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See detailInter-species differences for polychlorinated biphenyls and polybrominated diphenyl ethers in marine top predators from the Southern North Sea: Part 2. Biomagnification in harbour seals and harbour porpoises
Weijs, Liesbeth; Dirtu, Alin C; Das, Krishna ULg et al

in Environmental Pollution (2009), 157

Harbour porpoises (Phocoena phocoena) and harbour seals (Phoca vitulina) were found to differ in the ability to metabolize polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs ... [more ▼]

Harbour porpoises (Phocoena phocoena) and harbour seals (Phoca vitulina) were found to differ in the ability to metabolize polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). Biomagnification factors (BMFs), calculated between both predators and their prey (sole – Solea solea and whiting – Merlangius merlangus), had a large range of variation (between 0.5 and 91 for PCBs and between 0.6 and 53 for PBDEs). For the higher chlorinated PCBs and the highest brominated PBDEs, the BMF values in adult males were significantly higher than in the juvenile individuals of both species. BMF values of hexa- to octa-PCBs were the highest, suggesting reduced ability to degrade these congeners. Harbour porpoises had higher BMFs for lower chlorinated PCBs and for all PBDEs compared to harbour seals. Other factors, which may influence biomagnification, such as the octanol–water partition coefficients and the trophic level position measured through stable isotope (d15N) analysis, were found to be of lesser importance to predict biomagnification in the studied food chain. [less ▲]

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See detailInterações entre professores e alunos em atividades esportivas selecionadas: o aluno como ponto de partida
Piéron, Maurice; Cloes, Marc ULg

in ArtUS (1981), 9/11

In physical education, many studies of the teaching process started by analysing verbal interactions between teacher and students. Observationsal systems used in classroom were applied as such in physical ... [more ▼]

In physical education, many studies of the teaching process started by analysing verbal interactions between teacher and students. Observationsal systems used in classroom were applied as such in physical education setting or were modified to better suit the specific aspects of the subject matter. The image provided by interaction analysis is supposed to reflect the climate of the class. ... [less ▲]

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See detailThe Interacting Early-Type Binary BD +40°4220 (V729 Cyg): Modeling the Colliding Winds Region
Rauw, Grégor ULg; Vreux, Jean-Marie ULg; Bohannan, B.

in Astrophysical Journal (1999), 517

We present an analysis of an extensive set of spectroscopic observations of the mysterious early-type binary BD +40 deg4220. A new orbital solution is derived from the radial velocities of the absorption ... [more ▼]

We present an analysis of an extensive set of spectroscopic observations of the mysterious early-type binary BD +40 deg4220. A new orbital solution is derived from the radial velocities of the absorption lines. We confirm that the secondary star is highly overluminous for its mass. The absorption lines of both components display phase-locked profile variations, with some of the secondary's lines going into emission between phi=0.20 and phi=0.55. A detailed investigation of the profile variability of the He II lambda4686 emission line reveals that the pattern of variability of this line is very stable. We show that part of the He II lambda4686 emission is produced in the wind interaction region between the stars. Most of the emission lines in the visual spectrum of BD +40 deg4220 display variations that are reminiscent of those observed on the He II lambda4686 line, pointing toward a similar origin. We present numerical simulations of emission-line profiles in a colliding winds binary, showing that an important part of the variability observed in BD +40 deg4220 can be explained by a colliding winds phenomenon. The properties of the wind interaction region can be accounted for if we assume that the secondary star is an evolved object, most probably some kind of Ofpe/WN9 transition star, with a mass loss rate of ~5.5x10^-6 M_solar yr^-1. We finally discuss the fundamental parameters of the binary, concluding that mass transfer must have played a crucial role in the evolution of this system. [less ▲]

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See detailInteracting elementary excitations in ultrarelativistic plasmas: Damping mechanisms and plasma thermodynamics
Vanderheyden, Benoît ULg

Doctoral thesis (1998)

