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See detailMid-infrared prediction of cheese yield from milk and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Troch, Thibault ULg; Vanden Bossche, Sandrine et al

in Book of Abstracts of the 64th Annual Meeting of the European Federation of Animal Science (2013, August)

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See detailMid-infrared prediction of cheese yield from milk and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Troch, Thibault ULg; Vanden, Bossche et al

Conference (2013, August 29)

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See detailMid-infrared prediction of milk titratable acidity and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Vanlierde, Amélie ULg; Vanden Bossche, sandrine ULg et al

in Book of Abstracts of the 63rd Annual Meeting of the European Federation of Animal Science (2012, August)

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability ... [more ▼]

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability of this trait on a large scale, mid-infrared (MIR) chemometric methods were used to predict TA. A total of 507 milk samples collected in the Walloon Region of Belgium from individual cows were analyzed using a MIR spectrometer. TA was recorded as Dornic degree. An equation to predict TA from milk MIR spectrum was developed using partial least squared regression after a first derivative pre-treatment applied to the spectra to correct the baseline drift. During the calibration process, 45 outliers were detected and removed from the calibration set. The TA mean of the final calibration set was 16.62 (standard deviation (SD) = 1.80). The coefficient of determination (R²) was 0.82 for the calibration with a standard error (SE) of 0.76. A cross-validation (cv) was performed (R²cv = 0.81 with SEcv = 0.80). This equation was then applied on the spectral database generated during the Walloon routine milk recording. The variances components were estimated by REML using single-trait random regression animal test-day model. The dataset used included 33,717 records from 9,191 Holstein first-parity cows; the TA mean was 17.05 (SD = 1.35) and TA ranged from 12.83 to 20.87. Estimated daily heritabilities ranged from 0.43 at 5th day in milk to 0.59 at 215th day in milk indicating potential of selection. Further research will study phenotypic and genetic correlations between TA and milk production traits. [less ▲]

Detailed reference viewed: 33 (20 ULg)
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See detailMid-infrared prediction of milk titratable acidity and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Vanlierde, Amélie ULg; Vanden Bossche, sandrine ULg et al

Conference (2012, August 27)

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability ... [more ▼]

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability of this trait on a large scale, mid-infrared (MIR) chemometric methods were used to predict TA. A total of 507 milk samples collected in the Walloon Region of Belgium from individual cows were analyzed using a MIR spectrometer. TA was recorded as Dornic degree. An equation to predict TA from milk MIR spectrum was developed using partial least squared regression after a first derivative pre-treatment applied to the spectra to correct the baseline drift. During the calibration process, 45 outliers were detected and removed from the calibration set. The TA mean of the final calibration set was 16.62 (standard deviation (SD) = 1.80). The coefficient of determination (R²) was 0.82 for the calibration with a standard error (SE) of 0.76. A cross-validation (cv) was performed (R²cv = 0.81 with SEcv = 0.80). This equation was then applied on the spectral database generated during the Walloon routine milk recording. The variances components were estimated by REML using single-trait random regression animal test-day model. The dataset used included 33,717 records from 9,191 Holstein first-parity cows; the TA mean was 17.05 (SD = 1.35) and TA ranged from 12.83 to 20.87. Estimated daily heritabilities ranged from 0.43 at 5th day in milk to 0.59 at 215th day in milk indicating potential of selection. Further research will study phenotypic and genetic correlations between TA and milk production traits. [less ▲]

Detailed reference viewed: 67 (37 ULg)
See detailMid-infrared predictions of cheese yield from bovine milk
Vanlierde, Amélie ULg; Soyeurt, Hélène ULg; Anceau, Christine ULg et al

in Book of Abstracts of the 62nd Annual Meeting of the European Association for Animal Production (2011, August)

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be ... [more ▼]

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be interesting to predict CY during milk recording directly without the need to estimate milk components. Through the BlueSel project, 157 milk samples were collected in Wallonia from individual cows and analyzed using a mid-infrared (MIR) MilkoScanFT6000 spectrometer. Individual laboratory cheese yields (ILCY) were determined for each sample and expressed as g of dry coagulum/100 g of milk dry matter. An equation to predict ILCY from MIR was developed using partial least squared regression (Winisi III). A first derivative pre-treatment of spectra was used to correct the baseline drift. To improve the repeatability of the spectral data, a file which contained the spectra of samples analyzed on 5 spectrometers was used during the calibration. During calibration, 23 outliers were detected a nd removed from the calibration set. The ILCY mean of the final calibration set was 63.9% with a SD of 11.2%. The calibration (C) coefficient of determination (R²) was equal to 0.76 with a standard error (SE) of calibration of 5.5%. A full cross-validation (CV) was preformed to assess the robustness. R²cv was 0.72 with a SECV of 6.0%. The similarity between R²c and R²cv as well as between SEC and SECV permits to consider robustness of the developed equation as good. Even if it is planned to improve the equation with additional samples, this first equation will permit to study ILCY in the Walloon dairy cattle. [less ▲]

