Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailIdentification of chromosomal regions associated with segregation distortion of SSR markers and the genes controlling the low-gossypol seed & high-gossypol plant trait of Gossypium sturtianum
Benbouza, H.; Diouf, F.B.; Schefflers, J. et al

Conference (2010)

Distorted segregation of DNA markers is commonly encountered, especially in inter-specific crosses. Our main objective in this study was to identify chromosomal regions consistently associated with ... [more ▼]

Distorted segregation of DNA markers is commonly encountered, especially in inter-specific crosses. Our main objective in this study was to identify chromosomal regions consistently associated with segregation distortion in [(G. hirsutum x G. raimondii) x G. sturtianum] (HRS) hybrid. Segregation distortion skews the genotypic frequencies from their Mendelian expectations. In HRS progeny, chi square analysis (P < 0.01) showed significant skewed in all targeted linkage groups c2-c14, c3-c17, and c6-c25. Chromosomal region was regarded as being associated with skewed segregation, if three or more closely linked markers exhibited significant segregation distortion in one or more population(s). The targeted introgression regions in the tested population seem to be favourable for segregation distortion. Segregation distortion in HRS hybrid progenies differed in male and female gametes. Furthermore, the data indicated that the environment has strongly influenced the transfer of SSR markers through microspores. The consistent location of these chromosomal regions in selfed and backcross of HRS derivatives indicate probably the identification of segregation distortion regions (SDRs) in HRS hybrid. Comparison with results regarding the segregation distortion regions obtained in previous research by other authors and results we obtained regarding the absence of recombinations between BNL3436 and BNL1153 markers mapped on c6-c25 chromosome and spanned by 64 cM on the G. hirsutum map, after several generations of selfing, arise the question of the conservation of the gene order and spacing in G. sturtianum. Results showed that three SSR markers mapped on c6-c25 linkages groups were systematically transmitted in all selected progenies of the HRS tri-specific hybrid. Furthermore, the high percentages of loci with significant segregation distortion observed in this study suppose that a genetic mechanism may exist for preferential transmission of alien chromosomes segments. High heterozygosity frequencies (+/- 80%) were observed for all conserved G. sturtianum SSR markers, after several generations of backcrossing and selfing, which indicate that the cytogenetic and genetic conditions for obtaining homozygotes at high frequency are not met. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailIdentification of cis-regulatory elements controlling two differentially expressed Pit-1 genes in the duplicated genome of Cyprinus carpio
Kausel, G. M.; Castro, L. A.; Vera, T. S. et al

in FEBS Journal (2005, July), 272(Suppl. 1), 469-470

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailIdentification of clinical parameters predictive of one-year survival using two geriatric tools in clinically fit older patients with hematological malignancies: Major impact of cognition
Dubruille, Stéphanie; Libert, Yves; Roos, Myriam et al

in Journal of Geriatric Oncology (2015), 6(5), 362-369

Background Little is known about the reliability of G8 screening tool and the prognostic value of clinical parameters within the Comprehensive Geriatric Assessment (CGA) in clinically fit older patients ... [more ▼]

Background Little is known about the reliability of G8 screening tool and the prognostic value of clinical parameters within the Comprehensive Geriatric Assessment (CGA) in clinically fit older patients with hematological malignancies. Materials and Methods This study was performed to assess the reliability of G8 as a screening tool and to determine the predictive value of CGA items in terms of 1-year overall survival (OS). G8 and CGA were proposed to 107 consecutive patients (65–89 years) with hematological malignancies assessed by their physicians as clinically fit, meaning not exhibiting geriatric syndromes and/or irreversible comorbidities significantly impairing their daily function, and thus able to receive chemotherapy. Results Out of 107 patients, 90 patients were evaluable and completed both scales; 72% and 80% were defined as “vulnerable” when evaluated with G8 (≤14.5) or CGA (≥2 impairments) respectively. The area under ROC-curve of G8 compared to CGA was 0.749 ± 0.051. Neither G8 nor CGA total scores were predictive of 1-year OS. However, age (HR = 1.105, 95% CI: 1.016–1.202; p = 0.019), diagnosis (HR = 5.208, 95% CI: 1.895–14.310; p = 0.001) and cognitive status (HR = 3.260, 95% CI: 1.043–10.194; p = 0.042) were predictive of OS. Conclusions We conclude that in our selected hematological patients: 1) the G8 score does not help selecting patients for CGA, 2) the G8 and CGA total scores do not predict OS, and 3) in addition to the age and disease itself, cognitive impairment appears to be a powerful prognostic factor. [less ▲]