This dissertation examines the properties of the elementary excitations in quark-gluon and electromagnetic plasmas. Due to Debye screening and Landau damping mechanisms, the interaction fields fall off at ... [more ▼]

This dissertation examines the properties of the elementary excitations in quark-gluon and electromagnetic plasmas. Due to Debye screening and Landau damping mechanisms, the interaction fields fall off at large distances, except for static magnetic and color-magnetic fields. The lack of screening of the magnetic interaction leads to a singularity at small momentum transfer in the matrix element for fermion-fermion scattering via the exchange of a single gluon, or a single photon. We analyze the effects of this singularity on the quasiparticle lifetimes in plasmas at zero and at finite temperature T. First, at T = 0, we find that the fermion excitations above the ground state are long-lived, as the Pauli exclusion principle limits the collision phase space. Because the magnetic interaction is long-ranged, the damping rates of the excitations near the edge of the Fermi sea vary linearly with the excitation energy. The quasiparticles are therefore not as well- defined as those encountered in non-relativistic plasmas, with short-ranged interactions, where the variation of the damping rates away from the Fermi surface is quadratic, thus slower. Second, we find that in plasmas at finite temperatures, the magnetic interaction introduces correlations between the successive scatterings of the fermion quasiparticles on the charges in the system. This effect leads to a decay law that decreases in time more rapidly than an exponential; however the quasiparticles are long-lived. We also derive a framework for ascertaining the effects of the long-ranged magnetic interaction on the thermodynamic properties of relativistic plasmas. Our method generalizes the concept of conserving, Φ-derivable, approximations to relativistic field theories. By treating the interaction field as a dynamical degree of freedom, we are able to derive the thermodynamical potential in terms of full propagators. This approach allows us to resolve the entropy into contributions from its interacting elementary excitations. [less ▲]

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See detailInteracting surface of the receptor-binding domain.
GATOT, Jean-Stéphane ULg; Callebaut, Isabelle; Van Lint, Carine et al

in Société Belge de Biochimie et de Biologie moléculaire. (2002, February 22)

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See detailInteracting winds in massive binaries
Rauw, Grégor ULg

in Pavlovski, Kresimir; Tkachenko, Andrew; Torres, Guillermo (Eds.) EAS Publications Series (2013, February 01)

Massive stars feature highly energetic stellar winds that interact whenever two such stars are bound in a binary system. The signatures of these interactions are nowadays found over a wide range of ... [more ▼]

Massive stars feature highly energetic stellar winds that interact whenever two such stars are bound in a binary system. The signatures of these interactions are nowadays found over a wide range of wavelengths, including the radio domain, the optical band, as well as X-rays and even γ-rays. A proper understanding of these effects is thus important to derive the fundamental parameters of the components of massive binaries from spectroscopic and photometric observations. [less ▲]

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See detailThe interaction between a protein and a ligand molecule studied by linear and non-linear optical spectroscopies
Dreesen, Laurent ULg; Méthivier, C.; Sartenaer, Y. et al

in Physical Chemical News (2007), 36

We use infrared reflection-adsorption (IRRAS) and sum-frequency generation (SFG) spectroscopies to study the interaction between a protein (avidin) and a ligand molecule (biocytin or its thiol derivative ... [more ▼]

We use infrared reflection-adsorption (IRRAS) and sum-frequency generation (SFG) spectroscopies to study the interaction between a protein (avidin) and a ligand molecule (biocytin or its thiol derivative) adsorbed on metals (Au and Ag) and insulators (CaF2 in two separate spectral ranges 2800-3500 cm-1 and 1400-1800 cm-1, respectively. No specific interaction is detected by SFG when the measurements are carried out on metals although IRRAS measurements attest the presence of a protein ad-layer. This is explained by the disordered character of the adsorbed film of avidin which is therefore SFG inactive. When the experiments are performed on an insulating substrate, no change of the biocytin/CaF2 SFG spectrum is detected in the low spectral range (1400-1800 cm-1) after immersion in an avidin solution in contrast with what was clearly demonstrated in the 2800-3500 cm-1 spectral range. Finally, we report, for the first time, the observation by SFG of the CH2 scissor vibration mode at 1465 cm-1. [less ▲]

