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Peer Reviewed
See detailMechanochemical Study of Conformational Transitions in a Single Synthetic Peptide Chain
Willet, Nicolas ULg; Hinterdorfer, Peter; Lecommandoux, Sébastien et al

Poster (2012, June 05)

The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in ... [more ▼]

The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in detail by atomic force microscopy (AFM) at the single-molecule level. Synthetic copolymers containing a polypeptide block were prepared by N-carboxyanhydride amino acid ring-opening polymerization. The polymer chains were grafted as a dilute brush onto gold surfaces via disulfide end-groups. Their mechanochemical behavior was then studied by AFM single-molecule force spectroscopy (SMFS). The investigated polypeptide blocks were based on poly(L-glutamic acid), which undergoes a transition from alpha-helix to random coil. This can be induced by external stimuli (pH, ionic strength, temperature) or simply by applying a force. We were able to study the mechanically driven unfolding of the peptide by stretching-release cycles of the biomacromolecule. Stretching the helical peptide resulted in original features in the force-distance traces. Plateaus that are specific for the helical conformation were detected, quantified and discussed. Pulling-relaxing SMFS experiments eventually led to a better understanding of the force induced unfolding of an alpha-helix and the reversibility of the phenomenon. [less ▲]

Detailed reference viewed: 56 (0 ULg)
See detailMechanochemistry of a single polypeptide molecule: Study of force-induced conformational transitions
Willet, Nicolas ULg; Hinterdorfer, Peter; Lecommandoux, Sébastien et al

Conference (2011, August)

The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in ... [more ▼]

The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in detail by atomic force microscopy (AFM) at the single-molecule level. Synthetic copolymers containing a polypeptide block were prepared by N-carboxyanhydride amino acid ring-opening polymerization. The polymer chains were grafted as a dilute brush onto gold surfaces via disulfide end-groups. Their mechanochemical behavior was then studied by AFM single-molecule force spectroscopy (SMFS). The investigated polypeptide blocks were based on poly(L-glutamic acid), which undergoes a transition from alpha-helix to random coil. This can be induced by external stimuli (pH, ionic strength, temperature) or simply by applying a force. We were able to study the mechanically driven unfolding of the peptide by stretching-release cycles of the biomacromolecule. Stretching the helical peptide resulted in original features in the force-distance traces. Plateaus that are specific for the helical conformation were detected, quantified and discussed. Pulling-relaxing SMFS experiments eventually led to a better understanding of the force induced unfolding of a alpha-helix and the reversibility of the phenomenon. [less ▲]

Detailed reference viewed: 57 (2 ULg)
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See detailMechanochemistry: targeted delivery of single molecules
Duwez, Anne-Sophie ULg; Cuenot, Stéphane; Jérôme, Christine ULg et al

in Nature Nanotechnology (2006), 1

The use of scanning probe microscopy-based techniques to manipulate single molecules(1) and deliver them in a precisely controlled manner to a specific target represents a significant nanotechnological ... [more ▼]

The use of scanning probe microscopy-based techniques to manipulate single molecules(1) and deliver them in a precisely controlled manner to a specific target represents a significant nanotechnological challenge(2,3). The ultimate physical limit in the design and fabrication of organic surfaces can be reached using this approach. Here we show that the atomic force microscope (AFM), which has been used extensively to investigate the stretching of individual molecules(4-12), can deliver and immobilize single molecules, one at a time, on a surface. Reactive polymer molecules, attached at one end to an AFM tip, are brought into contact with a modified silicon substrate to which they become linked by a chemical reaction. When the AFM tip is pulled away from the surface, the resulting mechanical force causes the weakest bond - the one between the tip and polymer - to break. This process transfers the polymer molecule to the substrate where it can be modified by further chemical reactions. [less ▲]

Detailed reference viewed: 79 (42 ULg)
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See detailMechanochemistry: targeted delivery of single molecules
Duwez, Anne-Sophie ULg; Cuenot, Stéphane; Jérôme, Christine ULg et al

in Nature Nanotechnology (2006), 1

The use of scanning probe microscopy-based techniques to manipulate single molecules1 and deliver them in a precisely controlled manner to a specific target represents a significant nanotechnological ... [more ▼]

