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See detailLocalization and quantification of damage in beam-like structures using sensitivities of principal component analysis results
Nguyen, Viet Ha ULg; Golinval, Jean-Claude ULg

in Mechanical Systems & Signal Processing (2010), 24(6), 1831-1843

Principal component analysis (PCA) is known as an efficient method for dynamic system identification and diagnosis. This paper addresses a damage diagnosis method based on sensitivities of PCA in the ... [more ▼]

Principal component analysis (PCA) is known as an efficient method for dynamic system identification and diagnosis. This paper addresses a damage diagnosis method based on sensitivities of PCA in the frequency domain for linear-form structures. The aim is not only to detect the presence of damage, but also to localize and to evaluate it. The Frequency response functions measured at different locations on the beam are considered as data for the PCA process. Sensitivities of principal components obtained from PCA to beam parameters are computed and inspected according to the location of sensors; their variation from the healthy state to the damaged state indicates damage locations. The damage can be evaluated next providing that a structural model is available; this evaluation is based on a model updating procedure. It is worth noting that the diagnosis process does not require a modal identification achievement. Both numerical and experimental examples are used for better illustration. [less ▲]

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See detailLocalization and transmembrane topology of a new member of the mitochondrial carrier family, the yeast RIM 2 gene product
Elmoualij, Benaïssa ULg; Duyckaerts, Claire ULg; Lamotte-Brasseur, J. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1995)

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See detailLocalization by immunofluorescent microscopy of several collagen types and of a basement membrane proteoglycan in rat glomerular epithelial and mesangial cell cultures.
Foidart, J. B.; Foidart, Jean-Michel ULg; Hassell, J. et al

in Renal Physiology (1983), 6(4), 163-70

Confluent cultures of rat glomerular epithelial and mesangial cells were studied by immunofluorescent microscopy, using affinity-purified antibodies directed against collagen of type I-V or an antiserum ... [more ▼]

Confluent cultures of rat glomerular epithelial and mesangial cells were studied by immunofluorescent microscopy, using affinity-purified antibodies directed against collagen of type I-V or an antiserum directed against a basement membrane (BM) proteoglycan. The epithelial cells were stained by antibodies directed against type I, IV and V collagen, whereas the mesangial cells were stained by all the antibodies directed against the different collagenous antigens tested. Therefore, only mesangial cells contained antigenic determinants of type III collagen. On the contrary, both cell types possessed BM proteoglycan antigens. The data suggest that rat glomerular epithelial and mesangial cells may be implicated in the biosynthesis of different components of normal (and pathological) glomerular BM. [less ▲]

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See detailLocalization of DNA in nucleoli with and without fibrillar centers
Thiry, Marc ULg

Conference (1998)

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See detailLocalization of DNA within Ehrlich tumour cell nucleoli by immunoelectron microscopy.
Thiry, Marc ULg; Scheer, U.; Goessens, Guy ULg

in Biology of the Cell (1988), 63(1), 27-34

The distribution of DNA in Ehrlich tumour cell nucleoli was investigated by means of an immunocytochemical approach involving a monoclonal antibody directed against double- and single-stranded DNA ... [more ▼]

The distribution of DNA in Ehrlich tumour cell nucleoli was investigated by means of an immunocytochemical approach involving a monoclonal antibody directed against double- and single-stranded DNA. Immunolabelling was performed either before or after the embedding process. The postembedding labelling method allows better ultrastructural preservation than the preembedding labelling method. In particular, the various nucleolar components are well preserved and identifiable. In the nucleolus, labelling is particularly concentrated over the perinucleolar chromatin and over its intranucleolar invaginations, which penetrate the nucleolar body and often terminate at the fibrillar centres. In addition, aggregates of gold particles are found in the fibrillar centres, preferentially towards the peripheral regions. By contrast, the dense fibrillar component is completely devoid of labelling. The results seem to indicate that DNA containing the rDNA genes is located in the fibrillar centres, with a preference for the peripheral regions. This finding suggests that transcription of the rDNA genes should occur within the confines of the fibrillar centre, probably close to the boundary region of the surrounding dense fibrillar component. The results are discussed in the light of present knowledge of the functional organization of the nucleolus. [less ▲]