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See detailInteraction between antimicrobial peptides and membranes
Deleu, Magali ULg

Conference (2004)

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See detailInteraction between Antitumor Drugs and a Double-Stranded Oligonucleotide Studied by Electrospray Ionization Mass Spectrometry
Gabelica, Valérie ULg; De Pauw, Edwin ULg; Rosu, Frédéric ULg

in Journal of Mass Spectrometry [=JMS] (1999), 34(12), 1328-1337

Electrospray ionization mass spectrometry was used to investigate the complex formation between a double-stranded oligonucleotide and various antitumor drugs belonging to two categories: intercalators ... [more ▼]

Electrospray ionization mass spectrometry was used to investigate the complex formation between a double-stranded oligonucleotide and various antitumor drugs belonging to two categories: intercalators (ethidium bromide, amsacrine and ascididemin) and minor groove binders (Hoechst 33258, netropsin, distamycin A, berenil and DAPI). The goal of this study was to determine whether the relative intensities in the mass spectra reflect the relative abundances of the species in the solution phase. The full-scan mass spectra suggest non-specific binding for the intercalators and specific binding for the minor groove binders. The preferential stoichiometries adopted by each minor groove binder were determined by studying the influence of the drug concentration on the spectra. We obtained 2:1 > 1:1 for distamycin, 1:1 > 2:1 for Hoechst 33258 and DAPI and only the 1 : 1 complex for netropsin and berenil. These features reflect their known behavior in solution. The compared tandem mass spectra of the 1 : 1 complexes with Hoechst 33258 and netropsin, when correlated with published crystallographic data, suggest the possibility of inferring some structural information. The relative binding affinities of the drug for the considered duplex were deduced with two by two competition experiments, assuming that the relative intensities reflect the composition of the solution phase. The obtained affinity scale is netropsin > distamycin A > DAPI > Hoechst 33258 > berenil. These examples show some of the potential uses of mass spectrometry as a useful tool for the characterization of specific drug binding to DNA, and possibly a rapid drug screening method requiring small amounts of materials. [less ▲]

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See detailInteraction between ascites susceptibility and CO during the second half of incubation of two broiler lines. Effect on embryonic development and hatching process.
Everaert, Nadia ULg; Debonne, M.; Willemsen, H. et al

in British poultry science (2010), 51(3), 335-43

1. Because CO(2) during the second half of incubation is known to influence air cell and blood gases, and embryo development, it is postulated that post-hatch development and ascites sensitivity could ... [more ▼]

1. Because CO(2) during the second half of incubation is known to influence air cell and blood gases, and embryo development, it is postulated that post-hatch development and ascites sensitivity could also be influenced. 2. An ascites susceptible (A) and an ascites resistant (E) broiler line were incubated under standard incubation or high CO(2) conditions (up to 4%) from embryonic day (ED) 10 onwards. The embryonic development and the hatching process of these two lines were compared when incubated under standard or high CO(2) conditions from over the second half of incubation. 3. The A line, selected for high post-hatch growth rate, exhibited a higher relative embryo weight from ED10 until ED16, which was supported by a higher air cell pCO(2), lower air cell pO(2), higher corticosterone and thyroid hormones and earlier hatching time. 4. Incubation under high CO(2) increased air cell pCO(2), retarded yolk consumption, and decreased glycogen concentration in the liver at hatch. Hatchability decreased in both lines when incubated under high CO(2), due to an increased late mortality of embryos that died before IP. 5. These results suggest that the development and metabolism of CO(2)-incubated embryos differ from control incubated embryos. [less ▲]

