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See detailIdentification of methylenecyclopropyl acetic acid in serum of European horses with atypical myopathy
Votion, Dominique ULg; van Galen, G; Sweetan et al

in Equine Veterinary Journal (2013)

Detailed reference viewed: 211 (39 ULg)
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See detailIdentification of micrometastasis of breast carcinomas by presence of basement membrane collagen.
Liotta, L. A.; Foidart, Jean-Michel ULg; Gehron Robey, P. et al

in Lancet (1979), 2(8134), 146-7

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See detailIdentification of microRNA-based signatures for response and survival for non-small cell lung cancer treated with cisplatin-vinorelbine A ELCWP prospective study
Berghmans, T; Ameye, L; Willems, Luc ULg et al

in Lung Cancer (2013), 82

Clinical variables, like stage and performance status (PS), have predictive and prognostic values in advanced non-small cell lung cancer (NSCLC) patients treated with chemotherapy, not allowing adequate ... [more ▼]

Clinical variables, like stage and performance status (PS), have predictive and prognostic values in advanced non-small cell lung cancer (NSCLC) patients treated with chemotherapy, not allowing adequate individual prediction. MicroRNA (miRNA) are non-coding RNAs regulating gene expression. In a prospective study, we assessed the predictive value for response and survival of tumour miRNA in NSCLC patients treated by 1st line cisplatin and vinorelbine. miRNA expression was analysed on a biopsy obtained during the diagnostic bronchoscopy, using TaqMan Low Density Arrays. The signature for response was derived using logistic regression with stepwise variable selection. The associations between overall survival and miRNA expression levels were estimated by using the Kaplan–Meier method, log-rank test, and Cox proportional hazard regression models to estimate the hazard ratios. In total, 38 patients with adequate tumour biopsies, treated with cisplatin-vinorelbine were included: male (n = 27), 80–100 Karnofsky PS (n = 27), adenocarcinoma (n = 20), stage IV (n = 30). One patient was considered not assessable for response but remained included in the survival analyses. Out of the 37 patients assessable for response, 16 partial responses (43%) were observed. A two miRNA signature (miR-149 and miR-375) was found predictive for response and was also associated to progression-free survival (p = 0.05). Using a linear combination of the miR CT values with Cox’s regression coefficients as weights, we constructed a prognostic score for overall survival including four miRNA (miR-200c, miR-424, miR-29c and miR-124). The signature distinguished patients with good (n = 18) and poor (n = 20) prognosis with respective median survival times of 47.3 months (95% CI 29.8–52.4) and 15.5 months (95% CI 9.1–22.8) (p < 0.001; hazard ratio 21.1, 95% CI 4.7–94.9). Conclusions: miRNA signature allows predicting response and is of prognostic value for survival in patients with NSCLC treated with first line cisplatin and vinorelbine. [less ▲]

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See detailIdentification of Mistuning and Model Updating of an Academic Blisk Based on Geometry and Vibration Measurements
Nyssen, Florence ULg; Golinval, Jean-Claude ULg

in Mechanical Systems & Signal Processing (2015)

In this work, an experimental modal analysis is performed on an academic bladed disk using a base excitation to identify the mistuning of each blade. Optical measurement is used to obtain the exact ... [more ▼]

In this work, an experimental modal analysis is performed on an academic bladed disk using a base excitation to identify the mistuning of each blade. Optical measurement is used to obtain the exact geometry of the structure and to be able to associate geometrical mistuning to each blade. Differences are observed between the experimentally identified mistuning and the geometrical mistuning. Since the bladed disk is a one-piece structure, there are no welded connections between the blades and the disk and the material properties can be assumed to be uniform. It can be shown that these differences come from nonuniform clamping conditions, and that this mistuning is of the same order of magnitude than the variations in the geometry of the structure. It follows that the precise characterisation of mistuning for industrial structures is in practice illusory because of the numerous factors introducing mistuning, such as the clamping conditions, aerodynamic damping, wear in service, etc. [less ▲]