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See detailMid-infrared predictions of cheese yield from bovine milk
Vanlierde, Amélie ULg; Soyeurt, Hélène ULg; Anceau, Christine ULg et al

Conference (2011, August 31)

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be ... [more ▼]

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be interesting to predict CY during milk recording directly without the need to estimate milk components. Through the BlueSel project, 157 milk samples were collected in Wallonia from individual cows and analyzed using a mid-infrared (MIR) MilkoScanFT6000 spectrometer. Individual laboratory cheese yields (ILCY) were determined for each sample and expressed as g of dry coagulum/100 g of milk dry matter. An equation to predict ILCY from MIR was developed using partial least squared regression (Winisi III). A first derivative pre-treatment of spectra was used to correct the baseline drift. To improve the repeatability of the spectral data, a file which contained the spectra of samples analyzed on 5 spectrometers was used during the calibration. During calibration, 23 outliers were detected a nd removed from the calibration set. The ILCY mean of the final calibration set was 63.9% with a SD of 11.2%. The calibration (C) coefficient of determination (R²) was equal to 0.76 with a standard error (SE) of calibration of 5.5%. A full cross-validation (CV) was preformed to assess the robustness. R²cv was 0.72 with a SECV of 6.0%. The similarity between R²c and R²cv as well as between SEC and SECV permits to consider robustness of the developed equation as good. Even if it is planned to improve the equation with additional samples, this first equation will permit to study ILCY in the Walloon dairy cattle. [less ▲]

Detailed reference viewed: 58 (28 ULg)
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See detailMid-infrared predictions of fatty acids in bovine milk : final results of the RobustMilk project
Soyeurt, Hélène ULg; McParland, Sinead; Berry, Donagh et al

in Book of Abstracts of the 63rd Annual Meeting of the European Federation of Animal Science (2012, August)

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on ... [more ▼]

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on MIR spectra and FA from multiple countries, production systems, and breeds were used to develop equations to predict milk FA. The calibration set contained 1,776 spectrally different English, Irish, and Belgian milk samples collected for over 6 years. FA were quantified by gas chromatography (GC). Equations were built using partial least squares regression after a first derivative pretreatment applied to the spectral data. The robustness of the developed equations was assessed by cross-validation (CV) using 50 groups from the calibration set. The coefficient of determination (R²) obtained after CV ranged between 0.7101 for the total content of C18:2 and 0.9993 for the saturated FA group. The standard error of CV ranged between 0.0028 and 0.0998 g/dl of milk. Generally, the group or individual FA having the highest content in milk had the highest R²cv. The results obtained in this study confirmed the usefulness of MIR spectra to robustly quantify the FA content of milk permitting the use of these equations by milk laboratories in UK, Belgium or Ireland. Therefore, these equations could be used to develop selection or management tools for dairy farmers in order to improve the nutritional and environmental quality of milk based on the knowledge of the FA composition of their milk. [less ▲]

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See detailMid-infrared predictions of fatty acids in bovine milk : final results of the RobustMilk project
Soyeurt, Hélène ULg; McParland, Sinead; Berry, Donagh et al

Poster (2012, August 28)

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on ... [more ▼]

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on MIR spectra and FA from multiple countries, production systems, and breeds were used to develop equations to predict milk FA. The calibration set contained 1,776 spectrally different English, Irish, and Belgian milk samples collected for over 6 years. FA were quantified by gas chromatography (GC). Equations were built using partial least squares regression after a first derivative pretreatment applied to the spectral data. The robustness of the developed equations was assessed by cross-validation (CV) using 50 groups from the calibration set. The coefficient of determination (R²) obtained after CV ranged between 0.7101 for the total content of C18:2 and 0.9993 for the saturated FA group. The standard error of CV ranged between 0.0028 and 0.0998 g/dl of milk. Generally, the group or individual FA having the highest content in milk had the highest R²cv. The results obtained in this study confirmed the usefulness of MIR spectra to robustly quantify the FA content of milk permitting the use of these equations by milk laboratories in UK, Belgium or Ireland. Therefore, these equations could be used to develop selection or management tools for dairy farmers in order to improve the nutritional and environmental quality of milk based on the knowledge of the FA composition of their milk. [less ▲]