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See detailInteraction between beta-lactam antibiotics and exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61
Frère, Jean-Marie ULg; Leyh-Bouille, Mélina; Ghuysen, Jean-Marie ULg et al

in European Journal of Biochemistry (1974), 50(1), 203-214

On the basis of steady-state kinetics, inhibition of the exocellular dd-carboxypeptidase-trans-peptidase of Streptomyces R61 by β-lactam antibiotics was competitive with regard to the donor substrate ... [more ▼]

On the basis of steady-state kinetics, inhibition of the exocellular dd-carboxypeptidase-trans-peptidase of Streptomyces R61 by β-lactam antibiotics was competitive with regard to the donor substrate. However, the complexes formed between the Streptomyces R61 enzyme and various β-lactam antibiotics were relatively stable, exhibiting half-lives of 40 to 80 min at 37°C and neutral pH. During breakdown of the complexes the protein underwent reactivation, whereas the released antibiotic molecule was chemically altered. With [14C]benzylpenicillin, the released compound was neither benzylpenicillin nor benzylpenicilloic acid. The properties of the Streptomyces R61 enzyme β-lactam antibiotic complexes were compared with those of the complexes formed between the same antibiotics and either the membrane-bound transpeptidase from Streptomyces R61 or the exocellular dd-carboxypeptidase-transpeptidase of Streptomyces R39. [less ▲]

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See detailThe interaction between Bovine Leukemia Virus and its target cell.
Burny, Arsène; Kettmann, Richard ULg; Willems, Luc ULg et al

in AIDS Research and Human Retroviruses (1992), 8

Previous results indicate that the external glycoprotein gp51 of bovine leukemia virus plays an important role in the process of cell fusion induced by bovine leukemia virus (Bruck, C., Mathot, S ... [more ▼]

Previous results indicate that the external glycoprotein gp51 of bovine leukemia virus plays an important role in the process of cell fusion induced by bovine leukemia virus (Bruck, C., Mathot, S., Portetelle, D., Berte, C., Franssen, J. D., Herion, P., and Burny, A. (1982) Virology 122, 342-352; Voneche, V., Portetelle., D., Kettmann, R., Willems, L., Limbach, K., Paoletti, E., Ruysschaert, J. M., Burny, A., and Brasseur, R. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 3810-3814) and suggest that a region encompassing residues 23 and 25 of gp51 is involved in this process (Portetelle, D., Couez, D., Bruck, C., Kettmann, R., Mammerickx, M., Van der Maaten, M., Brasseur, R., and Burny, A. (1989) Virology 169, 27-33; Mamoun, R., Morisson, M., Rebeyrotte, N., Busetta, B., Couez, D., Kettmann, R., Hospital, M., and Guillemain, B. (1990) J. Virol. 64, 4180-4188). X-ray diffraction studies performed on envelope glycoproteins of influenza virus indicate that the NH2-terminal part of the external glycoprotein lies very close to the fusion peptide. The same overall structure seems to exist in human immunodeficiency virus as suggested by site-directed mutagenesis followed by syncytia induction assays. Our theoretical studies indicate that a segment expanding between residues 19 and 27 of gp51 probably adopts an amphipathic beta-strand structure. We hypothesize that the amphipathic 19-27 structure of gp51 plays an important role in the process of membrane fusion by interacting with the fusion peptide or with another region of gp30. Mutational analysis disrupting the amphipathy of the 19-27 region strongly altered the fusogenic capacity of the gp51-gp30 complex. [less ▲]

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See detailInteraction between Class B Beta-Lactamases and Suicide Substrates of Active-Site Serine Beta-Lactamases
Prosperi-Meys, C.; Llabres, Gabriel ULg; De Seny, Dominique ULg et al

in FEBS Letters (1999), 443(2), 109-11

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders ... [more ▼]

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders of magnitude. A comparison of the kinetic parameters for the alpha and beta isomers of 6-iodopenicillanic acid shows that there is no general preference for the alpha isomer and that the efficient hydrolysis of imipenem by these enzymes must rest on other factors. [less ▲]