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See detailLocalization of DNase I sensitive sequences in specific regions of nuclear architecture
Thiry, Marc ULg

in Archives Internationales de Physiologie et de Biochimie (1990), 98

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See detailLOCALIZATION OF ENERGY IN A PERFECTLY SYMMETRIC BLADED DISK ASSEMBLY DUE TO NONLINEARITIES
Georgiades, Fotios; Peeters, Maxime ULg; Kerschen, Gaëtan ULg et al

in ASME Internaional Mechanical Engineering Congress and Exposition, Seattle, 2007 (2007, November)

Although a bladed disk is typically designed to have identical blades, manufacturing tolerances, wear, and other causes may cause random deviations among the blades. The blade-to-blade discrepancies ... [more ▼]

Although a bladed disk is typically designed to have identical blades, manufacturing tolerances, wear, and other causes may cause random deviations among the blades. The blade-to-blade discrepancies, denoted as mistuning, lead to vibratory responses mostly concentrated in small regions of the bladed-disk assembly, according to a phenomenon called localization. The resulting spatial confinement of the vibration energy causes the responses of some blades to become dangerously high and increases the amplitude of the bladed-disk assembly's overall response. The attendant increase in stresses can lead to premature high cycle fatigue (HCF) of the blades. In this study we investigate whether vibration localization in a perfectly symmetric bladed disk assembly may occur in the presence of nonlinearity. To this end, the nonlinear normal modes (NNMs) of a simplified model of a bladed disk assembly are computed. The NNMs are then carefully examined to highlight possible vibration localization phenomena. [less ▲]

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See detailLocalization of gonadotropins in pituitaries of various animals especies and in humans
Bastings, E.; Beckers, Albert ULg; Reznik, M. et al

in de la Cruz, L. F.; Garcia Luna, M. T. (Eds.) Recent advances in growth and reproduction (1990)

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See detailLocalization of human transcription factor TEF-4 and TEF-5 (TEAD2, TEAD3) genes to chromosomes 19q13.3 and 6p21.2 using fluorescence in situ hybridization and radiation hybrid analysis
Jacquemin, Patrick; Depetris, Danielle; Mattei, Marie-Geneviève et al

in Genomics (1999), 55(1), 127-9

Embryonic TEA domain-containing factor (ETF) belongs to the family of proteins structurally related to transcriptional enhancer factor-1 (TEF-1) and is implicated in neural development. Isolation and ... [more ▼]

Embryonic TEA domain-containing factor (ETF) belongs to the family of proteins structurally related to transcriptional enhancer factor-1 (TEF-1) and is implicated in neural development. Isolation and characterization of the cosmid clones encoding the mouse ETF gene (Etdf) revealed thatEtdfspans approximately 17.9 kb and consists of 12 exons. The exon–intron structure ofEtdfclosely resembles that of theDrosophila scallopedgene, indicating that these genes may have evolved from a common ancestor. The multiple transcription initiation sites revealed by S1 protection and primer extension analyses are consistent with the absence of the canonical TATA and CAAT boxes in the 5′-flanking region, which contains many potential regulatory sequences, such as the E-box, N-box, Sp1 element, GATA-1 element, TAATGARAT element, and B2 short interspersed element (SINE) as well as several direct and inverted repeat sequences. TheEtdflocus was assigned to the proximal region of mouse chromosome 7 using fluorescencein situhybridization and linkage mapping analyses. These results provide the molecular basis for studying the regulation,in vivofunction, and evolution ofEtdf. *1 The approved gene symbol for embryonic TEA domain-containing factor (ETF) by MGD isEtdf.The nucleotide sequences reported in this paper have been deposited with the GenBank/EMBL/DDBJ Data Libraries under Accession Nos. D83586–D83596. Mapping data from this article have been deposited in the Mouse Genome Database under Accession Nos. MGD-CREX-618 for genetic mapping and MGD-INEX-21 forin situhybridization, respectively. [less ▲]

