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See detailIn vivo PET/CT in a human glioblastoma chicken chorioallantoic membrane model: A new tool for oncology and radiotracer development.
Warnock, Geoffrey; Turtoi, Andrei ULg; Blomme, Arnaud ULg et al

in Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine (2013), 54(10), 1782-1788

For many years the laboratory mouse has been used as the standard model for in vivo oncology research, particularly in the development of novel PET tracers, but the growth of tumors on chicken ... [more ▼]

For many years the laboratory mouse has been used as the standard model for in vivo oncology research, particularly in the development of novel PET tracers, but the growth of tumors on chicken chorioallantoic membrane (CAM) provides a more rapid, low cost and ethically sustainable alternative. For the first time, we demonstrate the feasibility of in vivo PET and CT imaging in a U87 glioblastoma tumor model on chicken chorioallantoic membrane (CAM), with the aim of applying this model for screening of novel PET tracers. Methods: U87 glioblastoma cells were implanted on the CAM at day 11 post-fertilization and imaged at day 18. A small animal imaging cell was used to maintain incubation and allow anesthesia using isoflurane. Radiotracers were injected directly into the exposed CAM vasculature. Sodium [18F]fluoride was used to validate the imaging protocol, demonstrating that image-degrading motion can be removed with anesthesia. Tumor glucose metabolism was imaged using [18F]fluorodeoxyglucose and tumor protein synthesis was imaged using 2-[18F]fluoro-L-tyrosine. Anatomical images were obtained by contrast enhanced CT, facilitating clear delineation of the tumor, delineation of tracer uptake in tumor versus embryo and accurate volume measurements. Results: PET imaging of tumor glucose metabolism and protein synthesis was successfully demonstrated in the CAM U87 glioblastoma model. Catheterization of CAM blood vessels facilitated dynamic imaging of glucose metabolism with [18F]fluorodeoxyglucose and demonstrated the ability to study PET tracer uptake over time in individual tumors, while CT imaging improved the accuracy of tumor volume measurements. Conclusion: In summary, we describe the novel application of PET/CT in the CAM tumor model, with optimization of typical imaging protocols. PET imaging in this valuable tumor model could prove particularly useful for rapid, high-throughput screening of novel radiotracers. [less ▲]

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See detailIn vivo protection of spiral ganglion neurons by bryostatin 1: preliminary results
POIRRIER, Anne-Lise ULg; Van Den Ackerveken, Priscilla ULg; Defourny, Jean et al

in Advances in Cellular and Molecular Otolaryngology (2013), 1

Background: We aim to demonstrate the effect of bryostatin 1, a macrocyclic lactone that activates protein kinase C, on spiral ganglion neurons (SGNs) of adult guinea pigs deafened by aminoglycoside ... [more ▼]

Background: We aim to demonstrate the effect of bryostatin 1, a macrocyclic lactone that activates protein kinase C, on spiral ganglion neurons (SGNs) of adult guinea pigs deafened by aminoglycoside. Methodology: Twenty-one guinea pigs were deafened by the aminoglycoside gentamicin and then treated by continuous infusion of experimental molecule for 1 month. The experimental molecule was bryostatin 1, artificial perilymph (negative control), or neurotrophins and an apoptosis inhibitor (positive control). Neuronal density in the spiral ganglia was quantified. Results: Bryostatin 1 protected SGNs after a gentamicin challenge. Conclusions: Bryostatin 1 has a neuroprotective effect when administered continuously at low doses in adult guinea pigs. [less ▲]

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See detailIn vivo responsiveness of morphological variants of growth hormone-producing pituitary adenomas to octreotide
Ezzat, S.; Kontogeorgos, G.; Redelmeier, D. A. et al

in European Journal of Endocrinology (1995), 133

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See detailIn vivo ribosomal RNA turnover is down-regulated in leukaemic cells in chronic lymphocytic leukaemia
Defoiche, Julien; Zhang, Yan; Lagneaux, Laurence et al

in British Journal of Haematology (2010), 151

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See detailIn vivo skin fluorescence imaging in young Caucasian adults with early malignant melanomas
PIERARD, Gérald ULg; Hermanns-Lê, Trinh ULg; Pierard, Sébastien ULg et al

in Clinical, Cosmetic and Investigational Dermatology (2014), 7

Background: Human cutaneous malignant melanoma (CMM) is an aggressive cancer showing a dramatic worldwide increase in incidence over the past few decades. The most prominent relative epidemiological ... [more ▼]