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See detailInteraction between dendritic cells and nerve fibres in lymphoid organs after oral scrapie exposure
Dorban, Gauthier ULg; Defaweux, Valérie ULg; Demonceau, Christine ULg et al

in Virchows Archiv (2007), 451(6), 1057-1065

In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting ... [more ▼]

In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting the nervous system. To clarify the cellular requirements for the neuro-invasion of the scrapie agent from the lymphoid organs to the central nervous system, we have studied, by confocal microscopy, the innervations within Peyer's patches, mesenteric lymph nodes and the spleen of mice in physiological conditions and after oral exposure to prion. Contacts between nerve fibres and PrPsc-associated cells, dendritic cells (DCs) and follicular dendritic cells (FDCs), were evaluated in preclinical prion-infected mice. Using a double immunolabelling strategy, we demonstrated the lack of innervation of PrPsc-accumulating cells (FDCs). Contacts between nerve fibers and PrPsc-propagating cells (DCs) were detected in T-cell zones and cell-trafficking areas. This supports, for the first time, the possible implication of dendritic cells in the prion neuroinvasion process. [less ▲]

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See detailInteraction between dietary protein content and the source of carbohydrates along the gastrointestinal tract of weaned piglets.
Pieper, Robert; Boudry, Christelle ULg; Bindelle, Jérôme ULg et al

in Archives of Animal Nutrition (2014), 68

Although fermentable carbohydrates (CHO) can reduce metabolites derived from dietary protein fermentation in the intestine of pigs, the interaction between site of fermentation and substrate availability ... [more ▼]

Although fermentable carbohydrates (CHO) can reduce metabolites derived from dietary protein fermentation in the intestine of pigs, the interaction between site of fermentation and substrate availability along the gut is still unclear. The current study aimed at determining the impact of two different sources of carbohydrates in diets with low or very high protein content on microbial metabolite profiles along the gastrointestinal tract of piglets. Thirty-six piglets (n = 6 per group) were fed diets high (26%, HP) or low (18%, LP) in dietary protein and with or without two different sources of carbohydrates (12% sugar beet pulp, SBP, or 8% lignocellulose, LNC) in a 2 × 3 factorial design. After 3 weeks, contents from stomach, jejunum, ileum, caecum, proximal and distal colon were taken and analysed for major bacterial metabolites (D-lactate, L-lactate, short chain fatty acids, ammonia, amines, phenols and indols). Results indicate considerable fermentation of CHO and protein already in the stomach. HP diets increased the formation of ammonia, amines, phenolic and indolic compounds throughout the different parts of the intestine with most pronounced effects in the distal colon. Dietary SBP inclusion in LP diets favoured the formation of cadaverine in the proximal parts of the intestine. SBP mainly increased CHO-derived metabolites such as SCFA and lactate and decreased protein-derived metabolites in the large intestine. Based on metabolite profiles, LNC was partly fermented in the distal large intestine and reduced mainly phenols, indols and cadaverine, but not ammonia. Multivariate analysis confirmed more diet-specific metabolite patterns in the stomach, whereas the CHO addition was the main determinant in the caecum and proximal colon. The protein level mainly influenced the metabolite patterns in the distal colon. The results confirm the importance of CHO source to influence the formation of metabolites derived from protein fermentation along the intestinal tract of the pig. [less ▲]

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See detailInteraction between dietary protein level and source of fermentable carbohydrates in the gastrointestinal tract of pigs
Pieper, Robert; Boudry, Christelle ULg; Vahjen, Wilfried et al

in International workshop on nutrition and intestinal microbiota host interaction in the pig : Book of abstracts (2013, October 24)

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See detailInteraction between earthworms and soil fungi: volatiles attraction
Zirbes, Lara ULg; Thonart, Philippe ULg; Wathelet, Jean-Paul ULg et al

in 9th International Symposium on Earthworm Ecology (2010)

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