Detailed reference viewed: 45 (1 ULg)
Full Text
Peer Reviewed
See detailIdentification of Clostridium tyrobutyricum and related species using sugar fermentation, organic acid formation and DNA probes based on 16S rRNA sequences
Klijn, N.; Bovie, C.; Dommes, Jacques ULg et al

in Systematic and Applied Microbiology (1994), 17(2), 249-256

Detailed reference viewed: 19 (1 ULg)
Peer Reviewed
See detailIdentification of coiled body-like structures in meristematic cells of Pisum sativum cotyledonary buds.
Jennane, A.; Thiry, Marc ULg; Goessens, Guy ULg

in Chromosoma (1999), 108(2), 132-42

This study focused on two types of nuclear bodies visible in plant cells that were previously thought to be similar to the coiled bodies (CBs) of animal cells: the nucleolus-associated body (NAB) and ... [more ▼]

This study focused on two types of nuclear bodies visible in plant cells that were previously thought to be similar to the coiled bodies (CBs) of animal cells: the nucleolus-associated body (NAB) and dense body (DB). We show that both NABs and DBs share common features with animal CBs: they consist of ribonucleoproteins, are silver-stainable, and lack DNA. Immunoelectron microscopy shows that only the NABs are rich in snRNAs and fibrillarin, two markers characteristic of animal CBs. This suggests that NABs rather than DBs are the plant counterparts of the CBs of animal cells. These structures appear most frequently in cells blocked in G0-1, their frequency gradually declining with resumption of the cell cycle and nucleolar activity. During this reactivation period, NABs are released from the nucleolus to the nucleoplasm, suggesting that they may act as nuclear transport or sorting structures. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailIdentification of COX-2 selective inhibitor
Michaux, C.; Julémont, F.; de Leval, X. et al

in Fundamental & Clinical Pharmacology (2004)

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailIdentification of cyclooxygenase-2 as a major actor of the transcriptomic adaptation of endothelial and tumor cells to cyclic hypoxia: effect on angiogenesis and metastases.
Daneau; Boidot; MARTINIVE, Philippe ULg et al

in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (2010), 16(2), 410-419

Purpose: Cyclic hypoxia in tumors originates from heterogeneities in RBC flux and influences not only tumor cells but also endothelial cells lining tumor blood vessels. Whether pO2 fluctuations ... [more ▼]

Purpose: Cyclic hypoxia in tumors originates from heterogeneities in RBC flux and influences not only tumor cells but also endothelial cells lining tumor blood vessels. Whether pO2 fluctuations, particularly transient reoxygenation periods, alter the well-known hypoxia-inducible factor (HIF)–dependent gene program is largely unknown. Experimental Design: We compared the transcriptomic profiles of endothelial and tumor cells exposed to cyclic hypoxia versus continuous hypoxia to uncover a possible differential effect on angiogenesis and metastases. Results: Microarray analyses identified early genes that were selectively induced by cyclic hypoxia in endothelial cells. Among them, we focused on PTGS2 because the observed increase in mRNA expression led to a significant increase in the expression and activity of cyclooxygenase-2 (COX-2; the protein product of PTGS2). HIF-1α was shown to be stabilized by cyclic hypoxia (despite reoxygenation periods) and to favor COX-2 induction as validated by the use of echinomycin and HIF-1α targeting small interfering RNA. Using a specific COX-2 inhibitor and a dedicated COX-2 targeting small interfering RNA, we documented that COX-2 accounted for the higher endothelial cell survival and angiogenic potential conferred by cyclic hypoxia. Cyclic hypoxia also led to a preferential COX-2 induction in tumor cells and, contrary to continuous hypoxia, fostered a higher metastatic take of prechallenged tumor cells. Conclusions: Our study documents that PTGS2/COX-2 is part of a cyclic hypoxia gene signature and largely accounts for the unique phenotype of endothelial and tumor cells exposed to fluctuations in pO2, thereby offering new perspectives for the clustering of tumors expressing COX-2 together with other cyclic hypoxia-responsive genes. Clin Cancer Res; 16(2); 410–9 [less ▲]

Detailed reference viewed: 55 (8 ULg)
Peer Reviewed
See detailIdentification Of Cystosolic Mg2+-Dependent Soluble Inorganic Pyrophosphatases In Potato And Phylogenetic Analysis
Rojas-Beltran, Ja.; Dubois, Françoise ULg; Mortiaux, F. et al

in Plant Molecular Biology (1999), 39(3),

Detailed reference viewed: 27 (5 ULg)
Full Text
Peer Reviewed
See detailIdentification of Cytotoxic and Antioxidant Compounds from Allium gramineum Flowers
Mskhiladze, Lasha; Chincharadze, David; Tits, Monique ULg et al

in International Journal of Pharmaceutical Sciences and Drug Research (2015), 7

The present study evaluates the in vitro anticancer, antiplasmodial and antioxidant activity of the ethanolic crude extract from the flowers of Allium gramineum growing in Georgia and of one flavonol and ... [more ▼]

