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See detailIn vitro micropropagation of Jatropha curcas L. from bud aggregates
Medza Mve, Samson Daudet ULg; Mergeai, Guy ULg; Druart, Phillipe et al

in Journal of Technology Innovations in Renewable Energy (2013), 2

Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l-1 citric acid, 12.2 ... [more ▼]

Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l-1 citric acid, 12.2 mg.l-1 adenine sulfate, 15 mg.l-1 L-arginine, 2.46 µM IBA (indole-3-butyric acid), 30 g.l-1 sucrose and 7 g.l-1 of agar, and enriched with different balances of BA (benzyladenine) and L glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 µM BA and 25 mg.l 1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in a MS medium supplemented with 2.21 µM BA, 5.70 µM IAA (indole-3-acetic acid) and 15 mg.l-1 L arginine. These shoots were isolated and then rooted in MS containing 2.46 µM of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable. [less ▲]

Detailed reference viewed: 66 (9 ULg)
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See detailIn vitro model to study the biological properties of humic fractions from landfill leachate and leonardite during root elongation of Alnus glutinosa L. Gaertn and Betula pendula Roth.
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

in Plant Cell, Tissue & Organ Culture (2015), 122(3), 739-749

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Our study aims to determine the main biological properties of HS ... [more ▼]

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Our study aims to determine the main biological properties of HS comparing landfill leachate (LHS) source to a stable formulation extracted from leonardite (HHS), and using an in vitro system of root development from shoot and leaf explants of silver birch (Betula pendula Roth) and black alder (Alnus glutinosa L. Gaertn). Results showed that both explants of both species rooted closely to 100% when cultivated in absence of HS. The incorporation of HS or their fractions into the culture medium affect root growth, mainly lateral roots formation and primary root length. Applied at low concentration (10 ppm) HS stimulated especially primary root growth. But at high concentration (100 ppm), LHS inhibited root formation of alder, while birch was more tolerant. The application of 100 ppm of HHS, did not affect alder root growth but increased root growth in birch. Humic acids fractions (HA) were favorable and improved root growth while, fulvic acids (FA) and other molecules (OM) decreased significantly root growth, especially those extracted LHS. The root inhibition expressed at high LHS concentration may be due to the presence of different toxic molecules and root growth inhibitors in OM and FA fractions and that some of them remained in the OM fraction from leonardite. [less ▲]

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See detailIn vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

Poster (2013, November 06)

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers ... [more ▼]

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers like baby bottle … BPA can migrate into food in contact with polycarbonate. There is a worldwide concern about BPA because several studies have shown endocrine disruptor potency of BPA causing possible adverse health effects. In January 2011, the European Commission decided to ban the use of polycarbonate to manufacture baby feeding bottles. In a recent opinion, the Superior Health Council’s issued its concern regarding the currently use of alternatives to polycarbonate in these materials. This work is part of the ALTPOLYCARB project which aims to study the migration products of alternative to polycarbonate and their endocrine disruptor activities. The first part was to have an overview of the different polymers replacing polycarbonate, that are used on the Belgian market, it resulted in the conclusion that polymers used for the manufacture of baby bottles are mainly polypropylene, polyethersulfone, silicone, polyamide, polystyrene, and melamine. The second part of this work will be to evaluate the endocrine disruptor activity(ies) of global migration residues obtained from different kinds of baby bottles. This (these) activity(ies) will be explored using cell based transactivational assays also named “reporter gene assays. The MCF7 recombinant cells used here are genitically modified cells containing the firefly luciferase gene, as a reporter gene, and a DNA responsive element specific to the human oestrogen receptor. The biological activity of a chemical compound is monitored by the measurement of light emitted by the cells exposed to it (after addition of luciferin, the substrate of luciferase). In a preliminary step, we first screened pure substances which were shown to migrate from plastic baby bottle, in a recent study performed by Simoneau & al, 2012 . Human estrogen receptor agonistic and antagonistic activities of 25 pure compounds were measured using MCF7-ER cells (genetically modified MCF7 cells). After the first screening, some substances clearly show an activity such as BPA, Benzophenone, 2-Propenoic acid-2-ethylhexyl ester, Benzaldehyde-4-methylthio, Butylated hydroxytoluene and Dodecanoic acid, methyl ester whereas others ask an in-depth analysis to confirm their activity. For active substances only the study will be continued and a full dose-response curve will be performed in order to assess quantitatively the activity [less ▲]