Background: Human cutaneous malignant melanoma (CMM) is an aggressive cancer showing a dramatic worldwide increase in incidence over the past few decades. The most prominent relative epidemiological increase has been disclosed in young women. The aim of the study was to assess the effects of chronic sun exposures in order to rate the extend of melanocytic stimulations in the vicinity of CMM. Methods: The study was designed to evaluate the melanin distribution and density using ultraviolet light illumination. The present study was performed on surgical excision specimens of thin CMM lesion removed from the upper limbs of 55 Caucasian adults (37 women and 18 men). Two control groups comprised 23 men and 21 women of similar ages who had medium-size congenital melanocytic nevi, also present on the upper limbs. The peritumoral skin was scrutinized using a Visioscan® VC98 device, revealing the faint mosaic melanoderma (FMM) pattern that grossly indicates early signs of chronic photodamage in epidermal melanin units. Results: The median extent of relative FMM was significantly higher in the CMM male group. By contrast, the CMM female group showed a reverse bimodal distribution in FMM size. Only 12/37 (32.5%) of the CMM female group had an increased FMM size, whereas 25/37 (67.5%) of females with CMM had a global FMM extent in the normal range, relative to the controls. Conclusion: Thin CMM supervening in young women appear unrelated to repeat photoexposure. Other mechanisms are possibly involved. [less ▲]

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See detailIn vivo studies of p-[18F]MPPF metabolites in human.
Damhaut, Ph.; Plenevaux, Alain ULg; Lemaire, Christian ULg et al

in Journal of Labelled Compounds & Radiopharmaceuticals (1999), 42

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See detailIn vivo studies on the mononuclear phagocyte system Fc receptor function in rheumatoid arthritis. Correlations with clinical and immunological variables
Malaise, Michel ULg; Foidart, J. B.; Hauwaert, Christian ULg et al

in Journal of Rheumatology (1985), 12(1), 33-42

The mononuclear phagocyte system Fc receptor function was assessed in 13 control subjects and 17 patients with rheumatoid arthritis (RA) by intravenous injection of IgG coated (IgG-RBC) or of heat-damaged ... [more ▼]

The mononuclear phagocyte system Fc receptor function was assessed in 13 control subjects and 17 patients with rheumatoid arthritis (RA) by intravenous injection of IgG coated (IgG-RBC) or of heat-damaged (HD-RBC) 99rnTc-Iabeled autologous erythrocytes. Although the clearance half-times of control and RA erythrocytes were not significantly different, the spleen to liver uptake ratios per surface area (S/Ls), determined by quantitative scintigraphic analysis, were significantly lower in RA patients than in controls. The S/Lswere significantly correlated with the Steinbrocker stages Ir = 0.92; p < 0.01), the disease duration (r = 0.73; p < 0.01) and the total immunoglobulin levels (r = 0.73; p < 0.01). The Clq binding activity of the sera was inversely correlated with the spleen (r = 0.90; p < 0.01) and liver uptakes (r = 0.73; p < 0.02). Our results therefore show an alteration of the Fc receptor function of splenic and hepatic mononuclear phagocytes in RA patients. [less ▲]

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See detailIn vivo study of enhancing factors for guided bone formation
Biewer, Bob; Van Heusden, Alain ULg; Rompen, Eric ULg

in Journal of Clinical Periodontology (1997), 11

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See detailIn vivo study of enhancing factors for guided bone formation.
Biewer, Bob; Van Heusden, Alain ULg; Rompen, Eric ULg

Poster (1997)

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See detailIn vivo transfection of bovine leukemia provirus into sheep.
Willems, Luc ULg; Portetelle, Daniel ULg; Kerkhofs, P. et al

in Virology (1992), 189(2),

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See detailIn Vivo Tumorigenesis Was Observed after Injection of In Vitro Expanded Neural Crest Stem Cells Isolated from Adult Bone Marrow
Wislet, Sabine ULg; Poulet, Christophe ULg; Neirinckx, Virginie ULg et al

in PLoS ONE (2012), 7(10), 46425

Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several studies have reported that in vitro passaging of mesenchymal stem cells ... [more ▼]

Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several studies have reported that in vitro passaging of mesenchymal stem cells alters the functional and biological properties of those cells, leading to the accumulation of genetic aberrations. Recent studies described bone marrow stromal cells (BMSC) as mixed populations of cells including mesenchymal (MSC) and neural crest stem cells (NCSC). Here, we report the transformation of NCSC into tumorigenic cells, after in vitro long-term passaging. Indeed, the characterization of 6 neural crest-derived clones revealed the presence of one tumorigenic clone. Transcriptomic analyses of this clone highlighted, among others, numerous cell cycle checkpoint modifications and chromosome 11q down-regulation (suggesting a deletion of chromosome 11q) compared with the other clones. Moreover, unsupervised analysis such as a dendrogram generated after agglomerative hierarchical clustering comparing several transcriptomic data showed important similarities between the tumorigenic neural crest-derived clone and mammary tumor cell lines. Altogether, it appeared that NCSC isolated from adult bone marrow represents a potential danger for cellular therapy, and consequently, we recommend that phenotypic, functional and genetic assays should be performed on bone marrow mesenchymal and neural crest stem cells before in vivo use, to demonstrate whether their biological properties, after ex vivo expansion, remain suitable for clinical application. [less ▲]

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See detailIn vivo, in vitro, in silico: computational tools for tissue engineering
Van Oosterwyck, Hans; Truscello, S.; Demol, Jan et al

in proceedings of the 2nd International Conference on Innovation for Sustainable Production i-SUP (2010)

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See detail(In)sécurités linguistiques francophones en Belgique
Dassargues, Alix ULg

Conference (2013, May 15)

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See detail(In)sécurités linguistiques francophones en Belgique : du taux d’insécurité aux facteurs d’(in)sécurité
Dassargues, Alix ULg

in L'insécurité en question : défis, enjeux et perspectives (2015, March)

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See detail(In)visible Bodies: Crossing Racial Lines in the Graphic Novel
Dony, Christophe ULg

Conference (2011)

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See detail(In)Visible Bodies: Rewriting the Politics of Passing in Incognegro, a Graphic Mystery by Mat Johnson and Warren Pleece
Dony, Christophe ULg

Conference (2013, January 03)

How can passing across racial lines be described and conveyed in the comics form? Can the medium develop specific strategies to comment on the themes of transgression and crossing inherent to the trope of ... [more ▼]

How can passing across racial lines be described and conveyed in the comics form? Can the medium develop specific strategies to comment on the themes of transgression and crossing inherent to the trope of passing? This paper shows how the graphic novel Incognegro (2008) goes beyond the traditional socio-historical analysis of passing and plays thematically, generically, and visually with the politics of the trope. Incognegro, set in the US in the early 1930s, depicts a light-skinned African American reporter who passes for white in order to investigate lynchings of blacks in the deep South. Because white papers do not consider these events to be news, the reporter condemns these dreadful acts in the column titled “Incognegro” that he writes for a New York-based newspaper, The New Holland Herald. Thus he risks his life using his “passing abilities” to protect the rights of the community he is trying to defend from white hegemony. In this way, Incognegro challenges the conventional tragic mulatto figure that passes for white to avoid racism and violence or to improve his/her social status. In addition, it echoes the common black trickster figure who practices “masking” to outwit his enemies or opponents, and calls into question the biological, social, and cultural representations of race as perceived by the white dominant group. Here the racial passer functions as an “outsider from within” who can challenge the black vs. white binary model. Moreover, the text subverts narrative paradigms from superhero comics and detective fiction to point out the arbitrariness of racial ideologies and the fallibility of notions of justice and truth. This subversion is further complicated by the comic’s “color free” art (black and white, sans halftones or gray tones), which defies essentialist representations of race. In sum, Incognegro argues that the ambiguities of racial categorization are best represented through a similarly ambiguous literary genre—one that not only challenges generic norms and traditions but also “writes back” to both the mainstream and alternative wings of American comics production. [less ▲]

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See detailIn-capillary derivatization with (-)-1-(9-fluorenyl)ethyl chloroformate as chiral labeling agent for the electrophoretic separation of amino acids.
Fradi, Ines ULg; Farcas, Elena ULg; Said, Azza Ben et al

in Journal of chromatography. A (2014), 1363

An original micellar electrokinetic chromatography (MEKC) method using in-capillary derivatization with a chiral labeling reagent was developed for the separation of amino acid (AA) derivatives. The ... [more ▼]

