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See detailIdentification of elemental sulphur (S8) in Boscia senegalensis (Pers.) Lam ex Poir. leaves.
Lognay, Georges ULg; Seck, D.; Marlier, M. et al

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(4),

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See detailIdentification of embryonic/fetal mortality in cows by semiquantitative detection of pregnancy-associated glycoproteins
Engelke, J; Knaack, H; Linden, M et al

in Livestock Science (2015), 178

Late embryonic and early fetal mortality (EM/FM) might occur following a pregnancy diagnosis, decreasing the accuracy of pregnancy diagnostics in dairy cattle. Therefore, the detection of EM/FM may be ... [more ▼]

Late embryonic and early fetal mortality (EM/FM) might occur following a pregnancy diagnosis, decreasing the accuracy of pregnancy diagnostics in dairy cattle. Therefore, the detection of EM/FM may be useful in dairy reproductive management. Comparatively low concentrations of pregnancy-associated glycoproteins (PAGs) have been previously associated with EM/FM. The aim of the present study was to test the ability of a semi- quantitative enzyme-linked immunosorbent assay for the detection of PAGs(PAG-ELISA) to either diagnose (EM/FM has already occurred) or to predict (EM/FM will occur) EM/FM. Three experiments were carried out as follows: (1) PAG-ELISA validation, (2) aretro-spective verification of 141 PAG results in cases with suspected EM/FM and the identification of cut-off values for EM/FM, and (3) a field experiment, testing the proposed PAG-ELISA cut-off values defined in Experiment 2 under farm conditions by comparing transrectal ultrasound (TRU) results with PAG-ELISA results. The PAG-ELISA validation (Experiment 1) demonstrated that anoptical density (OD) range of 0.4–2.8 reflected a linear relation to the PAG concentrations. The results from Experiment 2 indicated that more cows (85.7%) with PAG-OD values ranging from 0.40–0.79 showed EM/FM compared to cows (21.7%) with PAG-OD values ranging from 1.2 to 1.5. Acut-off valueof 2.2 (sensitivity [SEN]=87.6%; specificity [SPE]=72.8%) was determined using ROC-curve analysis for prediction of EM/FM and a cut-off value of 1.2 for diagnosis of EM/FM (SEN=50%; SPE=94%). However, in the field experiment, EM/FM was best diagnosed using a cut-off value of 1.5 (SEN=26.7% and SPE=96.5%). Hence, the high SEN detected in Experiment 2 could not be confirmed in the field experiment indicating that a singular PAG value is not a convenient tool to diagnose EM/FM. Though, the combined use of PAG-ELISA and TRU showed remarkable results (SEN=84.2%; SPE=98.1%) by revealing a technique for diagnosis of previously occurred EM/FM and for prediction of subsequent occurring EM/FM. [less ▲]

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See detailIdentification of equid herpesvirus-5 in respiratory liquids: A retrospective study of 785 samples taken in 2006-2007
Fortier, Guillaume; Pronost, Stéphane; Miszczak, Fabien et al

in Veterinary Journal (2009), 182

During a case control study undertaken in 2006-2007, a screening and consensus polymerase chain reaction (PCR) was performed to evaluate the potential role of equid herpesviruses (EHV) in several ... [more ▼]

During a case control study undertaken in 2006-2007, a screening and consensus polymerase chain reaction (PCR) was performed to evaluate the potential role of equid herpesviruses (EHV) in several occurrences of respiratory disorders in 661 horses. Of 785 bronchoalveolar or tracheal lavage fluid samples submitted for analysis, 20 were positive for EHV-5 DNA by sequential analysis of the consensus PCR product. Nineteen of those samples were confirmed using a specific EHV-5 PCR. No particular changes in cytological profile could be associated with the detection of EHV-5 in contrast to suggestions in previous reports of natural or experimental respiratory viral infections in horses or ponies. This is the first description of EHV-5 isolation in equine respiratory fluids in Europe, but further investigations are needed to determine the potential pathogenic role of this gammaherpesvirus in the horse. [less ▲]

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See detailThe identification of experimental microscopic hafting traces on lithic tools
Rots, Veerle ULg

in Le Sécretariat du Congrès (Ed.) Sessions Générales et Posters / General Sessions and Posters. Acts of the XIVth UISPP Congress University of Liège, Belgium, 2 – 8 September 2001 (2003)

