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See detailIdentification of tenuis of four French Polynesian Carapini (Carapidae : Teleostei)
Parmentier, Eric ULg; Lo-Yat, A.; Vandewalle, Pierre ULg

in Marine Biology (2002), 140(3), 633-638

Four species of adult Carapini (Carapidae) occur on Polynesian coral reefs: Encheliophis gracilis, Carapus boraborensis, C. homei and C. mourlani. Samples collected in Rangiroa and Moorea allowed us to ... [more ▼]

Four species of adult Carapini (Carapidae) occur on Polynesian coral reefs: Encheliophis gracilis, Carapus boraborensis, C. homei and C. mourlani. Samples collected in Rangiroa and Moorea allowed us to obtain different tenuis (larvae) during their settlement phases or directly inside their hosts. These were separated into four lots on the basis of a combination of pigmentation, meristic, morphological, dental and otolith (sagittae) features. Comparison of these characters with those of the adults allows, for the first time, taxonomic identification of these tenuis-stage larvae. [less ▲]

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See detailIdentification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein.
Clausse, Nathalie; Jackers, Pascale ULg; Jares, P. et al

in DNA & Cell Biology (1996), 15(12), 1009-23

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents ... [more ▼]

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents intriguing multifunctional properties because it has been characterized as the precursor of the metastasis-associated 67-kD laminin receptor (67LR) and as a cytoplasmic ribosomal-associated protein. The isolation of the 37LRP/p40 gene is a prerequisite for identifying the molecular mechanisms responsible for the constant up-regulation of the 67LR expression in cancer cells. To date, the active 37LRP/p40 gene has never been identified in any species due to the existence of multiple pseudogenes in most vertebrates genomes. In this study, we report for the first time the gene structure and potential regulatory sequences of the 37 LRP/p40 gene. The chicken genome was selected to undergo this characterization because it is the only known vertebrate that bears a single 37 LRP/p40 gene copy. The 37 LRP/p40 active gene is composed of 7 exons and 6 introns and bears features characteristic of a ribosomal protein gene. It does not bear a classical TATA box and it exhibits several transcription initiation sites as demonstrated by RNase protection assay and primer extension. Analysis of potential regulatory regions suggests that gene expression is driven not only by the 5' genomic region but also by the 5' untranslated and intron 1 sequences. On the basis of gene structure and extensive protein evolutionary study, we found that the carboxyterminal domain of the protein is a conserved lock-and-key structure/function domain that could be involved in the biosynthesis of the higher-molecular-weight 67-kD laminin receptor in vertebrates, whereas the central core of the protein would be responsible for the ribosome associated function. The first identification of the active 37LRP/p40 gene presented in this study is a critical step toward the isolation of the corresponding human gene and the understanding of the molecular mechanisms involved in the up-regulation of its expression during tumor invasion and metastasis. [less ▲]

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See detailIdentification of the bacterial symbionts of the banana aphid Pentalonia nigronervosa and characterization of their roles
De Clerck, Caroline ULg

Doctoral thesis (2015)

Les insectes sont caractérisés par une incroyable capacité d’adaptation à de nombreux environnements et modes d’alimentation, qui est liée au fait que la majorité des insectes (voire tous) hébergent des ... [more ▼]