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See detailLocalization of interleukin 6 mRNA in human tonsils by in situ hybridization.
Bosseloir, A.; Hooghe-Peters, E. L.; Heinen, Ernst ULg et al

in European Journal of Immunology (1989), 19(12), 2379-81

We have investigated which areas produce interleukin 6 (IL 6) in human tonsils. This growth factor is required for the terminal differentiation of B lymphocytes into plasmocytes. Using 35S-labeled IL 6 ... [more ▼]

We have investigated which areas produce interleukin 6 (IL 6) in human tonsils. This growth factor is required for the terminal differentiation of B lymphocytes into plasmocytes. Using 35S-labeled IL 6 cDNA we demonstrated IL 6 gene expression over various areas of the tonsils, with consistent exception of the follicles, by in situ hybridization. It is, therefore, proposed that B cells are stimulated during their migration out of the follicles. [less ▲]

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See detailLocalization of Laminin, Fibronectin, E-Cadherin, and Integrins in Endometrium and Endometriosis
Beliard, Aude ULg; Donnez, J.; Nisolle, Michelle ULg et al

in Fertility and Sterility (1997), 67(2), 266-72

OBJECTIVE: To compare the localization of adhesion proteins (laminin and fibronectin) and their receptors of the integrin family in endometriosis and endometrium. DESIGN: An immunohistochemical study ... [more ▼]

OBJECTIVE: To compare the localization of adhesion proteins (laminin and fibronectin) and their receptors of the integrin family in endometriosis and endometrium. DESIGN: An immunohistochemical study. SETTING: University Hospital, Department of Gynecology and Department of Cell Biology. PATIENT(s): Eighteen endometriosis patients undergoing laparoscopy for pain or infertility and nine control patients undergoing laparoscopy for sterilization or hysterectomy. MAIN OUTCOME MEASURE(s): The expression of adhesion glycoproteins (laminin and fibronectin), their receptors alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, and alpha 6 beta 1, and E-cadherin was determined by immunohistochemistry on frozen sections. RESULT(s): The distribution of both adhesive glycoproteins, laminin and fibronectin, and their receptors was identical in endometriosis and endometrium. Fibronectin receptors (alpha 4 beta 1, alpha 5 beta 1) displayed distinct expression patterns in endometrium and endometriosis. No endometrial glands showed positive staining for the alpha 5 chain, whereas this integrin subunit was detected in almost all endometriotic lesions. The integrin alpha 4 chain was present in all endometriotic glands but was absent from endometrial glands in the proliferative phase of the cycle. CONCLUSION(s): No difference in cell adhesion molecule localization nor receptors was observed between endometriotic and endometrial samples, except for fibronectin receptors. Their expression persisted around endometriotic glands but not in endometrium. These results suggest that fibronectin receptors could play a role in the persistence of endometriotic lesions, despite menstruation in corresponding eutopic endometrium. [less ▲]

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See detailLocalization of Nadph-Protochlorophyllide Reductase in Plastids of Barley at Different Greening Stages
Barthelemy, X.; Bouvier, G.; Radunz, A. et al

in Photosynthesis Research (2000), 64(1), 63-76

The localization of protochorophyllide (Pchlide) and of NADPH-protochlorophyllide oxidoreductase (POR, EC 1.6.99.1) within (etio)chloroplasts has been investigated at selected stages of greening of barley ... [more ▼]

