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See detailhybridization behaviour between two common European cyprinid fish species - silver bream Blicca bjoerkna and common bream Abramis brama - in a controlled environment
Nzau Matondo, Billy; Ovidio, Michaël ULg; Philippart, Jean-Claude ULg et al

in Animal Biology (2009), 59

The egg release–mating comparison, heterospecific matings and mating success under two hybridization conditions – (i) mixing one sex per species and (ii) mixing both sexes from each species – were ... [more ▼]

The egg release–mating comparison, heterospecific matings and mating success under two hybridization conditions – (i) mixing one sex per species and (ii) mixing both sexes from each species – were investigated to determine whether silver bream Blicca bjoerkna and common bream Abramis brama can hybridize in nature. The results revealed that non-matings in hybridization experiments of silver bream females × common bream males can be explained by territorial and aggressiveness activities observed in common bream. In common bream females × silver bream males, heterospecific matings were observed but their numbers were signifi cantly lower than the spawning numbers, and in this experiment, a female mated with one to four heterospecifi c males. In mixing both sexes from both species, similar spawning – mating numbers were observed but heterospecific matings accounted for only 27% of the total matings, with 24% accounting for heterospecifi c matings between common bream females and silver bream males, directly or by opportunism. Mating success was characterized by the occurrence of fertilized eggs after matings. Natural hybridization occurred preferentially between common bream females and silver bream males. [less ▲]

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See detailHybridization capture using short PCR products enriches small genomes by capturing flanking sequences (CapFlank)
Tsangaras, K.; Wales, N.; Sicheritz-Pontén, T. et al

in PLoS ONE (2014), 9(10),

Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates ... [more ▼]

Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments, a non-negligible fraction of the resulting sequence reads are not homologous to the bait. We demonstrate that during capture, the bait-hybridized library molecules add additional flanking library sequences iteratively, such that baits limited to targeting relatively short regions (e.g. few hundred nucleotides) can result in enrichment across entire mitochondrial and bacterial genomes. Our findings suggest that some of the off-target sequences derived in capture experiments are non-randomly enriched, and that CapFlank will facilitate targeted enrichment of large contiguous sequences with minimal prior target sequence information. [less ▲]

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See detailHybridization effects in U2T2X compounds: Magnetic structures of U2Rh2Sn and U2Ni2In
Nakotte, Heinz; Purwanto, A.; Robinson, R. A. et al

in Physical Review. B : Condensed Matter (1996), 53(6), 3263-3271

The tetragonal intermetallic compounds U2Ni2In and U2Rh2Sn have been studied by means of specific-heat, electrical-resistivity, and neutron-diffraction techniques. At low temperatures, both compounds ... [more ▼]

The tetragonal intermetallic compounds U2Ni2In and U2Rh2Sn have been studied by means of specific-heat, electrical-resistivity, and neutron-diffraction techniques. At low temperatures, both compounds order antiferromagnetically in a magnetic unit cell doubled along the c axis, and we find 5f moments of 0.60 and 0.38 Bohr magneton/U atom for U2Ni2In and U2Rh2Sn, respectively. For U2Ni2In, our refinement also indicates a possible Ni moment of 0.37 Bohr magneton perpendicular to the U moments. We discuss the development of the 5f moments together with findings on other isostructural U2T2X (T=transition metal, X=In, Sn) compounds, and confirm that the trends expected due to 5f-ligand hybridization also hold for this family of uranium compounds. Our analysis indicates different arrangements of the 5f moments in the two compounds (noncollinear arrangement within the basal plane for U2Ni2In and collinear arrangement along the c axis for U2Rh2Sn), although both compounds have nearest-neighbor U-U distances along the c axis. This would mean that the magnetocrystalline anisotropy in U2T2X compounds is not determined by nearest-neighbor U-U links alone [less ▲]