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See detailIdentification Of Mitochondrial Plasmid Like Dnas In Picea Abies (L.) Karst.
Chandelier, A.; Du Jardin, Patrick ULg; Avril, C. et al

in Plant Cell Reports (1999), 18(10),

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See detailIdentification of modulators for SUCNR1 by screening of a SOSA library with a bioluminescent cAMP assay
Gilissen, Julie; Dupuis, Nadine ULg; Derj, Anouar et al

Conference (2014, November 21)

Background Succinic acid (SA), a metabolic component that takes part in the citric acid cycle, has been described as the cognate agonist for the orphan receptor SUCNR1 (GPR91). This receptor belongs to ... [more ▼]

Background Succinic acid (SA), a metabolic component that takes part in the citric acid cycle, has been described as the cognate agonist for the orphan receptor SUCNR1 (GPR91). This receptor belongs to the G Protein-Coupled Receptor family (GPCR), that play an essential role in regulating many physiological functions and represent 30% of targets for currently marketed drugs. Several studies on KO models suggested different roles for SUCNR1 through its inactivation. Nevertheless, the characterization of the pharmacology and physiology of SUCNR1 is limited by the lack of small molecules used as pharmacological tools. Methods In order to identify ligands modulating SUCNR1 Gαi activity, we adapted a specific cAMP assay based on a modified luciferase fused with cAMP binding domain (Glosensor® promega corporation). Upon cAMP binding, conformational changes induce luminescent signal. This sensitive assay was carried out in a real time measurements fashion. It was compatible with the screening of chemical libraries. We utilized a SOSA library based on the principle that active compounds might have an activity on new targets at high concentration. Results We performed a primary agonist screening on 1280 compounds of a SOSA library at two different concentrations (100 and 10µM) with a cAMP assay (Z’=0,4-0,6). We selected 114 out of them that were characterized at least by an increase (or decrease) of 20% of the luminescent signal from HEK293.SUCNR1 cells. These compounds were subjected to a secondary screening performed in triplicates. Results analysis provided 16 putative modulators of SUCNR1 Gαi activity. We followed a similar strategy in another screening to find candidates with an antagonist profile. In parallel, we docked a part of the ZINC database ‘‘lead-like’’ molecules against SUCNR1 protein built by homology modelling from the β2-adrenergic receptor. This virtual screening has sorted 30 compounds with substantial theoretical affinity. They are currently undergoing functional evaluation in the cAMP assay. Conclusions We set up a real-time luminometric cAMP assay for SUCNR1. We selected a dozen of putative modulators of SUCNR1 that were selected for thorough evaluation. Furthermore, we obtained 30 potentially active compounds from a virtual screening that are assayed for functional activity at the receptor. The pharmacology of hits is currently characterized with different models and assays. [less ▲]

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See detailIdentification of molecular components of the host-microbiota-connectome by using "Omics Approaches"
Mariman, Rob ULg; Coppieters, Wouter ULg; Elansary, Mahmoud ULg et al

Poster (2014, April 24)

The host immune system plays an critical role in maintaining homeostasis with resident microbial communities, therefore ensuring that the complex symbiotic relationship is maintained. At the same time ... [more ▼]