The use of scanning probe microscopy-based techniques to manipulate single molecules1 and deliver them in a precisely controlled manner to a specific target represents a significant nanotechnological challenge. The ultimate physical limit in the design and fabrication of organic surfaces can be reached using this approach. Here we show that the atomic force microscope (AFM), which has been used extensively to investigate the stretching of individual molecules, can deliver and immobilize single molecules, one at a time, on a surface. Reactive polymer molecules, attached at one end to an AFM tip, are brought into contact with a modified silicon substrate to which they become linked by a chemical reaction. When the AFM tip is pulled away from the surface, the resulting mechanical force causes the weakest bond — the one between the tip and polymer — to break. This process transfers the polymer molecule to the substrate where it can be modified by further chemical reactions [less ▲]

Detailed reference viewed: 10 (4 ULg)
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See detailMechanoreceptors in the anterior horn of the equine medial meniscus: an immunohistochemical approach.
Nemery, Elodie ULg; Gabriel, Annick ULg; Piret, Joëlle ULg et al

Poster (2014)

Mechanoreceptors are “encapsulated sensory end-organs” involved in proprioceptive function. Given the high incidence of meniscal injuries in horses, the clinical interest in these mechanoreceptors ... [more ▼]

Mechanoreceptors are “encapsulated sensory end-organs” involved in proprioceptive function. Given the high incidence of meniscal injuries in horses, the clinical interest in these mechanoreceptors, particularly in the meniscus, and the lack of information concerning them in equine menisci, our objective was to study these corpuscles in the anterior horn of the equine medial meniscus, which is the most common localization reported for equine meniscal injuries. An immunohistochemical approach to detect Schwann cells and nerve fibres allowed us to localize and identify these corpuscles within the meniscus. Three types of mechanoreceptors were identified and localized between the abaxial quarter and the abaxial third of the meniscus: the Ruffini, Pacini and Golgi corpuscles. In conclusion, from a purely fundamental point of view, our work highlights for the first time the presence of MCR at the level of the anterior horn of the equine medial meniscus and proposes a classification based on specific immunocytochemical techniques. This morphological approach could serve as a basis for clinical studies, in order to evaluate the impact of these corpuscles on the poor sportive prognosis in equine meniscal tears. [less ▲]

Detailed reference viewed: 29 (6 ULg)
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See detailThe mechanosensitivity of cells in joint tissues: Role in the pathogenesis of joint diseases
Sanchez, Christelle ULg; Mathy, Marianne ULg; Henrotin, Yves ULg

in Kamkin, André; Kiseleva, Irina (Eds.) Mechanosensitivity and Mechanotransduction (2011)

Detailed reference viewed: 20 (8 ULg)
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Peer Reviewed
See detailMeckel's diverticulum as a cause of colic: 2 cases with different morphological features
Verwilghen, Denis ULg; Van Galen, Gaby ULg; Busoni, Valeria ULg et al

in Tijdschrift voor Diergeneeskunde (2010), 135(11), 452-455

Detailed reference viewed: 57 (13 ULg)
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See detailMECOSIG adapted to the design of distributed GIS
Pasquasy, Fabien; Laplanche, François; Sainte, Jean-Christophe et al

in Lecture Notes in Computer Science (2005), 3762

For more than ten years MECOSIG has been used as a method for GIS design and implementation in various national and international projects achieved in our laboratory. During a decade, the method has been ... [more ▼]

For more than ten years MECOSIG has been used as a method for GIS design and implementation in various national and international projects achieved in our laboratory. During a decade, the method has been progressively improved and extended without modification of its basic principles. However the emergence of distributed GIS, implying several organizations capable to play various roles, requires the reappraisal of the methodology. New concerns are identified and a collection of new tools must be deployed. Taking the most of various recent researches completed for public authorities in Belgium, this paper presents some significant adaptations of the original MECOSIG method in order to cope with a distributed GIS environment. [less ▲]

Detailed reference viewed: 30 (0 ULg)
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See detailMECOSIG Adapted to the Design of Distributed GIS
Pasquasy, Fabien; Laplanche, François; Sainte, Jean-Christophe et al

in OTM 2005 Workshops (2005)

Detailed reference viewed: 23 (3 ULg)
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See detailMedecin de famille et intoxication au monoxyde de carbone
Burette, Philippe ULg; Bouüaert, Corine ULg; Vanmeerbeek, Marc ULg et al

in Revue Médicale de Liège (2006), 61(5-6, May-Jun), 285-90

Carbon monoxide poisoning is not easily identifiable. It is the first cause of death by accidental poisoning in Europe. The family practitioner, who has not been made aware of this problem, incurs the ... [more ▼]

