), Particle-Induced X-Ray Emission Spectrometry (PIXE), and Proton-Induced Gamma-ray Emission (PIGE). The specimens display various hues of yellow, orange, red, and one of them presents a gradual variation of shade from yellow to dark orange. SEM/EDX analysis identifies two specimens (Skhul 1 and 2) as being composed of iron-rich calcium phosphate, the third (Skhul 3) of potassium-rich clay with titanium-rich iron oxide inclusions, and the fourth (Skhul 4) of pure iron oxide crystals. TEM/ EDX and m-XRD analysis demonstrate that three pieces (Skhul 1, 2 and 4) were heated to at least 300 C, a process that has partially or completely dehydrated goethite into haematite and changed their pristine yellow colour into orange or red. Skhul 3 shows no sign of heating, suggesting that its haematite content has a geological origin. The different mineral composition of the pieces suggests that they must come from a variety of sources. This implies that the associated collection strategies included the selection of materials that differed not only with respect to colour but also with respect to other physical and chemical properties. Although no formal proof exists that these lumps of pigmental material were deliberately heated, results obtained are consistent with this explanation. [less ▲]

Piles à combustible : situation actuelle et perspectives

Conference (2010, May 27)

Pilgrimage out of Dispossession

in Commonwealth : Essays and Studies (1994), 17(1), 100-106

Goodison's poems testify to her proud self-assertion, both as woman and as a descendant of African slaves.

Pili genes pattern in Group B streptococci from newborn infections and pregnant women in Europe (DEVANI Project)

Poster (2011, September)

Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI ... [more ▼]

Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI (Design of a Vaccine Against Neonatal Infection) project. Methods GBS isolates from pregnant women (PW) and cases of newborn infection (NI) were collected in 8 European countries (Belgium, Bulgaria, Czech Republic, Denmark, Germany, Italy, Spain, United Kingdom) during 2009/10 under the auspices of DEVANI. Total no. of strains examined was 1078 and 192 from PW and NI, respectively. Isolates were screened by multiplex PCR and FACS analysis to evaluate respectively gene presence and surface-exposure of pili. Results The most common gene patterns found were PI-2a alone, PI 1+2a and PI 1+2b, while the PI-2b gene alone was very rare. The most prominent result was that a majority of isolates from NI carried the PI-1+2b gene pattern, while the most common pattern among PW was PI-1+2a. Most of analyzed strains express at least one pilus on their surface. Conclusions All isolates contained at least one gene coding for pili. When present pili 2a and 2b were highly surface exposed. [less ▲]

Le pilomatricome unique ou multifocal et son pendant malin.

in Dermatologie Actualité (2010), 121

Pilootproject : Ontslagmanagement in Belgische Psyciatrische Ziekenhuizen. [Pilot study : discharge management in Belgian psychiatric hospitals]. Summarizing report, 2000

Report (2000)