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See detailIsolation of pregnancy-associated glycoproteins of the American bison (Bison bison) at first half of pregnancy
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in General and Comparative Endocrinology (2008), 155(1), 164-175

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins ... [more ▼]

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins were purified from foetal cotyledonary tissues (CT) and liquid cotyledonary-carrying proteins (LCP) leaking from damaged cells. Our protocols successfully indicated the usefulness of AmbPAG protein identification, especially from LCP fraction. The AmbPAGs were extracted, precipitated and eluted during DEAE cellulose chromatography. The richest protein fractions were further chromatographed on VVA (Vicia villosa agglutinin affinity column), then characterised by mono- and bi-dimensional electrophoresis, Western blot and N-terminal amino acid (aa) sequence. After being transferred to PVDF membranes, three selected VVA-purified AmbPAG isoforms differing in molecular masses and isoelectric points (Ip 4-4.6) were selected for sequencing. One identified N-terminal 25 aa sequence of AmbPAG72 kDa CT form was identified as completely new (RGSNI_TSLPLQNVIDLFYVGNITIG). Two other AmbPAG proteins purified from different sources (74 kDa CT and 76 kDa LCP forms; RGSNLTIHPLRNIRDIFYVGNITIG) were identical or corresponded to N-terminus of various bovine PAGs (boPAG). The two AmbPAGs (74 kDa CT and 76 kDa LCP) revealed identical micro-sequence to boPAG7; and were similar mainly to bovine PAG4, -6, -15 and -17 precursors that were identified by full-length sequencing derived from cDNA cloning. The novel sequence of the AmbPAG (72 kDa CT) was related to some boPAG and various other ruminant PAG precursors (caprine and ovine). All three identified AmbPAG sequences were also relatively similar to mature forms of purified native boPAG56-75kDa proteins. This is the first report indicating aa sequences of native AmbPAG proteins purified from placenta (CT and LCP) of bison species. The N-terminal sequences of the AmbPAGs have been deposited in the EMBL-EBI database (UniProtKB; Accession Nos.: P84916, P84917 and P84918). (C) 2007 Elsevier Inc. All rights reserved. [less ▲]

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See detailIsolation of shiga toxin-producing Escherichia coli from a South American camelid (Lama guanicoe) with diarrhea.
Mercado, E. C.; Rodriguez, Sabrina ULg; Elizondo, A. M. et al

in Journal of Clinical Microbiology (2004), 42(10), 4809-11

Shiga toxin-producing Escherichia coli belonging to serotype O26:H11 was isolated from a 2-month-old guanaco with severe watery diarrhea. E. coli colonies carried the stx1 and eae genes, showed localized ... [more ▼]

Shiga toxin-producing Escherichia coli belonging to serotype O26:H11 was isolated from a 2-month-old guanaco with severe watery diarrhea. E. coli colonies carried the stx1 and eae genes, showed localized adherence to HEp-2 cells, and produced enterohemolysin. A serological response to lipopolysaccharide O26 was observed at the onset of diarrhea. [less ▲]

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See detailISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS
ELLING, Lothar; GULDENBERG, Birgit; GROTHUS, Marita et al

in Biotechnology & Applied Biochemistry (1995), 21(Part 1), 29-37

The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the ... [more ▼]

The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the 9 kg scale of rice (Oryza sativa) grains was achieved in five steps with 11.3% yield and 192-fold purification. The final sucrose synthase preparation was free of nucleotide di- and monophosphatases, but did contain 0.05% of both invertase and UDP-glucose-hydrolysing activity. It can be efficiently utilized for the synthesis of activated sugars. Its application for the synthesis of saccharides is possible taking into account that 15% UDP-glucose is hydrolysed within 15 h incubation time [less ▲]

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See detailISOLATION OF THE ANTIMICROBIAL CYCLIC PEPTIDE SUBTILOSIN A FROM A GUT-ASSOCIATED BACILLUS SUBTILIS STRAIN
Schyns, Ghislain; Serra, Claudia; Henriques, Adriano et al

in American Journal of Biochemistry & Biotechnology (2013), 9(3),

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See detailIsolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase
Nguyen-Distèche, Martine ULg; Leyh-Bouille, Mélina; Ghuysen, Jean-Marie ULg

in Biochemical Journal (1982), 207(1), 109-115

The membrane-bound, 26 000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in the form of an effective, penicillin-sensitive D-alanyl-D-alanine-cleaving peptidase exhibiting high ... [more ▼]

The membrane-bound, 26 000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in the form of an effective, penicillin-sensitive D-alanyl-D-alanine-cleaving peptidase exhibiting high transpeptidase activity (greater than 95%) and very low carboxy-peptidase activity (less than 5%). The penicillin-binding protein/transpeptidase can be extracted directly from the mycelium with N-cetyl-NNN-trimethylammonium bromide (Cetavlon) and subsequently obtained at 90% purity and with an 8000-fold specific enrichment (when compared with the activity of the isolated membranes) by a two-step procedure involving Sephadex filtration and affinity chromatography on ampicillin-linked CH Sepharose 4B in the presence of detergent. At saturating concentrations of the co-substrates diacetyl-L-Lys-D-Ala-D-Ala and Gly-Gly, the catalytic-centre activity is about 0.3 s-1. [less ▲]