The present study evaluates the in vitro anticancer, antiplasmodial and antioxidant activity of the ethanolic crude extract from the flowers of Allium gramineum growing in Georgia and of one flavonol and two steroidal glycosides which were isolated from this plant. The flowers were extracted with ethanol and this total extract was subjected to successive bioguided fractionations to provide glycosides 1-3. Their structures were elucidated on the basis of NMR and ESI-MS spectrometric data in comparison with the existing literature and have been identified as: isorhamnetin-3-O-β-D-glucopyranoside (1), diosgenin-3-O-α-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (Prosapogenin A of dioscin) (2), diosgenin-3-O-α-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)-]-β-D-glucopyranoside (Deltonine) (3). The ethanolic extract has been shown to strongly inhibit the growth of breast adenocarcinoma cell lines, with an IC50 of 4.5 ± 0.7μg/mL for MDAMB-231 and 4.8 ± 0.9μg/mL for MCF-7 cells. The cytotoxic activity was related to 2 and 3 which exhibited potent cytotoxicity, with an IC50 of ± 3μM. Concerning antiplasmodial activities, only weak activities were observed using the ethanolic extract and the two saponins. The flavonoid was almost inactive. Finally, the radical-scavenging activity of the ethanolic extract was tested in presence of ABTS·+ solution. A decrease of the absorbance intensity was observed, with an IC50 value of 22.1 ± 0.6μg/mLwhile trolox, used as Standard drug, showed a pronounced activity (IC50 = 12.7±0.5μM). The glycoside 1 showed the lowest IC50 value of 20.1 ± 0.8μM while both 2 and 3 exhibited very weak radical scavenging activity. [less ▲]

Detailed reference viewed: 64 (13 ULg)
Peer Reviewed
See detailIdentification of cytotoxic factors in media conditioned by mouse B16 melanoma cells
Siwek, Brigitte; De Pauw-Gillet, Marie-Claire ULg; Sabbioni, Enrico et al

in Anticancer Research (1990), 10(5B), 1390

Detailed reference viewed: 6 (0 ULg)
Full Text
See detailIdentification of Deep sea Flora on Video Imagery
Carré, Cyril ULg

Scientific conference (2010, May)

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailIdentification of Different Target Glycoproteins for Bovine Herpes Virus Type 1-Specific Cytotoxic T Lymphocytes Depending on the Method of in Vitro Stimulation
Denis, M.; Slaoui, M.; Keil, G. et al

in Immunology (1993), 78(1), 7-13

Vaccinia virus recombinants expressing the three major bovine herpes virus-1 (BHV-1) glycoproteins gI, gIII and gIV were used to identify the major target antigens for BHV-1-specific CTL isolated from ... [more ▼]

Vaccinia virus recombinants expressing the three major bovine herpes virus-1 (BHV-1) glycoproteins gI, gIII and gIV were used to identify the major target antigens for BHV-1-specific CTL isolated from immune cattle. Peripheral blood mononuclear cells (PBMC) expanded in vitro in the presence of interleukin-2 (IL-2) and lysed both gIII- and gIV-infected target cells. Secondary in vitro stimulation of PBMC was also performed in the presence of either fixed BHV-1-infected autologous fibroblasts or ultraviolet (UV)-inactivated virus. Both methods of antigen presentation allowed the proliferation of BHV-1-specific CTL but the target glycoprotein for these CTL differed depending on the method of stimulation. Vaccinia-gIV-infected targets were lysed predominantly when PBMC were stimulated by fixed infected fibroblasts, whilst PBMC stimulated by UV-inactivated virus lysed mostly vaccinia-gIII-infected targets. This observation could be explained by a different processing pathway of BHV-1 antigens in each cell type involved. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
See detailIdentification of differential item functioning by logistic regression: application to intellectual disability data
Magis, David ULg; Facon, Bruno

Conference (2010, October 14)

The purpose of this talk is to briefly present the logistic regression procedure to identify differential itm functioning (DIF) in psychometrics, and to illustrate the method on a practical data set about ... [more ▼]