An original micellar electrokinetic chromatography (MEKC) method using in-capillary derivatization with a chiral labeling reagent was developed for the separation of amino acid (AA) derivatives. The potential of (-)-1-(9-fluorenyl)-ethyl chloroformate (FLEC) as in-capillary derivatization agent is described for the first time. Several parameters for in-capillary derivatization and subsequent MEKC separation were systematically investigated using experimental designs. Firstly experimental conditions for in-capillary derivatization were optimized using face-centered central composite design (FCCD). Mixing voltage and time as well as concentration of the labeling solution were investigated. Efficient labeling was achieved by sequential injection of AAs and FLEC labeling solution followed by the application of a voltage of 0.2kV for 570s. The background electrolyte (BGE) composition was then optimized in order to achieve selectivity. A FCCD was performed with two factors, namely the sodium dodecyl sulfate (SDS) concentration and the percentage of propan-2-ol (IPA). The separation of 12 pairs of derivatized AA (FLEC-AA) diastereomers was achieved with resolution values comprised between 3 and 20. Furthermore, an efficient derivatization and separation of 29 FLEC-AA derivatives were achieved in a single run using a buffer made up of 40mM sodium tetraborate, 21mM SDS and 8.5% IPA. The method was successfully applied to the analysis of spiked artificial cerebrospinal fluid (aCSF) sample. [less ▲]

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See detailIn-depth phenotyping of a Donnai-Barrow patient helps clarify proximal tubule dysfunction.
Dachy, Angelique; Paquot, Francois; Debray, Guillaume et al

in Pediatric nephrology (Berlin, Germany) (2015), 30(6), 1027-31

BACKGROUND: The megalin/cubilin/amnionless complex is essential for albumin and low molecular weight (LMW) protein reabsorption by renal proximal tubules (PT). Mutations of the LRP2 gene encoding megalin ... [more ▼]

BACKGROUND: The megalin/cubilin/amnionless complex is essential for albumin and low molecular weight (LMW) protein reabsorption by renal proximal tubules (PT). Mutations of the LRP2 gene encoding megalin cause autosomal recessive Donnai-Barrow/facio-oculo-acoustico-renal syndrome (DB/FOAR), which is characterized by LMW proteinuria. The pathophysiology of DB/FOAR-associated PT dysfunction remains unclear. CLINICAL CASE: A 3-year-old girl presented with growth retardation and proteinuria. Clinical examination was unremarkable, except for a still-opened anterior fontanel and myopia. Psychomotor development was delayed. At 6, she developed sensorineural hearing loss. Hypertelorism was noted when she turned 12. Blood analyses, including renal function parameters, were normal. Urine sediment was bland. Proteinuria was significant and included albumin and LMW proteins. Immunoblotting analyses detected cubilin and type 3 carbonic anhydrase (CA3) in the urine. Renal ultrasound was unremarkable. Optical examination of a renal biopsy did not disclose any tubular or glomerular abnormality. Electron microscopy revealed that PT apical endocytic apparatus was significantly less developed. Immunostaining for megalin showed a faint signal in PT cytosol contrasting with the distribution of cubilin at the apical membrane. The diagnostic procedure led to identifying two mutations of the LRP2 gene. CONCLUSIONS: The functional loss of megalin in DB/FOAR causes PT dysfunction characterized by increased urinary shedding of CA3 and cubilin. [less ▲]

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See detailIN-FLIGHT PERFORMANCE OF THE SOLAR UV RADIOMETER LYRA / PROBA-2
Stockman, Yvan ULg; Defise, Jean-Marc ULg; Halain, Jean-Philippe ULg et al

(2010, October 05)

LYRA is a solar radiometer, part of the PROBA-2 micro-satellite payload. The PROBA-2 mission has been launched on 02 November 2009 with a Rockot launcher to a Sun-synchronous orbit at an altitude of 725 ... [more ▼]

LYRA is a solar radiometer, part of the PROBA-2 micro-satellite payload. The PROBA-2 mission has been launched on 02 November 2009 with a Rockot launcher to a Sun-synchronous orbit at an altitude of 725 km. Its nominal operation duration is two years with possible extension of 2 years. LYRA monitors the solar irradiance at a high cadence (> 20Hz) in four soft X-Ray to VUV large passbands: the “Lyman-Alpha” channel, the “Herzberg” continuum range, the “Aluminium” and “Zirconium” filter channels. The radiometric calibration is traceable to synchrotron source standards. LYRA benefits from wide bandgap detectors based on diamond. It is the first space assessment of these revolutionary UV detectors for astrophysics. [less ▲]

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