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See detailHybridization of 2,659 Clostridium Perfringens Isolates with Gene Probes for Seven Toxins (Alpha, Beta, Epsilon, Iota, Theta, Mu, and Enterotoxin) and for Sialidase
Daube, Georges ULg; Simon, Patricia ULg; Limbourg, Bernard et al

in American Journal of Veterinary Research (1996), 57(4), 496-501

OBJECTIVE--To genetically characterize Clostridium perfringens isolates for association of pathologic type with various diseases. DESIGN--Prospective study. SAMPLE POPULATION--2,659 C perfringens isolates ... [more ▼]

OBJECTIVE--To genetically characterize Clostridium perfringens isolates for association of pathologic type with various diseases. DESIGN--Prospective study. SAMPLE POPULATION--2,659 C perfringens isolates from various nonhuman animals species, human beings, and foods. PROCEDURE--Colony hybridization with DNA probes for 7 toxin (alpha, beta, epsilon, iota (subunits a and b), theta, mu, and enterotoxin) genes and 1 sialidase gene were performed to group the isolates by pathologic type. RESULTS--Enterotoxin-negative type-A isolates were the most common (2,575/2,659), were isolated from all sources, and were separated into 5 pathologic types. In cattle and horses with enterotoxemia, essentially only these pathologic types were identified. The enterotoxin-negative isolates of types C or D each had a single pathologic type. Type-C isolates were isolated only from swine with necrotic enteritis and type-D isolates from small ruminants with enterotoxemia, except that 1 type-D isolate was also found from a healthy fish. Type-B or -E isolates were not found. Among the 47 enterotoxin-positive isolates, 5 isolates from sheep or deer were type D and the other 42 were type A. These 42 isolates were grouped into 3 pathologic types: 1 type was isolated from samples of almost all origins, but the other 2 types were found in only 5 fish, 4 human beings, and 1 dog. CONCLUSIONS AND CLINICAL RELEVANCE--Genetic characterization of these isolates allowed identification of 11 different pathologic types. This approach may be useful in molecular diagnosis and prophylaxis of clostridial disease. [less ▲]

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See detailHybridization of bovine Escherichia coli isolates with genes probes for four enterotoxins (StaP, STaH, STb, LT) and one adhesive factor (K99)
Mainil, Jacques ULg; Moseley, S. L.; Schneider, R. A. et al

in American Journal of Veterinary Research (1986), 47

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See detailHybridization of short complementary PNAs to G-quadruplex forming oligonucleotides: An electrospray mass spectrometry study.
Amato, Jussara; Oliviero, Giorgia; De Pauw, Edwin ULg et al

in Biopolymers (2009), 91(4), 244-255

We investigated the interaction of the short PNA strand [acccca]-PNA with oligodeoxynucleotides containing one, two, or four tracts of TGGGGT units. Electrospray ionization mass spectrometry allowed ... [more ▼]

We investigated the interaction of the short PNA strand [acccca]-PNA with oligodeoxynucleotides containing one, two, or four tracts of TGGGGT units. Electrospray ionization mass spectrometry allowed exploring the wide variety of complex stoichiometries that were found to coexist in solution. In water, the PNA strand forms short heteroduplexes with the complementary DNA sequences, but higher-order structures are also found, with PNA(2n)*DNA(n) triplex units, culminating in precipitation at very low ionic strength. In the presence of ammonium acetate, there is a competition between PNA*DNA heteroduplex formation and DNA G-quadruplex formation. Heteroduplex formation is favored when the PNA + DNA mixture in ammonium acetate is heated and cooled at room temperature, but not if the PNA is added at room temperature to the pre-formed G-quadruplex. We also found that the short [acccca]-PNA strand binds to G-quadruplexes. (c) 2008 Wiley Periodicals, Inc. Biopolymers, 2008. [less ▲]

