Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailIn vitro entomopathogenic activity of Beauveria bassiana against Psoroptes spp. (Acari : Psoroptidae)
Lekimme, Mireille ULg; Mignon, Bernard ULg; Tombeux, S. et al

in Veterinary Parasitology (2006), 139(1-3), 196-202

An indigenous strain (IHEM 18747) of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes) was evaluated for its in vitro entomopathogenic activity against the parasitic mite Psoroptes ovis Hering ... [more ▼]

An indigenous strain (IHEM 18747) of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes) was evaluated for its in vitro entomopathogenic activity against the parasitic mite Psoroptes ovis Hering (Acari: Psoroptidae) from rabbits. The following aspects were evaluated: (1) effects of conidial concentration on the viability of adult females; (2) influence of the infection on the fertility, and on the hatchability of eggs; (3) and transmission of infection between mites, and from contaminated surface. Adult females immersed into increasing concentrations of conidia (10(4)-10(9) conidia ml(-1)) showed a dose-related susceptibility. At the highest concentration of conidia, LT50 was 1.6 days while LT50 of the controls reached 5.8 days. The fungus was able to sporulate on the body surface and 100% of the mites were covered with mycelium after immersion in solutions containing 10(7)-10(9) conidia ml(-1). One hundred percent of healthy mites exposed to infected cadavers or surfaces acquired the infection (LT50 reached 1.9 and 1.73 days, respectively, versus 6.1 and 5.1 days in controls, respectively). Egg laying was not reduced by the fungal infection but both the hatchability of the eggs and the life span of the emerging larvae were significantly reduced. Eggs directly infected with the fungus did not show reduced hatchability but the life span of the larvae was shortened. It is concluded that B. bassiana has a high entomopathogenic activity against Psoroptes spp. The in vivo use of this biocontrol agent against Psoroptes spp. in rabbit, sheep and cattle deserves further attention. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 81 (12 ULg)
Full Text
See detailIn vitro ESR measurements: powerful tool to study toxic effects on cells
Grammenos, Angeliki ULg; Quoilin, Caroline ULg; Guelluy, Pierre-Henri ULg et al

Poster (2011, October)

Electron spin resonance spectroscopy (ESR) is a highly efficient technique able to access a wide range of information about the unfavourable effects caused by a chemical or a drug. ESR in spin labelling ... [more ▼]

Electron spin resonance spectroscopy (ESR) is a highly efficient technique able to access a wide range of information about the unfavourable effects caused by a chemical or a drug. ESR in spin labelling fits well in with the study of membranes and particularly with the changes in lipid bilayer organisation induced by drug. Our team previously developed a way to quantify the effective lipid bilayer microviscosity of cell membranes and consequently put in evidence the fluidity effect of the propofol. Recently, the importance of lipid raft domains has been shown due to their important role as a platform for signal transduction and protein sorting. We propose to highlight the effect of the Randomly methylated beta cyclodextrin (Rameb) on these domains on membrane model (liposomes) as well as on colon carcinoma cell line (HCT-116). Futhermore, ESR in spin trapping is used in order to identify and quantify the generation of Reactive Oxygen Species (ROS) in cells. An ESR study on human colon carcinoma cell line has highlighted the cytotoxicity of the photosensitizer pyrophephorbide-a methyl ester. Using an intracellular located spin trap (4-pyridyl 1-oxide-N-tert-butylnitrone, POBN), it has been shown that the photoexcitation of the dye is able to generate superoxide anions, hydroxyl radicals and singlet oxygen. Moreover, ESR is one of the most sensitive method for measuring cellular oxygen consumption. Our team has studied the alterations of oxygen respiratory in human tubular renal cells treated with an endotoxin (lipopolysaccharide, LPS). The incubation of HK-2 cells with LPS elicited a decreased in oxygen consumption suggesting a down-regulation of the cells metabolism. [less ▲]

Detailed reference viewed: 52 (14 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of an anti-apoptotic drug, Z-VAD-FMK, for further use in ovarian tissue transplantation
Fransolet, Maïté ULg; HENRY, Laurie ULg; LABIED, Soraya ULg et al

Poster (2015, June 14)

Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results ... [more ▼]

Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results obtained with HGL5 granulosa cell line suggest that Z-VAD-FMK is efficient to protect granulosa cells from etoposide or CoCl2 induced apoptosis. What is known already: Removal, cryopreservation and subsequent graft of ovarian strips after cancer treatment have been successfully used to re-establish female fertility. However, the pregnancy rate after autografting of cryopreserved tissue is about 30%. Indeed, the major problem after transplantation is follicular loss due to ischemic reperfusion injury. Study design, size, duration: Three human granulosa cell lines (GC1a, HGL5 and COV434) were cultured during 48h with Z-VAD-FMK with or without etoposide to induce apoptosis. To reproduce the ischemic phase of the graft, cells were cultured without serum under reduced O2 (1%) or with CoCl2 (chemical hypoxia). Participants/materials, setting, methods: Granulosa cells were used as a model since they are essential for oocyte survival. Metabolic cell activity was evaluated by the WST-1 assay. Cell apoptosis was analyzed by flow cytometry after annexin V-FITC and propidium iodide double staining. The mRNA levels and protein expression of apoptotic markers were evaluated using RT-qPCR and western blot analysis. Main results and the role of chance: Flow cytometry showed that cells co-treated with etoposide and Z-VAD-FMK displayed a higher percentage of viable cells as compared to etoposide alone. When in vivo ischemic stage was mimicked (1% O2), no beneficial effect of the Z-VAD-FMK was detected. However, a significant decrease of the number of early apoptotic cells was evidenced by flow cytometry for HGL5 cells treated with Z-VAD-FMK. RT-qPCR and western blot analysis revealed that apoptotic molecules were not modulated. Metabolic activity of the 3 cell lines was reduced by CoCl2. For HGL5 cells, this decrease was partially reversed by Z-VAD-FMK. The number of viable cells was reduced by CoCl2 in HGL5 cells but Z-VAD-FMK allowed to preserve a similar number of viable and apoptotic cells than in control condition. Limitations, reasons for caution: In this study we used 3 different cell lines but granulosa cells represent only a part of the cell types present in ovarian tissue biopsies. Experiences on the effect of Z-VAD-FMK on primary culture of granulosa cells have not yet been realized. Wider implications of the findings: This study suggests that the use of an antiapoptotic drug could be efficient to improve ovarian tissue transplantation outcomes. Ovarian tissue grafting studies using our xenograft murine model will be performed to test the potential efficacy of this drug to improve tissue viability and primordial follicles preservation after transplantation. [less ▲]

Detailed reference viewed: 63 (14 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of antiplasmodial activity of plant samples used in traditional medicine in Benin
Ganfon, H.; Gbaguidi, F.; Frederich, Michel ULg et al

in Planta Medica (2008), 74(9), 1140-1140

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of fermentation characteristics of two types of insects, as potential novel protein feeds for pigs
POELAERT, Christine ULg; Beckers, Yves ULg; Despret, Xavier et al

in 13th International Symposium on Digestive Physiology of Pigs (2015, May)

Detailed reference viewed: 88 (23 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of glutathione peroxidase (GPx)-like activity and antioxidant properties of some Ebselen analogues
Mouithys-Mickalad, Ange ULg; Mareque-Faez, Juan; Chistiaens, U. et al

in Redox Report : Communications in Free Radical Research (2004), 9(2), 81-87

Four analogues of Ebselen were synthesized and their glutathione peroxidase activity and antioxidant property evaluated and compared to Ebselen. Among the studied compounds, only diselenide [3] exhibited ... [more ▼]

Four analogues of Ebselen were synthesized and their glutathione peroxidase activity and antioxidant property evaluated and compared to Ebselen. Among the studied compounds, only diselenide [3] exhibited both glutathione peroxidase activity and radical-scavenging capability. Compounds [3] and [4] showed a strong inhibitory effect (53% and 43%, respectively) on the lipid peroxidation of linoleic acid compared to Ebselen and selenide derivatives ([1] and [2]) which were less active (28%, 26% and 18% inhibition, respectively). A concentration-dependent inhibitory effect was also found in the model of the formation of ABTS*+ radical cation: 65% and 89% inhibition for compound [3] at 10(-4) M and 5 x 10(-5) M, respectively, and 68% and 90% for compound [4], compared to 14% and 52% inhibition for Ebselen and the diselenides [1] and [2] (29%, 46% and 45%, 68%, respectively). By EPR spin trapping technique, the following inhibitory profile of the Ebselen analogues was observed towards the formation of thiyl radicals: Ebselen = [3]>[1]>[2]>[4]. Studies with compound [3] are in progress on oxidative stress cell models. [less ▲]

