Immunodetection of RNA on ultra-thin sections incubated with polyadenylate nucleotidyl transferase.
in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1993), 41(5), 657-65
A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and ... [more ▼]
A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and biotinylated ATP. The labeled nucleotides bound to RNA at the surface of the ultra-thin sections were than visualized by an indirect immunogold labeling technique. The resulting labeling pattern was dependent on the presence of divalent cations in the PnT medium. The method revealed with great precision the specific RNA-containing structures within Ehrlich tumor cells. The method is applicable to Epon sections. However, the labeling intensity varies according to the fixation used. Best results were obtained on acetylated cell sections. The method can be combined with EDTA regressive staining. The in situ PnT method provides a very useful tool for pinpointing the precise location of RNA within biological material at the ultrastructural level. [less ▲]Detailed reference viewed: 9 (0 ULg)
Immunodetection of the metastasis-associated laminin receptor in human breast cancer cells obtained by fine-needle aspiration biopsy.
Castronovo, Vincenzo ; ; et al
in American Journal of Pathology (1990), 137(6), 1373-81
Fine-needle aspiration biopsy of the breast is a very useful technique for the evaluation of a suspect lesion before surgical removal. Increased expression of the 67-kd laminin receptor has been ... [more ▼]
Fine-needle aspiration biopsy of the breast is a very useful technique for the evaluation of a suspect lesion before surgical removal. Increased expression of the 67-kd laminin receptor has been associated with the metastatic phenotype of cancer cells, particularly in colon and breast cancers. In this study, the expression of laminin receptor was evaluated using the immunoperoxidase technique in 81 breast aspirates (26 benign and 55 neoplastic lesions). Cells obtained from benign samples exhibited a low level of laminin receptor antigen detected by affinity-purified antibody raised against a cDNA-derived laminin receptor peptide. In contrast, 71% of smears obtained from malignant breast lesions contained cells that were strongly stained by the antibody. Heterogeneous expression of the laminin receptor was noted in both breast aspirates and fixed tissue specimens. These data suggest that the immunodetection of laminin receptor in cells obtained by fine-needle aspiration of breast lesions could be a valuable adjunct in the prognostic evaluation of breast lesions. [less ▲]Detailed reference viewed: 9 (1 ULg)
Immunoelectron microscope detection of newly synthetized RNA in isolated nucleoli of Ehrlich tumor cells
Thiry, Marc ; ;
Poster (1995)Detailed reference viewed: 5 (0 ULg)
Immunoelectron microscope detection of nucleic acids in perichromatin granules of HeLa cell nuclei
Thiry, Marc ;
Poster (2005)Detailed reference viewed: 7 (1 ULg)
Immunoelectron microscope detection of replicated DNA by monoclonal anti-bromodeoxyuridine antibody on ultrathin sections
in Biology of the Cell (1988), 63Detailed reference viewed: 5 (1 ULg)
Immunoelectron microscope localization of bromodeoxyuridine incorporated into DNA of Ehrlich tumor cell nucleoli.
in Experimental Cell Research (1988), 179(1), 204-13
The distribution of DNA within the nucleolus of Ehrlich tumor cells has been investigated by means of a recent immunocytochemical approach involving an electron microscopic detection of incorporated 5 ... [more ▼]
The distribution of DNA within the nucleolus of Ehrlich tumor cells has been investigated by means of a recent immunocytochemical approach involving an electron microscopic detection of incorporated 5-bromodeoxyuridine (BUdR) into DNA by an anti-BUdR monoclonal antibody. An immunogold method has been performed on ultrathin sections of cells embedded in Lowicryl K4M. In the nucleolus, gold particles are essentially found over the perinucleolar chromatin adn over its intranucleolar invaginations which are connected with the fibrillar centers. In addition, a few gold particles are also observed in the fibrillar centers, preferentially toward their peripheral regions. In contrast, the dense fibrillar component is completely devoid of labeling. The results are discussed in the context of other recent findings concerning the functional organization of the nucleolus. [less ▲]Detailed reference viewed: 9 (0 ULg)
Immunoelectron microscope localization of DNase I sensitive sequences within the cell nucleus
in Cell Biology International Reports (1990), 14Detailed reference viewed: 8 (0 ULg)
Immunoelectron microscopic study of nucleolar DNA during mitosis in Ehrlich tumor cells.