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See detailHybridization on bovine enterotoxigenic Escherichia coli with two heat-stable enterotoxin gene probes
Mainil, Jacques ULg; Bex, F.; Couturier, M. et al

in American Journal of Veterinary Research (1985), 46

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See detailHybridization success of three common European cyprinid species, Rutilus rutilus, Blicca bjoerkna and Abramis brama and larval resistance to stress tests
Nzau Matondo, Billy; Ovidio, Michaël ULg; Poncin, Pascal ULg et al

in Fisheries Science (2007), 73(5), 1137-1146

Hybridization success at early developmental stages and larval resistance to osmotic, thermal and fasting tests in roach Rutilus rutilus, silver bream Blicca bjoerkna, common bream Abramis brama and their ... [more ▼]

Hybridization success at early developmental stages and larval resistance to osmotic, thermal and fasting tests in roach Rutilus rutilus, silver bream Blicca bjoerkna, common bream Abramis brama and their F1 hybrids were investigated. Results revealed that hybrid survival rates were similar to parents. At the eyed embryo stage, however, a maternal effect was observed as a general trend during hatchling and larval stages. After these stages, hybrids displayed a higher survival rate than their parents. Under stress tests, no survival was observed after 40 min for osmotic and thermal shocks and after 24 days for the prolonged fasting test in these species and their F1 hybrids. The median survivals of hybrids were intermediate between the two parents. For total mortality, hybrids were also affected by a maternal effect but to the advantage of the hybrids. [less ▲]

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See detailHydoelastic analysis using the finite element method
Piret, G.; Debongnie, Jean-François ULg; Géradin, Michel ULg

Report (1992)

Part 1 : The finite element formulation for fluid-structure problems - To the best of our knowledge, the first purely lagrangian formulation was proposed by Debongnie. At this time, even though natural ... [more ▼]

Part 1 : The finite element formulation for fluid-structure problems - To the best of our knowledge, the first purely lagrangian formulation was proposed by Debongnie. At this time, even though natural, this idea as well as the notion of displacement potential led to some surprises. Thes rationalizing efforts led to the final formulation of the corrected Tong's formulation in 1978 and to the corresponding formulation for the compressible case in 1984. Similar results were obtained by Morand in 1987. Here, contrarily to our preceeding papers, we present the formulation from a local point of view instead of a variational one. PART 2 : On the representation of rigid body modes with the SAMCEF formulation for hydroelastic problems - The question to see wether the SAMCEF formulation of hydroelastic problems respects the rigid body modes is often raised. It appear very often that some of the rotational rigid body modes are lost for the fre-free problem. The present report tries to give a better understanding of this problem. PART 3 - Dynamic analysis of fluid-structure problems : results. [less ▲]

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See detailHydratation and electrolyte balance in horses during an endurance season
Robert, Céline; Goachet, Anne-Gaëlle; Fraipont, Audrey ULg et al

in Equine Veterinary Journal. Supplement (2010), 42(38), 98-104

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See detailHydrates de gaz dans les noyaux cométaires et les grains interstellaires
Delsemme, A.-H.; Swings, Polydore ULg

in Annales d'astrophysique (1952), 15(1), 1-6

Solid hydrates of CH4, CO2, ... may be present in cometary nuclei and interstellar grains. The vapor pressures — hence the rates of sublimation — of the various hydrates are of the same order of magnitude ... [more ▼]

Solid hydrates of CH4, CO2, ... may be present in cometary nuclei and interstellar grains. The vapor pressures — hence the rates of sublimation — of the various hydrates are of the same order of magnitude while the vapor pressures of solid CH4, H2O, ... differ by very large factors. The relative abundances of the radicals which are observed in comets would be explained more satisfactorily by assuming the presence of solid hydrates. [less ▲]

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See detailHydration kinetics of compacted low-density bentonite
Dieudonné, Anne-Catherine ULg; Romero, Enrique; Charlier, Robert ULg

in Khalili, Nasser; Russel, Adrian; Khoshghalb (Eds.) Unsaturated Soils: Research and Applications (2014)