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See detailIdentification of factors important for the success of suicide gene therapy after a comparative study of Varicella zoster and Herpes simplex viral thymidine kinases efficacy on breast cancer cells
Grignet-Debrus, Christine ULg; Noël, Agnès ULg; Foidart, Jean-Michel ULg et al

in Cellular and Molecular Biology (2005), 51(1), 37-48

One of the most frequently studied therapeutic strategies in the field of suicide gene therapy is based on the expression by tumor cells of the Herpes simplex virus thymidine kinase gene (HSVtk) followed ... [more ▼]

One of the most frequently studied therapeutic strategies in the field of suicide gene therapy is based on the expression by tumor cells of the Herpes simplex virus thymidine kinase gene (HSVtk) followed by a ganciclovir (GCV) treatment. In order to investigate the potential of other enzyme/prodrug strategies, we studied in vitro and in vivo the ability of the Varicella zoster virus thymidine kinase gene (VZVtk) to act as a suicide gene and to kill non-transduced bystander cells, and compared this activity to that of its HSV counterpart. Four different antiviral compounds were tested as prodrugs. Our comparative study demonstrates the superiority of the HSVtk/GCV system among the different combinations tested and underlines the importance of both the tumor cell type and the prodrug in the success of a prodrug/suicide gene strategy. [less ▲]

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See detailIdentification of factors that maintain mammalian outer hair cells in adult organ of Corti explants
Malgrange, Brigitte ULg; Rigo, Jean-Michel; Coucke, Paul et al

in Hearing Research (2002), 170(1-2), 48-58

Both outer hair cells (OHCs) and inner hair cells (IHCs) survive and mature in 3 days old rat organ of Corti explants cultured for I month in a minimal essential medium. In contrast. under the same ... [more ▼]

Both outer hair cells (OHCs) and inner hair cells (IHCs) survive and mature in 3 days old rat organ of Corti explants cultured for I month in a minimal essential medium. In contrast. under the same culture conditions, only IHCs survive in explants from adult guinea pig organ of Corti while many of the OHCs are lost within the first 48 It. Hair cell Count,, show OHCs loss to be greater in the lower portion (i.e. middle turn) of the cochlea than Lit the apex. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) indicates that there is DNA damage in adult OHCs, within 8 h of explantation. Treatment of the adult organ of Corti explants with either actinomycin D (10(-7) M) or cycloheximide (10(-6) M) prevents most OHC losses . According to these results apoptosis may be the mechanism of OHC loss in adult organ of Corti explants, Stable membrane potentials recorded from the OHCs in both uncultured and actinomycin D-treated organ of Corti explants cultured for 72 h demonstrate the functional integrity of these hair cells. OHC losses in the adult guinea pig, organ of Corti cultures can also be prevented by treatment with several of the growth factors tested. i.e. acidic fibroblast growth factor (aFGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), transforming growth factor-beta1 (TGF-beta1). and glial cell-derived neurotrophic factor (GDNF). The results of this study suggest that growth factor therapy may be applicable to the treatment of some hearing disorders. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailIdentification of feline panleukopenia virus proteins expressed in Purkinje cell nuclei of cats with cerebellar hypoplasia
Poncelet, Luc; Héraud, Céline; Springinsfeld, Marie et al

in The Veterinary Journal (2013), 196(3), 381-387

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See detailIdentification of Fragmentation Channels of Dinucleotides Using Deuterium Labeling.
Balbeur, D.; Dehareng, Dominique ULg; De Pauw, Edwin ULg

in Journal of the American Society for Mass Spectrometry (2010), 21(1), 23-33

The fragmentation of the totally deuterated dinucleotide dAT(-) in labile positions (heteroatom-bound hydrogens) was compared for different MS/MS methods: CID, IRMPD, and EID. These experiments allowed us ... [more ▼]

The fragmentation of the totally deuterated dinucleotide dAT(-) in labile positions (heteroatom-bound hydrogens) was compared for different MS/MS methods: CID, IRMPD, and EID. These experiments allowed us to affirm the coexistence of several fragmentation channels. They can be classified according to the involvement of nonlabile or labile protons in the fragmentation process. Moreover, double resonance experiments were performed in IRMPD and EID. They demonstrated the existence of consecutive fragmentation processes. The probability with which each channel is taken depends on the fragmentation technique used, i.e., the energy and the time scale of the method. The fragmentation channels that involve labile protons requiring peculiar three-dimensional structures are entropically unfavorable and enthalpically favorable. They are more observed in IRMPD and EID. The involvement of labile and, therefore, exchangeable protons in the fragmentation mechanism casts doubt on the use of tandem mass spectrometry to localize incorporated deuteriums in oligonucleotides. [less ▲]