Detailed reference viewed: 34 (2 ULg)
Full Text
Peer Reviewed
See detailAn in vitro evaluation of leakage of two Etch and Rinse and two Self-Etch adhesives after thermocycling
Geerts, Sabine ULg; BOLETTE, Amandine ULg; SEIDEL, Laurence ULg et al

in International Journal of Dentistry (2012)

Our experiment evaluated the microleakage in resin composite restorations bonded to dental tissues with different adhesive systems. 40 class V cavities were prepared on the facial and lingual surfaces of ... [more ▼]

Our experiment evaluated the microleakage in resin composite restorations bonded to dental tissues with different adhesive systems. 40 class V cavities were prepared on the facial and lingual surfaces of each tooth with coronal margins in enamel and apical margins in cementum (root dentin). The teeth were restored with Z100 resin composite bonded with different adhesive systems: Scotchbond! Multipurpose (SBMP) a 3-step Etch and Rinse adhesive, Adper! Scotchbond! 1 XT (SB1) a 2-step Etch and Rinse adhesive, AdheSE® One (ADSE-1) a 1-step Self-Etch adhesive and AdheSE® (ADSE) a 2-step Self-Etch adhesive. Teeth were thermocycled and immersed in 50 % silver nitrate solution. When both interfaces were considered, SBMP has exhibited significantly less microleakage than other adhesive systems (respectively for SB1, ADSE-1 and ADSE, p = 0.0007, p < 0.0001 and p < 0.0001). When enamel and dentin interfaces were evaluated separately: 1) for the Self-Etch adhesives, microleakage was found greater at enamel than at dentin interfaces (for ADSE, p = 0.024 and for ADSE-1, p < 0.0001); 2) for the Etch and Rinse adhesive systems, there was no significant difference between enamel and dentin interfaces; 3) SBMP was found significantly better than other adhesives both at enamel and dentin interfaces. In our experiment Etch and Rinse adhesives remain better than Self-Etch adhesives at enamel interface. In addition, there was no statistical difference between 1-step (ADSE-1) and 2-step (ADSE) Self-Etch adhesives. [less ▲]

Detailed reference viewed: 83 (8 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of lipid matrices for the development of a sustained-release sulfamethazine bolus for lambs
Evrard, Brigitte ULg; Delattre, Luc ULg

in Drug Development & Industrial Pharmacy (1996), 22(2), 111-118

The aim of this study is the development of a high-density bolus to be given to lambs in order to release sulfamethazine during 4 days after a single oral administration. The suitability of 12 lipid ... [more ▼]

The aim of this study is the development of a high-density bolus to be given to lambs in order to release sulfamethazine during 4 days after a single oral administration. The suitability of 12 lipid matrix formulations was assessed in order to maintain the integrity of the tablet even after complete dissolution of the active substance. The influence of both nature and concentration of the lipid excipient on the in vitro release of sulfamethazine was investigated. The influences of the granulation method and of the compression force were assessed on a formulation containing Cutina® HR as a binding and sustained-release agent. Finally, stability tests showed that the in vitro release characteristics remain unchanged during storage. [less ▲]

Detailed reference viewed: 42 (0 ULg)
Full Text
See detailIn vitro evaluation of protein precipitation capacity of temperate browse species
Vandermeulen, Sophie ULg; Leblois, Julie ULg; Ramírez-Restrepo, Carlos Alberto et al

Poster (2016, February 05)

European agri-environmental policies are promoting the establishment of shrubs and trees on grasslands. The use of browse as fodder requires knowledge on their nutritive value since intensive production ... [more ▼]

