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See detailKinetic study of the transport of adenine nucleotides into rat heart mitochondria (proceedings)
Sluse, Francis ULg; Duyckaerts, Claire ULg; Sluse-Goffart, Claudine et al

in Archives Internationales de Physiologie et de Biochimie (1978), 86

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See detailKinetic Study of Two Novel Enantiomeric Tricyclic Beta-Lactams Which Efficiently Inactivate Class C Beta-Lactamases
Vilar, M.; Galleni, Moreno ULg; Solmajer, T. et al

in Antimicrobial Agents and Chemotherapy (2001), 45(8), 2215-23

A detailed kinetic study of the interaction between two ethylidene derivatives of tricyclic carbapenems, Lek 156 and Lek 157, and representative beta-lactamases and D-alanyl-D-alanine peptidases (DD ... [more ▼]

A detailed kinetic study of the interaction between two ethylidene derivatives of tricyclic carbapenems, Lek 156 and Lek 157, and representative beta-lactamases and D-alanyl-D-alanine peptidases (DD-peptidases) is presented. Both compounds are very efficient inactivators of the Enterobacter cloacae 908R beta-lactamase, which is usually resistant to inhibition. Preliminary experiments indicate that various extended-spectrum class C beta-lactamases (ACT-1, CMY-1, and MIR-1) are also inactivated. With the E. cloacae 908R enzyme, complete inactivation occurs with a second-order rate constant, k(2)/K', of 2 x 10(4) to 4 x 10(4) M(-1) s(-1), and reactivation is very slow, with a half-life of >1 h. Accordingly, Lek 157 significantly decreases the MIC of ampicillin for E. cloacae P99, a constitutive class C beta-lactamase overproducer. With the other serine beta-lactamases tested, the covalent adducts exhibit a wide range of stabilities, with half-lives ranging from long (>4 h with the TEM-1 class A enzyme), to medium (10 to 20 min with the OXA-10 class D enzyme), to short (0.2 to 0.4 s with the NmcA class A beta-lactamase). By contrast, both carbapenems behave as good substrates of the Bacillus cereus metallo-beta-lactamase (class B). The Streptomyces sp. strain R61 and K15 extracellular DD-peptidases exhibit low levels of sensitivity to both compounds. [less ▲]

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See detailKinetic study on a Fe-Co/Al2O3 catalyst for carbon nanotube synthesis by CCVD process
Pirard, Sophie ULg; Douven, Sigrid ULg; Bosuot, Christophe et al

Poster (2006, April)

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See detailKinetic theory of dust-acoustic waves in plasmas with dust distributions and charge fluctuations
Cattaert, Tom ULg; Verheest, F.

in IEEE Transactions on Plasma Science (2004), 32(2), 537-541

Dust-acoustic waves in dusty plasmas are studied in a kinetic context incorporating dust distributions and charge fluctuations, two of the main features that distinguish dusty or complex from ordinary ... [more ▼]

Dust-acoustic waves in dusty plasmas are studied in a kinetic context incorporating dust distributions and charge fluctuations, two of the main features that distinguish dusty or complex from ordinary plasmas. This is done by deriving a generalized Vlasov equation in an extended phase space. No specific charging model is used so that the results are generic. [less ▲]

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See detailKinetics and diffusional limitations in nanostructured heterogeneous catalyst with controlled pore texture
Pirard, Sophie ULg; Diverchy, Chantal; Hermans, Sophie et al

in Catalysis Communications (2011), 12

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See detailKinetics and diffusional limitations in nanostructured heterogeneous catalyst with controlled pore texture
Pirard, Sophie ULg; Hermans, Sophie; Devillers, Michel et al

Poster (2011, September)

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See detailKinetics and electron spin resonance study of the radical polymerization of n-butyl acrylate mediated by a nitroxide precursor: C-phenyl-N-tert-butylnitrone
Sciannamea, Valérie; Bernard, Maxime; Catala, Jean-Marie et al

in Journal of Polymer Science. Part A, Polymer Chemistry (2006), 44(21), 6299-6311

The C-phenyl-N-tert-butylnitrone/azobisisobutyronitrile pair is able to impart control to the radical polymerization of n-butyl acrylate as long as a two-step process is implemented, that is, the ... [more ▼]

The C-phenyl-N-tert-butylnitrone/azobisisobutyronitrile pair is able to impart control to the radical polymerization of n-butyl acrylate as long as a two-step process is implemented, that is, the prereaction of the nitrone and the initiator in toluene at 85 degrees C for 4 h followed by the addition and polymerization of n-butyl acrylate at 110 degrees C. The structure of the in situ formed nitroxide has been established from kinetic and electron spin resonance data. The key parameters (the dissociation rate constant, combination rate constant, and equilibrium constant) that govern the process have been evaluated. The equilibrium constant between the dormant and active species is close to 1.6 x 10(-12) mol L-1 at 110 degrees C. The dissociation rate constant and the activation energy for the C-ON bond homolysis are 1.9 x 10(-3) s(-1) and 122 +/- 15 kJ mol(-1), respectively. The rate constant of recombination between the propagating adical and the nitroxide is as high as 1.2 x 10(9) L mol(-1) s(-1). Finally, well-defined poly(n-butyl acrylate)-b-polystyrene block copolymers have been successfully prepared. [less ▲]