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See detailIdentification of functional elements and regulatory circuits by Drosophila modENCODE.
Roy, Sushmita; Ernst, Jason; Kharchenko, Peter V. et al

in Science (2010), 330(6012), 1787-97

To gain insight into how genomic information is translated into cellular and developmental programs, the Drosophila model organism Encyclopedia of DNA Elements (modENCODE) project is comprehensively ... [more ▼]

To gain insight into how genomic information is translated into cellular and developmental programs, the Drosophila model organism Encyclopedia of DNA Elements (modENCODE) project is comprehensively mapping transcripts, histone modifications, chromosomal proteins, transcription factors, replication proteins and intermediates, and nucleosome properties across a developmental time course and in multiple cell lines. We have generated more than 700 data sets and discovered protein-coding, noncoding, RNA regulatory, replication, and chromatin elements, more than tripling the annotated portion of the Drosophila genome. Correlated activity patterns of these elements reveal a functional regulatory network, which predicts putative new functions for genes, reveals stage- and tissue-specific regulators, and enables gene-expression prediction. Our results provide a foundation for directed experimental and computational studies in Drosophila and related species and also a model for systematic data integration toward comprehensive genomic and functional annotation. [less ▲]

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See detailIdentification Of Functional-Sites On Bovine Leukemia-Virus Envelope Glycoproteins Using Structural And Immunological Data
Callebaut, I.; Portetelle, Daniel ULg; Burny, A. et al

in European Journal of Biochemistry (1994), 222(2),

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See detailIdentification of genes involved in the response of banana to crown rot disease
Lassois, Ludivine ULg; Frettinger, Patrick; de Lapeyre de Bellaire, Luc et al

in Molecular Plant-Microbe Interactions [=MPMI] (2011), 24(1), 143-153

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See detailIdentification of genes that function in the biogenesis and localization of small nucleolar RNAs in Saccharomyces cerevisiae.
Qiu, Hui; Eifert, Julia; Wacheul, Ludivine et al

in Molecular & Cellular Biology (2008), 28(11), 3686-99

Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs transiently ... [more ▼]

Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs transiently localize to the Cajal body (in plant and animal cells) or the homologous nucleolar body (in budding yeast) for maturation and assembly into snoRNPs prior to accumulation in their primary functional site, the nucleolus. However, little is known about the trans-acting factors important for the intranuclear trafficking and nucleolar localization of snoRNAs. Here, we describe a large-scale genetic screen to identify proteins important for snoRNA transport in Saccharomyces cerevisiae. We performed fluorescence in situ hybridization analysis to visualize U3 snoRNA localization in a collection of temperature-sensitive yeast mutants. We have identified Nop4, Prp21, Tao3, Sec14, and Htl1 as proteins important for the proper localization of U3 snoRNA. Mutations in genes encoding these proteins lead to specific defects in the targeting or retention of the snoRNA to either the nucleolar body or the nucleolus. Additional characterization of the mutants revealed impairment in specific steps of U3 snoRNA processing, demonstrating that snoRNA maturation and trafficking are linked processes. [less ▲]

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See detailIDENTIFICATION OF GENETIC SUSCEPTIBILITY FACTORS FOR FIBROMYALGIA
Docampo Martínez, Elisa ULg

Doctoral thesis (2013)

Fibromyalgia (FM) is a highly disabling syndrome defined by a low pain threshold and a permanent state of pain. Widespread pain is accompanied by a constellation of symptoms such as fatigue, sleep ... [more ▼]