The purpose of this talk is to briefly present the logistic regression procedure to identify differential itm functioning (DIF) in psychometrics, and to illustrate the method on a practical data set about intellectual disability. DIF is an important research fi eld and many statistical methods have been developed in this framework. Logistic regression has the asset of not being based on item response models, and it can detect both uniform and nonuniform DIF. In addition, it can be applied to the comparison of more than two groups. This method is illustrated by comparing two groups of French pupils, one group of normal ability students and one group with intellectually disabled students. Raven's Colored Progressive Matrices test is assigned and DIF is investigated between the two groups. It is concluded that none of the items can be flagged as functioning differently. [less ▲]

Detailed reference viewed: 29 (4 ULg)
Full Text
Peer Reviewed
See detailIdentification of differential item functioning in multiple-group settings: A multivariate outlier detection approach
Magis, David ULg; De Boeck, Paul

in Multivariate Behavioral Research (2011), 46

This paper focuses on the identification of differential item functioning (DIF) when more than two groups of examinees are considered. We propose to consider items as elements of a multivariate space ... [more ▼]

This paper focuses on the identification of differential item functioning (DIF) when more than two groups of examinees are considered. We propose to consider items as elements of a multivariate space, where DIF items are outlying elements. Following this approach, the situation of multiple groups is a quite natural case. A robust statistics technique is proposed to identify DIF items as outliers in the multivariate space. For low dimensionalities, up to two three groups, also a simple graphical tool is derived. We illustrate our approach with a re-analysis of data from Kim, Cohen, and Park (1995) on using calculators for a mathematics test. [less ▲]

Detailed reference viewed: 45 (5 ULg)
Full Text
Peer Reviewed
See detailIdentification of differential pattern of protein expression in canine osteoarthritis serum after anterior cruciate ligament transection: a proteomic analysis.
Gharbi, Myriam; Sanchez, Christelle ULg; Mazzucchelli, Gabriel ULg et al

in Veterinary journal (London, England : 1997) (2013), 197(3), 848-53

Osteoarthritis (OA) management remains a great challenge and there is considerable effort to understand its pathophysiology and to identify new therapeutic targets and biomarkers. Canine OA surgically ... [more ▼]

Osteoarthritis (OA) management remains a great challenge and there is considerable effort to understand its pathophysiology and to identify new therapeutic targets and biomarkers. Canine OA surgically induced by the transection of the anterior cruciate ligament (ACLT) is a widely used and relevant model. This study reports a proteome mapping of dog serum and an analysis of the differentially expressed proteins between before and after ACLT. In the first part of the study, 261 picked protein spots were identified from preparative 2D gels and 71 different proteins were identified among the 261 spots present on the reference map. Canine serum proteome mapping reveals the presence of proteins of interest, such as fetuin B, complement C3 and C1s and pregnancy zone protein. The comparison between serum from dogs before and after ACLT reveals the differential expression of several proteins that could play a key role in the pathogenesis of OA. A number of proteins, such as fetuin B and complement C3, were increased in dog OA serum whereas others, such as hyaluronan binding protein 2, inter-alpha-trypsin inhibitor H4 (ITIH4), complement C1s and C4 and haptoglobin were decreased. Some of these proteins could be candidate biomarkers for diagnosis, prognosis and treatment evaluation. The results of the study also reinforced the similarities between dog experimental OA and human cases of OA. [less ▲]

Detailed reference viewed: 16 (1 ULg)
Full Text
Peer Reviewed
See detailIdentification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis.
Toro, Sabrina; Wegner, Jeremy; Muller, Marc ULg et al

in Gene Expression Patterns (2009), 9(4), 200-8

The vertebrate hypothalamic-pituitary axis (HP) is the main link between the central nervous system and endocrine system. Although several signal pathways and regulatory genes have been implicated in ... [more ▼]

The vertebrate hypothalamic-pituitary axis (HP) is the main link between the central nervous system and endocrine system. Although several signal pathways and regulatory genes have been implicated in adenohypophysis ontogenesis, little is known about hypothalamic-neurohypophysial development or when the HP matures and becomes functional. To identify markers of the HP, we constructed subtractive cDNA libraries between adult zebrafish hypothalamus and pituitary. We identified previously published genes, ESTs and novel zebrafish genes, some of which were predicted by genomic database analysis. We also analyzed expression patterns of these genes and found that several are expressed in the embryonic and larval hypothalamus, neurohypophysis, and/or adenohypophysis. Expression at these stages makes these genes useful markers to study HP maturation and function. [less ▲]

Detailed reference viewed: 25 (5 ULg)
Full Text
Peer Reviewed
See detailIdentification Of Distinct Potyviruses In Mixedly-Infected Sweet-Potato By The Polymerase Chain-Reaction With Degenerate Primers
Colinet, D.; Kummert, J.; Lepoivre, Philippe ULg et al

in Phytopathology (1994), 84(1),

Detailed reference viewed: 20 (0 ULg)