Thiry, Marc ; ; Goessens, Guy
in European Journal of Cell Biology (1988), 47(2), 346-57
In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU ... [more ▼]
In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU) monoclonal antibody have been applied. In both cases, the immunogold labeling has been performed on ultrathin sections of cells embedded either in Lowicryl K4M or in Epon, respectively. Identical results are observed with both immunocytochemical approaches. In the interphase nucleolus, besides the labeling of the perinucleolar chromatin shell and of its intranucleolar invaginations which penetrate into the nucleolar body and often terminate at the fibrillar centers, a few gold particles are also preferentially found towards the peripheral region of the fibrillar centers. In contrast, the dense fibrillar component and the granular component are never labeled. During mitosis, the fibrillar centers persist at the chromosomal nucleolus organizing regions (NOR's) and can be selectively stained by the silver method. However, these metaphase fibrillar centers are no longer decorated by the DNA- or BrdU antibodies. These results indicate that until the end of prophase, rRNA genes are present inside the fibrillar center material, disappear during metaphase and reappear in reconstituting nucleoli during telophase. Thus, fibrillar centers appear to represent structures sui generis, which are populated by rRNA genes only when the nucleolus is functionally active. In segregated nucleoli after actinomycin D treatment, the DNA labeling is exclusively restricted to the perinucleolar chromatin blocks. These findings also suggest that the DNA content of the fibrillar center material varies according to the rRNA transcription level of the cells. The results are discussed in the light of the present knowledge of the functional organization of the nucleolus. [less ▲]Detailed reference viewed: 9 (3 ULg)
Immunoendocrinology in Health and Disease
Geenen, Vincent ;
Book published by Marcel Dekker (2004)
L'objectif de cet ouvrage est d'illustrer l'importance de l'immunoendocrinologie pour la compréhension de la pathogénie et le traitement des endocrinopathies auto-immunes, et des maladies inflammatoires ... [more ▼]
L'objectif de cet ouvrage est d'illustrer l'importance de l'immunoendocrinologie pour la compréhension de la pathogénie et le traitement des endocrinopathies auto-immunes, et des maladies inflammatoires, infectieuses, allergiques et néoplasiques, de même que le processus d'immunosénescence. Des experts internationaux de chaque domaine ont rédigé des chapitres à propos de maladies chroniques qui ont un impact majeur tant émotionnel qu'économique sur la société humaine. [less ▲]Detailed reference viewed: 56 (1 ULg)
L'immunoenzymétrie améliore les performances diagnostiques du dosage de la CK-MB chez le patient atteint d'infarctus du myocarde
Chapelle, Jean-Paul ; ; El Allaf, Dia et al
Poster (1987, May)Detailed reference viewed: 8 (0 ULg)
Immunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix.
; Foidart, Jean-Michel ; et al
in Cancer Research (1982), 42(6), 2384-91
In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has ... [more ▼]
In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has many of the biochemical characteristics of normal cartilage. With the use of immunofluorescence techniques, tissue slices and chondrocytes in culture were tested for the presence of collagen types I and II, cartilage-characteristic proteoglycan, and fibronectin. Both normal and tumor matrix contained type II collagen and cartilage proteoglycan, but only the tumor matrix contained fibronectin. In culture, tumor-derived chondrocytes continued to accumulate fibronectin in their matrix, even after deposition of type II collagen and proteoglycans, while normal chondrocytes did not. When the attachment characteristics of both types of chondrocytes were compared, tumor chondrocytes required fibronectin for attachment, while normal chondrocytes used another attachment factor that had been identified previously as chondronectin. These studies suggest that, although biochemically similar to normal chondrocytes, tumor chondrocytes are no longer able to express the regulatory mechanisms for fibronectin accumulation. [less ▲]Detailed reference viewed: 11 (0 ULg)
Immunofluorescence study of the antigens of the basement membrane and the peritumoral stroma in human colonic adenocarcinomas.