Fibromyalgia (FM) is a highly disabling syndrome defined by a low pain threshold and a permanent state of pain. Widespread pain is accompanied by a constellation of symptoms such as fatigue, sleep disturbances and cognitive impairment, among others. The mechanisms explaining this chronic pain remain unclear. Nowadays, the most established/ plausible hypothesis underlying FM ethiopathogenesis is the existence of a dysfunction in pain processing, as supported by alterations in neuroimaging and neurotransmitters levels. The etiology of FM involves the interaction of environmental and genetic susceptibility factors. The genetic contribution to FM has been proven by the presence of a higher concordance of monozygotic than dizygotic twins as well as family aggregation. However, the individual genetic and environmental factors involved have not been identified. The aim of this thesis was to elucidate genetic susceptibility factors for fibromyalgia. We assessed this objective through three main approaches: the identification of FM clinically homogeneous subgroups with a two step cluster analyses, a genome-wide association study in order to evaluate the possible contribution of single nucleotide polymorphisms with Illumina 1 million duo array, and array comparative genomic hybridization experiments to identify regions varying in copy number that could be involved in FM susceptibility,using Agilent 2X400K platform. 48 variables were evaluated in 1,446 Spanish FM cases fulfilling 1990 ACR FM criteria. A partitioning analysis was performed to find groups of variables similar to each other. Variables clustered into three independent dimensions: “symptomatology”, “comorbidities” and “clinical scales”. Only the two first dimensions were considered for the construction of FM subgroups, classifying FM samples into three subgroups: low symptomatology and comorbidities (Cluster 1), high symptomatology and comorbidities (Cluster 2), and high symptomatology but low comorbidities (Cluster 3). These subgroups showed differences in measures of disease severity and were further implemented in genetic analysis. Genome-wide association study was performed in 300 FM cases and 203 controls. No SNP reached GWAS association threshold, but 21 of the most associated SNPs were chosen for replication in over 900 cases and 900 pain free-controls. Four of the strongest associated SNPs selected for replication showed a nominal association in the joint analysis. In particular, rs11127292 (MYT1L) was found to be associated to FM with low comorbidities. Array comparative genomic hybridization detected 5 differentially hybridized regions. They were followed up and one of these regions was validated though a multiplex PCR experiment. An intronic deletion in NRXN3 showed to be associated to female cases of FM and in particular those with low levels of comorbidities. Replication analysis showed a stronger association when considering only female cases and controls and low comorbidities. This enhance the importance of gender in FM etiopathogenesis and could be pointing to the existence of a different genetic background for FM in males and females highlights the importance of identifying FM homogeneous subgroups for the detection of FM genetic susceptibility factors. If the proposed FM candidate genes are further validated in replication studies, this would constitute a change in the FM ethiologycal concept, as several of these candidates are known neuropsychiatric disease associated genes (autism, addiction, mental disability). This would highlight a novel neurocognitive involvement in this disorder, currently considered musculoskeletal and affective. [less ▲]

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See detailIdentification of groundwater quality trends in a chalk aquifer threatened by intensive agriculture in Belgium
Batlle-Aguilar, Jordi; Orban, Philippe ULg; Dassargues, Alain ULg et al

in Hydrogeology Journal (2007), 15(8), 1615-1627

The European Union (EU) has adopted directives requiring that Member States take measures to reach a "good" chemical status of water resources by the year 2015 (Water Framework Directive: WFD). In order ... [more ▼]

The European Union (EU) has adopted directives requiring that Member States take measures to reach a "good" chemical status of water resources by the year 2015 (Water Framework Directive: WFD). In order to achieve the environmental objectives for groundwater, the identification and reversal of significant upward trends in pollutant concentrations are required. A very detailed dataset available for the Hesbaye chalk aquifer in Belgium is used to evaluate tools and to propose efficient methodologies for identifying and quantifying nitrate trends in groundwater. Results indicate that the parametric linear regression and the non-parametric Mann-Kendall tests are robust; however, the latter test seems more adequate as it does not require verification of the normality of the dataset and it provides calculated nitrate trends very comparable to those obtained using linear regression. From a hydrogeological point of view, results highlight a general upward trend in the whole groundwater basin. The extrapolation of the trend analysis results indicates that measures have to be taken urgently in order to avoid further major degradation of groundwater quality within the next 10-70 years. However, a good groundwater quality status cannot be expected in the Hesbaye aquifer for the 2015 EU WFD deadline. [less ▲]

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See detailIdentification of groundwater quality trends in a chalky aquifer threatened by intensive agriculture
Batlle-Aguilar, Jordi; Orban, Philippe ULg; Dassargues, Alain ULg et al

in Stauffer, Fr. (Ed.) Quantitative Geology from Multiple Sources: S10 Use of multiple sources in conditioning/calibrating groundwater flow and transport models (2006)