The host immune system plays an critical role in maintaining homeostasis with resident microbial communities, therefore ensuring that the complex symbiotic relationship is maintained. At the same time, resident microbiota contribute to host nutrition and energy balance and to the development or maintenance of a robust immune system. Dysbiosis of the microbiota is associated with various immunological disorders, including inflammatory bowel diseases (IBD). Both genetic and environmental factors are implicated in this disturbance; however, the relative contributions of these two factors, and the mechanism by which they interact remain unclear. Recently, we started a project that aims to identify molecular components of the hostmicrobiota-connectome by taking advantage of common variation in – on the one hand – the genome, transcriptome and metabolome of the host, and – on the other hand – the composition of its gut microbiota. We will take advantage of the already established CEDAR cohort that provides integrated genetic (SNP genotypes) and transcriptome data (circulating immune cells subset, as well as samples from various anatomical locations in the intestine). We will further enrich the dataset in this cohort with metabolome (plasma), and gut microbiota data (16srRNA sampled at the ileum, colon, and rectum). The CEDAR cohort is composed of healthy individuals and is therefore more suitable to study effect of common risk variants than (IBD) patients, since analysis of samples from patients suffering from active inflammation may only give insight in ongoing patho-physiological processes, that are likely to mask the primum movens events. Next, we will study the overlap between the identified components of the HMC network identified and the ~160 GWAS-identified risk loci for IBD. We anticipate to reveal novel connections between the microbiota and IBD by this integrative “omics” approach, thereby shedding new light on the pathogenesis of IBD. Latest results will be presented with respect to the microbiota composition of from different anatomical locations in the intestine using the V2 and V5-6 regions of the bacterial 16S rRNA. [less ▲]

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See detailIdentification of multi-degree of freedom non-linear systems using an extended modal space model
Platten, Michael F; Wright, Jan Robert; Dimitriadis, Grigorios ULg et al

in Mechanical Systems & Signal Processing (2009), 23(1), 8-29

The identification of non-linear dynamic systems is an increasingly important area of research, with potential application in many industries. Current non-linear identification methodologies are, in ... [more ▼]

The identification of non-linear dynamic systems is an increasingly important area of research, with potential application in many industries. Current non-linear identification methodologies are, in general, mostly suited to small systems with few degrees of freedom and few non-linearities. In order to develop a practical identification approach for real engineering structures, the capability of such methods must be significantly extended. In this paper, it is shown that such an extension can be achieved using multi-exciter techniques in order to excited specific modes or degrees of freedom of the system under investigation. A novel identification method for large non-linear systems is presented, based on the use of a multi-exciter arrangement using appropriated excitation applied in bursts. This proposed Non-linear Resonant Decay Method is applied to a simulated system with 5 degrees of freedom and an experimental clamped panel structure. The technique is essentially a derivative of the Restoring Force Surface method and involves a non-linear curve fit performed in modal space. The effectiveness of the resulting reduced order model in representing the non-linear characteristics of the system is demonstrated. The potential of the approach for the identification of large continuous non-linear systems is also discussed. [less ▲]

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See detailIdentification of multiple pregnancy-associated glycoproteins (PAGs) purified from the European bison (Eb; Bison bonasus L.) placentas.
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Animal Reproduction Science (2008), 112

This paper describes the first identified chorionic PAGs in the European bison (Eb), named EbPAGs, predominantly expressed during early and mid-pregnancy (45-120 day post-coitum; dpc). Many EbPAGs were ... [more ▼]

This paper describes the first identified chorionic PAGs in the European bison (Eb), named EbPAGs, predominantly expressed during early and mid-pregnancy (45-120 day post-coitum; dpc). Many EbPAGs were extracted from various cotyledonary tissues, precipitated, chromatographed (DEAE and VVA: Vicia villosa agglutinin), electrophoresed (1D- and 2D-PAGE), analysed by heterologous (cross-species) Western blotting and then micro-sequenced by Edman degradation. Finally, twelve selected VVA-purified isoforms (Ip 3.7-7.4) were entirely characterised. Nine identified NH(2)-terminal micro-sequences were found to be PAGs. On 45dpc, three identified forms were named: EbPAG(67AkDa) (RGSNLTHPLRNIGDLFYVGN), EbPAG(55BkDa) (RGSNLTHPL) and EbPAG(50CkDa) (SQISLRGSNLTI). On 60dpc, the next three forms were named: EbPAG(71DkDa) (RGSNLTIHPLRNIIDLFYVG), EbPAG(55EkDa) (RGSNLTHPLRNI) and EbPAG(50FkDa) (SQISLRGS). On 120dpc, three other forms were named: EbPAG(71GkDa) (RGSNLTHPLRNIRDLFYVG), EbPAG(60HkDa) (RGSNLTTHPLRNIKDLVVYM) and EbPAG(50IkDa) (SGSNLTTV). These EbPAG ((A-I)) sequences are unique, as they are not identical to any other PAGs purified previously in related species of the Bovidae family. However, the EbPAGs (A-I forms) have some sequence resemblance to internal sequences of various full-length polypeptide PAG precursors (in silico translated from cloned cDNAs) identified in domestic cattle. Three other novel native isoforms (J1, J2 and K): EbUPG(45kDa) J1 (SKDNYKNYIPLIVPFAT), EbUPG(45kDa) J2 (SKDNQKNYIPLIVPFAT) and EbUPG(76kDa) K (SPEFTV), were temporarily named 'unknown placental glycoproteins' (UPGs), due to their efficient VVA-purification (specific for glycoproteins only) and a lack of considerable consensus to previously sequenced placental glycoproteins in the Bovidae family. This is the first study identifying NH(2)-terminals of multiple/diverse EbPAGs and some EbUPGs purified from the synepitheliochorial cotyledonary placenta of the endangered Bison bonasus (Red list) [less ▲]