Carbon monoxide poisoning is not easily identifiable. It is the first cause of death by accidental poisoning in Europe. The family practitioner, who has not been made aware of this problem, incurs the risk of diagnostic indecision or of involuntary personal poisoning. Since symptomatology is non specific, the general practitioner answering housecalls is sometimes confronted with an urgent medical problem linked to the complications of this intoxication (coronary, neurological problems...), without having ways of documenting its origin of the poisoning or any means to protect himself. Through direct contact with his patients' environment, the family practitioner, being made sensitive to this problem, can certainly contribute to care and aftercare of the patient suffering from carbon monoxide poisoning, but also to the prevention of this public health problem often called "the silent killer". [less ▲]

Detailed reference viewed: 165 (12 ULg)
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See detailLe médecin de famille et l'hôpital de jour: complémentarité de soins
Triffaux, Jean-Marc ULg; Halkin, Stéphane ULg; Bertrand, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(3), 133-40

Detailed reference viewed: 29 (2 ULg)
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See detailLe médecin du travail face au harcèlement moral au travail : constats et prespectives
Gruslin, Isabelle ULg; Italiano, Patrick ULg; Faulx, Daniel ULg

in Médecine du Travail & Ergonomie= Arbeidsgezondheidszorg & Ergonomie (2002), 39(2), 61-68

L'article de D. Faulx et C. Geuzaine, paru dans le numéro 3-2000 de cette revue, aborde l'étendue du phénomène du harcèlement moral ainsi que les aspects juridiques de la définition, la description du ... [more ▼]

L'article de D. Faulx et C. Geuzaine, paru dans le numéro 3-2000 de cette revue, aborde l'étendue du phénomène du harcèlement moral ainsi que les aspects juridiques de la définition, la description du processus, ses conséquences médicales et les facteurs de risque. Dans la suite de cette étude, nous présentons ici les résultats d'une enquête menée auprès des médecins du travail, en tant que témoins et intervenants privilégiés dans le domaine du harcèlement. Cette enquête vise à apporter des informations descriptives originales concernant les attitudes, les opinions et représentations des médecins du travail par rapport au phénomène de harcèlement moral. En outre, elle est l'occasion de tester une série d'hypothèses sur les facteurs pouvant influencer l'attitude des médecins du travail vis-à-vis du harcèlement moral. [less ▲]

Detailed reference viewed: 174 (14 ULg)
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See detailLe médecin en promotion de la santé à l'école - Actes des ateliers
Vandoorne, Chantal ULg; Melen, Geoffroy ULg; Saglimbene, Concetta ULg et al

Scientific conference (2009, November)

Detailed reference viewed: 40 (23 ULg)
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See detailLe médecin et le livre
Marganne, Marie-Hélène ULg

in Gourevitch, Danielle (Ed.) Pour une archéologie de la médecine romaine (2011)

Detailed reference viewed: 11 (0 ULg)
See detailLe médecin généraliste et l'éducation pour la santé
Gosset, Christiane ULg; Reginster-Haneuse, G.

in Actes du congrès "Prévention et médecine générale" (1991)

Detailed reference viewed: 4 (1 ULg)
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Peer Reviewed
See detailLe Médecin Généraliste face à l'hypothyroïdie fruste périphérique
Loor, Michel; Giet, Didier ULg

in Revue Médicale de Liège (2008), 63(10), 600-608

Detailed reference viewed: 64 (8 ULg)
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See detailLe médecin généraliste face à l'urgence dentaire.
Dabée, Jean-Claude ULg; Geerts, Sabine ULg; Charpentier, Joseph ULg

in Revue Médicale de Liège (2000), 55(12), 1022-7

Counselling and drug prescription are the only means for the phycisian to manage dental emergencies. Among these, some are infection related: pulpitis, apical and periodontal abscesses, acute necrotizing ... [more ▼]

Counselling and drug prescription are the only means for the phycisian to manage dental emergencies. Among these, some are infection related: pulpitis, apical and periodontal abscesses, acute necrotizing ulcerative gingivitis and alveolitis. Urgent medical treatment includes antibiotics, mouth rinses and analgesics except for pulpitis (pain caused by thermal stimuli) for which analgesics alone are prescribed. Infection non related dental emergencies are: hemorrhage for which the best treatment is compression; trauma, mainly dental avulsion for which reimplantation must be done as soon as possible; acute TMD such as open lock which can be reduced by the Nelaton handling. [less ▲]

Detailed reference viewed: 220 (5 ULg)