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See detailInteraction between antimicrobial peptides and membranes
Deleu, Magali ULg

Conference (2004)

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See detailInteraction between Antitumor Drugs and a Double-Stranded Oligonucleotide Studied by Electrospray Ionization Mass Spectrometry
Gabelica, Valérie ULg; De Pauw, Edwin ULg; Rosu, Frédéric ULg

in Journal of Mass Spectrometry [=JMS] (1999), 34(12), 1328-1337

Electrospray ionization mass spectrometry was used to investigate the complex formation between a double-stranded oligonucleotide and various antitumor drugs belonging to two categories: intercalators ... [more ▼]

Electrospray ionization mass spectrometry was used to investigate the complex formation between a double-stranded oligonucleotide and various antitumor drugs belonging to two categories: intercalators (ethidium bromide, amsacrine and ascididemin) and minor groove binders (Hoechst 33258, netropsin, distamycin A, berenil and DAPI). The goal of this study was to determine whether the relative intensities in the mass spectra reflect the relative abundances of the species in the solution phase. The full-scan mass spectra suggest non-specific binding for the intercalators and specific binding for the minor groove binders. The preferential stoichiometries adopted by each minor groove binder were determined by studying the influence of the drug concentration on the spectra. We obtained 2:1 > 1:1 for distamycin, 1:1 > 2:1 for Hoechst 33258 and DAPI and only the 1 : 1 complex for netropsin and berenil. These features reflect their known behavior in solution. The compared tandem mass spectra of the 1 : 1 complexes with Hoechst 33258 and netropsin, when correlated with published crystallographic data, suggest the possibility of inferring some structural information. The relative binding affinities of the drug for the considered duplex were deduced with two by two competition experiments, assuming that the relative intensities reflect the composition of the solution phase. The obtained affinity scale is netropsin > distamycin A > DAPI > Hoechst 33258 > berenil. These examples show some of the potential uses of mass spectrometry as a useful tool for the characterization of specific drug binding to DNA, and possibly a rapid drug screening method requiring small amounts of materials. [less ▲]

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See detailInteraction between ascites susceptibility and CO during the second half of incubation of two broiler lines. Effect on embryonic development and hatching process.
Everaert, Nadia ULg; Debonne, M.; Willemsen, H. et al

in British poultry science (2010), 51(3), 335-43

1. Because CO(2) during the second half of incubation is known to influence air cell and blood gases, and embryo development, it is postulated that post-hatch development and ascites sensitivity could ... [more ▼]

1. Because CO(2) during the second half of incubation is known to influence air cell and blood gases, and embryo development, it is postulated that post-hatch development and ascites sensitivity could also be influenced. 2. An ascites susceptible (A) and an ascites resistant (E) broiler line were incubated under standard incubation or high CO(2) conditions (up to 4%) from embryonic day (ED) 10 onwards. The embryonic development and the hatching process of these two lines were compared when incubated under standard or high CO(2) conditions from over the second half of incubation. 3. The A line, selected for high post-hatch growth rate, exhibited a higher relative embryo weight from ED10 until ED16, which was supported by a higher air cell pCO(2), lower air cell pO(2), higher corticosterone and thyroid hormones and earlier hatching time. 4. Incubation under high CO(2) increased air cell pCO(2), retarded yolk consumption, and decreased glycogen concentration in the liver at hatch. Hatchability decreased in both lines when incubated under high CO(2), due to an increased late mortality of embryos that died before IP. 5. These results suggest that the development and metabolism of CO(2)-incubated embryos differ from control incubated embryos. [less ▲]

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See detailInteraction between beta-lactam antibiotics and exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61
Frère, Jean-Marie ULg; Leyh-Bouille, Mélina; Ghuysen, Jean-Marie ULg et al

in European Journal of Biochemistry (1974), 50(1), 203-214

On the basis of steady-state kinetics, inhibition of the exocellular dd-carboxypeptidase-trans-peptidase of Streptomyces R61 by β-lactam antibiotics was competitive with regard to the donor substrate ... [more ▼]