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See detailIdentification of neurotensin-related peptides in human thymic epithelial cell membranes and relationship with MHC class I molecules
Vanneste, Yves; Ntodou-Thomé, Agnès; Vandersmissen, Eric et al

in Journal of Neuroimmunology (1997), 76

This study shows the expression at the cell surface of human thymic epithelial cells (TEC) of a neurotensin (NT)-like immunoreactivity. NT radio-immunoassay (RIA) revealed that cultured human TEC contain ... [more ▼]

This study shows the expression at the cell surface of human thymic epithelial cells (TEC) of a neurotensin (NT)-like immunoreactivity. NT radio-immunoassay (RIA) revealed that cultured human TEC contain ± 5 ng immunoreactive (ir) NT/106 cells, of which 5% is associated with plasma cell membranes. HPLC analysis of NT-ir present in human TEC showed a major peak of NT-ir corresponding to NT1–13. NT-ir was not detected in the supernatant of human TEC cultures. Using an affinity column prepared with a anti-MHC class I monoclonal antibody, NT-ir-related peptides were retained on the column and eluted together with MHC class I-related proteins. According to the elution time on HPLC of these peptides, they correspond to intact NT1–13, as well as to smaller fragments of NT1–13. [less ▲]

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See detailIdentification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis.
Conrotto, Paolo; Roesli, Cristof; Rybak, Jascha et al

in International Journal of Cancer = Journal International du Cancer (2008), 123(12), 2856-2864

One of the most promising new strategies for the development of efficacious cancer therapies relies on the targeted delivery of biopharmaceutical to the tumor environment by the use of selective and ... [more ▼]

One of the most promising new strategies for the development of efficacious cancer therapies relies on the targeted delivery of biopharmaceutical to the tumor environment by the use of selective and specific antibodies. The identification of accessible perivascular proteins selectively overexpressed in cancer tissue may facilitate the development of antibody-based biopharmaceutical administration. This approach is potentially highly selective and specific, combining the presence of tumor biomarkers readily accessible from the blood vessels and the high rate of angiogenesis characteristic of cancer tissues. We performed ex vivo perfusions of surgically resected human colon cancer using a reactive ester derivative of biotin, thus achieving a selective covalent modification of accessible proteins in vascular structures and stroma. After extraction and purification, biotinylated proteins were digested and the resulting peptides submitted to a comparative mass spectrometry-based proteomic analysis, revealing quantitative differences between normal and cancer colon. Sixty-seven of the total 367 proteins identified were found to be preferentially expressed at the tumor site. We generated human monoclonal antibodies against 2 potential tumor targets, NGAL and GW112, and we proved their selective expression in cancer colon and not or barely in healthy tissues. This article presents the first proteomic analysis of human colorectal cancer structures readily accessible from the tumor vasculature, revealing the overexpression of novel tumor antigens which may serve as selective targets for antibody-based imaging and therapeutic biomolecular strategies. (c) 2008 Wiley-Liss, Inc. [less ▲]