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See detailIsolation of the missing 5-end of the encoding region of the bovine leukemia virus cell receptor gene.
Ban, J.; Truong, A. T.; Horion, B. et al

in Archives of Virology (1994), 138(3-4),

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See detailIsolation of the pregnancy-associated glycoprotein 1 (PAG-1) from zebu (Bos indicus) placenta.
Melo de Sousa, Noelita ULg; Remy, B.; El Amiri, B. et al

in Proceedings of the 16th Scientific Meeting of the European Embryo Transfer Association. (2000)

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See detailL'isolation thermique de la toiture inclinée - Guide pratique pour les architectes
Simon, Francy; Hauglustaine, Jean-Marie ULg

Book (1999)

In 1996, for primarily environmental motivations, the Walloon Government reinforced the requirements as regards thermo isolation of the buildings of the sectors residential and tertiary. For the new ... [more ▼]

In 1996, for primarily environmental motivations, the Walloon Government reinforced the requirements as regards thermo isolation of the buildings of the sectors residential and tertiary. For the new housing, the level of total thermo isolation necessary K55 replaced the K70 level, while a K65 level was fixed for the lately built offices and the schools. In alternative, the energy needs Be 450 replaced the needs Be 500 required previously for the new housings. The intervention of the originators is essential because it returns to them to integrate the performances to be reached, as of the initial stages of the process of design. These performances should not however slow down their creativity; on the contrary, it is a question of building buildings combining beauty, comfort, durability and economy. Conscious of the importance of information to be brought to the professionals on the one hand and to the public on the other hand, the Walloon region has set up, for a few years, a multiform device of information and formation, relaying the research undertaken by the Scientific and Technical Center of Construction and the Universities. A first handy guide intended for the architects explored the methodology of design of the thermo isolation of the hollow wall. Its authors, Francy Simon, Professor at the Catholic of Leuwen, and Jean-Marie Hauglustaine, ir University. of research - doctorand at the University of Liege, continue this approach for the thermo isolation of the inclined roof, proposing a coherent step, draft with the project. In this guide, the architect will find a step conceptual integrated allowing him to direct and adapt his choices, by the recourse in particular to tables presenting the current systems of inclined roofs, their performances, as well as a predimensioning thicknesses of insulator and type of complementary vapor barrier possibly necessary. Another guide intended for roofers and for carpenters is currently diffused by Funds of Professional training of Construction, while the private individuals can find booklets and councils which go in the same direction, near the Counters of Energy. These achievements are integrated in the policy which the Rational Use of Energy (RUE) in the buildings has carried out the Walloon region for several years in order to support. Belgium indeed engaged, in Rio and Kyoto, to decrease the consumption of energy and thus the CO2 emissions, in order to reduce the greenhouse effect. Let us guarantee that we will reach that point thanks to the efforts of all. [less ▲]

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See detailL'isolation thermique de la toiture plate - Guide pratique pour les architectes
Simon, Francy; Hauglustaine, Jean-Marie ULg; Liesse, Sophie et al

Book (2003)

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See detailL'isolation thermique des façades à structure bois - Guide pratique pour les architectes
Simon, Francy; Hauglustaine, Jean-Marie ULg; Baltus, Catherine et al

Book (2003)

Detailed reference viewed: 86 (7 ULg)
See detailL'isolation thermique des façades verticales - Guide pratique pour les architectes
Hauglustaine, Jean-Marie ULg; Simon, Francy; Baltus, Catherine et al

Book (2006)

For a few years, the concerns of the scientific world as for the climatic balance of the Earth have found echo in the civil society and the political world. The population itself starts to become aware of ... [more ▼]

For a few years, the concerns of the scientific world as for the climatic balance of the Earth have found echo in the civil society and the political world. The population itself starts to become aware of the situation. But it is not the only stake with which we are confronted. The security of supply, in particular, must be anticipated as of today: the consumption of energy increases continuously (it should double from here 2030), whereas reserves - out of coal, oil, natural gas and uranium - become exhausted ineluctably. Vis-a-vis these real challenges, the control of the request constitutes however also a paramount element: to take again a sentence become famous, best energy remains that which one does not consume! In this last field, the energy performance of the buildings is a factor impossible to circumvent. The more so as the European directive of December 16th, 2002 on the energy performance of the buildings (which must be transposed for January 2006) will involve a small revolution in the sector of construction and, in the long term, in the restoration of the buildings. Even if this directive invites us to reason in terms of total performance, fascinating in consideration the whole of the energy characteristics of the building (envelope, equipment of heating and water provision heat, installations of air-conditioning, ventilation, site and orientation of the buildings, solar systems passive and solar protection, natural ventilation, interior climatic quality,…), the envelope of the building remains certainly the first element to be considered. The Walloon regulation has forced for several years the new buildings to respect a level of total thermo isolation minimum for the envelope. It goes without saying that the tendency in the future, in particular have regard to the objectives laid down at the European level, will be to reinforce the current requirements on the matter. This new work which comes to enrich the collection by “Handy guides for the Architects” while exploring, under the angle of energy, the methodology of design of the frontages of a building, fact continuation more particularly with the guides devoted to the hollow walls and the frontages with framework wood. Energy is only one of the many parameters with which an architect must juggle and it is perhaps not your priority today. However, I am persuaded that our practices should be changed on the matter and that the architect is “angular stone” of the challenges which the control constitutes of energy in the building. This one is economic imperative, social and environmental. [less ▲]