European agri-environmental policies are promoting the establishment of shrubs and trees on grasslands. The use of browse as fodder requires knowledge on their nutritive value since intensive production systems are still relying on expensive and environment-costing protein sources. However, information on the influence of temperate condensed tannins (CT)-containing browse forage on rumen protein metabolism is elusive. The study aimed to assess the protein precipitation capacity (PPC) of 10 temperate browse species and establish the correlation between PPC values and plants CT content. PPC of foliage of 3 individuals per woody plants was measured using 2 model proteins: bovine serum albumin (BSA) and casein. The N content in protein solutions (4.6g/L; pH=6.8) was determined before and after adding each forage sample. Extractable CT concentration was quantified by spectrophotometry. The PPC varied across plant species (P<0.001). Corylus avellana had the highest ability to precipitate casein (52.4%). In contrast, the BSA precipitation (18.3%) of this plant was similar to Cornus sanguinea (12.7%), Quercus robur (12.1%) and Crataegus monogyna (11.0%). CT content ranged from 1.4 in Fraxinus excelsior to 82.7g/kg of depigmented sample in Corylus avellana (P<0.001) and was correlated to BSA (r=0.70; P<0.001) and casein PC (r=0.51; P<0.01). It was concluded that woody species could play a significant role in modifying protein metabolism, but further in vivo trials are required. [less ▲]

Detailed reference viewed: 43 (9 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of the anti-apoptotic drug Z-VAD-FMK on human ovarian granulosa cell lines for further use in ovarian tissue transplantation.
Fransolet, Maïté ULg; HENRY, Laurie ULg; Labied, Soraya et al

in Journal of Assisted Reproduction & Genetics (2015)

PURPOSE: Because ovarian granulosa cells are essential for oocyte survival, we examined three human granulosa cell lines as models to evaluate the ability of the pan-caspase inhibitor benzyloxycarbonyl ... [more ▼]

PURPOSE: Because ovarian granulosa cells are essential for oocyte survival, we examined three human granulosa cell lines as models to evaluate the ability of the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK) to prevent primordial follicle loss after ovarian tissue transplantation. METHODS: To validate the efficacy of Z-VAD-FMK, three human granulosa cell lines (GC1a, HGL5, COV434) were treated for 48 h with etoposide (50 mug/ml) and/or Z-VAD-FMK (50 muM) under normoxic conditions. To mimic the ischemic phase that occurs after ovarian fragment transplantation, cells were cultured without serum under hypoxia (1 % O2) and treated with Z-VAD-FMK. The metabolic activity of the cells was evaluated by WST-1 assay. Cell viability was determined by FACS analyses. The expression of apoptosis-related molecules was assessed by RT-qPCR and Western blot analyses. RESULTS: Our assessment of metabolic activity and FACS analyses in the normoxic experiments indicate that Z-VAD-FMK protects granulosa cells from etoposide-induced cell death. When cells are exposed to hypoxia and serum starvation, their metabolic activity is reduced. However, Z-VAD-FMK does not provide a protective effect. In the hypoxic experiments, the number of viable cells was not modulated, and we did not observe any modifications in the expressions of apoptosis-related molecules (p53, Bax, Bcl-xl, and poly (ADP-ribose) polymerase (PARP)). CONCLUSION: The death of granulosa cell lines was not induced in our ischemic model. Therefore, a protective effect of Z-VAD-FMK in vitro for further use in ovarian tissue transplantation could not be directly confirmed. It will be of interest to potentially use Z-VAD-FMK in vivo in xenograft models. [less ▲]

Detailed reference viewed: 41 (5 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of the fermentation characteristics in the pig intestines of hulless barleys differing in β-glucan content
Jha, Rajesh; Bindelle, Jérôme ULg; Rossnagel, Brian et al

in Journal of Animal Science (2009), 87(E-Suppl. 3), 103

Non-starch polysaccharides (NSP) in isolated form, especially β-glucans, are reported to have prebiotic effects in pigs. However, little information is available on the possible functional properties of ... [more ▼]