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See detailIdentification of new emerin partners in ceramide signalling pathway
DEROYER, Céline ULg; Renert, Anne-Françoise; Merville, Marie-Paule ULg et al

Conference (2012, May 25)

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See detailIdentification of new microbial enzymes from forest and marine ecosystems by functional metagenomics
Martin, Marjolaine ULg; Biver, Sophie ULg; Barbeyron, Tristan et al

Poster (2012, August 21)

Detailed reference viewed: 111 (36 ULg)
See detailidentification of new parterns of the NOD2 protein
Lecat, Aurore ULg; Di Valentin, Emmanuel ULg; Fillet, Marianne ULg et al

Poster (2011, January 27)

Detailed reference viewed: 28 (13 ULg)
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See detailIdentification of Non-Linear Dynamic Systems using an Expert Approach
Vio, Gareth Arthur; Dimitriadis, Grigorios ULg; Cooper, Jonathan Edward

in Proceedings of the 47th AIAA/ASME/ASCE/AHS/ASC Structures, Structural Dynamics, and Materials Conference (2006, May)

An Expert System approach for the identification of non-linear systems is presented. The Expert System is an attempt to bring order into the non-linear system identification process. The final objective ... [more ▼]

An Expert System approach for the identification of non-linear systems is presented. The Expert System is an attempt to bring order into the non-linear system identification process. The final objective is to deliver a parsimonious mathematical model of the dy- namical system under investigation. It works by defining the key stages of the procedure and iterating between them if necessary. It takes advantage of numerous methodologies to accomplish the tasks in each of the stages and uses the results from many of them. The Expert System applies the excitation forces appropriate to each method and analyses the responses. Each stage ends with a set of recommendations that can be used to begin the next stage. The Expert System is applied to a simple non-linear dynamic system. It is shown that the Expert System procedure can automatically detect, locate and quantify the non- linearity using its array of techniques. Furthermore, it can choose a suitable model struc- ture, select appropriate terms and estimate the model parameters using an extensive set of rules. The resulting identified model is validated and shown to be an accurate represen- tation of the experimental system. [less ▲]

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See detailIdentification of non-stationary dynamical systems using multivariate ARMA models
Bertha, Mathieu ULg; Golinval, Jean-Claude ULg

in Mechanical Systems and Signal Processing (2017), 88

This paper is concerned by the modal identification of time-varying mechanical systems. Based on previous works about autoregressive moving average models in vector form (ARMAV) for the modal ... [more ▼]

This paper is concerned by the modal identification of time-varying mechanical systems. Based on previous works about autoregressive moving average models in vector form (ARMAV) for the modal identification of linear time invariant systems, and time-varying autoregressive moving average models (TV-ARMA) for the identification of nonstationary systems, a time-varying ARMAV (TV-ARMAV) model is presented for the multivariate identification of time-varying systems. It results in the identification of not only the time-varying poles of the system but also of its respective time-varying mode shapes. The method is applied on a time-varying structure composed of a beam on which a mass is moving. [less ▲]

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See detailIdentification of Nonlinear Aeroelastic Systems
Dimitriadis, Grigorios ULg; Cooper, J. E.

in Proceedings of the 1998 International Conference on Noise and Vibration Engineering (1998, September)

The successful identification of nonlinear systems could find uses in many aerospace applications including the validation of finite element models and tracking the stability of aircraft during flight ... [more ▼]

The successful identification of nonlinear systems could find uses in many aerospace applications including the validation of finite element models and tracking the stability of aircraft during flight flutter testing. The effectiveness of existing nonlinear system identification techniques is limited by various factors such as the complexity of the system under investigation and the type of nonlinearities present. In this work, a new approach is introduced that is able to identify multi degree-of-freedom systems featuring any type of nonlinear function, including discontinuous functions. The method is shown to yield accurate identifications of three different simulated aeroelastic systems containing a wide range of structural nonlinearities. [less ▲]

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