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See detailHydrophobin Fusion of an Influenza Virus Hemagglutinin Allows High Transient Expression in Nicotiana benthamiana, Easy Purification and Immune Response with Neutralizing Activity
Jacquet, Nicolas; Navarre, Catherine; Desmecht, Daniel ULg et al

in PLoS ONE (2014), 9(12), 115944

The expression of recombinant hemagglutinin in plants is a promising alternative to the current egg-based production system for the influenza vaccines. Protein-stabilizing fusion partners have been ... [more ▼]

The expression of recombinant hemagglutinin in plants is a promising alternative to the current egg-based production system for the influenza vaccines. Protein-stabilizing fusion partners have been developed to overcome the low production yields and the high downstream process costs associated with the plant expression system. In this context, we tested the fusion of hydrophobin I to the hemagglutinin ectodomain of the influenza A (H1N1)pdm09 virus controlled by the hybrid En2PMA4 transcriptional promoter to rapidly produce high levels of recombinant antigen by transient expression in agro-infiltrated Nicotiana benthamiana leaves. The fusion increased the expression level by a factor of ∼2.5 compared to the unfused protein allowing a high accumulation level of 8.6% of the total soluble proteins. Hemagglutinin was located in ER-derived protein bodies and was successfully purified by combining an aqueous-two phase partition system and a salting out step. Hydrophobin interactions allowed the formation of high molecular weight hemagglutinin structures, while unfused proteins were produced as monomers. Purified protein was shown to be biologically active and to induce neutralizing antibodies after mice immunization. Hydrophobin fusion to influenza hemagglutinin might therefore be a promising approach for rapid, easy, and low cost production of seasonal or pandemic influenza vaccines in plants. [less ▲]

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See detailHydropower: An Essential Partner of Renewable Energy Source
Lejeune, André ULg; Hui, Samuel; Pirotton, Michel ULg et al

in Sayigh, Ali (Ed.) World Renewable Energy Congress XI (2010, September 25)

In 2006, the 17 percent of the world’s electricity that was generated from hydropower represented nearly 90 percent of renewable electricity generation worldwide; Hydropower is an important source of ... [more ▼]

In 2006, the 17 percent of the world’s electricity that was generated from hydropower represented nearly 90 percent of renewable electricity generation worldwide; Hydropower is an important source of renewable energy and produces extremely small quantities of carbon dioxide. Between 1998 and 2008, renewable electricity production in the world rose from 2,794.9 to 3,762.6 TWh, i.e. an additional 967.6 TWh. As a final conclusion, despite hydropower’s high initial costs, its long-term overall costs tend to be low. The average cost of electricity production by hydropower is still very attractive for the moment. In the next future, hydropower will keep his position, in front of the other renewable sources of electricity, on the same level with biomass, better that wind power and much cheaper than solar energy. [less ▲]

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See detailHydrostatic flattening, core structure, and translational mode of the inner core
Denis, Carlo; Rogister, Yves; Amalvict, Martine et al

in Physics of the Earth & Planetary Interiors (1997), 99(3-4), 195-206

Modern Earth models are constrained by an inertia coefficient y*=J(2)/H approximate to 0.3308 which leads to a so-called 'hydrostatic' flattening f(hyd) approximate to 1/299.9. The latter is in rather ... [more ▼]

Modern Earth models are constrained by an inertia coefficient y*=J(2)/H approximate to 0.3308 which leads to a so-called 'hydrostatic' flattening f(hyd) approximate to 1/299.9. The latter is in rather large disagreement with the observed flattening f approximate to 1/298.3. We show that a more satisfactory value of the inertia coefficient for constraining a standard Earth model is y approximate to 0.332, consistent with a hydrostatic flattening of about 1/298.6. The change of the value of the inertia coefficient from about 0.331 to about 0.332 significantly alters the density structure of the core, notably the density jump at the inner core boundary. It brings, as a by-produce, the hydrostatic values of J(2) and H to better agreement with the observed values than is presently thought, and most probably leads for the Slichter mode to a period of much longer than 5.42 h, which is the value computed for PREM. [less ▲]

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See detailHydrothermal Pretreatments of Macroalgal Biomass for Biorefineries
Ruiz, Héctor A.; Rodríguez Jasso, Rosa M.; Aguedo, Mario ULg et al

in Prokop, Ales; Bajpai, Rakesh K.; Zappi, Mark E. (Eds.) Algal Biorefineries, Volume 2: Products and Refinery Design (2015)

Recently, macroalgal biomass is gaining wide attention as an alternative in the production of biofuels (as bioetanol and biogas) and compounds with high added value with specific properties (antioxidants ... [more ▼]