On the basis of steady-state kinetics, inhibition of the exocellular dd-carboxypeptidase-trans-peptidase of Streptomyces R61 by β-lactam antibiotics was competitive with regard to the donor substrate. However, the complexes formed between the Streptomyces R61 enzyme and various β-lactam antibiotics were relatively stable, exhibiting half-lives of 40 to 80 min at 37°C and neutral pH. During breakdown of the complexes the protein underwent reactivation, whereas the released antibiotic molecule was chemically altered. With [14C]benzylpenicillin, the released compound was neither benzylpenicillin nor benzylpenicilloic acid. The properties of the Streptomyces R61 enzyme β-lactam antibiotic complexes were compared with those of the complexes formed between the same antibiotics and either the membrane-bound transpeptidase from Streptomyces R61 or the exocellular dd-carboxypeptidase-transpeptidase of Streptomyces R39. [less ▲]

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See detailThe interaction between Bovine Leukemia Virus and its target cell.
Burny, Arsène; Kettmann, Richard ULg; Willems, Luc ULg et al

in AIDS Research and Human Retroviruses (1992), 8

Previous results indicate that the external glycoprotein gp51 of bovine leukemia virus plays an important role in the process of cell fusion induced by bovine leukemia virus (Bruck, C., Mathot, S ... [more ▼]

Previous results indicate that the external glycoprotein gp51 of bovine leukemia virus plays an important role in the process of cell fusion induced by bovine leukemia virus (Bruck, C., Mathot, S., Portetelle, D., Berte, C., Franssen, J. D., Herion, P., and Burny, A. (1982) Virology 122, 342-352; Voneche, V., Portetelle., D., Kettmann, R., Willems, L., Limbach, K., Paoletti, E., Ruysschaert, J. M., Burny, A., and Brasseur, R. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 3810-3814) and suggest that a region encompassing residues 23 and 25 of gp51 is involved in this process (Portetelle, D., Couez, D., Bruck, C., Kettmann, R., Mammerickx, M., Van der Maaten, M., Brasseur, R., and Burny, A. (1989) Virology 169, 27-33; Mamoun, R., Morisson, M., Rebeyrotte, N., Busetta, B., Couez, D., Kettmann, R., Hospital, M., and Guillemain, B. (1990) J. Virol. 64, 4180-4188). X-ray diffraction studies performed on envelope glycoproteins of influenza virus indicate that the NH2-terminal part of the external glycoprotein lies very close to the fusion peptide. The same overall structure seems to exist in human immunodeficiency virus as suggested by site-directed mutagenesis followed by syncytia induction assays. Our theoretical studies indicate that a segment expanding between residues 19 and 27 of gp51 probably adopts an amphipathic beta-strand structure. We hypothesize that the amphipathic 19-27 structure of gp51 plays an important role in the process of membrane fusion by interacting with the fusion peptide or with another region of gp30. Mutational analysis disrupting the amphipathy of the 19-27 region strongly altered the fusogenic capacity of the gp51-gp30 complex. [less ▲]

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See detailInteraction between Class B Beta-Lactamases and Suicide Substrates of Active-Site Serine Beta-Lactamases
Prosperi-Meys, C.; Llabres, Gabriel ULg; De Seny, Dominique ULg et al

in FEBS Letters (1999), 443(2), 109-11

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders ... [more ▼]

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders of magnitude. A comparison of the kinetic parameters for the alpha and beta isomers of 6-iodopenicillanic acid shows that there is no general preference for the alpha isomer and that the efficient hydrolysis of imipenem by these enzymes must rest on other factors. [less ▲]

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See detailInteraction between dendritic cells and nerve fibres in lymphoid organs after oral scrapie exposure
Dorban, Gauthier ULg; Defaweux, Valérie ULg; Demonceau, Christine ULg et al

in Virchows Archiv (2007), 451(6), 1057-1065

In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting ... [more ▼]