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See detailKinetics and energetics of ligand binding determined by microcalorimetry: Insights into active site mobility in a psychrophilic alpha-amylase
D'Amico, Salvino ULg; Sohier, Jean ULg; Feller, Georges ULg

in Journal of Molecular Biology (2006), 358(5), 1296-1304

A new microcalorimetric method for recording the kinetic parameters k(cat)/K-m and K-i of alpha-amylases using polysaccharides and oligosaccharides as substrates is described. This method is based on the ... [more ▼]

A new microcalorimetric method for recording the kinetic parameters k(cat)/K-m and K-i of alpha-amylases using polysaccharides and oligosaccharides as substrates is described. This method is based on the heat released by glycosidic bond hydrolysis. The method has been developed to study the active site properties of the cold-active alpha-amylase produced by an Antarctic psychrophilic bacterium in comparison with its closest structural homolog from pig pancreas. It is shown that the psychrophilic a-amylase is more active on large macromolecular substrates and that the higher rate constants k(cat) are gained at the expense of a lower affinity for the substrate. The active site is able to accommodate larger inhibitory complexes, resulting in a mixed-type inhibition of starch hydrolysis by maltose. A method for recording the binding enthalpies by isothermal titration calorimetry in a low-affinity system has been developed, allowing analysis of the energetics of weak ligand binding using the allosteric activator chloride. It is shown that the low affinity of the psychrophilic a-amylase for chloride is entropically driven. The high enthalpic and entropic contributions of activator binding suggest large structural fluctuations between the free and the bound states of the cold-active enzyme. The kinetic and thermodynamic data for the psychrophilic a-amylase indicate that the strictly conserved side-chains involved in substrate binding and catalysis possess an improved mobility, responsible for activity in the cold, and resulting from the disappearance of stabilizing interactions far from the active site. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailKinetics and mechanism of the exchange reactions catalysed by the oxoglutarate translocator of rat-heart mitochondria.
Sluse, Francis ULg; Goffard, George; lièbecq, Claude

in European Journal of Biochemistry (1973), 32

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See detailKinetics and mechanism of the exchange reactions catalysed by the oxoglutarate translocator of rat-heart mitochondria.
Sluse, Francis ULg; Lièbecq, Claude

in Biochimie (1973), 55(6), 747-754

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See detailKinetics and Thermodynamics of Glucose Isomerase Crystallization
Sleutel, Mike; Willaert, Ronnie; Gillespie, Christopher et al

in Crystal Growth & Design (2009), 9

A quantitative study using laser confocal microscopy combined with differential interference microscopy on the kinetics and thermodynamics of the crystallization of glucose isomerase is presented ... [more ▼]

A quantitative study using laser confocal microscopy combined with differential interference microscopy on the kinetics and thermodynamics of the crystallization of glucose isomerase is presented. Fundamental crystallization parameters are determined from the kinetics of step advancement and rates of two-dimensional (2D) nucleation. The ruling mass transfer pathway and accompanying activation barriers are discussed. In brief, the solubility exhibits normal temperature dependence and the crystallization enthalpy is the thermodynamic driving force. The diminishing entropic cost for higher PEG concentrations is attributed to water structuring and a decrease in water activity. The prominent step generation mechanism is homogeneous 2D nucleation for high supersaturations. At low driving forces 2D nucleation occurs on anomalously hyperactive sites and the step edge free energies for homogeneous and heterogeneous nucleation are determined. The number of nucleation centers for both mechanisms are estimated and from the density of nucleation centers we obtain for the activation barrier of adsorption ∼3.8 kJ mol-1. No step-step interaction is observed for interstep distances >70 nm. Theoretical fits of step velocity data suggest surface diffusion makes a non-negligible contribution to surface kinetics. From the temperature dependence of the step kinetic coefficient the activation barrier for crystallization was determined to be <22.4 kJ mol-1. [less ▲]

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See detailKinetics modelling of convective heat drying of wastewater treatment sludge
Léonard, Angélique ULg; Salmon, Thierry ULg; Janssens et al

in Flamand, G. (Ed.) Récents progrés en génie des procédés vol.13 n.68 : separations. ECCE 2 conference Montpellier 5/7.10.99 (1999, October)

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See detailKinetics of 2-oxoglutarate transport in heart mitochondria
Sluse, Francis ULg; Ranson, Monique; Lièbecq, C.

in Journal de Physiologie (1971), 63

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See detailThe kinetics of a microbial population
Gillet, Marie-Claire ULg; Remacle, Jean

in Loutit, M. W.; Miles, J. A. R. (Eds.) Microbila Ecology (1978)