The localization of protochorophyllide (Pchlide) and of NADPH-protochlorophyllide oxidoreductase (POR, EC 1.6.99.1) within (etio)chloroplasts has been investigated at selected stages of greening of barley seedlings. Pchlide pigment and POR protein contents were evaluated in different plastid membrane fractions by fluorescence spectroscopy and immunoblot analysis using a monospecific polyclonal antibody raised against the purified enzyme. Fluorescence analysis showed the presence of Pchlide in both the envelope and thylakoid membranes. During greening, the Pchlide content, expressed on a total protein basis, decreased in thylakoid membranes, whereas it increased in the envelope membranes. POR proteins were detected mainly in thylakoid membranes at early greening stages. In contrast, the weak amount of POR proteins was associated more specifically with envelope membranes of mature chloroplasts. Whatever the greening stage, thylakoid-bound Pchlide and POR proteins were more abundant in the thylakoid regions which remained unsolubilized after mild Triton treatment used as standard procedure to prepare PS II particles. This suggests the preferential association of Pchlide and POR to the appressed regions of thylakoids. [less ▲]

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See detailLocalization of Nopp140 within mammalian cells during interphase and mitosis
Thiry, Marc ULg; Cheutin, Thierry; Lamaye, Françoise et al

Poster (2009)

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See detailLocalization of Nopp140 within mammalian cells during interphase and mitosis.
Thiry, Marc ULg; Cheutin, Thierry; Lamaye, Françoise ULg et al

in Histochemistry & Cell Biology (2009), 132(2), 129-40

We investigated distribution of the nucleolar phosphoprotein Nopp140 within mammalian cells, using immunofluorescence confocal microscopy and immunoelectron microscopy. During interphase, three ... [more ▼]

We investigated distribution of the nucleolar phosphoprotein Nopp140 within mammalian cells, using immunofluorescence confocal microscopy and immunoelectron microscopy. During interphase, three-dimensional image reconstructions of confocal sections revealed that nucleolar labelling appeared as several tiny spheres organized in necklaces. Moreover, after an immunogold labelling procedure, gold particles were detected not only over the dense fibrillar component but also over the fibrillar centres of nucleoli in untreated and actinomycin D-treated cells. Labelling was also consistently present in Cajal bodies. After pulse-chase experiments with BrUTP, colocalization was more prominent after a 10- to 15-min chase than after a 5-min chase. During mitosis, confocal analysis indicated that Nopp140 organization was lost. The protein dispersed between and around the chromosomes in prophase. From prometaphase to telophase, it was also detected in numerous cytoplasmic nucleolus-derived foci. During telophase, it reappeared in the reforming nucleoli of daughter nuclei. This strongly suggests that Nopp140 could be a component implicated in the early steps of pre-rRNA processing. [less ▲]

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See detailLocalization of nucleic acids in hepatocyte nucleoli of rats upon D-galactosamine-induced block of transcription.
Mikhaylova, V. T.; Thiry, Marc ULg; Stephanova, E. et al

in Experimental Cell Research (1996), 225(2), 389-98

The precise localization of DNA and RNA within rat hepatocyte nucleoli during the process of D-galactosamine-induced nucleolar segregation has been studied by using sensitive methods for their detection ... [more ▼]

The precise localization of DNA and RNA within rat hepatocyte nucleoli during the process of D-galactosamine-induced nucleolar segregation has been studied by using sensitive methods for their detection: osmium-ammine staining and terminal deoxynucleotidyl transferase reaction for DNA, and immunoelectron microscopy with anti-RNA antibodies, RNase-gold, and autoradiography with tritiated orotic acid for RNA. The blocking of transcription was followed by the disappearance of intranucleolar condensed chromatin. Agglomerates of thin extended DNA filaments were found to change their location to the nucleolar periphery and to coalesce with each other. At the last stage of nucleolar segregation they were concentrated at the pole of the nucleolar fibrillar remnant while the rest of the nucleolus did not contain any DNA. No DNA was found in the dense fibrillar component of both intact and treated hepatocyte nucleoli. During the process of nucleolar segregation the bulk of the nucleolar RNA was found within the so-called spherical bodies. This RNA appeared to be synthesized shortly before or even after drug administration. The results obtained are in agreement with the hypothesis that the fibrillar centers are the site of nucleolar transcription. They also show that uncompleted molecules of pre-rRNA whose synthesis has been blocked are segregated from the rest of nucleolar RNA species into the spherical bodies. [less ▲]