In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting the nervous system. To clarify the cellular requirements for the neuro-invasion of the scrapie agent from the lymphoid organs to the central nervous system, we have studied, by confocal microscopy, the innervations within Peyer's patches, mesenteric lymph nodes and the spleen of mice in physiological conditions and after oral exposure to prion. Contacts between nerve fibres and PrPsc-associated cells, dendritic cells (DCs) and follicular dendritic cells (FDCs), were evaluated in preclinical prion-infected mice. Using a double immunolabelling strategy, we demonstrated the lack of innervation of PrPsc-accumulating cells (FDCs). Contacts between nerve fibers and PrPsc-propagating cells (DCs) were detected in T-cell zones and cell-trafficking areas. This supports, for the first time, the possible implication of dendritic cells in the prion neuroinvasion process. [less ▲]

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See detailInteraction between dietary protein content and the source of carbohydrates along the gastrointestinal tract of weaned piglets.
Pieper, Robert; Boudry, Christelle ULg; Bindelle, Jérôme ULg et al

in Archives of Animal Nutrition (2014), 68

Although fermentable carbohydrates (CHO) can reduce metabolites derived from dietary protein fermentation in the intestine of pigs, the interaction between site of fermentation and substrate availability ... [more ▼]

Although fermentable carbohydrates (CHO) can reduce metabolites derived from dietary protein fermentation in the intestine of pigs, the interaction between site of fermentation and substrate availability along the gut is still unclear. The current study aimed at determining the impact of two different sources of carbohydrates in diets with low or very high protein content on microbial metabolite profiles along the gastrointestinal tract of piglets. Thirty-six piglets (n = 6 per group) were fed diets high (26%, HP) or low (18%, LP) in dietary protein and with or without two different sources of carbohydrates (12% sugar beet pulp, SBP, or 8% lignocellulose, LNC) in a 2 × 3 factorial design. After 3 weeks, contents from stomach, jejunum, ileum, caecum, proximal and distal colon were taken and analysed for major bacterial metabolites (D-lactate, L-lactate, short chain fatty acids, ammonia, amines, phenols and indols). Results indicate considerable fermentation of CHO and protein already in the stomach. HP diets increased the formation of ammonia, amines, phenolic and indolic compounds throughout the different parts of the intestine with most pronounced effects in the distal colon. Dietary SBP inclusion in LP diets favoured the formation of cadaverine in the proximal parts of the intestine. SBP mainly increased CHO-derived metabolites such as SCFA and lactate and decreased protein-derived metabolites in the large intestine. Based on metabolite profiles, LNC was partly fermented in the distal large intestine and reduced mainly phenols, indols and cadaverine, but not ammonia. Multivariate analysis confirmed more diet-specific metabolite patterns in the stomach, whereas the CHO addition was the main determinant in the caecum and proximal colon. The protein level mainly influenced the metabolite patterns in the distal colon. The results confirm the importance of CHO source to influence the formation of metabolites derived from protein fermentation along the intestinal tract of the pig. [less ▲]

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See detailInteraction between dietary protein level and source of fermentable carbohydrates in the gastrointestinal tract of pigs
Pieper, Robert; Boudry, Christelle ULg; Vahjen, Wilfried et al

in International workshop on nutrition and intestinal microbiota host interaction in the pig : Book of abstracts (2013, October 24)

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See detailInteraction between earthworms and soil fungi: volatiles attraction
Zirbes, Lara ULg; Thonart, Philippe ULg; Wathelet, Jean-Paul ULg et al

in 9th International Symposium on Earthworm Ecology (2010)

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See detailInteraction between eustacy and block-faulting in the Carboniferous of the Visé-Maastricht area (Belgium, the Netherlands)
Poty, Edouard ULg; Delculee, Sandrine ULg

in Zeitschrift der Deutschen Gesellschaft für Geowissenschaften [=ZDGG] (2011), 162(2), 117-126

The Visé–Maastricht sedimentation area (VSA) is situated at the eastern end of the Brabant Massif and suffered block-faulting tectonics during Lower Carboniferous time. The south edge of the VSA, the area ... [more ▼]