Recently, macroalgal biomass is gaining wide attention as an alternative in the production of biofuels (as bioetanol and biogas) and compounds with high added value with specific properties (antioxidants, anticoagulants, anti-inflammatories) for applications in food, medical and energy industries in accordance with the integrated biorefineries. Furthermore, biorefinery concept requires processes that allow efficient utilization of all components of the biomass. The pretreatment step in a biorefinery is often based on hydrothermal principles of high temperatures in aqueous solution. Therefore, in this chapter, a review on the application of hydrothermal pretreatment on macroalgal biomass is presented. [less ▲]

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See detailHydrothermal synthesis and crystal structure of Na(Na,Mn)(7)Mn-22(PO4)(18)0.5H(2)O, a new compound of fillowite structure type
Keller, Paul; Hatert, Frédéric ULg; Lissner, Falk et al

in European Journal of Mineralogy (2006), 18(6), 765-774

The chemical compound Na(Na,Mn)(7)Mn-22(PO4)(18)center dot 0.5H(2)O was synthesized with a Tuttle pressure vessel at 800 degrees C and 1 kbar during experimental investigations on the conditions of ... [more ▼]

The chemical compound Na(Na,Mn)(7)Mn-22(PO4)(18)center dot 0.5H(2)O was synthesized with a Tuttle pressure vessel at 800 degrees C and 1 kbar during experimental investigations on the conditions of fillowite formation. The crystal structure of a single-crystal, space group R (No. 148), a = 15.2741(9), c = 43.334(3) angstrom, Z = 6, was determined from X-ray intensity data and refined up to R-1 = 0.0728 and R-l{FO > 4 sigma(FO)} = 0.0546. The crystal structure of Na(Na,Mn)(7)Mn-22(PO4)(18)center dot 0.5H(2)O is similar to the structure of fillowite, Na6Ca3(Mn,Fe)(21)(PO4)(18.), but a significant difference between the synthetic H2O containing compound and fillowite is the replacement of (Na12)O-8(-) by Mn(H2O)(2)O-5(-) polyhedra, which form characteristic six-member rings. The coordination polyhedra around Na12 and Mn12 are so different that simple isomorphous substitution of Na12 by Mn12 is very unlikely, but whole six-member rings either of (Na12)O-8(-) or (Mn12)(H2O)(2)O-5 polyhedra seem to be distributed statistically over the crystal structure. No indication for ordering, e.g. sheet by sheet, has been observed. Na(Na,Mn)(7)Mn-22(PO4)(18)center dot 0.5H(2)O is the first fillowite-like compound for which water is located in the structure from single-crystal study. This result is proved by infrared spectral data and indicates that water can play an essential role in the fillowite structure-type. Structural features of the synthetic compounds Na(Na,Mn)(7)Mn-22(PO4)(18)center dot 0.5H(2)O, Na4Ca4Mg21(PO4)(18) and fillowite are compared. [less ▲]

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See detailHydrothermal Synthesis and Structure of Fe6.36Mn0.64(PO3(OH))4(PO4)2
Belfguira, Nadia; Walha, S.; Ben Salah, A. et al

in Journal of Chemical Crystallography (2010), 40

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See detailHydrothermal Synthesis and Structure of the Solid Solution (Fe0.54Mn0.46)(PO4)•2H2O
Belfguira, Nadia; Walha, S.; Kabadou, A. et al

in Journal of Chemical Crystallography (2011), 41

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See detailHydrothermal Synthesis of Alluaudite in the Na-Mn-Fe2+-Fe3+-P-O System
Hatert, Frédéric ULg

in Journal of Conference Abstracts (2002), 7(1), 44

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See detailHydrothermal synthesis, crystal chemistry, and stability of fillowite-type phosphates in the Na-Mn-Fe2+-Mg-PO4 system
Rondeux, Mélanie ULg; Hatert, Frédéric ULg

in Acta Mineralogica-Petrographica, Abstract series (2010), 6

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See detailHydrothermal transformations of triphylite from the Nanping No. 31 pegmatite dyke, Southeastern China.
RAO; Wang, R; Hatert, Frédéric ULg et al

in European Journal of Mineralogy (2014), 26

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See detailHydrotreatment of petroleum atmospheric residua using oceanic polymetallic nodules in trickle bed reactor
Dodet, Claude ULg; Noville, Francis ULg; Crine, Michel ULg et al

in Bulletin des Sociétés Chimiques Belges (1985), 94

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See detailHydroxyacids
PIERARD-FRANCHIMONT, Claudine ULg; QUATRESOOZ, Pascale ULg; PIERARD, Gérald ULg

in Cosmeceuticals-drugs vs cosmetics (2011)