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See detailHydraulic and wood traits of two congeneric tropical tree species in their core habitat
Gorel, Anaïs ULg; Steppe, kathy; Beeckman, Hans et al

Conference (2017, February 06)

Background: Strong niche partitioning across rainfall gradients has been identified for several tropical tree genera. The link between hydraulic and wood anatomical traits, associated with drought ... [more ▼]

Background: Strong niche partitioning across rainfall gradients has been identified for several tropical tree genera. The link between hydraulic and wood anatomical traits, associated with drought tolerance, however remains to be explored, in order to identify the mechanisms shaping the range limits of tropical tree species. Aim: In this study, we aimed to identify the differences in hydraulic and wood traits between two congeneric tree species with contrasting distributions in moist and wet tropical forests. Location: Central African moist and wet forests Methods: In the core habitat of Erythrophleum ivorense (wet forest) and of E. suaveolens (moist), we collected branches to construct vulnerability curves and measure hydraulic capacitance, and both stem and branch wood samples to link the hydraulic traits to wood anatomy. Major results: E. suaveolens, which is characteristic of drier forests, is clearly more resistant to cavitation than E. ivorense, and also possess a greater hydraulic capacitance (i.e. the capacity that species have to mitigate periods of water storage by using internally stored water). In agreement with this great drought tolerance for E. suaveolens, wood anatomy revealed a high number of small vessels associated with small intervessel pits, features minimizing cavitation risk but also reducing water transport. Main conclusions: Drought tolerance, as indicated by both hydraulic and wood traits, strongly differed between the closely related species and explained their contrasting distribution, and affinity for moist (E. ivorense) and wet (E. suaveolens) forests. However, phenotypic plasticity in hydraulic and wood traits remained to be addressed to examine the extent of water use differences between the two species. [less ▲]

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See detailHydraulic behavior of Piano Key Weirs: experimental approach
Machiels, Olivier ULg; Erpicum, Sébastien ULg; Archambeau, Pierre ULg et al

in Janssen, Robert; Chanson, Hubert (Eds.) 3rd Int. Junior Researcher and Engineer Worjshop on Hydraulic Structures (2010, May)

The Piano Key Weir (PKW) is a particular geometry of weir similar to a labyrinth shape that features overhanging cycle ends to reduce the base length. Together with its high discharge capacity for low ... [more ▼]

The Piano Key Weir (PKW) is a particular geometry of weir similar to a labyrinth shape that features overhanging cycle ends to reduce the base length. Together with its high discharge capacity for low heads, this geometric feature makes the PKW an interesting solution for dam rehabilitation, permitting the placement of the weir directly on dam crest. However, PKW stays a new type of weir, firstly designed in 2001 and built for the first time in 2006 by Electricité de France (EDF). Even if the first experimental studies confirmed its discharge capacities, lacks persist in the understanding of the flow behaviour upstream, along and downstream of this complex structure. This paper presents the mean results and observations obtained from experiments on a large scale model of PKW. The hydraulics of the structure is clarified based on measurements of water depths, pressures, velocities and discharges on each part of the weir. These observations explain the effect of geometric improvements proposed in former studies. [less ▲]

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See detailHydraulic behaviour of greened porous pavements: a physical study
Straet, Frederic; Beckers, Eléonore ULg; Degre, Aurore ULg

(2008)

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See detailHydraulic modelling in civil and environmental engineering
Dewals, Benjamin ULg; Erpicum, Sébastien ULg; Archambeau, Pierre ULg et al

Scientific conference (2013, May 08)

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See detailHydraulic modelling along river Ourthe
Dewals, Benjamin ULg; Detrembleur, Sylvain ULg; Archambeau, Pierre ULg et al

Conference (2007, June 01)

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See detailHydraulic modelling along river Ourthe considering impacts of climate change
Dewals, Benjamin ULg

Conference (2007, June 01)

Detailed reference viewed: 4 (1 ULg)