Diffuse groundwater contamination related to agricultural practices is a worldwide environmental problem, particularly the continuous increase in nitrate concentrations. As a response to this threat, the ... [more ▼]

Diffuse groundwater contamination related to agricultural practices is a worldwide environmental problem, particularly the continuous increase in nitrate concentrations. As a response to this threat, the European Union has adopted directives requiring that Member States take measures to reduce agricultural nitrate sources and stating that a “good” status of groundwater is required for all EU members. In order to achieve the environmental objectives for groundwater, the identification and reversal of any significant upward trend in the pollutant concentrations are required. Conclusions are drawn about the evolution of groundwater contamination by nitrates in the following decades with respect to the EU Water directive prescriptions. Measures have to be urgently taken in order to avoid major degradation of groundwater in 10 to 70 years. However, a good groundwater quality status cannot be expected for the 2015 EU Water Framework Directive (WFD). [less ▲]

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See detailIdentification of growth-hormone binding proteins (GBBPs) in cattle
Devolder, Anne; Renaville, Robert ULg; Massart, Serge et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailIdentification of hard ticks (Acari: Ixodidae) and seroprevalence to Theileria parva in cattle raised in North Kivu Province, Democratic Republic of Congo.
Kalume, Moise Kasereka; Saegerman, Claude ULg; Mbahikyavolo, Daniel Kambale et al

in Parasitology research (2013), 112(2), 789-97

This study aimed to identify tick species and to determine their relationship with the Theileria parva seroprevalence in cattle raised under an extensive farming system in North Kivu Province, Democratic ... [more ▼]

This study aimed to identify tick species and to determine their relationship with the Theileria parva seroprevalence in cattle raised under an extensive farming system in North Kivu Province, Democratic Republic of Congo in two agro-ecological zones namely medium (1,000-1,850 m) and high (>1,850 m) altitude. Among the 3,215 ticks collected on 482 animals, from February to April 2009, Rhipicephalus appendiculatus (64.26 %), the main vector of T. parva, was the most abundant species followed by Rhipicephalus decoloratus (35.49 %) and Amblyomma variegatum (0.25 %). The mean burden of R. appendiculatus tick per infested animal appeared significantly higher at medium (6.5 +/- 0.22 ticks) than at high (0.07 +/- 0.3 ticks) altitude (P < 0.05). However, an indirect fluorescent antibody test carried out on 450 blood samples revealed a global T. parva seroprevalence of 43 % (95 % CI: 38-47) which was not significantly (P > 0.05) different between medium (48.4 %; 95 % CI: 38-49) and high (41.9 %; 95 % CI: 35-49) altitude. These relatively low seroprevalences suggest that there is a state of endemicity to T. parva infection in the study area. The presence of the tick vector on animals was associated with an increased risk of being seropositive to T. parva infection (odds ratio = 2.04; 95 % CI: 1.8-2.3; P < 0.001). The results suggest the need for a longitudinal study to investigate the seasonal dynamics of tick species and T. parva infection. The rate of tick infection should also be evaluated in order to determine the intensity of T. parva transmission to cattle. [less ▲]

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See detailIdentification of hardening behavior using inverse modeling and image correlation
Lecompte, D.; Cooreman, S.; Sol, H. et al

in Proceedings of the SEM annual conference and exposition 2006 (2006)

The basic principle of an inverse modeling procedure as it is used for parameter identification, is the generation of a complex and heterogeneous deformation field that contains as much information as ... [more ▼]

The basic principle of an inverse modeling procedure as it is used for parameter identification, is the generation of a complex and heterogeneous deformation field that contains as much information as possible about the parameters to be identified. One way of obtaining such a non-homogeneous deformation is by making the geometry of the specimen less regular. Another possibility is to make the loading conditions more complex. In this paper both options are actually combined by using a biaxial tensile test on a cruciform specimen in order to identify the parameters of a Swift isotropic hardening law. The yield criterion is modeled by the isotropic Von Mises criterion. The optimization technique used is a constrained gradient based Newton-type routine, which means that in every iteration step, a sensitivity calculation has to be performed in order to indicate the direction in which the parameters are to be optimized. The functional to be minimized is a least-squares expression of the discrepancy between the measured and the simulated strain fields at a certain load. The numerical routines as well as the identification results, based on simulated strain fields, are discussed. [less ▲]

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