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See detailIn vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

Poster (2014, May 15)

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, which are widely used in a variety of common products including digital ... [more ▼]

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, which are widely used in a variety of common products including digital media (e.g., CDs, DVDs), electrical and electronic equipment, automobiles, sports safety equipment, reusable food and drink containers , as well as baby bottle. During the last decades, in several studies, the migration of BPA is documented to be a well-known source of food contamination. The measurements of BPA in human fluids and tissues highlighted that its presence in food constitutes the primary route of human exposure. Some studies showed that BPA, which could disrupt normal endocrine function by mimicking estrogen hormones,, may be associated to several health problems and diseases. Recently, the European food safety authority conducted a risk assessment on BPA and concluded that though studies related to potential health hazards associated with BPA, are suggesting a potential negative effect on human body, but results are still uncertain. Following that screening assessment, the European Union took a series of measures, including a ban for the manufacture, import and sale of PC baby bottles to reduce BPA exposure of infants. Plastic alternatives to polycarbonate have massively appeared on Belgium market. Although there are several studies on BPA migration from polycarbonate into foodstuff under various conditions, there is a small amount of information about consequences on human health of the chemicals migrating from PC alternatives, including bottles commonly labelled “free BPA”. In a recent opinion (No. 8697, 11.03.2010), the Belgium Superior Health Council's issued its concern regarding the currently used alternatives to PC. Furthermore, they asked to investigate the possible risks associated with the use of these alternatives. To know if these alternatives are safe, the activity on several receptors (estrogen (ER), androgen (AR), progesterone (PR) and glucocorticoïd receptor (GR)) of chemicals migrating from PC alternatives, identified by Simoneau & al, 2012 , were evaluated using reporter gene assays. Agonistic and antagonistic activities of 38 pure compounds were measured. After the first screening, some substances clearly showed an activity on each receptor, such as BPA, 2.4- dimethyl benzaldehyde (C4), Bisphenol S (C49), while other subtances reacted on three, two or one receptor. Only 5 substances showed no activity. For active substances only, the study will be continued and a full dose-response curve will be performed in order to assess quantitatively the activity. [less ▲]

Detailed reference viewed: 25 (2 ULg)
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See detailIn vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

Poster (2013, December 06)

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers ... [more ▼]

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers like baby bottle, cups, etc. BPA can migrate into food in contact with polycarbonate. There is a worldwide concern about BPA because several studies have shown endocrine disruptor potency of BPA causing possible adverse health effects. In January 2011, the European Commission decided to ban the use of polycarbonate to manufacture baby feeding bottles. In a recent opinion, the Superior Health Council’s issued its concern regarding the currently use of alternatives to polycarbonate in these materials. This work is part of the ALTPOLYCARB project, which aims to study the migration products from non polycarbonate plastic material, and their endocrine disruptor activities. The endocrine disruptor activity(ies) of global migration residues obtained from different kinds of baby bottles will be explored using cell based transactivational assays also named “reporter gene assays”. The MCF7 recombinant cells used here (named MCF7-ER cells) are genetically modified cells containing the firefly luciferase gene, as a reporter gene, and a DNA responsive element specific to the human estrogen receptor. The biological activity of a chemical compound is monitored by the measurement of light emitted by the cells exposed to it (after addition of luciferin, the substrate of luciferase). In a preliminary step, we first screened pure substances, which were shown to migrate from plastic baby bottles, in a recent study performed by Simoneau & al, 2012 . Human estrogen receptor agonistic and antagonistic activities of 25 pure compounds were measured using MCF7-ER cells. After the first screening, some substances clearly show an activity such as BPA, benzophenone, 2-propenoic acid-2-ethylhexyl ester, benzaldehyde-4-methylthio, butylated hydroxytoluene and dodecanoic acid methyl ester, whereas others ask an in-depth analysis to confirm their activity. For active substances only, the study will be continued and a full dose-response curve will be performed in order to assess quantitatively the activity. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailIn vitro modelisation of prions neuroinvasion mediated by dendritic cells
Dorban, G.; Defaweux, Valérie ULg; Heinen, Ernst ULg et al