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See detailLocalization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level.
Thiry, Marc ULg; Scheer, U.; Goessens, Guy ULg

in Electron Microscopy Reviews (1991), 4(1), 85-110

Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well ... [more ▼]

Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure. Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA topoisomerase I are located together. Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component. [less ▲]

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See detailLocalization of oestrogen receptors in the sensory and motor areas of the spinal cord in Japanese quail (Coturnix japonica)
Evrard, H. C.; Balthazart, Jacques ULg

in Journal of Neuroendocrinology (2002), 14(11), 894-903

In Japanese quail, the presence of aromatase (oestrogen synthase) in the dorsal horn of the spinal cord suggests that spinal sensory processes might be controlled by local actions of oestrogens. This is ... [more ▼]

In Japanese quail, the presence of aromatase (oestrogen synthase) in the dorsal horn of the spinal cord suggests that spinal sensory processes might be controlled by local actions of oestrogens. This is supported by the presence of oestrogen receptors and aromatase in the dorsal horn of the spinal cord in rats, and by the alteration of sensitivity by oestrogens in various mammalian species and also in canaries. We investigated whether oestrogens that are locally produced in the quail spinal cord can bind to specific receptors in the vicinity of their site of synthesis. We demonstrate the presence of numerous oestrogen receptor alpha-immunoreactive (ERalpha-ir) cell nuclei, predominantly in laminae II and, to a lesser extent, I and III of the dorsal horn of the spinal cord (i.e. in the area where aromatase was previously identified). ERalpha-ir cells were also seen in various parts of the intermediate zone (laminae V-VII). This presence of ERalpha-ir cells in the dorsal horn and intermediate zone fits in well with the distribution of ERalpha-ir cells in homologous areas in mammals, including rats. Only a few labelled cells were found in the ventral horn in the cervical, brachial, thoracic and first lumbar segments, but a conspicuous dense group of large ERalpha-ir cells was identified in lamina IX of the ventral horn in synsacral segments 8-10, which contain the motoneurones innervating the muscles of the cloacal gland. The presence of ERalpha-ir cells in lamina IX of these synsacral segments in quail contrasts with the finding that motoneurones innervating penile muscles in rats contain androgen, but not oestrogen receptors, and are influenced by androgens rather than by oestrogens. Together, these data suggest that spinal actions of oestrogens may modulate the sensory and motor systems that participate in reproduction, as well as other nonreproductive functions in quail. [less ▲]

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See detailLocalization of superconductivity in superconductor–electromagnet hybrids
Ataklti, G.W.; Aladyshkin, A.Yu.; Gillijns, W. et al

in Superconductor Science and Technology (2012), 25

We investigate the nucleation of superconductivity in a superconducting Al strip under the influence of the magnetic field generated by a current-carrying Nb wire, perpendicularly oriented and located ... [more ▼]

We investigate the nucleation of superconductivity in a superconducting Al strip under the influence of the magnetic field generated by a current-carrying Nb wire, perpendicularly oriented and located underneath the strip. The inhomogeneous magnetic field, induced by the Nb wire, produces a spatial modulation of the critical temperature Tc, leading to a controllable localization of the superconducting order parameter (OP) wavefunction. We demonstrate that close to the phase boundary Tc(Bext) the localized OP solution can be displaced reversibly by either applying an external perpendicular magnetic field Bext or by changing the amplitude of the inhomogeneous field. [less ▲]

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