Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (2006), 70(4), 291-296

The aim of this work was to measure the myeloperoxidase (MPO) concentration in bronchoalveolar lavage (BAL) fluid collected from horses with recurrent airway obstruction (RAO), both in crisis and in ... [more ▼]

The aim of this work was to measure the myeloperoxidase (MPO) concentration in bronchoalveolar lavage (BAL) fluid collected from horses with recurrent airway obstruction (RAO), both in crisis and in remission, as well as from healthy horses. Seven horses with RAO were exposed to moldy hay until the maximum change in pleural pressure was greater than 1.5 kPa. At that point, BAL was performed, and the total cell counts and percentages in the fluid were immediately determined. To measure the MPO concentration in BAL-fluid supernatant, we used a specific enzyme-linked immunosorbent assay with polyclonal antibodies against equine MPO. The tests were repeated on the horses with RAO after they had spent 2 mo on pasture. Six healthy horses serving as controls underwent the same tests. The absolute and relative neutrophil counts and the MPO concentration in the BAL fluid were significantly greater in the horses with an RAO crisis than in the control horses. After 2 mo on pasture, the horses that had been in RAO crisis were clinically normal, and their neutrophil counts and MPO levels in BAL fluid had significantly decreased; during remission their neutrophil counts were not significantly different from those in the healthy horses, but their MPO concentration remained significantly higher. This study showed that determining the MPO concentration in a horse's BAL fluid is technically possible and that during remission from RAO the concentration remains higher than normal. Thus, MPO may be a marker of neutrophil presence and activation in the lower airways. [less ▲]

Myeloperoxydase concentration in bronchoalveolar lavage from healthy and heavy horses

in Proceedings : 22nd Symposium of the Veterinary and Comparative Respiratory Society (2004)

Myocardial contractile reserve during exercise predicts left ventricular reserve remodelling after cardiac resynchromization therapy

in European Journal of Echocardiography (2009), 10(5), 663-8

Myocardial expression of Estrogen Receptors in patients with congenital cardiac defect is associated with peri-operative myocardial protection

Poster (2012)

Myocardial remodelling in children with atrial septal defect and significant left-to-right shunt is characterized by signals inducing hypertrophy, angiogenesis, fibrosis and inhibition of apoptosis

Poster (2012)

Myocellular Enzyme Leakage, Polymorphonuclear Neutrophil Activation and Delayed Onset Muscle Soreness Induced by Isokinetic Eccentric Exercise

in Archives of Physiology & Biochemistry (1996), 104(3), 322-9

To address the question of whether delayed onset muscular soreness (DOMS) following intense eccentric muscle contraction could be due to increased production of the arachidonic acid derived product ... [more ▼]

To address the question of whether delayed onset muscular soreness (DOMS) following intense eccentric muscle contraction could be due to increased production of the arachidonic acid derived product prostaglandin E2 (PGE2). 10 healthy male subjects were submitted to eccentric and concentric isokinetic exercises on a Kin Trex device at 60 degrees/s angular velocity. Exercise consisted of 8 stages of 5 maximal contractions of the knee extensor and flexor muscle groups of both legs separated by 1 min rest phases. There was an interval of at least 30 days between eccentric and concentric testing, and the order of the two exercise sessions was randomly assigned. The subjective presence and intensity of DOMS was evaluated using a visual analogue scale, immediately, following 24 h and 48 h after each test. Five blood samples were drawn from an antecubital vein: at rest before exercise, immediately after, after 30 min recovery, 24 h and 48 h after the tests. The magnitude of the acute inflammatory response to exercise was assessed by measuring plasma levels of polymorphonuclear elastase ([EL]), myeloperoxidase ([MPO]) and PGE2 ([PGE2]). Using two way analysis of variance, it appeared that only eccentric exercise significantly increased [EL] and DOMS, especially of the hamstring muscles. Furthermore, a significant decrease in eccentric peak torque of this muscle group only was observed on day 2 after eccentric work (- 21%; P < 0.002). Serum activity of creatine kinase and serum concentration of myoglobin increased significantly 24 and 48 h after both exercise tests. However, these variables reached significantly higher values following eccentric contractions 48 h after exercise. Mean [PGE2] in the two exercise modes remained unchanged over time and were practically equal at each time point. On the basis of these findings, we conclude that the magnitude of polymorphonuclear (PMN) activation, muscle damage, and DOMS are greater after eccentric than after concentric muscle contractions. However, the hypothesized interplay between muscle damage, increased PGE2 production, DOMS sensations, and reduced isokinetic muscle performance was not substantiated by the present results. [less ▲]

Myofibrillar composition of some red- and white-blooded Antarctic teleosts

in Molecular Physiology (1984), 5

Myoglobin in the early detection and therapy follow-up of acute myocardial infarction : comparison with heart enzymes

Conference (1990, July 23)

Myoglobine: méthodes analytiques et interprétation clinique

Conference (1991, March 25)

Myoglobinurie atypique chez les chevaux au pré: une série de cas en Belgique

Master of advanced studies dissertation (2002)

Atypical myoglobinuria in grazing horses was described in United Kingdom and Germany between 1984 and 1996. Fourteen horses were presented at the University of Liège between November 2000 and April 2001 ... [more ▼]

Atypical myoglobinuria in grazing horses was described in United Kingdom and Germany between 1984 and 1996. Fourteen horses were presented at the University of Liège between November 2000 and April 2001, with a clinical history suggesting atypical myoglobinuria. Lesions were similar to those previously described. All horses were at rest and grazing when they developed clinical signs. Three times, the syndrome killed several horses grazing together. Horses were generally found recumbent, nearly or totally unable to stand up. They were presenting tachycardia, polypnoea and myoglobinuria, and died rapidly. When performed, blood analysis revealed severe muscular enzymatic activity rises. Histopathology revealed pulmonary congestion and oedema, and squeletal muscular fibers degeneration, mostly in respiratory and postural muscles. These observations are in accordance with cases of atypical myoglobinuria previously described in UK and Germany. The aetiology of this pathology is still unknown, despite of research attempts. An epidemiological inquiry protocol was developed in order to investigate upcoming cases within the scope of aetiologic research. Atypical myoglobinuria in grazing horses was described in United Kingdom and Germany between 1984 and 1996. Fourteen horses were presented at the University of Liège between November 2000 and April 2001, with a clinical history suggesting atypical myoglobinuria. Lesions were similar to those previously described. All horses were at rest and grazing when they developed clinical signs. Three times, the syndrome killed several horses grazing together. Horses were generally found recumbent, nearly or totally unable to stand up. They were presenting tachycardia, polypnoea and myoglobinuria, and died rapidly. When performed, blood analysis revealed severe muscular enzymatic activity rises. Histopathology revealed pulmonary congestion and oedema, and squeletal muscular fibers degeneration, mostly in respiratory and postural muscles. These observations are in accordance with cases of atypical myoglobinuria previously described in UK and Germany. The aetiology of this pathology is still unknown, despite of research attempts. An epidemiological inquiry protocol was developed in order to investigate upcoming cases within the scope of aetiologic research. [less ▲]