; ; Foidart, Jean-Michel
in Annals of the New York Academy of Sciences (1983), 420
Twenty-three colonic adenocarcinomas were studied by immunofluorescence with antisera against components of the basement membrane (type IV collagen, laminin, and heparan sulfate-rich proteoglycan) as well ... [more ▼]
Twenty-three colonic adenocarcinomas were studied by immunofluorescence with antisera against components of the basement membrane (type IV collagen, laminin, and heparan sulfate-rich proteoglycan) as well as antisera against antigens of the connective tissue (type III collagen, fibronectin, and hyaluronectin). Marked alterations of the basement membranes were consistently observed on staining with each one of the first three antisera. In contrast, staining of the normal components of connective tissue was in most cases as intense in tumors as in normal colonic mucosa. Hyaluronectin, a marker of peritumoral stroma, was found to be present in 13 out of 16 tumors studied. In six metastatic lymph nodes, tumor foci were sometimes surrounded by antigens of the basement membrane. But these antigens were never found in the cytoplasm of cancer cells. [less ▲]Detailed reference viewed: 8 (0 ULg)
Immunofluorescent localization of type IV collagen and laminin during endochondral bone differentiation and regulation by pituitary growth hormone
Foidart, Jean-Michel ;
in Developmental Biology (1980), 75(1), 130-6Detailed reference viewed: 5 (0 ULg)
Immunogenetics as a tool to assess Hantavirus risk in wild rodents
; ; Pagès, Marie et al
Conference (2011, July)
Immunogenetics, the analysis of genetic polymorphisms in specific recognition and immune regulation, is at the core of the study of host-parasite coevolution. We present here a synthesis of our recent ... [more ▼]
Immunogenetics, the analysis of genetic polymorphisms in specific recognition and immune regulation, is at the core of the study of host-parasite coevolution. We present here a synthesis of our recent works based on immune gene polymorphism to illustrate the power of this approach in improving our knowledge of rodent / hantavirus interactions. Two candidate genes (encoding Mx2 protein and β3 integrin) were first investigated at the inter-specific level. Sequence and/or expression polymorphism were compared among twenty murid species, some of them being reservoirs and some others being non reservoirs of hantaviruses. Our results showed that SNPs as well as patterns of selection acting on these genes differed between these rodent species and could mediate their susceptibility to hantaviruses. Four candidate genes (Mx2 gene and three genes from the Major Histocompatibility Complex) were next analyzed among populations of bank voles (Myodes glareolus), the reservoir of Puumala virus (PUUV). We revealed the existence of positive associations between Mhc alleles and PUUV infection in one hand and negative relationships between Tnf-α or Mx2 expression levels and PUUV prevalence in the other hand. These results suggested that tolerance to PUUV could have evolved in endemic areas. Such tolerance could be selected in response to the costs of immune responses that are activated against PUUV. We will conclude on the advantages to combine this immune gene candidate approach with more recent analyses based on genome scan, to deepen our investigation of this intra- and inter-specific variability of rodent tolerance / susceptibility to hantaviruses. [less ▲]Detailed reference viewed: 51 (2 ULg)
Immunogenicity of infliximab: how to handle the problem?