The Visé–Maastricht sedimentation area (VSA) is situated at the eastern end of the Brabant Massif and suffered block-faulting tectonics during Lower Carboniferous time. The south edge of the VSA, the area corresponding now to the vicinity of Visé, comprised three main tectonic blocks: the Hermalle-sous-Argenteau, Souvré and Bombaye blocks. They were bounded to south by the Booze–Val-Dieu block. To the north, in the vicinity of Maastricht, blocks are not well differ- entiated and are referred to as the Maastricht block system. From the end of the Tournaisian, the latter evolved into a rap- idly subsiding graben, recording mainly debris fl ows and limestone turbidites, whereas the southern blocks remained rela- tively high. Through much of Lower Carboniferous time, they were emergent, but during high eustatic sea levels, they were fl ooded and covered by limestone deposits. The differences in the nature and the age of the deposits between blocks result from the interaction between block-faulting and eustacy. In the VSA, the Upper Devonian and Lower Tournaisian (Hastarian) deposits are similar to those known in the north part of the Namur-Dinant Basin. But from the late Tournaisian (Ivorian), the uplift of the Booze–Val-Dieu block prevented all connections with the Namur-Dinant Basin and the VSA became linked with the Campine Basin. The Souvré block subsided from the latest Givetian to the late Frasnian and recorded a thick middle Frasnian limestone series, but was later emergent, so much that karstic cavities developed. The Souvré block was submerged for a short time at the top of the Tournaisian, during the very high highstand (HST) corresponding to eustatic sequence 4, and the caves fi lled up with sediments. The Hermalle-sous-Argenteau and the Bombaye blocks, situated respectively west and east of the Souvré block, evolved in the same way during the late Devonian. They subsided slightly from the earliest Tournaisian to the late Viséan (Warnan- tian), but usually remained emergent and recorded deposits only during times of high eustatic levels corresponding to the early Tournaisian (for the Bombaye block), the end of the Tournaisian (HST of sequence 4), the end of the early Viséan (HST of sequence 6), then the late Viséan (HST of sequences 9 and 10). In its southern part, the Hermalle-sous-Argenteau block recorded also lowermost Viséan limestones correlated with the highstand of the eustatic sequence 5, probably as a result of the tilting of the block to the south at this time. [less ▲]

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See detailInteraction between hippocampal and striatal systems predicts subsequent consolidation of motor sequence memory.
Albouy, Geneviève; Sterpenich, Virginie; Vandewalle, Gilles ULg et al

in PLoS ONE (2013), 8(3), 59490

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See detailInteraction between human papillomavirus (HPV) and natural killer (NK) cells
Renoux, Virginie ULg; Longton, Laurence; Dortu, Estelle ULg et al

Conference (2007, October 10)

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See detailInteraction between modal domains: The case of the Subjunctive in Late Egyptian
Polis, Stéphane ULg

Conference (2010, March 09)

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See detailInteraction between monobactams and model D-alanyl-D-alanine-cleaving peptidases
Frère, Jean-Marie ULg; Klein, Daniel; Kelly, Judith A et al

in FEMS Microbiology Letters (1984), 21

Several monobactams reacted with the serine dd-peptidases of Streptomyces R61 and Actinomadura R39 in a manner similar to that of bicyclic penicillins and cephalosporins. The dissociation constants of the ... [more ▼]

Several monobactams reacted with the serine dd-peptidases of Streptomyces R61 and Actinomadura R39 in a manner similar to that of bicyclic penicillins and cephalosporins. The dissociation constants of the Michaelis complexes formed between the R61 enzyme and sulfazecin (32 μM) and between the R39 peptidase and SQ 26324 (0.35 μM) had the lowest values ever observed with any β-lactam compound, suggesting an excellent fit of these two monobactams with the active sites of the respective enzymes. Azthreonam had a very poor inactivating potency, confirming its high selective reactivity towards the penicillin binding protein No. 3 of Escherichia coli. The Zn2+dd-peptidase (from Streptomyces albus G) had a high intrinsic resistance to β-lactam compounds whether they possessed a mono- or a bicyclic structure. [less ▲]

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