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See detailHydroxychloroquine (Plaquenil) for recurrence prevention of Crohn's disease after curative surgery.
Louis, Edouard ULg; Belaiche, Jacques ULg

in Gastroentérologie Clinique et Biologique (1995), 19(2), 233-4

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See detailHydroxyethyl Starch 130/0.4 and the Risk of Acute Kidney Injury After Cardiopulmonary Bypass: A Single-Center Retrospective Study.
LAGNY, Marc-Gilbert ULg; ROEDIGER, Laurence ULg; KOCH, Jean-Noël ULg et al

in Journal of Cardiothoracic and Vascular Anesthesia (2015)

Hydroxyethyl Starches (HES) have been shown to increase the risk of acute kidney injury (AKI) in intensive care unit patients suffering sepsis. Whether this risk also applies to patients undergoing ... [more ▼]

Hydroxyethyl Starches (HES) have been shown to increase the risk of acute kidney injury (AKI) in intensive care unit patients suffering sepsis. Whether this risk also applies to patients undergoing elective surgery remains unclear. We investigated whether HES is associated with acute kidney injury after on-pump cardiac surgery. Materials and Methods: Balanced HES 130/0.4 (Volulyte®, Fresenius Kabi AG, Bad Homburg, Germany) was used as a pump prime and for intraoperative fluid therapy until July 2013 and has been entirely replaced by a balanced crystalloid solution (Plasmalyte®, Baxter, Lessines, Belgium) from August 2013. Data from 697 adult patients undergoing cardiac surgery between April 2013 and June 2014 were reviewed. HES patients were propensity-matched on previously published risk factors for AKI after cardiac surgery to patients treated with crystalloids. Postoperative AKI was defined as the primary outcome and assessed using the Acute Kidney Injury Network classification. A conditional logistic regression was used to investigate the association between the use of HES and postoperative AKI. Secondary outcomes included renal function at postoperative day 7, 30-day mortality, lengths of ICU and hospital stays and the incidence of postoperative respiratory complications. Results and Discussion: One hundred and thirty HES patients were successfully matched with 130 crystalloids patients. HES was significantly associated with postoperative AKI (odds ratio=2.4; 95 % CI= 1.2-4.8; P=0.02). No significant association was found between HES and any of the secondary outcomes. Conclusion: This study suggests that using balanced HES 130/0.4 as a pump prime and for intraoperative fluid therapy in adult patients undergoing on-pump cardiac surgery is associated with a higher incidence of AKI during the early postoperative period. [less ▲]

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See detailHydroxyl detection on Venus and Earth and implications for ozone
Migliorini; Piccioni, G.; Cardesin-Molino, A. et al

Poster (2009)

We present a comparison between hydroxyl night side infrared emissions on Earth and Venus. The vertical brightness distributions of the OH emissions are shown for the two planets and excitation processes ... [more ▼]

We present a comparison between hydroxyl night side infrared emissions on Earth and Venus. The vertical brightness distributions of the OH emissions are shown for the two planets and excitation processes are discussed. [less ▲]

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See detailThe Hydroxyl Nightglow Emissions on Earth, Venus and Mars
Soret, Lauriane ULg; Gérard, Jean-Claude ULg; Piccioni, G. et al

Poster (2015, August)

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See detailHydroxyl nightglow on Venus observed by VIRTIS on Venus-Express
Migliorini, A.; Piccioni, G.; Gérard, Jean-Claude ULg et al

Conference (2009, April)

Hydroxyl has been recently observed for the first time in the Venus atmosphere with the VIRTIS spectrometer on board the Venus-Express spacecraft. The (1-0) around 2.81 microns and the (2-0) around 1.46 ... [more ▼]

Hydroxyl has been recently observed for the first time in the Venus atmosphere with the VIRTIS spectrometer on board the Venus-Express spacecraft. The (1-0) around 2.81 microns and the (2-0) around 1.46 microns transitions have been detected. The intensity of the two emissions are respectively about 55 and 480 times less intense than the (0-0) oxygen transition (Piccioni et al, A&A, 2008). The possible chemical reactions which can produce hydroxyl on Venus involve O3 and HO2, with the former being the most probable, and hence OH can be used to indirectly infer the ozone distribution on Venus. VIRTIS data in limb mode observation were analyzed to derive the mean distribution of hydroxyl in the night side of Venus and the results are presented here. The typical peak altitude of the two emissions is set at 95-96 km in limb view, a few km lower than the oxygen emission at 1.27 um due to the transition (0-0). The peak altitude of the latter typically occurs at 97-98 km height. The OH full width at half maximum is in average about 7 km, and sometimes higher. The mean map of (1-0) hydroxyl distribution around 2.81 microns shows a maximum of emission of about 130 kR at about 1h local time. The study of the (2-0) hydroxyl distribution at around 1.46 microns results more difficult due to its weak intensity. [less ▲]

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See detailHydroxyl radical generation by new furocoumarin derivatives: a spin trapping and ESR study
Collet, M.; Hoebeke, Maryse ULg; Piette, J. et al

Conference (1993)

Detailed reference viewed: 8 (3 ULg)