; ; Louis, Edouard et al
in Acta Gastro-Enterologica Belgica (2007), 70(2), 163-70
BACKGROUND: The introduction of infliximab has greatly advanced the therapeutic armamentarium of the inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis. Although the benefit/risk ... [more ▼]
BACKGROUND: The introduction of infliximab has greatly advanced the therapeutic armamentarium of the inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis. Although the benefit/risk ratio for infliximab is positive, of particular concern has been the problem of immunogenicity ascribed to the chimeric properties of the drug. Antibody formation is associated with allergic reactions and loss of response. AIMS AND METHODS: A literature search was undertaken on the magnitude of the problem of immunogenicity and on the clinical consequences. A survey was conducted about the clinical practice and management of acute and delayed allergic reactions to infliximab in different centres in Belgium. For this, a questionnaire was sent to all members of the Belgian IBD research group (n = 38 belonging to 29 centers). RESULTS AND CONCLUSION: Infusion reactions are important immunologic events induced by the presence of a substantial concentration of antibodies against infliximab (ATI) in the serum. Concomitant immunosuppressive treatment may optimize response to infliximab by preventing the formation of antibodies. Steroid administration prior to an infliximab infusion can further reduce the immunogenicity. Probably the most effective strategy to optimize treatment and avoid immunogenicity is maintenance therapy. If infliximab therapy can be discontinued is yet unclear but when treatment goals have been reached, we feel this should be attempted. In the case of relapse, infliximab should be restarted as maintenance long term. Practical guidelines on how to handle the problem of immunogenicity to infliximab are important for clinicians treating patients with IBD. [less ▲]Detailed reference viewed: 72 (0 ULg)
Immunogenicity of semisynthetic human insulin in man. Long-term comparison with porcine monocomponent insulin.
; ; et al
in Acta Diabetologica Latina (1986), 23(2), 101-6
The levels of circulating IgG-insulin antibodies were determined in two groups of diabetic patients before and at 3-month intervals after starting insulin treatment either with monocomponent porcine ... [more ▼]
The levels of circulating IgG-insulin antibodies were determined in two groups of diabetic patients before and at 3-month intervals after starting insulin treatment either with monocomponent porcine insulin (n = 17) or with human semisynthetic insulin (SH) (n = 16). Patients were followed during 15.1 +/- 1.0 and 19.9 +/- 1.1 months, respectively (m +/- SEM). In addition, the quality of metabolic control and residual B-cell function were evaluated in the group under treatment with SHI. The percentage of patients who remained antibody-free after 12-21 months of treatment was 67.75% in the human insulin-treated group and only 25-43% in the one receiving porcine insulin (p less than 0.01). Moreover, insulin antibody titers, when present, were usually lower in subjects treated with human insulin. In SHI-treated patients: metabolic control was excellent during the first months of treatment as evidenced by values of mean daily blood glucose (7.3 +/- 0.6 mmol/l), M-index according to Schlichtkrull (7.4 +/- 2.4) and Hb1c (6.8 +/- 0.6%); residual B-cell function, evaluated at 3-month intervals by a circadian profile of plasma C-peptide did not decrease throughout the study; and a significant deterioration of blood glucose control occurred after 18 months of treatment, which might have been due to a less intensive supervision of the patients by the physicians and/or less careful attention by the patients themselves. This observation confirms the need for a continuous education of the patients regardless of the type of insulin used. [less ▲]Detailed reference viewed: 39 (0 ULg)
Immunogenicity profile of a 3.75-Ug hemagglutinin pandemic rH5N1 split virion As03A-adjuvanted vaccine in elderly persons : a randomized trial
; ; et al
in Journal of infection diseases (2011), 203(8), 1054-1062Detailed reference viewed: 11 (2 ULg)
Immunoglobulin E enhances host resistance to venoms.
Conference (2015, September 07)Detailed reference viewed: 14 (1 ULg)
Immunoglobulines intraveineuses et maladie hémolytique allo-immune du nouveau-né.
Senterre, Thibault ; Viellevoye, Renaud ; Rigo, Jacques
in Archives de Pédiatrie (2010), 17(3), 299-300Detailed reference viewed: 70 (11 ULg)