Non-starch polysaccharides (NSP) in isolated form, especially β-glucans, are reported to have prebiotic effects in pigs. However, little information is available on the possible functional properties of these NSP when the latter are still present in the fibrous matrix of whole cereals. Hulless barleys (HB) are good sources of β-glucans and the content is quite variable among varieties. In order to evaluate the potential of HB as functional feeds, an in vitro experiment was carried out to study the fermentation characteristics of 6 HB varieties varying in their β-glucan contents (36-99 g/kg DM) in comparison to 3 hulled barleys and 5 oats. After a pepsin-pancreatin hydrolysis, the ingredients were incubated in a buffer solution containing minerals and pig feces as inoculum. The accumulated gas production, proportional to the amount of fiber fermented, was measured for 48 h and modeled. Short-chain fatty acid (SCFA) and ammonia concentration were measured in the fermented solutions. A cereal type effect (P < 0.05) was observed on the fermentation kinetics parameters. Rates of degradation and total gas productions were higher in HB than in oats (P < 0.05) but no difference was observed between HB varieties. On the contrary, differences were found between HB for lag time and rate of degradation. The production of SCFA was also higher with HB (6.1 mMol/g DM incubated; P < 0.05) than with hulled barleys and oats (4.9 and 2.9 mMol/g DM incubated respectively). In contrast, oats generated higher ammonia (P <0.05) production (1.4 mMol/g DM incubated, on average) than barley (1.0 mMol/g). In conclusion, HB are better fermented in vitro, produce more beneficial (SCFA) and less harmful (ammonia) metabolites and have a better potential than other cereal species to modulate gut microbiota and improve gut health. [less ▲]

Detailed reference viewed: 190 (6 ULg)
Full Text
Peer Reviewed
See detailIn vitro evaluation of the fermentation characteristics of the carbohydrate fractions of hulless barley and other cereals in the gastrointestinal tract of pigs
Jha, Rajesh; Bindelle, Jérôme ULg; Rossnagel, Brian et al

in Animal Feed Science & Technology (2011), 163

An in vitro model was used to study the fermentation characteristics of carbohydrate fractions of hulless barley (hB), in comparison to hulled barley (HB), hulled oat and oat groats (OG) in the pig ... [more ▼]

An in vitro model was used to study the fermentation characteristics of carbohydrate fractions of hulless barley (hB), in comparison to hulled barley (HB), hulled oat and oat groats (OG) in the pig intestine. For this purpose, 6 hulless barley cultivars (hB), varying in β-glucan content (36-99 g/kg DM), were compared to 3 HB cultivars, 2 oat groat samples (OG), 3 oat varieties and a reference sample of wheat. The residue of a pepsin-pancreatin hydrolysis was incubated in a buffered mineral solution inoculated with pig faeces. Gas production, proportional to the amount of fermented carbohydrates, was measured for 48 h and kinetics modelled. The fermented solution was subsequently analyzed for microbial production of short-chain fatty acids (SCFA) and ammonia. In vitro dry matter degradability varied according to ingredient (P<0.001). Higher values were observed for OG, ranging from 0.88 to 0.99 as compared to oat, hB and HB, for which degradability ranged from 0.63 to 0.73, 0.68 to 0.80 and 0.69 to 0.71, respectively. A “cereal type” effect (P<0.05) was observed on fermentation kinetics parameters. Total gas production was higher (P<0.05) with hB (224 ml/g DM incubated) than with HB and oat (188 and 55 ml/g DM incubated, respectively). No difference was observed between hB cultivars (P>0.05) for total gas production but differences (P<0.001) were found for lag time and the fractional rate of degradation. Hulless barley cultivar CDC Fibar (waxy starch) and CDC McGwire (normal starch) started to ferment sooner (lag time of 0.7 and 0.9 h, respectively) than SH99250 (high amylose starch; 1.7 h). The fractional rate of degradation was similar in both hB and OG (0.15/h on average), which was higher than that of HB (0.12/h). The production of SCFA was also higher (P<0.05) with hB (6.1 mmol/g DM incubated, on average) than with HB and oat (4.9 and 2.9 mmol/g DM incubated, respectively). Similar trends were found for SCFA production expressed per g fermented carbohydrates, with higher butyrate and lower acetate ratio. In contrast, oat fermentation generated higher (P<0.05) ammonia concentration (1.4 mmol/g DM incubated, on average) than hB (1.0 mmol/g DM incubated). In summary, hulless barleys, irrespective of cultivar type had higher in vitro fermentability and produced more SCFA and less ammonia than hulled barley and oat. Thus, hulless barleys have a better potential to be used in pig nutrition to manipulate the fermentation activity in the intestine of pigs. [less ▲]

Detailed reference viewed: 65 (9 ULg)
See detailIn vitro expression of RC2-labeled antigens as radial glial cells markers in cultures derived from mouse embryonic brain
Leprince, P; Chanas-Sacré, G; Lewin, M et al

Poster (1996)

Detailed reference viewed: 9 (0 ULg)