Les insectes sont caractérisés par une incroyable capacité d’adaptation à de nombreux environnements et modes d’alimentation, qui est liée au fait que la majorité des insectes (voire tous) hébergent des symbiontes intra ou extracellulaires. Ces associations symbiotiques sont très diverses et peuvent aller d’un mutualisme obligatoire à un parasitisme facultatif. Les pucerons, en particulier, sont connus pour vivre en symbiose avec au moins une bactérie obligatoire qui leur fournit les nutriments essentiels absents du phloème dont ils se nourrissent : Buchnera aphidicola. La plupart des symbiontes d’insectes étant incultivables, les technologies moléculaires modernes se sont avérées des outils puissants dans la compréhension des interactions hôte-microorganisme et la caractérisation du rôle joués par ces symbiontes. Les études génomiques ont mené à la découverte des plus petits génomes bactériens jamais observés, et ont permis une étude plus poussée des symbiontes secondaires et de leur évolution. Pentalonia nigronervosa, le puceron noir du bananier, lest le vecteur principal du virus des sommets touffus du bananier, l’un des virus les plus dommageables en culture bananière. Très peu d’études sont néanmoins disponibles sur ce vecteur et rien n’était connu sur la population symbiotique hébergée par cet insecte jusqu’à cette étude. Dans cette thèse, nous avons découvert que le puceron du bananier contient deux symbiontes : B. aphidicola (BPn) et Wolbachia sp. (wPn); et cela dans tous les pucerons testés, quelle que soit leur origine géographique. La présence de Buchnera était attendue (99,9% de toutes les espèces de pucerons contiennent ce symbionte), mais la détection systématique de Wolbachia nous fait nous interroger sur le rôle que cette bactérie pourrait jouer pour l’insecte hôte, ainsi que sur la raison de sa présence. Wolbachia n’est en effet que rarement détectée chez les pucerons et est surtout connue pour être un parasite qui manipule le système reproducteur de ces hôtes. Malgré tout, des études ont montré que cette bactérie pouvait agir comme mutualiste pour certains insectes, ce qui pourrait expliquer sa présence dans le puceron du bananier. Le second objectif de cette étude a donc été de mieux comprendre le rôle joué par les deux symbiontes de P. nigronervosa. Pour ce faire, différentes techniques complémentaires ont été utilisées. La microinjection d’antibiotiques a été utilisée afin d’éliminer sélectivement wPn et de voir l’effet de cette élimination sur l’hôte. Nous avons observé que l’élimination de wPn donnait systématiquement lieu à la mort du puceron. Un séquençage haut débit de l’hémolymphe (contenant des bacteriocytes) d’un puceron a été réalisé et nous a permis d’identifier et d’annoter 587 et 250 gènes pour wPn et BPn respectivement. L’analyse de ces gènes nous permet d’émettre l’hypothèse que les deux bactéries collaborent pour la production de plusieurs nutriments essentiels pour l’hôte. La situation est surtout frappante dans le cas de la lysine et de la riboflavine, qui sont habituellement produits uniquement par Buchnera chez les autres espèces de pucerons. Dans le cas du puceron du bananier, les gènes impliqués dans les voies de production de ces métabolites sont répartis entre les génomes des deux bactéries, les rendant ainsi toutes les deux essentielles à la survie du puceron hôte. Enfin, une localisation par fluorescence in situ des deux symbiontes a montré qu’ils étaient tous deux localisés dans les bacteriocytes, rendant les échanges de précurseurs et métabolites possibles. En conclusion, nos résultats suggèrent que le puceron du bananier héberge une co-symbiose obligatoire entre B. aphidicola et Wolbachia, les deux bactéries agissant de concert dans l’approvisionnement de l’hôte en nutriments essentiels. [less ▲]

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See detailIdentification of the bovine leukemia virus transactivating protein (p34x).
Willems, Luc ULg; Kettmann, Richard ULg; Portetelle, Daniel ULg et al

in Haematology and Blood Transfusion (1987), 31(1), 482-7

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See detailIdentification of the cis element recognized by a transcription factor specific to the tumor BT-474 mammary cells.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Hodzic, Didier et al

Conference (1996, February 03)

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See detailIdentification of the concepts at stake during a consultation. Clinical exercises
Jamoulle, Marc ULg

E-print/Working paper (2015)

During a consultation between a patient and a doctor, symptoms, process and health problems are at stake and could be identified and retrieved through the use of ICPC.(refer to www.ph3c.org) Specific ... [more ▼]

During a consultation between a patient and a doctor, symptoms, process and health problems are at stake and could be identified and retrieved through the use of ICPC.(refer to www.ph3c.org) Specific training and managerial competences of the doctor could be identified and explained through the Q-Codes Together ICPC and Q-Codes form 3CGP ; Core Content Classification in General Practice/ Family Medicine The concepts identified in ICPC and Q-Codes could be useful to understand and teach the content of consultation in family medicine. [less ▲]

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See detailIdentification of the Core Structure of Lysozyme Amyloid Fibrils by Proteolysis
Frare, Erica; Mossuto, Maria F.; Polverino de Laureto, Patrizia et al

in Journal of Molecular Biology (2006), 361

Human lysozyme variants form amyloid fibrils in individuals suffering from a familial non-neuropathic systemic amyloidosis. In vitro, wild-type human and hen lysozyme, and the amyloidogenic mutants can be ... [more ▼]