Detailed reference viewed: 51 (11 ULg)
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See detailMid-infrared predictions of lactoferrin content in bovine milk
Soyeurt, Hélène ULg; Bastin, Catherine ULg; Colinet, Frédéric ULg et al

in Journal of Dairy Science (2011), 94(E-suppl.1), 714

Lactoferrin (LF) is a glycoprotein present in milk and active in the immune system of cows and humans. Therefore, an inexpensive and rapid analysis to quantify this protein is desirable. A previous study ... [more ▼]

Lactoferrin (LF) is a glycoprotein present in milk and active in the immune system of cows and humans. Therefore, an inexpensive and rapid analysis to quantify this protein is desirable. A previous study reported the potential to quantify LF from the mid-infrared (MIR) spectrometry from 69 milk samples. Through the European RobustMilk project (www.robustmilk.eu), 3,606 milk samples were collected in Belgium, Ireland, and Scotland from individual cows and analyzed using a MIR MilkoScanFT6000 spectrometer. Milk LF content was quantified using ELISA in duplicate. Average ELISA data with a CV lower than 5% were used. After the detection of spectral and ELISA outliers, the calibration set contained 2,499 samples. An equation to predict LF content from MIR was developed using partial least squared regression. A first derivative pre-treatment of spectra was used to correct the baseline drift. To improve the repeatability of the spectral data, a file which contained the spectra of samples analyzed on 5 spectrometers was used during the calibration. The lactoferrin mean was 159.28 mg/l of milk with a SD of 97.21 mg/l of milk. The calibration (C) coefficient of determination (R2) was equal to 0.73 with a standard error (SE) of calibration of 50.54 mg/l of milk. A cross-validation (CV) was used to assess the robustness of the equation. R2 CV was 0.72 with a SE-CV of 51.16 mg/l of milk. An external validation (V) was conducted on 150 milk samples collected in Belgium. The SE of prediction (SEP) was 59.17 mg/L of milk. The similarity between R2 C and R2CV as well as between SE-C and SE-CV and between SE-CV and SEP confirms the equations developed are robust. The correlation between predicted and measured LF values was 0.71. This lower value compared with the one obtained from the calibration set (0.85) could be explained by the low ELISA reproducibility (16.24% ± 25.51%). If the developed equation is used to clean the validation data set, a total of 16 samples can be deleted. The validation coefficient for these 134 samples increased to 0.82. From these results, the developed equation could be used for screening the dairy cow population for breeding purposes. [less ▲]

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See detailMid-Infrared Spectroscopy Coupled with Chemometrics: A Tool for the Analysis of Intact Food Systems and the Exploration of Their Molecular Structure-Quality Relationships - A Review
Karoui, Ramdhane; Downey, Gerard; Blecker, Christophe ULg

in Chemical Reviews (2010), 110(10), 6144-6168

Public interest in food quality and methods of production has increased significantly in recent decades, due in part to changes in eating habits, consumer behavior, and the increased industrialization and ... [more ▼]

Public interest in food quality and methods of production has increased significantly in recent decades, due in part to changes in eating habits, consumer behavior, and the increased industrialization and globalization of food supply chains.1 Demand for high levels of quality and safety in food production obviously requires high standards in quality assurance and process control; satisfying this demand in turn requires appropriate analytical tools for food analysis both during and after production. Desirable features of such tools include speed, ease-of-use, minimal or no sample preparation, and the avoidance of sample destruction. These features are characteristic of a range of spectroscopic methods including the mid-infrared (MIR). While it is true that near-infrared (NIR) spectroscopy has achieved greater uptake by the food industry,2 reported applications of MIR in this sector have increased over the past decade or more. Foods represent significant analytical challenges. They are highly complex, variable and can be found in a number of different physical states: these include solids, dilute solutions, emulsions, foams, highly visco-elastic forms, and glassy [less ▲]