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See detailL'isolation thermique des murs creux - Guide pratique pour les architectes
Simon, Francy; Hauglustaine, Jean-Marie ULg

Book (1996)

The Walloon regulation imposes on the new buildings a minimum level of insulation. The level standard K 70 is now reinforced while passing to K 55, while the regulation extends to other types of buildings ... [more ▼]

The Walloon regulation imposes on the new buildings a minimum level of insulation. The level standard K 70 is now reinforced while passing to K 55, while the regulation extends to other types of buildings. The concern of insulating well should not however attach the creativity of the architect who aims at carrying out a beautiful frame, livable, durable and of a minimum cost. This is why this work proposes a whole of devices making it possible to reconcile these goals. The design and the drafting of this guide were entrusted to professor Francy SIMON of the Catholic University of Leuwen and to Jean-Marie HAUGLUSTAINE, attending the University of Liege; their work was followed by a reading panel where the Order of the Architects and the French-speaking Federation of the companies of architects of Belgium were represented. Moreover, these two institutions supported the impression and the diffusion of this document and I thank them for this collaboration. But the architect who wants to progress in this way will undoubtedly meet a certain reserve of the trade associations de with regard to new techniques and more complex devices. This is why, also with the assistance of the Walloon region, another guide for the insulation of the hollow walls, intended to the masons and for the carpenters, is diffused by the Funds of professional training of construction. Thus, by these initiatives, the Area prolongs and amplifies the information and research task carried out since many years by the Scientific and Technical Center of Construction. This multiform device to improve the current practice of construction doubles of a description of the achievements of particularly innovating architects, by the contest PHOTOGRAVURE, like an research effort, whose most visible element is the house PLEIAD, with Leuwen-the-New. To convince the companies to accept his requirements for quality, the architect is thus not alone. He is not it either in his effort of conviction with regard to the building owners. The private individuals find, in the Counters of the Energy of the Walloon region, the publications and the councils which go in the same direction. All this relates to the residential or tertiary building. Industry and the public corporations are the subject of the same policy of rational use of energy. Thus, the Walloon Government shows its will to reduce our dependence and our puncture on resources to be divided with the people under development but also to reduce the harmful effects for natural environment and health. It is there a commitment which Belgium undertook in Rio and to which the Walloon region subscribed. Together, we will reduce the consumption of energy and thus the CO2 emissions. We are committed there; we all must work to achieve this goal. [less ▲]

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See detailIsolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the edible dormouse, Glis glis
Huerner, H.; Martin, Jacques ULg; Ribas, A. et al

in Molecular Ecology Resources (2009), 9(3), 885-887

We isolated and characterized 10 dinucleotide microsatellite loci in the edible dormouse, Glis glis (Linnaeus). Four multiplex panels were developed. Loci were amplified in samples from two geographically ... [more ▼]

We isolated and characterized 10 dinucleotide microsatellite loci in the edible dormouse, Glis glis (Linnaeus). Four multiplex panels were developed. Loci were amplified in samples from two geographically distant populations (Torgny in Belgium and Montseny in Spain). All loci were polymorphic in Spain but four were monomorphic in Belgium. Individuals from Belgium and Spain exhibited an average allelic diversity of 1.9 and 3.3 and an observed heterozygosity ranging from 0.08 to 0.47 and from 0.04 to 0.72, respectively. [less ▲]

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See detailIsolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli
Latinne, Alice ULg; Waengsothorn, Surachit; Risterucci, Ange Marie et al

in Conservation Genetics Resources (2011), 3

Leopoldamys neilli is a threatened murine rodent species endemic to limestone karsts of Thailand. Twelve microsatellite loci were identified using the method of microsatellite-enriched libraries ... [more ▼]

Leopoldamys neilli is a threatened murine rodent species endemic to limestone karsts of Thailand. Twelve microsatellite loci were identified using the method of microsatellite-enriched libraries. Polymorphism was assessed in samples (N=62) from four geographically distinct populations in Thailand. Number of alleles per locus ranged from 9 to 15 (average 11.6). Observed and expected heterozygosities varied from 0.28 to 1.0 and from 0.44 to 0.91, respectively. There was no evidence for linkage disequilibrium, however, four loci showed evidence of departure from Hardy–Weinberg equilibrium in one population. Presence of null alleles was not detected in all the 12 loci. These first microsatellites primers developed for L. neilli will provide information on the fine-scale genetic structure of this threatened species and will help in the development of future conservation policies. [less ▲]

Detailed reference viewed: 34 (14 ULg)