Human lysozyme variants form amyloid fibrils in individuals suffering from a familial non-neuropathic systemic amyloidosis. In vitro, wild-type human and hen lysozyme, and the amyloidogenic mutants can be induced to form amyloid fibrils when incubated under appropriate conditions. In this study, fibrils of wild-type human lysozyme formed at low pH have been analyzed by a combination of limited proteolysis and Fouriertransform infrared (FTIR) spectroscopy, in order to map conformational features of the 130 residue chain of lysozyme when embedded in the amyloid aggregates. After digestion with pepsin at low pH, the lysozyme fibrils were found to be composed primarily of N and C-terminally truncated protein species encompassing residues 26–123 and 32–108, although a significant minority of molecules was found to be completely resistant to proteolysis under these conditions. FTIR spectra provide evidence that lysozyme fibrils contain extensive β-sheet structure and a substantial element of non β-sheet or random structure that is reduced significantly in the fibrils after digestion. The sequence 32–108 includes the β-sheet and helix C of the native protein, previously found to be prone to unfold locally in human lysozyme and its pathogenic variants. Moreover, this core structure of the lysozyme fibrils encompasses the highly aggregation-prone region of the sequence recently identified in hen lysozyme. The present proteolytic data indicate that the region of the lysozyme molecule that unfolds and aggregates most readily corresponds to the most highly protease-resistant and thus highly structured region of the majority of mature amyloid fibrils. Overall, the data show that amyloid formation does not require the participation of the entire lysozyme chain. The majority of amyloid fibrils formed from lysozyme under the conditions used here contain a core structure involving some 50% of the polypeptide chain that is flanked by proteolytically accessible N and C-terminal regions. [less ▲]

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See detailIdentification of the dairy cows treated with bovine somatotropine (BST): criteria to discriminate the treated animals from the non-treated ones
Scippo, Marie-Louise ULg; Degand, Guy ULg; Maghuin-Rogister, Guy ULg

in Second meeting on animal productions. The milk channel], Centre de Recherches Agronomiques, Gembloux (Belgium); Faculte Universitaire des Sciences Agronomiques de Gembloux (Belgium).- Gembloux (Belgium), 1997. p. Q3 (1997)

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See detailIdentification of the different sources responsible for olfactory annoyance, using an e-nose
clarke, Kevin; Redon, Nathalie; Locoge, Nadine et al

in Perena, Gouma, (Ed.) OLFACTION AND ELECTRONIC NOSE: PROCEEDINGS OF THE 14TH INTERNATIONAL SYMPOSIUM ON OLFACTION AND ELECTRONIC NOSE (2011, May)

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See detailIdentification of the linear parts of nonlinear systems for fuzzy modeling
Rezaei Sadrabadi, Mahmood ULg

in Applied Soft Computing (2011), 11

In direct approach to fuzzy modeling, structure identification is one of the most critical tasks. In modeling the nonlinear system, this fact is more crucial. In this paper, a new hybrid method is ... [more ▼]

In direct approach to fuzzy modeling, structure identification is one of the most critical tasks. In modeling the nonlinear system, this fact is more crucial. In this paper, a new hybrid method is proposed to cluster the data located in the linear parts on the nonlinear systems. The proposed method can partition the input–output data in two groups: data located in the linear parts and data in the extrema. It is shown that the first group of data is suitable to be clustered by Fuzzy C-Regression Model (FCRM) clustering algorithm and the second group by Fuzzy C-Means (FCM). Then, based on the above findings, a new hybrid clustering algorithm is proposed. Finally, the proposed approach is tested and validated by several numerical examples of nonlinear functions. [less ▲]

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See detailIdentification of the main parameters controlling the plasticity of ceramic pastes: The case study of the Marrakech region (Morocco)
El Boudour El Idrissi, Hicham ULg; Daoudi, Lahcen; Fontaine, François ULg et al

Poster (2015, July)

Numerous traditional ceramic workshops occur within a range of 10 to 80 km around the city of Marrakech. Our study aims to identify the main parameters controlling the plasticity of pastes used by the ... [more ▼]