The study is an attempt to show the relationship between environmental variables such as pH and temperature and the control of some microbial growth characteristics. Two models were used in order to ... [more ▼]

The study is an attempt to show the relationship between environmental variables such as pH and temperature and the control of some microbial growth characteristics. Two models were used in order to calculate the death rate in continuous cultures. [less ▲]

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See detailKinetics of a specific antibody response after successful treatment in naturally Psoroptes ovis infested cattle
Lonneux, J. F.; Bossaert, K.; Leclipteux, T. et al

Conference (1995)

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See detailKinetics of C-Reactive Protein (CRP) following maintenance infliximab treatment in Crohn's disease identifies profiles of patients with better outcome
Jürgens, M.; Mahachie John, Jestinah ULg; Cleynen, I. et al

in Gastroenterology (2010), 138(5 (Suppl I)), -686

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See detailKinetics of circulating antigens in pigs experimentally infected with Taenia solium eggs
Nguekam, A.; Zoli, A. P.; Vondou, L. et al

in Veterinary Parasitology (2003), 111(4), 323-332

Three groups of four piglets were experimentally infected with different doses (10(3), 10(4) and 10(5)) of Taenia solium eggs whereas a fourth group of two pigs received gravid proglottids. At autopsy 6 ... [more ▼]

Three groups of four piglets were experimentally infected with different doses (10(3), 10(4) and 10(5)) of Taenia solium eggs whereas a fourth group of two pigs received gravid proglottids. At autopsy 6 months post infection, the two latter pigs were heavily infected with more than 3000 living cysts per kg of muscle. Ten of the 12 other pigs harboured light infections, i.e. between 2 and 107 cysticerci, 42.4% of which were degenerated. The two remaining pigs had no detectable cysts at post mortem examination. Circulating antigens (CA) were detected in the sera of all pigs harbouring living cysticerci using a monoclonal antibody based ELISA. CA were first detected between 2 and 6 weeks post infection and remained present generally throughout the entire observation period even in pigs carrying only five to eight living cysts, although strong fluctuations of the level of CA were observed in some pigs. In animals without living cysts at post mortem CA were only detected for a short period and disappeared presumably when the cysticerci became degenerated. The minimum number of living cysts, which could be detected using this ELISA, was 1. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailKinetics of concomitant transfer and hydrolysis reactions catalysed by the exocellular DD-carboxypeptidase-transpeptidase of streptomyces R61
Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg; Perkins, Harnold R. et al

in Biochemical Journal (1973), 135(3), 483-492

When Ac(2)-l-Lys-d-Ala-d-Ala and either meso-diaminopimelic acid or Gly-l-Ala are exposed to the exocellular dd-carboxypeptidase-transpeptidase of Streptomyces R61, transpeptidation reactions yielding Ac ... [more ▼]

When Ac(2)-l-Lys-d-Ala-d-Ala and either meso-diaminopimelic acid or Gly-l-Ala are exposed to the exocellular dd-carboxypeptidase-transpeptidase of Streptomyces R61, transpeptidation reactions yielding Ac(2)-l-Lys-d-Ala-(d)-meso- diaminopimelic acid and Ac(2)-l-Lys-d-Ala-Gly-l-Ala occur concomitantly with the hydrolysis of the tripeptide into Ac(2)-l-Lys-d-Ala. The proportion of the enzyme activity which can be channelled in the transpeptidation and the hydrolysis pathways depends upon the pH and the polarity of the environment. Transpeptidation is favoured both by increasing the pH and by decreasing the water content of the reaction mixtures. Kinetics suggest that the reactions proceed through an ordered mechanism in which the acceptor molecule (meso-diaminopimelic acid or Gly-l-Ala) binds first to the enzyme. Both acceptors behave as non-competitive inhibitors of the hydrolysis pathway. Transpeptidation is inhibited by high concentrations of Gly-l-Ala but not by high concentrations of meso-diaminopimelic acid. The occurrence on the enzyme of an additional inhibitory binding site for Gly-l-Ala is suggested. [less ▲]

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See detailKinetics of engraftment following allogeneic hematopoietic cell transplantation with reduced-intensity or nonmyeloablative conditioning.
Baron, Frédéric ULg; Little, Marie-Terese; Storb, Rainer

in Blood Reviews (2005), 19(3), 153-64

Nonmyeloablative or reduced-intensity conditioning regimens have been used to condition elderly or ill patients with hematological malignancies for allogeneic hematopoietic cell transplantation (HCT ... [more ▼]

Nonmyeloablative or reduced-intensity conditioning regimens have been used to condition elderly or ill patients with hematological malignancies for allogeneic hematopoietic cell transplantation (HCT). Initial mixed donor/host chimerism (i.e. the coexistence of hematopoietic cells of host and donor origin) has been observed in most patients after such transplants. Here, we describe both factors affecting engraftment kinetics in patients given a nonmyeloablative or a reduced-intensity conditioning, and associations between peripheral blood cell subset chimerism levels and HCT outcomes. [less ▲]

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