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See detailMid-Miocene paleoproductivity in the Atlantic Ocean and implications for the global carbon cycle
Diester-Haass, Liselotte; Billups, Katharina; Groecke, Darren R et al

in Paleoceanography (2009), 24

A prominent, middle Miocene (17.5-13.5 Ma) carbon isotope excursion ubiquitously recorded in carbonate sediments has been attributed to enhanced marine productivity and sequestration of C-13 depleted ... [more ▼]

A prominent, middle Miocene (17.5-13.5 Ma) carbon isotope excursion ubiquitously recorded in carbonate sediments has been attributed to enhanced marine productivity and sequestration of C-13 depleted organic carbon in marine sediments or enhanced carbon burial in peat/lignite deposits on land. Here we test the hypothesis that the marine delta C-13 record reflects a change in productivity with proxy records from three Atlantic Ocean sites (Deep Sea Drilling Program Site 608 and Ocean Drilling Program Sites 925 and 1265). Our multiproxy approach is based on benthic foraminiferal accumulation rates, elemental ratios (Ba/Al and P/Al), the delta C-13 of bulk sedimentary organic matter, and dissolution indices. We compare these proxies to benthic foraminiferal delta C-13 values measured on the same samples. Our results indicate that marine paleoproductivity in the Atlantic Ocean is not related to the benthic foraminiferal delta C-13 excursion. A numerical box model confirms that marine productivity cannot account for the delta C-13 maximum. The model shows that sequestration of 1.5 x 10(18) mol C in the terrestrial realm over a period of 3 Ma leads to a 0.9 parts per thousand delta C-13 increase in the deep ocean, which is near the observed records. Therefore, an increase in continental organic carbon sequestration is the most plausible way to enrich the ocean's carbon pool with C-13, which is consistent with coeval lignite deposits worldwide. The delta C-13 values of bulk sedimentary organic matter parallel the delta C-13 of dissolved inorganic carbon as reflected by benthic foraminiferal delta C-13 values suggesting no significant change in atmospheric pCO(2) levels over the investigated period. [less ▲]

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See detailA mid-size city and IYA09: a case study
Nazé, Yaël ULg

Poster (2010)

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See detailMid-term economical consequences of roadside tree topping
Campanella, Bruno ULg; Toussaint, André ULg; Paul, Roger ULg

in Urban Forestry and Urban Greening (2009), 8

Detailed reference viewed: 38 (13 ULg)
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See detailMid-term follow-up after multiple system organ failure following cardiac surgery in children.
Heying, R.; SEGHAYE, Marie-Christine ULg; Grabitz, R. G. et al

in Acta Paediatrica (1999), 88(11), 1238-43

Multiple system organ failure after cardiac surgery in children is a severe complication with unknown mid- and long-term sequelae. We therefore evaluated 11 children (aged 20-126 mo, median: 67 mo) having ... [more ▼]

Multiple system organ failure after cardiac surgery in children is a severe complication with unknown mid- and long-term sequelae. We therefore evaluated 11 children (aged 20-126 mo, median: 67 mo) having survived multiple system organ failure after cardiac operations for congenital cardiac defects in a cross-sectional follow-up study 12-76 mo (median: 32 mo) after surgery. Clinical and laboratory examinations included cardiac, pulmonary, renal, hepatic, neurological and psychological function tests. All patients had adequate cardiac function. Lung mechanics were abnormal in three children and glomerular renal function was abnormal in two patients. Slight elevation of gamma-glutamyl transpeptidase and coagulation factor deficiency was present in six and seven patients, respectively (five of whom had undergone the Fontan operation). Severe neurological sequelae such as diplegia (n = 1) and mental retardation (n = 1) were observed in two patients. In addition, five children presented delayed motor, graphomotor and/or speech development. Two children were found to have abnormal intelligence. We conclude that with the exception of neurological impairment, mid-term sequelae of multiple system organ failure after cardiac surgery in children are mild. However, longer follow-up using an appropriate control group is mandatory. [less ▲]

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See detailMid-term results of a bipolar radial head prosthesis: Radiographic evidence of loosening at the cement-bone interface
Popovic, Nebojsa; Georis, Pierre ULg; lemaire, Roger et al

in Journal of Bone & Joint Surgery. American Volume (2007), 89-A(11), 2469-2476

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Peer Reviewed
See detailMiddle and Upper Devonian Miospore Zonation of Eastern Europe.
Avkhimovitch, V.I.; Tchibrikova, E.V.; Obukhovskaya, T.G. et al

in Bull. Centres Rech. Explor.-Prod. Elf Aquitaine, (1993), 17

Detailed reference viewed: 8 (0 ULg)