Numerous traditional ceramic workshops occur within a range of 10 to 80 km around the city of Marrakech. Our study aims to identify the main parameters controlling the plasticity of pastes used by the artisans. To reach this goal, we characterize by X-ray diffraction, granulometry and plasticity index (PI) the local raw clayey material. <br />The investigation of 26 whole pastes shows the dominance of quartz (20 to 50%) and clay minerals (25 to 60%) with K-feldspar (2 to 17%), plagioclase (2 to 25%), calcite (0 to 18%), dolomite (0 to 15%), goethite (0 to7%) and trace of hematite and anhydrite (< 3%). Amphibole occurs in trace (< 5%) but only in some samples. The clay minerals are diversified, including illite (10 to 40%), kaolinite (2 to 15%), mixed-layer (ML) (0 to 10%), smectite, vermiculite and chlorite (0 to 5%), and pyrophyllite-talc association (0 to 8%). Sepiolite (12%) is only present in one paste. The grain size is made by variable proportions of sand (5 to 65%), silt (12 to 53%) and clayey fraction (18 to 66%). <br />The Plasticity index (PI) indicates the presence of two principals groups of pastes. <br />(1) The first group is characterized by PI values ranging between 15 to 18. Their moderate plasticity behavior is related either to the low rate of clayey fraction and/or to the absence of plastic clays like smectites and mixed layer with smectitic sheets. <br />(2) The PI of second group ranges between 20 to 32. The high plasticity values are influenced by the presence of specific clayey minerals like talc-pyrophyllite or sepiolite, or by the high content of smectite plus smectitic mixed-layers within the clayey (< 2 micron) fraction. Only one sample without any of these plastic minerals but with a high content of clayey fraction is characterized by a high PI value (24). <br />We concluded that the workability of ceramic paste in Marrakech region is controlled by a combination of factors, dominated by the grain size distribution and the content in plastic clay minerals within the fine fraction. [less ▲]

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See detailIdentification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family
Xie, Sancai; Low, Boon G.; Nagel, Robert J. et al

in Proceedings of the National Academy of Sciences of the United States of America (1991), 88(22), 10247-10251

Pregnancy in cattle and sheep can be diagnosed by the presence of a conceptus-derived antigen in maternal serum that is secreted by trophoblast and placental tissue primarily as an acidic component of Mr ... [more ▼]

Pregnancy in cattle and sheep can be diagnosed by the presence of a conceptus-derived antigen in maternal serum that is secreted by trophoblast and placental tissue primarily as an acidic component of Mr 67,000. Molecular cloning of its cDNA reveals that the antigen belongs to the aspartic proteinase family and has greater than 50% amino acid sequence identity to pepsin, cathepsin D, and cathepsin E. The inferred sequences of the ovine and bovine polypeptides show approximately 73% identity to each other. Critical amino acid substitutions at the active site regions suggest that both proteins are enzymatically inactive. The antigen is a product of trophoblast binucleate cells that invade maternal endometrium at implantation sites. [less ▲]

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See detailIdentification of the Mechanical and Piezoelectric Parameters of a Massively Piezoelectric Structure
De Boe, P.; Pascual, R.; Golinval, Jean-Claude ULg

Conference given outside the academic context (1998)

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See detailIdentification of the neutralizing epitopes of Merkel cell polyomavirus major capsid protein within the BC and EF surface loops.
Fleury, Maxime J. J.; Nicol, Jérôme ULg; Samimi, Mahtab et al

in PloS one (2015), 10(3), 0121751

Merkel cell polyomavirus (MCPyV) is the first polyomavirus clearly associated with a human cancer, i.e. the Merkel cell carcinoma (MCC). Polyomaviruses are small naked DNA viruses that induce a robust ... [more ▼]

Merkel cell polyomavirus (MCPyV) is the first polyomavirus clearly associated with a human cancer, i.e. the Merkel cell carcinoma (MCC). Polyomaviruses are small naked DNA viruses that induce a robust polyclonal antibody response against the major capsid protein (VP1). However, the polyomavirus VP1 capsid protein epitopes have not been identified to date. The aim of this study was to identify the neutralizing epitopes of the MCPyV capsid. For this goal, four VP1 mutants were generated by insertional mutagenesis in the BC, DE, EF and HI loops between amino acids 88-89, 150-151, 189-190, and 296-297, respectively. The reactivity of these mutants and wild-type VLPs was then investigated with anti-VP1 monoclonal antibodies and anti-MCPyV positive human sera. The findings together suggest that immunodominant conformational neutralizing epitopes are present at the surface of the MCPyV VLPs and are clustered within BC and EF loops. [less ▲]