Poster (2008, October)

Detailed reference viewed: 15 (10 ULg)
Peer Reviewed
See detailIn vitro models for the study of cartilage damage and repair
Henrotin, Yves ULg; Labasse, A; Zheng, SX et al

in Rheumatology in Europe (1998), 27(S2), 7

Detailed reference viewed: 10 (3 ULg)
See detailIn vitro models for the study of cartilage damage and repair
Henrotin, Yves ULg; Reginster, Jean-Yves ULg

in Reginster, Jean-Yves; Pelletier, J-P; Martel-Pelletier, J (Eds.) et al Osteoarthritis: Clinical and experimental aspects (1999)

Detailed reference viewed: 9 (2 ULg)
See detailIn vitro models of non persistent and persistent infection of human and murine neuroblastoma cell lines by the varicella zoster virus
Schlabertz, Tania; Sadzot-Delvaux, Catherine ULg; Piette, Jacques ULg et al

in Archives Internationales de Physiologie et de Biochimie (1997)

Detailed reference viewed: 38 (4 ULg)
Peer Reviewed
See detailIn vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCL.
Van Heusden, Alain ULg; Nusgens, Betty ULg; Goffinet, Gerhard ULg et al

in Journal of Periodontal Research (1998), 33(6), 377-85

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce ... [more ▼]

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated amino-acids. Increasing concentrations of minocycline (10, 50, 100 micrograms/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100 micrograms/ml which statistically significantly (p < 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 micrograms/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 micrograms/ml of minocycline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10 micrograms/ml minocycline-HCl solution increased significantly (p < 0.005) cell migration towards a gradient of fetal calf serum. The presence of 10 micrograms/ml of minocycline in contact with the keratinocytes in the upper compartment of the migration chambers also produced a significant (p < 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemoattractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyond 50 micrograms/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of a junctional epithelium. [less ▲]

Detailed reference viewed: 46 (14 ULg)
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See detailIn vitro modulation of human gingival keratinocyte migration by minocycline-HCl
Van Heusden, Alain ULg; Nusgens, Betty ULg; Lapière, Charles et al

in Journal of Dental Research (1998), 77

Detailed reference viewed: 18 (3 ULg)
Peer Reviewed
See detailIn vitro modulation of keratinocyte attachment by minocycline H-Cl
Van Heusden, Alain ULg; Rompen, Eric ULg; Lebfevre, P. et al

in Journal of Dental Research (1995), 74

Detailed reference viewed: 17 (3 ULg)
Peer Reviewed
See detailIn vitro modulation of keratinocyte attachment by minocycline HCl.
Van Heusden, Alain ULg; Rompen, Eric ULg; Lebfevre, P. et al

Poster (1994)

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailIn vitro modulation of keratinocyte attachment to dentin by minocycline
Van Heusden, Alain ULg; Rompen, Eric ULg; Lapière, Charles

in Journal of Dental Research (1997), 76

Detailed reference viewed: 16 (3 ULg)
Peer Reviewed
See detailIn vitro modulation of radiosenitizing effect of FMdC. The importance of simultaneous alteration of the novo and salvage pathways to deoxyribonucleosides.
COUCKE, Philippe ULg; Cottin, E; Ciernick, I-F et al

in International Journal of Radiation, Oncology, Biology, Physics (2000), 46(3),

Detailed reference viewed: 5 (0 ULg)
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See detailIn vitro multiplication of an industrial fiber plant: kenaf (Hibiscus cannabinus L.)
Arbaoui, Sarra; Campanella, Bruno ULg; Paul, Roger ULg et al

Poster (2012, April 23)

Detailed reference viewed: 151 (8 ULg)
See detailIn vitro neuroblastoma cell infection by varicella-zoster virus
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Piette, Jacques ULg et al

Poster (1988)

Detailed reference viewed: 10 (0 ULg)