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See detailIdentification of the nonlinear function in a vibrating nonlinear beam
Dimitriadis, Grigorios ULg; Cooper, Jonathan E

in Lees, A. W.; Prells, U.; Norton, J. (Eds.) Proceedings of the Third International Conference on Identification in Engineering Systems (2002, April)

The Constant Level Identification (CLI) method for nonlinear dynamical systems has been shown to identify accurately a wide variety of systems with discrete degrees of freedom. In this paper, the method ... [more ▼]

The Constant Level Identification (CLI) method for nonlinear dynamical systems has been shown to identify accurately a wide variety of systems with discrete degrees of freedom. In this paper, the method is applied to a continuous nonlinear system, namely a simulated model of a vibrating beam attached to a nonlinear spring. A Rayleigh-Ritz series representation of the beam is assumed in order to extract the desired number of modal responses. Then, a combination of modal and physical coordinates are used in the identification process. It is shown that the CLI method can accurately capture the shape of the nonlinearity in the system. [less ▲]

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See detailIdentification of the Origin of Catecholaminergic Inputs to Hvc in Canaries by Retrograde Tract Tracing Combined with Tyrosine Hydroxylase Immunocytochemistry
Appeltants, D.; Absil, Philippe ULg; Balthazart, Jacques ULg et al

in Journal of Chemical Neuroanatomy (2000), 18(3), 117-33

The telencephalic nucleus HVc (sometimes referred to as the high vocal center) plays a key role in the production and perception of birdsong. Although many afferent and efferent connections to this ... [more ▼]

The telencephalic nucleus HVc (sometimes referred to as the high vocal center) plays a key role in the production and perception of birdsong. Although many afferent and efferent connections to this nucleus have been described, it has been clear for many years, based on chemical neuroanatomical criteria, that there are projections to this nucleus that remain undescribed. A variety of methods including high performance liquid chromatography, immunohistochemistry and receptor autoradiography have identified high levels of catecholamine transmitters, the presence of enzymes involved in the synthesis of catecholamines such as tyrosine hydroxylase and a variety of catecholamine receptor sub-types in the HVc of several songbird species. However, no definitive projections to HVc have been described from cells groups known to synthesize catecholamines. These projections were analyzed in the present study by retrograde tract tracing combined with immunocytochemistry for tyrosine hydroxylase. The origin of the catecholaminergic inputs to HVc were determined based exclusively on birds in which injections of the retrograde tracer (latex fluospheres) were confined within the cytoarchitectonic boundaries of the nucleus. Retrogradely transported latex fluospheres were found mainly in cells of two dopaminergic nuclei, the mesencephalic central gray (A11) and, to a lesser extend, the area ventralis of Tsai (A10; homologous to the ventral tegmental area of mammals). A few retrogradely-labelled cells were also found in the noradrenergic nucleus subceruleus (A6). Most of these retrogradely-labelled cells were also tyrosine hydroxylase-positive. Other catecholaminergic nuclei were devoid of retrograde label. These data converge with others studies to indicate that HVc receives discrete dopaminergic and noradrenergic inputs. These inputs may influence the steroid regulation of HVc, attentional processes related to song and modulate sensory inputs to the song system. [less ▲]

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See detailIdentification of the parameters of a damage constitutive law for steel at elevated temperature
Castagne, S.; Habraken, Anne ULg

in Banabic, D. (Ed.) Proceedings of the 8th ESAFORM Conference on Material Forming (2005)

This paper presents the methodology and the results of the calibration of an interface damage law using experimental damage analyses and associated finite element simulations. The experimental damage ... [more ▼]

This paper presents the methodology and the results of the calibration of an interface damage law using experimental damage analyses and associated finite element simulations. The experimental damage analyses consist in acoustic tests realised on steel samples in order to determine the apparition of the first crack during compression. Finite elements simulations of these experiments allow the determination of the damage parameters using a reverse method. [less ▲]

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